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| 1 | Targeted mutagenesis in rice using CRISPR-Cas system显示文摘 | Jin Miao Dongshu Guo Jinzhe Zhang Qingpei Huang Genji Qin Xin Zhang Jianmin Wan Hongya Gu Li-Jia Qu | 2013 | Cell Research2013，23，10： | 103 |
| 2 | Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis显示文摘类胡萝卜素在植物和 phytoene desaturase 基因(PDS3 ) 在许多生理的过程起一个重要作用把重要的酶之一编码为类胡萝卜素生合成小径。这里,我们报导 T-DNA 插入异种 ofPDS3 基因的鉴定和分析。功能的互补证实 thepds3 异种的白化体和矮子显型源于 PDS3 基因的功能的混乱。叶绿体开发在 pds3 异种在专业版质体阶段被逮捕。进一步的分析证明高级 ofphytoene 在 pds3 异种被积累。外长的 GA (3 ) 的增加能部分救矮子显型,建议 pds3 异种的矮子显型可能由于 GAdeficiency。Microarray 和 RT-PCR 分析证明那破坏 PDS3 基因导致了涉及至少 20 条新陈代谢的小径的基因表示变化,包括在类胡萝卜素,叶绿素,和 GA 的许多基因的抑制生合成小径。我们的数据建议在 pds3 异种的积累的 phytoene 可能在某些否定反馈起一个重要作用影响多样的细胞的小径的基因表示。 | Genji Qin Hongya Gu Ligeng Ma Yiben Peng Xing Wang Deng Zhangliang Chen Li-Jia Qu | 2007 | Cell Research2007，17，5： | 39 |
| 3 | Over-expression of WOX1 Leads to Defects in Meristem Development and Polyamine Homeostasis in Arabidopsis显示文摘In plants,the meristem has to maintain a separate population of pluripotent cells that serve two main tasks,i.e.,self-maintenance and organ initiation,which are separated spatially in meristem.Prior to our study,WUS and WUS-like WOX genes had been reported as essential for the development of the SAM.In this study,the consequences of gain of WOX1 function are described.Here we report the identification of an Arabidopsis gain-of-function mutant wox1-D,in which the expression level of the WOX1(WUSCHEL HOMEOBOX 1) was elevated and subtle defects in meristem development were observed.The wox1-D mutant phenotype is dwarfed and slightly bushy,with a smaller shoot apex.The wox1-D mutant also produced small and dark green leaves,and exhibited a failure in anther dehiscence and male sterility.Molecular evidences showed that the transcription of the stem cell marker gene CLV3 was down-regulated in the meristem of wox1-D but accumulated in the other regions,i.e.,in the root-hypocotyl junction and at the sites for lateral root initiation.The fact that the organ size and cell size in leaves of wox1-D are smaller than those in wild type suggests that cell expansion is possibly affected in order to have partially retarded the development of lateral organs,possibly through alteration of CLV3 expression pattern in the meristem.An S-adenosylmethionine decarboxylase(SAMDC) protein,SAMDC1,was found able to interact with WOX1 by yeast two-hybrid and pull-down assays in vitro.HPLC analysis revealed a significant reduction of polyamine content in wox1-D.Our results suggest that WOX1 plays an important role in meristem development in Arabidopsis,possibly via regulation of SAMDC activity and polyamine homeostasis,and/or by regulating CLV3 expression. | Yanxia Zhang Renhong Wu Genji Qin Zhangliang Chen Hongya Gu Li-Jia Qu | 2011 | Journal of Integrative Plant Biology2011，53，6： | 23 |
