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| 1 | Effects of Exposure to Lead and Cadmium on the Oxidative Damage of Livers in Laying Hens显示文摘[Objective] To detect the effects of exposure to lead and cadmium on the oxidative damage of livers in laying hens. [Methods] One hundred and twenty 40-week-old Hyline brown hens were randomly divided into four groups. 100 mg / L Pb and / or 50 mg / L Cd was added into the drinking water for eight weeks. [Results] Compared with control group,AST and ALT activities in Pb group enhanced; but there were no significant differences. AST and ALT activities in Cd group and( Pb + Cd) group significantly or extremely significantly increased( P < 0. 05 or P < 0. 01). SOD activity,GSH- Px activity and GSH content in( Pb + Cd) group,Cd group and Pb group were significantly or extremely significantly lower than those in control group( P <0. 05 or P <0. 01). Among them,( Pb + Cd) group showed the greatest reduction( P <0. 01). MDA contents in the three groups were significantly higher than that of control group; and( Pb +Cd) group was significantly higher than Pb group and Cd group. Cu,Fe and Zn contents in three groups were higher than those in control group in different degrees( P <0. 05 or P <0. 01). Se contents in Cd group and( Pb + Cd) group were significantly lower than that in control group( P <0. 01). Residue contents in livers in Pb group and Cd group were significantly greater than that in control group; while residue content in( Pb + Cd) group was significantly higher than those in Pb group and Cd group. Ultrastructure showed that there were symptoms of mitochondrial swelling and fractured cristae in liver cells of laying hens after the exposure to Cd and Pb. In( Pb + Cd) group,these symptoms were even greater. [Conclusion] Oxidative damage and disturbance of trace element metabolism were one of the mechanisms for hepatotocity in laying hens induced by Pb and Cd,and synergistic effect lied in the coadministration. | Chen Dawei Pu Junhua Tang Xiujun Lu Junxian Liu Yinyin Jia Xiaoxu Ge Qinglian Gao Yushi | 2014 | Animal Husbandry and Feed Science2014，6，5： | 1 |
| 2 | Identification of Avian-Derived Ingredients in Livestock and Poultry Meat by PCR Technology显示文摘In order to establish a quick and specific method which could identify the avian-derived ingredients,this study used 16 S rRNA gene sequence as target site,and designed the specific primers of chicken,pigeon meat and quail meat. The DNA of common livestock and poultry meat( including mutton,beef,pork,rabbit meat,pigeon meat,quail meat,chicken,duck and goose) was used as template. Though PCR amplification and specific detection,a quick determination method was established to identify the avian-derived ingredients. The results showed that the selected primers could identify the ingredients of animal origin effectively and quickly. The method was convenient and concise,and could detect the chicken-derived,pigeon-derived,quail-derived ingredients in livestock and poultry food quickly and accurately. | Jingxin ZHANG Yushi GAO Xiujun TANG Yanfeng FAN Mengjun TANG Dawei CHEN Qinglian GE Rong GU | 2016 | Asian Agricultural Research2016，8，12： | 0 |
| 3 | Residual Regularity in Tissues of Layer after Combined Exposure to Lead(Pb)and Cadmium(Cd)显示文摘[Objective]The study was to investigate residual regularity in tissues of layer after combined exposure to lead (Pb)and/or cadmium (Cd).[Method]Lead acetate (100 mg/L)and/or cadmium chloride (50 mg/L)were administered in drinking water to 120 individuals of 40-week-old hyline layers for 8 weeks.[Result]Compared with the Cd group,the Cd content in various tissues,except kidney and femur,in Cd+Pb group all increased,but the difference was not significant (P>0.05).The Cd contents in kidney and femur in Cd group were significantly higher than that in Cd+Pb group.Compared with the group Pb,the Pb contents in breast muscle and kidney in Cd+Pb group decreased significantly (P<0.05);the Pb contents in femur decreased extremely (P<0.01);while the Pb contents in leg muscle,heart and spleen increased significantly (P<0.05);the Pb contents in liver and glandular stomach were almost the same.Compared with the control group,the Cd content in various tissues in Pb group increased significantly (P<0.05);no significant changes were observed for Pb content in Cd group.[Conclusion]Deposit of Pb and Cd in poultry body had complex interaction. | Chen Dawei Liu Yinyin Wang Qianqian Pu Junhua Ma Lina Ge Qinglian Tang Xiujun Gao Yushi | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 4 | Complete Sequence Determination and Origin of Mitochondrial DNA Control Region in Dehua Black Chicken显示文摘Primers were designed according to the genomic DNA of red jungle fowl,and the complete sequence of mt DNA D-loop region in Dehua black chicken was obtained.Combined with the D-loop region complete sequence of red jungle fowl in Gen Bank,the maternal origin of Dehua black chicken was explored.The results showed that the complete sequence of mt DNA D-loop region in Dehua black chicken was 1 231 bp,and the average contents of T,C,A and G were33.5%,26.5%,26.7% and 13.3%,respectively.Phylogenetic analysis showed that Dehua black chicken clustered with red jungle fowl (Gallus gallus spadiceus),suggesting Dehua black chicken probably originated from G.g.spadiceus in Yunnan Province or nearby areas. | Lu Junxian Jia Xiaoxu Tang Xiujun Fan Yanfeng Gu Rong Ge Qinglian Gao Yushi | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 5 | CPSF4 promotes triple negative breast cancer metastasis by upregulating MDM4显示文摘Dear Editor,Breast cancer(BrC)is the most common cancer in women.Triple negative BrC(TNBC)is the subtype with highly aggressive clinical behaviors and heterogeneity.Metastasis is the leading cause of TNBC-related deaths,but its mechanism is not well-understood.Apart from PIK3CA,TP53,and PTEN,few recurrent mutations have been identified in TNBC so far,suggesting that TNBC phenotype could be driven by nongenetic alterations such as aberrant expression of oncogenes. | Kaping Lee Qiufan Zheng Qianyi Lu Fei Xu Ge Qin Qinglian Zhai Ruoxi Hong Miao Chen Wuguo Deng Shusen Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 0 |
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