|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Strain and medium-related variability in the yeast-mycelial transition of Aureobasidium pullulans显示文摘 | Gadd G M Cooper L A | 1984 | FEMS Microbiology Letters1984，23，1： | 1 |
| 3 | The chang role of purchasing:eeconsidering three strategic issues显示文摘 | GADDE L E HAKANSSON H H | 1994 | European Journal of Purchasing & Supply Management1994，，1： | 1 |
| 4 | System sourcing - opportunities and problems 显示文摘 | Gadde L - E Jellbo O | 2002 | European Journal of Purchasing & Supply Management2002，8，1： | 1 |
| 5 | The construction industry as a loosely coupled system: implications for productivity and innovation显示文摘 | DUBOIS A GADDE L E | 2002 | Conslruction Management and Economics2002，20，7： | 1 |
| 6 | New mechanisms for PRLr action in breast cancer显示文摘 | CLEVENGER C V GADD S L ZHENG J | 2009 | Trends Endocrinol Metab2009，20，5： | 1 |
| 7 | La Dynamique des Rfiseaux de Distri- bution: Implications Pour Les Interm6diaires显示文摘 | GADDE L E | 2012 | Re- vue Management i Avenir2012，51，1： | 1 |
| 8 | Reduction and precipitation of chromate by mixed culture sulphate-reducing bacterial biofilms显示文摘 | Smith W L Gadd G M | 2000 | Journal of Applied Microbiology2000，88，6： | 1 |
| 9 | Making the most of supplier rela- tionships显示文摘 | Gadde L E Snehota I | 2000 | Industrial Marketing Management2000，29，4： | 1 |
| 10 | Making the most of supplier relation- ships显示文摘 | Gadde L Snehota I | 2000 | Industrial Marketing Management2000，29，4： | 1 |
| 11 | Acetaminophen-induced proliferation of estrogen-responsive breast cancer cells is associated with increases in c-myc RNA espression and NF-kappaB activity显示文摘 | Gadd S L Hobbs G Miller M R | 2002 | Toxicol Sci2002，66，2： | 1 |
| 12 | Surgical management of phyllodes tumors显示文摘 | Mangi A A Smith B L Gadd M A | 1999 | Arch Surg1999，134，5： | 1 |
| 13 | Delayed Gastric Emptying After Pancreaticoduodenectomy:Is Subtotal Stomach Preserving Better or Pylorus Preserving?显示文摘 | Hanna M Gadde R Tamariz L | 2015 | J Gastrointest Surg2015，19，8： | 1 |
| 14 | Reduction and precipitation of chromate by mixed culture sulphate-reducing bacterial biofilms 显示文摘 | Smith W L Gadd G M | 2000 | J Appl Microbi- ol2000，88，： | 1 |
| 15 | Making the most of supplier relationships 显示文摘 | Gadde L Snehota I | 2000 | Industrial Marketing Management2000，29，： | 1 |
| 16 | Making the most of supplier relationships 显示文摘 | Gadde L Snehota I | 2000 | Industrial Marketing Management2000，29，4： | 1 |
| 17 | Improving Logistics Outsourcing through Increasing Buyer-provider Interaction 显示文摘 | Gadde L E Huhhen K | 2009 | Industrial Marketing Management2009，，38： | 1 |
| 18 | Making the most of supplier rela- tionships显示文摘 | GADDE L E SNEHOTA | 2000 | Industrial Marketing Management2000，29，4： | 1 |
| 19 | Zinc uptake and toxicity in the yeast Sporobolomyces roseus and Saccharomyces cerevisiae显示文摘 | Mowll J L Gadd G M | 1983 | J Gen Microbiol1983，129，： | 1 |
| 20 | The changing role of purchasing: Reconsid- ering three strategic issues显示文摘 | Gadde L E Hakansson H | 1994 | European Journal of Purchasing And Supply Management1994，1，94： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员