| 4 | Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development显示文摘兼容丑恶纸巾的表面上的花粉萌芽是为植物授精的必要的步。这里,我们报导 Arabidopsis 变异的 bcl1 是由于花粉萌芽的失败不育的男性。我们证明 bcl1 异种等位基因不能被男配偶体播送,没有同型结合的 bcl1 异种被获得。花粉发育阶段的分析显示 bcl1 变化影响花粉萌芽然而并非花粉成熟。分子的分析证明花粉萌芽的失败被 AtBECLIN 1 的混乱引起。AtBECLIN 1 在成熟花粉主要被表示并且与重要相同编码蛋白质到在酵母为 autophagyand vacuolar 蛋白质排序的过程(VPS ) 要求的 Beclin1/Atg6/Vps30。我们也证明 AtBECLIN 1 为正常被要求植物开发,和那与 autophagy, VPS 和 glycosylphosphatidylinositolanchor 系统有关的基因,被 AtBECLIN 1 的缺乏影响。 | Genji Qin Zhiqiang Ma Li Zhang Shufan Xing Xianhui Hou Jie Deng Jingjing Liu Zhangliang Chen Li-Jia Qu Hongya Gu | 2007 | Cell Research2007，17，3： | 17 |
| 5 | Disruption of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) gene results in albino, dwarf and defects in trichome initiation and stomata closure in Arabidopsis显示文摘1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR ) 是涉及 2-C-methyl-D-erythritol-4-phosphate (MEP ) 的重要的酶为 isoprenoid 生合成提供基本五碳的单位的小径。在植物开发和新陈代谢调查 MEP 小径的角色,我们在 dxr 异种(GK_215C01 ) 和二根 DXR 转基因的合作抑制线上执行了详细分析, OX-DXR-L2 和 OX-DXR-L7。我们发现 dxr 异种是白化体和矮子。它从来没跑,显著地减少了毛状体的数字,大多数 stomata 不能在叶子通常关门。二根合作抑制线没有毛状体生产了更黄的开花期和白化体萼片。涉及毛状体开始的基因的抄写层次被发现强烈被假装,包括 GLABRA1,透明种皮光洁 1, TRIPTYCHON 并且细长纤弱,哪个被 gibberellic 酸(气体) 调整的表示。而 abscisic 酸(骆驼毛的织物) 的外长的申请能救 stomata 闭合缺点, GA3 的外长的申请能部分救矮子显型和 dxr 的毛状体开始,建议 GA 和骆驼毛的织物的底层贡献在 dxr 异种的显型。我们进一步发现涉及 GA 和骆驼毛的织物的 biosynthetic 小径的基因并列地被调整。这些结果显示 plastidial MEP 小径的那混乱导致光合的颜料,气体和骆驼毛的织物的 biosynthetic 缺乏,并且这样发展畸形,并且从细胞质的 mevalonate 小径的流动不是足够的救缺乏,这由 plastidial MEP 小径的阻塞引起了。这些结果在控制 isoprenoids 的生合成为 MEP biosynthetic 小径揭示一个关键角色。 | Shufan Xing Jin Miao Shuang Li Genji Qin Si Tang Haoni Li Hongya Gu Li-Jia Qu | 2010 | Cell Research2010，20，6： | 17 |
| 6 | The molecular mechanism of SPOROCYTELESS/NOZZLE in controlling Arabidopsis ovule development显示文摘小卵为植物复制是必要的并且在授精以后发展成种子。当为在 Arabidopsis 的小卵开发的一个必要因素,而是系统程序设计语言功能的生物化学的性质仍然保持未解决, SPOROCYTELESS/NOZZLE (SPL/NZZ ) 以超过 15 年著名。这里,我们证明系统程序设计语言作为一个像适配器的 transcriptional 抑压者工作。我们证明禁止 CINCINNATA (CIN ) 的系统程序设计语言成员 TOPLESS/TOPLESS-RELATED (TPL/TPR ) 合作抑压者喜欢 TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP ) 抄写因素。我们表明系统程序设计语言在 C 终端结束使用它的耳朵主题到成员 TPL/TPRs 和它的 N 终端部分绑并且禁止 TCP。我们部分表明 TPL/TPRs 的那任何一个混乱或 TCP 的 overexpression phenocopies 在系统程序设计语言的 megasporogenesis 的缺点。而且, TCP 的混乱引起类似于 spl-D gain-of-function 异种的显型。这些结果为系统程序设计语言定义行动机制,它由镇压与 TPL/TPRs 一起控制小卵开发关键抄写因素的活动。我们的调查结果建议类似的基因压抑策略被植物和真菌采用到控制孢子。 | Baoye Wei Jinzhe Zhang Changxu Pang Hao Yu Dongshu Guo Hao Jiang Mingxin Ding Zhuoyao Chen Qing Tao Hongya Gu Li-Jia Qu Genji Qin | 2015 | Cell Research2015，25，1： | 9 |
| 7 | Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34显示文摘VPS15 蛋白质是在酵母和哺乳动物的房间的发展起一个枢轴的作用的 phosphatidylinositol 3-kinase 建筑群的一个部件。它在植物的相当或相同事物的功能的知识仍然保持有限。这里,我们报导那 AtVPS15,在 Arabidopsis 的酵母 VPS15p 的一个相当或相同事物,在花粉萌芽起一个必要作用。AtVPS15 的同型结合的 T-DNA 插入异种不能从 self-pollinated 的子孙被获得异质接合的异种。在 atvps15 异种和野类型的 Arabidopsis 之间的相互的十字表明 T-DNA 插入不能被男配偶体播送。显微镜的分析显示出的染色的 DAPI,亚历山大的污点和扫描电子那 atvps15 异质接合的植物生产了与野类型的花粉词法上难区分的花粉谷物,而 in vitro 萌芽实验揭示了那,花粉谷物的萌芽是有缺点的。染色表示 AtVPS15 倡导者驾驶的 GUS 记者基因的转基因的植物的分析的 GUS 显示出那 AtVPS15 主要在花粉谷物被表示。最后,二混血儿的分析表明了的 DUALmembrane 酵母那 AtVPS15 可能与 AtVPS34 直接交往。这些结果建议 AtVPS15 为花粉萌芽是很重要的,可能通过 PI3-kinase 的活动的调整。 | Wei-Ying Wang Li Zhang Shufan Xing Zhiqiang Ma Jingjing Liu Hongya Gu Genji Qin Li-Jia Qu | 2012 | Journal of Genetics and Genomics2012，39，2： | 6 |
