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1PRGL:A cell wall proline-rich protein containning GASA domain in Gerbera hybrida显示文摘PRPs (proline-rich proteins) are a group of cell wall proteins characterized by their proline and hy- droproline-rich repetitive peptides. The expression of PRPs in plants is stimulated by wounding and environmental stress. GASA (gibberellic acid stimulated in Arabidopsis) proteins are small peptides sharing a 60 amino acid conserved C-terminal domain containing twelve invariant cysteine residues. Most of GASAs reported are localized to apoplasm or cell wall and their expression was regulated by gibberellins (GAs). It has been reported that, in French bean, these two proteins encoding by two distinct genes formed a two-component chitin-receptor involved in plant-pathogen interactions when plant was infected. We cloned a full-length cDNA of PRGL (proline-rich GASA-like) gene which encodes a protein containing both PRP and GASA-like domains. It is demonstrated that PRGL is a new protein with characteristics of PRP and GASA by analyzing its protein structure and gene expression.PENG JianZong, LAI LiuJing & WANG XiaoJing College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China 2008Science China(Life Sciences)2008,51,6:9
2Inducible expression pattern of rice Bowman-Birk inhibitorgene OsWIP1-2 and its protease inhibitory activity显示文摘The WIP1-2 gene was cloned from rice. It be-longs to the Bowman-Birk inhibitor gene family. Northern blot showed that expression of this gene was induced by wounding and jasmonic acid (JA). It indicates that the OsWIP1 gene plays an important role in the rice defense sys-tem. The OsWIP1-2 was cloned into pET28a and expressed in E. coli. Its expressed product was purified in the form of fusion protein and tested for the inhibitory activities against trypsin and chymotrypsin. It was found that the fusion pro-tein could inhibit chymotrypsin, but not trypsin. It was also found that the His tag at its C-terminal affected its inhibitory activity significantly. The fusion protein with a natural C-terminal had the inhibitory activity, while no inhibitory activity was detected in the fusion protein with a (His)6-tag at its C-terminal. This implies that extra amino acid residues at the C-terminal of OsWIP1-2 may interfere with its correct folding. The inhibitory assay indicated that the members of rice Bowman-Birk inhibitor gene family probably differenti-ated both in their structure and function.CHENJun LIUJing GUOLei QULijia CHENZhangliang GUHongya 2004Chinese Science Bulletin2004,49,9:7
3Analysis on Mode Field Distribution of Waveguide Grating Coupler by the Scalar FDTD显示文摘The scalar two-dimensional finite difference time domain (FDTD) method is applied to simulate the mode field distribution of TE 0 of the waveguide grating coupler. Computer simulation shows that the same stable mode field distribution pattern is obtained through the different kinds of driving sources. It is found that the optical field mode is determined by waveguide structure and optical wavelength other than the driving source.According to the mode field distribution, the optimum coupling efficiency can be predicted. Compared with another numerical methods,the CPU-time and memory elements of computer used by FDTD are much less.HUANGChong-qing LIUJing LUOWen-hua ANWei-ke YUANXiu-hua HUANGDe-xiu 2003Semiconductor Photonics and Technology2003,9,3:2
4Detection and characterisation of CTX-M and CMY-2 beta-lactamases among Escherichia coli isolates from farm animals in Guangdong province of China显示文摘LIUJ H WEI SY MAJ Y 2007Int J Antimicrob Agents2007,29,5:1
5Rea-l time ultrasound elastography , its potential role in assessment of breast lesions显示文摘Zhu Q L Jiang Y X LiuJ B 2008Ultrasound Med Bioi2008,34,8:1
6Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters 显示文摘LiuJ Lou Y Yokota H Adams PD Kim R Kim SH 2005J Bioi Chern2005,280,15:1
7AnaccumulativeX-raystreakcamerawithsub-600-fstemporalresolutionand50-fstimingjitter显示文摘LiuJ WangJ ShanB etal 2003ApplPhysLett2003,82,20:1
8The controversial links among calorie restriction, SIRTl, and resveratrol显示文摘Hu Y LiuJ WangJ 2011Free Radic Bioi Med2011,51,:1
9Does physical activityduring pregnancy reduce the risk of gestational diabetes amongpreviously inactive women 显示文摘LiuJ Laditka JN Mayer-Davis EJ er al 2008Birth2008,35,3:1
10DevelopingandvalidatingnonlinearheightdiametermodelsformajortreespeciesofOntario’sborealforest显示文摘PengC ZhangL LiuJ 2011NorthernJournalofAppliedForestry2011,18,:1
11Design and full scale test of the fuel handling system显示文摘LIUJ G XIAO H L LI C P 2002Nuclear Engineering and Design2002,218,:1
12Research of a high-current repetitive triggered spark-gap switch and its application显示文摘Cheng X B LiuJ L Qian B L 2010IEEE Transactions on Plasma Science2010,38,3:1
13Loquatleaftriterpenoidacidinratpulmonaryfibrosispreventionandresistanceoflipidperoxideeffect[J]显示文摘LIUJ HUANGY ZHANGL 中国药学杂志0,,:1
14CombinationofplasmamicroRNAswithserumCA19-9forearlydetectionofpancreaticcancer显示文摘LiuJ GaoJ DuY etal 2012IntJCancer2012,131,3:1
15Recent advances in the design of magnetic molecules for use as cryogenic magnetic coolants 显示文摘LIUJ L CHEN Y C GUO F S 2014CoordChem Rev2014,281,:1
16A novel method to prepare nanostructuredmanganese dioxide and its electrochemical properties as a supercapacitor electrode显示文摘Jiang R R Huang T LiuJ L etal 2009Electrochim Acta2009,54,11:1
17Surface modelling of soil pH 显示文摘SHIWJ LIUJ Y DUZP 2009Geoderma2009,150,12:1
18A multiplex RT-PCR for detection of type A influenza virus and differentiation of avian H5, HT, and H9 hemagglutinin subtypes 显示文摘XIEZ X PANG YS LIUJ B 2006Molecular and Cellular Probes2006,20,:1
19Microstructure and mechanical properties of friction stir weldedJoints in 2219- T6 aluminum alloy显示文摘XU W F LIUJ H LUAN G H 2009Materials and Design2009,30,:1
20查看详情显示文摘ZhangWX LiuJ WuG 0,,:1
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