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| 1 | Characterization of edible bird’s nest by peptide fingerprinting with principal component analysis显示文摘OBJECTIVES:Proteins are the major component and play a key role in nutritious and therapeutic functions of edible bird’s nest(EBN);however,limited studies have been conducted on the protein due to difficulties in extraction,isolation as well as identification.This study aimed to provide comprehensive information for the quality evaluation of EBN peptides,which would be a valuable reference for further study on EBN proteins.METHODS:Here,we developed a quality control method using high performance liquid chromatography(HPLC)peptide fingerprints deriving from EBN being digested with simulated gastric fluid.The characteristic peptide peaks were collected and identified by LC-MS/MS.RESULTS:The characteristic peptide peaks,corresponding to the protein fragments of acidic mammalian chitinase-like,lysyl oxidase,and Mucin-5AC-like,were identified and quantified.Interestingly,the principal component analysis indicated that the fingerprints were able to discriminate colour of EBN(white/red)and production sites(cave/house)of White EBN on the same weight basis.As proposed by the model developed in this study,Muc-5AC-like and AMCaselike proteins were the markers with the highest discriminative power.CONCLUSIONS:The overall findings suggest that HPLC peptide fingerprints were able to clearly demonstrate peptide profile differences between genuine and adulterated EBN samples;and classify EBN samples by its color and production site.In addition,the protein identification results suggested that Muc-5AC-like protein was the major protein in EBN. | Chun-Fai Wong Gallant Kar-Lun Chan Ming-Lu Zhang Ping Yao Huang-Quan Lin Tina Ting-Xia Dong Geng Li Xiao-Ping Lai Karl Wah-Keung Tsim | 2017 | Food Quality and Safety2017，1，1： | 2 |
| 2 | Vasopressin deficiency contributes to the vasodilation of septic shock 显示文摘 | Landry DW Levin HR Gallant EM | 1997 | Circulation1997，95，5： | 1 |
| 3 | Estimation of stochastic volatility models with diagnostics显示文摘 | Gallant A R Hsieh D Tauchen G | | 0，，01： | 1 |
| 4 | Alternative models for stock price dynamics显示文摘 | Chernov M Gallant A R Ghysels E | 2003 | Journal of Econo- metrics2003，116，： | 1 |
| 5 | Renal function with use of a tenofovir containing initial antiretroviral regimen显示文摘 | Gallant JE Moore RD | 2009 | AIDS2009，23，15： | 1 |
| 6 | Changes in renal function associated with tenofovir disoproxil fumarate treatment, compared with nucleoside reverse-transcriptase inhibitor treatment 显示文摘 | Gallant JE Parish MA Kemly JC | 2005 | Clin Infect Dis2005，40，8： | 1 |
| 7 | Recovery characteristics following induction of anaesthesia with a combination of thiopentone andpropofol 显示文摘 | Rashiq S Gallant B Grace M | 1994 | Can J Anaesth1994，41，12： | 1 |
| 8 | When to start antiret roviral therapy? NA-ACCORD stim- ulates the debate显示文摘 | Gallant JE | 2009 | AIDS Read2009，19，2： | 1 |
| 9 | On the basis in flexible functional form and an essentially unbiased form: the flexible Fourier form 显示文摘 | Gallant R | 1981 | Journal of Econometrics1981，15，： | 1 |
| 10 | CIA,a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant显示文摘 | Sauvé F McBroom LD Gallant J | | 0，，1： | 1 |
| 11 | Pseudomonas aeru- ginosa cystic fibrosis clinical isolates produce exotoxin A with altered ADP-ribosyhransferase activity and cytotoxicity 显示文摘 | Gallant CV Raivio TL Olson JC | 2000 | Microbiology2000，146，8： | 1 |
| 12 | Stock prices and volume显示文摘 | Gallant R | 1992 | Review of Financial Studies1992，，5： | 1 |
| 13 | Angiographic management of traumatic arteriovenous fistulas:clinical results显示文摘 | CLARK R A GALLANT T E ALEXANDER E S | 1983 | Radiology1983，147，1： | 1 |
| 14 | Malignant hyperthermia and porcine stress syndrome: a tale of two species 显示文摘 | Louis C F Gallant E M Remple E | 1990 | Pig News and Information1990，11，3： | 1 |
| 15 | Vasopressin deficiency contributes to the vasodilation of septic shock显示文摘 | Landry DW Levin HR Gallant EM | 1997 | Circulation1997，95，： | 1 |
| 16 | Vasopressin pressorhypersensitivity in vasodilatory septic shock显示文摘 | Landry D W Levin D R Gallant E M | 1997 | Crit Care Med1997，25，8： | 1 |
| 17 | Stock price and volume 显示文摘 | GALLANT A R PETER E R GEORGE T | 1992 | Review of Financial Studies1992，5，： | 1 |
| 18 | Identification of a novel vertebrate cyclin:cyclin B3 shares properties with both A-and Btype cyclins显示文摘 | Gallant P Nigg E A | | 0，，03： | 1 |
| 19 | Angiographic management of traumatic arteriovenous fistulas:clinical results显示文摘 | Clark RA Gallant TE Alexander ES | 1983 | Radiology1983，147，1： | 1 |
| 20 | Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish显示文摘 | Douglas S E Gallant J W Liebscher R S | 2003 | Dev Comp Immunol2003，27，： | 1 |
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