| 8 | The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of Micro RNA156f显示文摘Plant architecture is an important factor for crop production. Some members of micro RNA156(mi R156) and their target genes SQUAMOSA Promoter-Binding Protein-Like(SPL) were identi fi ed to play essential roles in the establishment of plant architecture. However, the roles and regulation of mi R156 is not well understood yet. Here, we identi fi ed a T-DNA insertion mutant Osmtd1(Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and Osmi R156 f was signi fi cantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of Osmi R156 f in wild-type rice phenocopied Osmtd1. We showed that the expression of Os SPL3, Os SPL12,and Os SPL14 were signi fi cantly downregulated in Osmtd1 or Osmi R156 f overexpressed lines, indicating that Os SPL3,Os SPL12, and Os SPL14 were possibly direct target genes of Osmi R156 f. Our results suggested that Osmi R156 f controlledplant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein. | Qing Liu Gezhi Shen Keqin Peng Zhigang Huang Jianhua Tong Mohammed Humayun Kabir Jianhui Wang Jingzhe Zhang Genji Qin Langtao Xiao | 2015 | Journal of Integrative Plant Biology2015，57，10： | 5 |
| 9 | GAMT2 Encodes a Methyltransferase of Gibberellic Acid That is Involved in Seed Maturation and Germination in Arabidopsis显示文摘水杨酸甲基转移酶,苯甲酸甲基转移酶 andtheobromine 甲基转移酶家庭蛋白质属于甲基转移酶的一个唯一的班罐头甲醇化物小分子的混合物包括 IAA,水杨酸(SA ) 和 jasmonic 酸,在植物。我们报导 GAMT2 蛋白质,在氨基酸顺序与 IAMT1 有 34.2% 类似,装甲醇化物 gibberellic 酸(GA ) 。生物信息学分析建议那 GAMT2 也许能比 SA 大的甲醇化物一分子。GAMT2 主要在 A 在发展中的种子胚胎和内乳被表示。thaliana。在种子萌芽期间, GAMT2 的表示减少了直到子叶从种皮膨胀了。过去在 A 的 GAMT2 的表示。在多重显型,包括的侏儒症,延迟的生长,迟了的开花和减少的生育力的 thalianaresulted,它类似于 GA 缺乏的异种的显型。而 GAMT2 空异种(SALK_075450 ) 对如此的处理稍微感觉迟钝,种子萌芽试金证明在在植物的表示上的 GAMT2 对 GA 生合成禁止者(ancymidol ) 和 abscisic 酸(骆驼毛的织物) 处理过分敏感,建议那 GAMT2 可以甲醇化物 GA 或骆驼毛的织物。Enzymeactivity 分析显示 GAMT2 对进 methyl-GA3 试管内的甲醇化物 GA3 有能力,但是不能甲醇化物骆驼毛的织物。在表示植物上的 GAMT2 上的 Microarray 分析也许建议了那 methyl-GA 在 A 的 GA 的一种不活跃的形式。thaliana。这些数据建议 GAMT2 被 modulating GA 活动涉及种子成熟和萌芽…… | Shufan Xing Genji Qin Yan Shi Zhangliang Chen Hongya GU Li-Jia Qu | 2007 | Journal of Integrative Plant Biology2007，49，3： | 4 |
| 10 | Arabidopsis transcription factor TCP4 represses chlorophyll biosynthesis to prevent petal greening显示文摘Green petals pose a challenge for pollinators to distinguish flowers from leaves,but they are valuable as a specialty flower trait.However,little is understood about the molecular mechanisms that underlie the development of green petals.Here,we report that CINCINNATA(CIN)-like TEOSINTE BRANCHED 1/CYCLOIDEA/PCF(TCP)proteins play key roles in the control of petal color.The septuple tcp2/3/4/5/10/13/17 mutant produced flowers with green petals due to chlorophyll accumulation.Expression of TCP4 complemented the petal phenotype of tcp2/3/4/5/10/13/17.We found that chloroplasts were converted into leucoplasts in the distal parts of wild-type petals but not in the proximal parts during flower development,whereas plastid conversion was compromised in the distal parts of tcp2/3/4/5/10/13/17 petals.TCP4 and most CIN-like TCPs were predominantly expressed in distal petal regions,consistent with the green–white pattern in wild-type petals and the petal greening observed in the distal parts of tcp2/3/4/5/10/13/17 