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		题名	作者	年代	出处	被引量
	1	Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation显示文摘AIM: To investigate whether hepatocytes isolated from macroscopically normal liver during hepatic resection for neoplasia could provide a novel source of healthy hepatocytes, including the development of reliable protocols for malignant cells removal from the hepatocyte preparation. METHODS: Hepatocytes were procured from resected liver of 18 patients with liver tumors using optimised digestion and cell-enrichment protocols. Suspensions of various known quantities of the HT-29 tumor cell line and patient hepatocytes were treated or not with Ep-CAM-antibody-coated immunomagnetic beads in order to investigate the effi cacy of tumor-purging by immunomagnetic depletion, using a semi-quantitative RT-PCR method developed to detect tumor cells. Immunomagnetic bead-treated or bead-untreated tumor cell-hepatocyte suspensions were transplanted intra-peritoneally in Balb/C nude mice to assess the rates of tumor development. RESULTS: Mean viable hepatocyte yield was 9.3 x 106 cells per gram of digested liver with mean viability of 70.5%. Immunomagnetic depletion removed tumor cells to below the RT-PCR detection-threshold of 1 tumor cell in 106 hepatocytes, representing a maximum tumor purging efficacy of greater than 400 000-fold. Transplanted, immunomagnetic bead-purged tumor cell-hepatocyte suspensions did not form peritonealtumors in Balb/C nude mice. Co-transplantation of hepatocytes with tumor cells did not increase tumorigenesis of the tumor cells. CONCLUSION: Immunomagnetic depletion appears to be an effective method of purging contaminating tumor cells to below threshold for likely tumorigenesis. Along with improved techniques for isolation of large numbers of viable hepatocytes, normal liver resected for neoplasia has potential as another clinically useful source of hepatocytes	Aravin Gunasegaram Javed Akhter Peng Yao Loreena A Johnson Stephen M Riodan David L Morris	2008	World Journal of Gastroenterology2008，14，32：	2
	2	Hepatocytetransplantation:a review of laboratory techniques and clinical ex-periences显示文摘	Akhter J Johnson LA Gunasegaram A	2007	Surgeon2007，5，3：	1
	3	A Review for Mobile Commerce Research and Applications 显示文摘	Ngai E W T Gunasegaram A	2007	Decision Support Systems2007，43，1：	1
	4	Hepatocyte transplantation:a review of laboratory techniques and clinical experiences显示文摘	Akhter J Johnson LA Gunasegaram A		0，，3：	1
	5	Novel surgical management of renal trauma:InLine radiofrequency ablation coagulation显示文摘	Yao P Gunasegaram A Ladd LA		0，，：	1
	6	A relationship between sperm countandintracellular progesterone,17 alphahydroxy progesterone,and cortisolconcentrations inhuman spermatozoa 显示文摘	Loganath A Gunasegaram R Peh KL	1994	Fertil Steril1994，61，5：	1
	7	A delta 4-3-keto pathway fortestosteronesynthesis in the human spermatozoa显示文摘	Gunasegaram R Chew PC Loganath A	1998	Arch Androl1998，40，1：	1
	8	Performance Measures and Metrics in a Supply Chain Environment 显示文摘	GUNASEGARAM A PATEL C TIRTIROGLU E	2001	Interna- tional Journal of Operations and Product ion Management2001，，21：	1

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