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| 1 | cDNA-AFLP结合BSA研究马铃薯晚疫病菌小种特异无毒基因差异表达片段显示文摘通过对具有 6个无毒基因不同表型的马铃薯晚疫病菌构建的 4个混合池进行cDNA AFLP分析 ,得到与 6个与无毒基因相关的差异表达片段 (TDFs) 85条 ,其中与无毒基因Avr1相关的为 2 0条 ,Avr2 2 8条 ,Avr3 Avr10 Avr1116条 ,Avr4 2 1条。差异表达片段大小主要分布在 10 0bp和 30 0bp之间 ,最小片段为 6 0bp ,最大片段 4 40bp。 | 郭军 屈冬玉 王晓武 金黎平 谢开云 Rays Jiang Francine Govers | 2004 | 中国马铃薯2004,18,1: | 12 |
| 2 | 与马铃薯晚疫病菌无毒基因Avr1连锁的AFLP标记显示文摘以具有和不具有无毒基因Avr1表现型的马铃薯晚疫病菌株80029(AVR1)和88133(avr1)以及其杂交F1代50个菌株群体为试材,限制性内切酶PstI和HhaI为酶切组合,采用荧光AFLP技术和混合分组分析法(BSA),通过256对引物筛选得到了5个与Avr1连锁的候选AFLP标记并进行了菌株个体验证,遗传分析表明5个标记中有2个标记与Avr1连锁且在F1代中共分离,无交换重组发生。 | 郭军 Rob Weide 屈冬玉 王晓武 谢开云 Francine Govers | 2005 | 中国农业科学2005,38,9: | 10 |
| 3 | 马铃薯晚疫病菌小种特异无毒基因候选表达序列的cDNA-AFLP鉴定显示文摘利用具有6个无毒基因不同表现型且处于萌发中的静孢子时期的马铃薯晚疫病菌构建4个混 合池,通过cDNA AFLP分析,共获得85个与无毒基因相关的差异表达片段,其中与无毒基因Avr1、Avr2、 Avr3 Avr10 Avr11、Avr4相关的差异表达片段分别为20,28,16和21个。将这些差异表达片段对20个晚疫 病病菌个体进行无毒基因差异表达验证,得到1个无毒基因连锁群Avr3 Avr10 Avr11的候选片段,即 A11T13Avr3 10 11S157;无毒基因Avr4的候选片段2个,即A24T19Avr4S122和A24T24Avr4S142。Blast分析 表明:A11T13Avr3 10 11S157与马铃薯晚疫病菌萌发孢子囊(germinatingsporangium)EST(expressed sequencetag)库中的序列PG001F10.XT7同源;A24T19Avr4S122与晚疫病菌萌发中静孢子(germinating cyst)EST库中的PH051G10.XT7部分序列完全一致,而A24T24Avr4S142与PH051G10.XT7具有98%的 同源性。这些结果将促进通过电子克隆并结合RACE(RapidAmplificationofcDNAEnds)技术获得全长的无 毒基因Avr4。 | 郭军 屈冬玉 王晓武 金黎平 谢开云 Rays H.Y.Jiang Francine Govers | 2005 | 园艺学报2005,32,1: | 6 |
| 4 | An exploratory study on the use of enzyme activities as sediment tracers:biochemical fingerprints?显示文摘Little information exists on the potential of soil enzyme activities,which are sensitive to soil properties and management,for the characterization of sediment sources at the catchment scale.The objective of this study is to explore and evaluate enzyme activity as tracer for sediment fingerprinting in the Hiv catchment(55 km^2),Iran.Therefore,four enzymes were measured from 42 different sampling sites,covering three sediment source areas(rangeland/surface erosion,orchard/surface erosion,and streambank erosion),as well as from 12 sediment samples from reservoir check dams (sediment sinks).The results indicate that,based upon backward mode discriminant analysis,βglucosidase and dehydrogenase,allowed more than 95%of the samples to be correctly assigned to their source areas.These enzymes were selected as input data for a mixing-model to determine the relative contribution of the sampled sediment sources.The mean contributions from rangeland, orchard and streambank sources in the study area were estimated as 11.3%,15.1%and 73.7%, respectively.Using geochemical tracers,the mean contribution from rangeland,orchard and streambank sources was estimated as 14.1%,9.5%and 74.8%,respectively.Combined biochemical and geochemical tracers,similar values were obtained(18.7%,10.7%and 70.7%,respectively).Our results indicate that soil enzyme activity allows for a good characterization of sediment sources,and can provide a complementary tool to currently existing sediment fingerprinting approaches.However, the method should be also tested in other regions. | Kazem NOSRATI Gerard GOVERS Hassan AHMADI Forood SHARIFI Mohammad Ali AMOOZEGAR Roel MERCKX Matthias VANMAERCKE | 2011 | International Journal of Sediment Research2011,26,2: | 5 |
| 5 | 目的地品牌,还是地方品牌?显示文摘为进一步提高本刊的学术水平和国际影响力,本栏目2013年起进行了全新改版,特邀请相关领域的专家学者担任本栏目的特邀责任编辑,由这些特邀责任编辑约请各自领域国内外有一定影响力的专家学者参与讨论。 | Robert Govers 桂颖 | 2013 | 旅游学刊2013,28,1: | 2 |
