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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Effect of climate change on greenhouse gas emissions from arable crop rotations显示文摘 | J?rgen E. Olesen Gitte H. Rub?k Tove Heidmann S?ren Hansen Christen D. B?rgensen | 2004 | Nutrient Cycling in Agroecosystems2004，，2： | 1 |
| 3 | Galectins:structure and function of a large family of animal lectins 显示文摘 | Barondes S H Cooper D N Gitt M A | 1994 | J Biol Chem1994，269，20： | 1 |
| 4 | Effects of a -lactalbumin-en-riched formula containing different concentrations of glycomacropep-tide on infant nutrition显示文摘 | OLOF S BO L GITTE G | 2008 | American Journal of Clinical Nutrition2008，87，4： | 1 |
| 5 | Galectins, structure and function of a large family of animal lectins显示文摘 | BARONDES S H COOPER D N GITT M A | 1994 | J Biol Chem1994，269，20： | 1 |
| 6 | Characterization of a non-fouling ultrafiltration membrane 显示文摘 | Jiang Wei Gitte S Helm Nick Comer-Walker | 2006 | Desalination2006，192，： | 1 |
| 7 | Moisture- induced Effects on Stacking Strength of Moulded - fibre Packaging in Varying Environmental Conditions 显示文摘 | SφRENSEN Gitte HOFFMANN John | 2004 | Packaging Technology and Science2004，17，5： | 1 |
| 8 | Glyco-conjugates as elicitors or suppressors of plant innate immunity 显示文摘 | Alba S Gitte E Tomonori S | 2010 | Glycobiology2010，20，4： | 1 |
| 9 | Blue-green algae as an immuno-enhancer and biomodulator显示文摘 | Gitte S Donald I Christian D | 2001 | Canada Winter2001，3，4： | 1 |
| 10 | Bacterial Resistance Levels in Danish Farmland as a Result of Treatment With Pig Manure Slurry显示文摘 | GITTE S YVONN A BENT H S | 2003 | Environment International2003，28，7： | 1 |
| 11 | Galectins: Structure and function of a large family of animal lectins 显示文摘 | Barondest S H Cooper D N Gitt M A | 1994 | J Biol Chem1994，269，20： | 1 |
| 12 | Blue-green algae as an immuno-enhancer and biomodulator 显示文摘 | Gitte S Donald I Christian D | 2001 | JANA2001，3，4： | 1 |
| 13 | 运动不仅是良医:上班族幸福感和高效工作的源泉(英文)显示文摘背景:体力活动包括运动中的肌肉活动、体力工作以及包含体育锻炼在内的闲暇活动。关于体力活动对健康的影响存在相互矛盾的研究结果,因为体力活动水平可能由于机械化的发展和竞技体育而下降,但如果依照国际运动推荐量进行运动可能会提高上班族幸福感及工作效率。方法:在丹麦已经进行15项随机对照试验,对不同工作场所中招募的3500余名员工进行运动锻炼实验。干预持续时间为10-52周,并根据'智能运动训练'(IPET)概念于工作时间提供每周1小时监督锻炼。IPET这一概念建立于体育科学训练原则证据基础上,并根据工作范围、员工健康状况和身体能力特别制定。在基线期和随访期进行问卷调查和体检(血液与肌肉取样)。受试者职业类型包括:办公室文员和计算机从业人员、牙科医生、工业技师、清洁工人、医护人员、建筑工人、战斗机,直升机飞行员。结果:所有研究组健康水平均有显著提高,具体表现为:办公室文员和计算机从业人员、牙科医生、工程师、医护人员、飞行员的颈部疼痛减少;办公室和计算机工作者、医护人员和建筑工人的心肺功能(心脏代谢疾病的健康风险指标)得到改善。此外还有其他方面的改善,如肌肉力量增加和平衡控制能力提高。更为重要的是,生产效率随肌肉力量改善和BMI下降而提高。结论:所有研究组健康状况均有改善,其效应值具有临床意义。因此,如果一项IPET方案能有专家指导以确保其效力,加上雇主支持,该项lPET便能提高健康水平。成本一效益估算表明,其开支节省金额相对于医疗费用及生产效率损失,IPET方案产生的成本是可接受的。 | Gisela Sj?gaard Jeanette Reffstrup Christensen Just Bendix Justesen Mike Murray Tina Dalager Gitte Hansen Fredslund Karen S?gaard | 2016 | Journal of Sport and Health Science2016，5，2： | 1 |
| 14 | Galectins: Structure and function of a large family of animal lectins 显示文摘 | Barondes S H Cooper D N Gitt M A | 1994 | J Biol Chem1994，269，20： | 1 |
| 15 | The arcuate nucleus mediates GLP-1 receptor agonist liraglutide-dependent weight loss显示文摘 | Secher Anna Jelsing Jacob Baquero Arian F Hecksher-S?rensen Jacob Cowley Michael A Dalb?ge Louise S Hansen Gitte Grove Kevin L Pyke Charles Raun Kirsten Sch?ffer Lauge Tang-Christensen Mads Verma Saurabh Witgen Brent M Vrang Niels Knudsen | 2014 | Journal of Clinical Investigation2014，，10： | 1 |
| 16 | Tandem repeat analysis for surveillance of human Salmonella Typhimurium infections 显示文摘 | Mia T Gitte S Bjom-Arne L | 2007 | Emerging infectious Diseases2007，13，3： | 1 |
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