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| 1 | Differentiation of Embryonic Stem Cells into Neurons and Retina-like Structure in Nude Mice显示文摘Purpose:To investigate the intraocular growth and biological characteristics of mice embryonic stem cells in nude mice.Methods:Murine embryonic stem cells(D3 cell line)were cultured and maintained in an undifferentiated state in vitro,then transplanted into the anterior chamber of nude mice.Mophological and immunohistochemical examinations were implemented.Results:Two to three days after transplantation,yellow-white floating granules,sheets and masses were seen inside the anterior chamber and vitreous cavity,and enlarged gradually,14-20days later,the mice were executed.Morphological examination showed that there were undifferentiated cells and some round or polygonal differentiated cells in anterior chamber and vitreous cavity.The morphology of these differentiated cells were similar to that of the retina.The cells were highly positive in NSE staining.Conclusion:The tranplanted embryonic stem cells cold grow in the eyes of nude mice with tendency to differentiate into neurons and retina-like structure. | LiYP GeJ | 1999 | 眼科学报1999，15，3： | 7 |
| 2 | Corrective Change of Retinal Thickness Measured by Optical Coherence Tomography and Histologic Studies显示文摘Purpose:To evaluate the correlation of retinal thickness between optical coherence tomography(OCT)images and histologic slides.Methods:Retinal thickness was measured in 16 rabbit retinal histologic slides.The same eyes has been previously measured by OCT fr the comparison of results between two methods.Retinal thickness of each OCT image section was measured using both the manually assisted(requiring localization of reflectivity peaks by observer)and automated modes of the computer software.Results:Retinal thickness measured by OCT demonstrated a high degree of correlation with retinal histologic study.The automated method(Cc=0.66,P<0.01) was less reliable than the manually assisted one (Cc=0.84,P<0.001).The former had an error in 95% confidence interval,ranged between-0.71 and 11.09μm.The latter had a less error,ranged from -2.99 to 5.13μm.Conclusion:OCT can quantitatively measure the retinal thickness.However,automatical identification of the reflective boundaries by computer may result in errors in some cases.To masure the retinal thickess by manually assisted mode can increase the accuracy. | GeJ LuoRJ | 1999 | 眼科学报1999，15，3： | 3 |
| 3 | The Study of Influencing Factors on the Growth Characters of Sprague-Dawley Rat Retinal Neurons in Vitro显示文摘Purpose:To investigate the influencing factors in culturing Srague-Dawley(S-D) rats retinal neurons in order to lay foundation for further experimental research.Materials and Methods:Retinal cells were plated on plastic plates and coverslips coated with poly-lysine or ethylene imine polymer for primary culture.The cultured cells were divided into following groups:1.Culture medium changed every 2 tp 3 days vs changed only once;2.Cytosine arabinoside(Ara-C)added to the culture medium vs not added.The cells were observed and pictured under inverted phase contrast microscope.The cells were identified through immunocytochemistry.Results:The immunofluorescence showed that most of the cultured cells were neurons,among them were a few retinal ganglion cells.In the cultured group of which substrata coated with poly-l-lysine and culture medium added with Ara-c,the neurons intended to aggregate into clusters with relatively straight neurites.In the group of which substrata coated with ethylene imine polymer and medium added with Ara-c,the neurons grew dispersively with bent neurites.Both of them survived for 2 to 3 weeks.The cells which plated in the medium not added with Ara-c did not aggregate into clusters and survived longer than 4 weeks.In the group of which medium changed several times,the survival time of neurons was shorter than that in the medium changed only once.Conclusions:The retinal neurons plated on the substrata coated with ethylene imine polymer are easy to observe because of its dispersive growth.It is not favorable for the growth of the neurons by changing culture medium many times.Ara-c may possibly have side effect on the growth of retinal neurons. | LiuHQ GeJ | 1999 | 眼科学报1999，15，3： | 2 |
| 4 | Agerelated changes in collagen properties~and mineralization in cancellous and cortical bone in the porcine mandibular condyle显示文摘 | Willems NMBK Langenbach GEJ Everts V | 2010 | Calcif Tissue Int2010，86，4： | 1 |
| 5 | 显示文摘 | GeJ P Li Y D | 2003 | Chem Commun2003，19，： | 1 |
| 6 | Mass properties of the pig mandible显示文摘 | Zhang F Langenbach GEJ Hannam AG | 2001 | J Dental Res2001，80，1： | 1 |
| 7 | Subunit composition, regulatory properties, and phosphorylation of phosphofructokinase from human gliomas 显示文摘 | Staal GEJ Kalff A Heesbeen EC | 1987 | Cancer Res1987，47，19： | 1 |
| 8 | Resveratrol protects human lens epithelial cells against H202-induced oxidative stress by increasing catalase, SOD-l, and HO- 1 expression显示文摘 | Zheng Y Liu Y GeJ | 2010 | Mol Vis2010，16，： | 1 |
| 9 | Comparison of intravascular ultrasound ,and angiography in the assessment of myocardial bridging显示文摘 | GeJ ErbelR RupprechtHJ | 1994 | Circulation1994，89，： | 1 |
| 10 | A validation study of the Qiagen Investigator DIPplex (R) kit; an INDEL?based assay for human identification显示文摘 | Larue BL GeJ KingJL | 2012 | IntJ Legal Med2012，126，4： | 1 |
| 11 | Prevalence of cardiovascu?lar disease risk factor in the Chinese population: the 2007- 2008 China National Diabetes and Metabolic Disorders Study显示文摘 | Yang ZJ LiuJ GeJ P | 2012 | Eur HeartJ2012，33，2： | 1 |
| 12 | Preloading with atorvastatin before percutaneous coronary intervention in statin-na?ve Asian patients with non-ST elevation acute coronary syndromes: a randomized study显示文摘 | JangY ZhuJ GeJ | 2014 | J Cardiol2014，63，33： | 1 |
| 13 | Superparamagnetic magnetite colloidal nanocrystal clusters显示文摘 | GEJ HU Y BIASINI M | 2007 | Angew Chem Int Ed2007，46，： | 1 |
| 14 | Controllable synthesis of Pd nanocatalysts for direct Iormic acid fuel cell (DFAFC) application: From pd hollow nanospheres to Pd nanoparticles 显示文摘 | GeJ Xing W Xue X | 2007 | Journal of Physical Chemistry C2007，111，17： | 1 |
| 15 | Insemination of heifers with sexed sperm显示文摘 | Seidel GEJ Schenk JR Herlckhoff LA Doyle SP Brink Z Green RD Cran DG | | Theriogenology0，52，8： | 1 |
| 16 | Retinoid receptors in gastric cancer: expression and influence on prognosis显示文摘 | HU K W CHEN F H GEJ F | 2012 | Asian Pac} Cancer Pre v2012，13，5： | 1 |
| 17 | Implication of Th17 and Th1 cells in patients with chronic active hepatitis B显示文摘 | GeJ Wang K Meng QH | 2010 | J Clin Immunol2010，300，： | 1 |
| 18 | Stromal-derived factor-I deficiency in the bone marrow of aute myeloid leukemia显示文摘 | GeJ Hou R Liu Q | 2011 | IntJ Hematol2011，93，6： | 1 |
| 19 | Screening of ruptured plaques in patients with coronary artery disease by intravascular ultrasound显示文摘 | GeJ Chirillo F Schwedtmann J | | 0，，： | 1 |
| 20 | ExpressionofLysine-specificdemeth-ylase1inhumanepithelialovariancancer显示文摘 | ChenC GeJ LuQ etal | 2015 | JOvarianRes2015，8，1： | 1 |
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