| 6 | Review of the malaria epidemiology and trends in Zambia显示文摘A comprehensive desk review of malaria trends was conducted between 2000-2010 in Zambia to study malaria epidemiology and trends to guide strategies and approaches for effective malaria control.This review considered data from the National Health Information Management System,Malaria Surveys and Programme Review reports and analyzed malaria in-patient cases and deaths in relation to intervention coverage for all ages.Data showed three distinct epidemiological strata after a notable malaria reduction(66%)in in-patient cases and deaths,particularly between 2000-2008.These changes occurred following the(re-)introduction and expansion of indoor residual spraying up to 90%coverage,scale-up of coverage of long-lasting insecticidetreated nets in household from 50%to 70%,and artemisin-based combination therapy nationwide.However,malaria cases and deaths re-surged,increasing in 2009-2010 in the northern-eastern parts of Zambia.Delays in the disbursement of funds affected the implementation of interventions,which resulted in resurgence of cases and deaths.In spite of a decline in malaria disease burden over the past decade in Zambia,a reversal in impact is notable in the year 2009-2010,signifying that control gains are fragile and must be sustained toeliminate malaria. | Freddie Masaninga Emmanuel Chanda Pascalina Chanda-Kapata Busiku Hamainza Hieronymo T Masendu Mulakwa Kamuliwo Wambinji Kapelwa John Chimumbwa John Govere Mac Otten Ibrahima Soce Fall Olusegun Babaniyi | 2013 | Asian Pacific Journal of Tropical Biomedicine2013,3,2: | 2 |
| 7 | The control of nonstoichiometry in lithium nickel - cobalt oxides显示文摘 | GOVER R K B YONEMURA M HIRANO A | 1999 | Journal of Power Sources1999,,8182: | 2 |
| 8 | Stiffness of Rubber Bush Mountings Subjected to Radial Loading显示文摘 | HORTON J M Gover M J C TUPHOLME G E | 2000 | Rubber Chemistry and Technology2000,73,2: | 1 |
| 9 | 显示文摘 | Kanno R Mitchell B | 2000 | J Power Sources2000,90,: | 1 |
| 10 | Resistance of soils to concentrated flow erosion:A review显示文摘 | Knapen A Poesen J Govers G | 2007 | Earth-Science Reviews2007,80,: | 1 |
| 11 | Projected destination image online: Websitecontent analysis of pictures and text显示文摘 | Govers R Go F M | 2005 | Information Technology& Tourism2005,7,2: | 1 |
| 12 | RXLR effector reservoir in two phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members显示文摘 | Jiang R H Tripathy S Govers F | 2008 | Proceedings of the National Academy of Sciences of the United States of America2008,105,12: | 1 |
| 13 | Molecular mechanisms of GLUT4 regulation in adipocytes显示文摘 | R. Govers | 2014 | Diabetes and Metabolism2014,,: | 1 |
| 14 | A novel effect of an o- pioid receptor antagonist, naloxone, on the production of reactive oxygen species by microglia: a study by electron paramagnetic resonance spectroscopy显示文摘 | CHEN C J ROTA C GOVER RE | 2000 | Brain Res2000,854,: | 1 |
| 15 | Study of the preparationconditions for NiMn204 grown from hydroxide precursors 显示文摘 | ASHCROFT Q TERRY I GOVER R | 2006 | J EurCeram Soc2006,26,6: | 1 |
| 16 | Fetal omphalocele detected early in pregnancy:associated anomalies and outcomes显示文摘 | Blazer S Zimme EZ Gover A | | 0,,: | 1 |
| 17 | Deconstructing destination image in the information age显示文摘 | Govers R Go F M | | 0,,02: | 1 |
| 18 | Relationship between discharge, velocity and flow area for rills eroding loose, non-layeredmaterials显示文摘 | Govers G | 1992 | Earth Surface Proces- ses and Landforms1992,17,5: | 1 |
| 19 | Integrated Quality Management for Tourist Destinations : A European Perspective on Achieving Competitive- ness显示文摘 | Go FM Govers R | 2000 | Tourism Management2000,21,1: | 1 |
| 20 | Hybrid-ion a lithium-ion cell based on a sodium insertion material显示文摘 | Barker J Gover R K B Burns P | 2006 | Electrochemical and Solid-State Letters2006,9,4: | 1 |