petals.RNA-sequencing data revealed that most chlorophyll biosynthesis genes were downregulated in the white distal parts of wild-type petals,but these genes had elevated expression in the distal green parts of tcp2/3/4/5/10/13/17 petals and the green proximal parts of wild-type petals.We revealed that TCP4 repressed chlorophyll biosynthesis by directly binding to the promoters of PROTOCHLOROPHYLLIDE REDUCTASE(PORB),DIVINYL REDUCTASE(DVR),and SUPPRESSOR OF OVEREXPRESSION OF CO 1(SOC1),which are known to promote petal greening.We found that the conversion of chloroplasts to leucoplasts and the green coloration in the proximal parts of petals appeared to be conserved among plant species.Our findings uncover a major molecular mechanism that underpins the formation of petal color patterns and provide a foundation for the breeding of plants with green flowers. | Xinhui Zheng Jingqiu Lan Hao Yu Jingzhe Zhang Yi Zhang Yongmei Qin Xiao-Dong Su Genji Qin | 2022 | Plant Communications2022，3，4： | 4 |
| 11 | A hybrid particle swarm optimization for distribution state estimation显示文摘 | Shigenori Naka Takamu Genji | 2003 | IEEE Trans on Power Systems2003，18，1： | 2 |
| 12 | Inclusion control of automotive spring steel in the basic oxygen furnace and ingot casting process显示文摘The process of automotive spring steel in the basic oxygen furnace and ingot casting(BOF-IC)of Baosteel is described in this paper.High quality spring steel can be obtained through designing reasonable deoxidization and alloying processes,enhancing steel purity and reducing inclusions in steel. | ZHANG Jianchun DI Yongzhong HU Huijun WAN Genjie | 2010 | Baosteel Technical Research2010，4，2： | 2 |
| 13 | A Hybrid Particle Swarm Optization for Distribution State Estimation显示文摘 | Naka S Genji T Yura T | 2003 | IEEE Transaction Power System2003，18，1： | 1 |
| 14 | 400 V class high-speed current limiting circuit breaker for electric power system显示文摘 | GENJI T NAKAMURA O ISOZAKI M | 1994 | IEEE Transactions on Power Delivery1994，9，3： | 1 |
| 15 | Hybrid genetic algorithm with fuzzy logic for resource - constrained project scheduling 显示文摘 | Kwan W K Mitsuo G Genji Y | 2003 | Applied soft computing2003，2，3： | 1 |
| 16 | Com-parative Study of Modern Heuristic Algorithms to Service Restoration in Distribution Systems 显示文摘 | Toune S Fudo H Genji T | 2002 | IEEE Trans on Power Delivery2002，17，1： | 1 |
| 17 | D-myo-inositol-3-phosphate affects phosphatidylinositol-mediated endomembrane function in Arabidopsis and is essential for auxin-regulated embryogenesis显示文摘 | LUO Yu QIN Genji ZHANG Jun | 2011 | Plant Cell2011，23，4： | 1 |
| 18 | Practical Distribution State Estimation Using Hybrid Particle Swarm Optimization显示文摘 | Naka S Genji T Yura T | | 0，，： | 1 |
| 19 | 400V class high-speed current limiting circuit breaker for electric power system显示文摘 | Genji T Nakamura O Isozaki M | 1994 | IEEE Transactions on Power Delivery1994，9，3： | 1 |
| 20 | 400 V class high-speed current limiting circuit breaker for electric power system显示文摘 | GENJI T NAKAMURA O ISOZAKI M | 1994 | IEEE Transactions on Power Delivery1994，9，： | 1 |
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