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| 1 | A Roadmap toward Engineered Nitrogen-Fixing Nodule Symbiosis显示文摘In the late 19th century,it was discovered that legumes can establish a root nodule endosymbiosis with nitrogen-fixing rhizobia.Soon after,the question was raised whether it is possible to transfer this trait to non-leguminous crops.In the past century,an ever-increasing amount of knowledge provided unique insights into the cellular,molecular,and genetic processes controlling this endosymbiosis.In addition,recent phylogenomic studies uncovered several genes that evolved to function specifically to control nodule formation and bacterial infection.However,despite this massive body of knowledge,the long-standing objective to engineer the nitrogen-fixing nodulation trait on nonleguminous crop plants has not been achieved yet.In this review,the unsolved questions and engineering strategies toward nitrogen-fixing nodulation in non-legume plants are discussed and highlighted. | Rik Huisman Rene Geurts | 2020 | Plant Communications2020，1，1： | 4 |
| 2 | Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain显示文摘AIM: To establish a novel, sensitive and high-throughput gelatinolytic assay to define new inhibitors and compare domain deletion mutants of gelatinase B/matrix metalloproteinase (MMP)-9. METHODS: Fluorogenic Dye-quenched (DQ)TM-gelatin was used as a substrate and biochemical parameters (substrate and enzyme concentrations, DMSO solvent concentrations) were optimized to establish a highthroughput assay system. Various small-sized libraries (ChemDiv, InterBioScreen and ChemBridge) of hetero-cyclic, drug-like substances were tested and compared with prototypic inhibitors. RESULTS: First, we designed a test system with gelatin as a natural substrate. Second, the assay was validated by selecting a novel pyrimidine-2,4,6-trione (barbitu- rate) inhibitor. Third, and in line with present structural data on collagenolysis, it was found that deletion of the O-glycosylated region significantly decreased gelatinolytic activity (kcat/kM ± 40% less than full-length MMP-9). CONCLUSION: The DQTM-gelatin assay is useful in high-throughput drug screening and exosite targeting. We demonstrate that flexibility between the catalytic and hemopexin domain is functionally critical for gelatinolysis. | Jennifer Vandooren Nathalie Geurts Erik Martens Philippe E Van den Steen Steven De Jonghe Piet Herdewijn Ghislain Opdenakker | 2011 | World Journal of Biological Chemistry2011，2，1： | 3 |
| 3 | Rhizobium Lipo-chitooligosaccharide Signaling Triggers Accumulation of Cytokinins in Medicago truncatula Roots显示文摘 | Arjan van Zeijl Rik H.M. Op den Camp Eva E. Deinum Tatsiana Charnikhova Henk Franssen Huub J.M. Op den Camp Harro Bouwmeester Wouter Kohlen Ton Bisseling Rene Geurts | 2015 | Molecular Plant2015，8，8： | 3 |
| 4 | Trends in treatment and overall survival among patients with proximal esophageal cancer显示文摘BACKGROUND The management of proximal esophageal cancer differs from that of tumors located in the mid and lower part of the esophagus due to the close vicinity of vital structures.Non-surgical treatment options like radiotherapy and definitive chemoradiation(CRT)have been implemented.The trends in(non-)surgical treatment and its impact on overall survival(OS)in patients with proximal esophageal cancer are unclear,related to its rare disease status.To optimize treatment strategies and counseling of patients with proximal esophageal cancer,it is therefore essential to gain more insight through real-life studies.AIM To establish trends in treatment and OS in patients with proximal esophageal cancer.METHODS In this population-based study,patients with proximal esophageal cancer diagnosed between 1989 and 2014 were identified in the Netherlands Cancer Registry.The proximal esophagus consists of the cervical esophagus and the upper thoracic section,extending to 24 cm from the incisors.Trends in radiotherapy,chemotherapy,and surgery,and OS were assessed.Analyses were stratified by presence of distant metastasis.Multivariable Cox proportional hazards regression analyses was performed to assess the effect of period of diagnosis on OS,adjusted for patient,tumor,and treatment characteristics.RESULTS In total,2783 patients were included.Over the study period,the use of radiotherapy,resection,and CRT in non-metastatic disease changed from 53%,23%,and 1%in 1989-1994 to 21%,9%,and 49%in 2010-2014,respectively.In metastatic disease,the use of chemotherapy and radiotherapy