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| 1 | 铁氨基黏土-葡萄糖氧化酶纳米复合催化剂的构筑及多酶级联反应研究显示文摘以铁氨基黏土(FeAC)为载体,通过共价交联固定葡萄糖氧化酶(GO_(x)),构筑了铁氨基黏土-葡萄糖氧化酶纳米复合催化剂(FeAC-GO_(x)).利用FeAC的过氧化物酶活性,与GO_(x)结合进行级联反应,可催化葡萄糖转化为过氧化氢并产生显色反应;采用扫描电子显微镜(SEM)、X射线衍射(XRD)和傅里叶变换红外光谱(FTIR)对FeACGO_(x)进行了形貌和结构表征,并评价了其酶动力学参数、催化稳定性和重复使用性等.结果表明,GO_(x)的固定化率可达到76.4%,所构筑的纳米结构酶复合体系具有高效的级联催化能力.与天然酶体系相比,FeAC-GO_(x)具有更优异的温度和pH耐受性,且在重复使用6次后,酶催化活性无明显降低.该体系不仅为新型葡萄糖传感器的开发奠定了基础,还为多酶级联纳米结构酶的构筑提供了新思路. | 李柳 孙仕勇 吕瑞 GOLUBEV Yevgeny Aleksandrovich 王可 董发勤 段涛 KOTOVA Olga Borisovna KOTOVA Elena Leonidovna | 2021 | 高等学校化学学报2021，42，3： | 2 |
| 2 | Homogeneous precipitation of magnesium phosphates from seawater solutions显示文摘 | Pokrovsky 0 S Savenko V S | 2001 | Crystal Growth2001，223，： | 1 |
| 3 | Plant rational transfer function approximation from input-output data显示文摘 | Horowitz I M | 1982 | International Journal of Control1982，36，4： | 1 |
| 4 | The generalized approximation method for modeling coagulation kinetics-part 1:justification and implementation of the method显示文摘 | Piskunov V N Golubev A I | 2002 | Journal of Aerosol Science2002，33，1： | 1 |
| 5 | Mycocin production in Cryptoccocus aquaticus显示文摘 | PFEIFFER I GOLUBEV W I FARLAS Z | 2004 | Antonie van Leeuwenhock2004，，86： | 1 |
| 6 | Evaporation losing its strength显示文摘 | Peterson T C Golubev V S Groisman 19 Y | 1995 | Nature1995，377，： | 1 |
| 7 | The generalized approximation method for modeling coagulation kinetics-part 2:comparison with other methods显示文摘 | Piskunov V N Golubev A I Barrett J C | 2002 | Journal of Aerosol Science2002，33，1： | 1 |
| 8 | Evaporation losing its strength显示文摘 | Peterson T C Golubev V S Groisman P Y | 1995 | Nature1995，337，： | 1 |
| 9 | Ewapomtion losing its strength 显示文摘 | Peterson Golubev Groisman | 1995 | Nature1995，，377： | 1 |
| 10 | Effect of intermolecular hydrogen bonding and proton transfer on fluorescence of salicylic acid 显示文摘 | Denisov G S Golubev N S Schreiber V M | 1997 | Journal of Molecular Structure1997，，： | 1 |
| 11 | Evaporation changes over the contiguous United States and the former USSR:A reassessment显示文摘 | Valentin S Golubev Jay H Lawrimore Pavel Ya Groisman | 2006 | Geophysical Research Letters2006，28，13： | 1 |
| 12 | Evaporation losing its strength显示文摘 | Peterson T C Golubev V S Groisman P Y | 1995 | Nature1995，377，： | 1 |
| 13 | Calcite,dolomite and magnesite dissolution kinetics in aqueous solutionsat acid to circumneutral pH,25 to 150°C and 1 to 55 atm pCO2:New constraintson CO2 sequestration in sedimentary basins显示文摘 | Pokrovsky Oleg S Golubev | 2009 | Chemical Geology2009，265，12： | 1 |
| 14 | Evaporation losing its strength显示文摘 | Peterson T C Golubev V S Groisman P Y | 1995 | Nature1995，337，： | 1 |
| 15 | Trimetazidine in Complex Therapy of Acute Myocardial Infarction at the Back-ground of Diabetes Mel itus Type 2显示文摘 | Tereshchenko S N Golubev A V Kositsyna I V | | 0，，01： | 1 |
| 16 | Evaporation losing it' s strength显示文摘 | Peterson T C Golubev V S Groisman P Y | 1995 | Nature1995，，377： | 1 |
| 17 | Calcite, dolomite and magnesite dissolution kinetics in aqueous solutions at acid to circumneutral pH, 25 to 150℃and 1 to 55 atm pCO2: New constraints on CO2 sequestration in sedimentary basins显示文摘 | POKROVSKY O S GOLUBEV S V SCHOTT J | 2009 | Chemical Geology2009，265，12： | 1 |
| 18 | Lipids of the fruit of Feijoa sellowiana显示文摘 | Kolesnik A A Golubev V N | 1991 | Chemistry of Natural Compounds1991，27，4： | 1 |
| 19 | Evaporation changes over the contiguous United States and the former USSR: A reassess ment显示文摘 | Golubev V S Lawrimore J H Groisman P Ya ct al | 2001 | Geophysical Research Letters2001，28，： | 1 |
| 20 | Correlation between surface properties and photocatalytie and photoelectrocbemical activity of In2O3 nanocrystalline films and powders 显示文摘 | Poznyak S K Golubev A N Kulak A I | 2000 | Surf Sci2000，，： | 1 |
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