increased over time.Median OS of the total population increased from 7.3 mo[95%confidence interval(CI):6.4-8.1]in 1989-1994 to 9.5 mo(95%CI:8.1-10.8)in 2010-2014(logrank P<0.001).In non-metastatic disease,5-year OS rates improved from 5%(95%CI:3%-7%)in 1989-1994 to 13%(95%CI:9%-17%)in 2010-2014(logrank P<0.001).Multivariable regression analysis demonstrated a significant treatment effect over time on survival.In metastatic disease,median OS was 3.8 mo(95%CI:2.5-5.1)in 1989-1994,and 5.1 mo(95%CI:4.3-5.9)in 2010-2014(logrank P=0.26).CONCLUSION OS significantly improved in non-metastatic proximal esophageal cancer,likely to be associated with an increased use of CRT.Patterns in metastatic disease did not change significantly over time. | Judith de Vos-Geelen Sandra ME Geurts Liselot BJ Valkenburg-van Iersel Evelien JM de Jong Vivianne CG Tjan-Heijnen Margreet van Putten Valery EPP Lemmens Heike I Grabsch Nadia Haj Mohammad Frank JP Hoebers Chantal V Hoge Paul M Jeene Hanneke WM van Laarhoven Tom Rozema Marije Slingerland Grard AP Nieuwenhuijzen | 2019 | World Journal of Gastroenterology2019，25，47： | 2 |
| 5 | 光照及其对猪福利和生产的影响显示文摘虽然经常被人们忽视,但光照肯定是养猪生产管理中的一个重要组成部分。它会直接影响猪的福利和生产性能。
重要的是要根据猪的视觉和需求调整光照。光照会影响猪的生物钟、生物节律、社会行为和整体活动。正确的光照取决于几个因素:光谱、分布、(非)闪光、光照强度和光照周期(后两者是最重要的因素)。 | 王晶晶(译) 张配配(审) 靳文广(制图表) Kim Geurts | 2018 | 国外畜牧学(猪与禽)2018，38，8： | 2 |
| 6 | 光照及其对肉种鸡福利和生产性能的影响显示文摘人们越来越认识到向肉种鸡提供良好光照的重要性,在我们看来,这是有正常理由的。理想的光照条件会对肉种鸡的福利和生产性能产生重要的影响。 | 唐彩琰 张配配 何闪 Kim Geurts | 2019 | 国外畜牧学(猪与禽)2019，39，8： | 2 |
| 7 | Transcriptional Regulation of Nutrient Exchange in Arbuscular Mycorrhizal Symbiosis显示文摘Most land plants rely on the symbiosis with arbuscular mycor- rhizal (AM)fungi to acquire sufficient mineral nutrients from soils. Plants host AM fungi inside root cortex cells,where the fungus forms highly branched structures called arbuscules.Arbuscules are surrounded by a specialized host membrane across which nutrients are exchanged in a cooperative manner.In return for mineral nutrients,the fungus obtains sugars and fatty acids from the plant (Luginbuehl and Oldroyd,2017). | Erik Limpens Rene Geurts | 2018 | Molecular Plant2018，11，12： | 2 |
| 8 | Biomarker discovery for inflammatory bowel disease, using proteomic serum profiling显示文摘 | Marie-Alice Meuwis Marianne Fillet Pierre Geurts Dominique de Seny Laurence Lutteri Jean-Paul Chapelle Vincent Bours Louis Wehenkel Jacques Belaiche Michel Malaise Edouard Louis Marie-Paule Merville | 2007 | Biochemical Pharmacology2007，，9： | 2 |
| 9 | Altered expression patterns of group Ⅰ and Ⅱ metabotropic glutamate receptors in multiple sclerosis显示文摘 | Geurts JJ Wolswijk G Bo L | 2003 | Brain2003，126，8： | 1 |
| 10 | A home-based,self-administered stimulation program to improve selected hand functions of chronic stroke 显示文摘 | Alon G Sunnerhagen KS Geurts AC | 2003 | NeuroRehabilitation2003，18，21： | 1 |
| 11 | Knockout rats via embryo microinjection of zlnc-finger nucleases 显示文摘 | Geurts AM Cost GJ Freyvert Y | 2009 | Science2009，325，5939： | 1 |
| 12 | Glucose metabolism: Fo- cus on gut microbiota, the endocannabinoid system and beyond 显示文摘 | Cani PD Geurts L Matamoros S | 2014 | Diabetes Metab2014，40，4： | 1 |
| 13 | Masked t(X;18) (p11;q11) in a biphasic synovial sarcoma revealed by FISH and RT-PCR 显示文摘 | Geurts van Kessel A de Bruijn D Hermsen L | 1998 | Genes Chromosomes Cancer1998，23，2： | 1 |
| 14 | How Specific is a Deficit of Executive Functioning for Attention -Deficit / Hyperactivity Disorder显示文摘 | Geurts H Oosterlaan J | 2002 | Behav Brain Res2002，，130： | 1 |
| 15 | Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon显示文摘 | Geurts A M Wilber A Carlson C M | 2006 | BMC Biotechnol2006，6，： | 1 |
| 16 | Large-eddy simulation of the turbulent mixing layer显示文摘 | Vreman B Geurts B Kuerten H | 1997 | J Fluid Mech1997，339，： | 1 |
| 17 | Grey matter pathology in multiple sclerosis显示文摘 | Geurts JJ Barkhof F | | 0，，9： | 1 |
| 18 | Chronic endocanna- binoid system stimulation induces muscle macrophage and lipid accumulation in type 2 diabetic mice independently of metabol- ic endotoxaemia显示文摘 | Geurts L Muceioli GG Delzenne NM | 2013 | PLoS One2013，8，55： | 1 |
| 19 | Realizability conditions for the turbulent stress tensor in large eddy simulation显示文摘 | Vreman B Geurts B Kuerten H | 1994 | Journal of Fluid Mechanics1994，278，： | 1 |
| 20 | Motor recovery after stroke:a systematic review of the literature显示文摘 | Hendricks HT Van Limbeek J Geurts AC | 2002 | Arch Phys Med Rehabil2002，83，11： | 1 |
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