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| 1 | 未处理或上游法处理后精子的DNA碎片能否预测使用赠卵ICSI后的妊娠?显示文摘本研究比较了未处理的精子和上游法处理后精子中的DNA碎片值(SDF)预测使用赠卵ICSI后妊娠的可能性。总共81例不孕女性接受通过夫精和赠卵ICSI后的胚胎移植。这种方式排除了目前公认的影响精子DNA修复的女方因素。在ICSI受精时,同时使用DNA碎片率检测试剂盒(Halosperm)进行盲法检测未处理的和上游法处理后精液中的精子DNA碎片。经上游法处理后的精液样本中SDF值呈显著下降。有趣的是,未处理的或上游法处理后精液样本中的SDF值均能预测妊娠。通过ROC曲线和其衍生的约登指数计算得出未处理前和上游法处理后精子sDF的临界值分别是24.8%和17.5%。未处理精子SDF预测妊娠的敏感性和特异性分别为75%和69%,而上游法处理后的精子SDF预测妊娠的敏感性和特异性分别是78%和73%。虽然SDF值增高与生育呈负相关,但我们发现在精子筛选降低sDF值后再与赠卵进行ICSI并不能保证获得妊娠。这表明目前的技术手段对于一些DNA损伤仍无法探及,从而相对影响辅助生殖成功的机率。因此,我们认为对目前的“冰山模式”作一些修饰后可以用来解释SDF在生育中的作用。 | Jaime Gosálvez Pedro Caballero Carmen López-Fernández Leonor Ortega José Andrés Guijarro José Luís FernAndez Stephen D Johnston Rocio Nufiez-Calonge | 2013 | Asian Journal of Andrology2013，15，6： | 3 |
| 2 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 3 | Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence显示文摘 | Juan C. Acosta Ana O’Loghlen Ana Banito Maria V. Guijarro Arnaud Augert Selina Raguz Marzia Fumagalli Marco Da Costa Celia Brown Nikolay Popov Yoshihiro Takatsu Jonathan Melamed Fabrizio d’Adda di Fagagna David Bernard Eva Hernando Jesús Gil | 2008 | Cell2008，，6： | 2 |
| 4 | Ruthenium-catal- ysed asymmetric transfer hydrogenation of N-(tert-hu- tanesulfinyl) imines显示文摘 | GUIJARRO D PABLO O YUS M | 2009 | Tetrahedron Lett2009，50，38： | 1 |
| 5 | Generation of allylic and benzylic organolithium reagents from the corresponding ester, amide, carbonate, carbamate and urea derivatives显示文摘 | M Alonso D Guijarro M Yus | 1999 | Tetrahedron1999，55，11： | 1 |
| 6 | Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence显示文摘 | Juan C. Acosta Ana O’Loghlen Ana Banito Maria V. Guijarro Arnaud Augert Selina Raguz Marzia Fumagalli Marco Da Costa Celia Brown Nikolay Popov Yoshihiro Takatsu Jonathan Melamed Fabrizio d’Adda di Fagagna David Bernard Eva Hernando Jesús Gil | 2008 | Cell2008，，6： | 1 |
| 7 | Simultaneous detection of Aeromonas salrnonicida, Flavobacterium psychrophilum ,and Yersinia ruckeri, three major fish pathogens,by multiplex PCR显示文摘 | Cerro D A Marquez I Guijarro J A | 2002 | Applied and Environ- mental Microbiology2002，68，10： | 1 |
| 8 | Preparation and use of aziridino alcohols as promoters for the enantioselective addition of dialkylzinc reagents to N-(diphenylphosphinoyl) imines显示文摘 | ANDERSSON P G GUIJARRO D TANNER D | 1997 | J Org Chem1997，62，21： | 1 |
| 9 | Enantioselective addition of dialkylzinc reagents to N-(diphenylphos-phinoyl) imines promoted by 2-azanorbornylmethanols 显示文摘 | GUIJARRO D PINHO P ANDERSSON P G | 1998 | J Org Chem1998，63，8： | 1 |
| 10 | Generation of allylic and benzylic organolithium reagents from the corresponding ester,amide, carbonate, carbamate and urea derivatives 显示文摘 | Alonso M Guijarro D Yus M | 1999 | Tetrahedron1999，5，11： | 1 |
| 11 | Preparation and use of aziridino alcohols as promoters for the enan- tioselective addition of aialkylzlnc reagents to N-( diphenylphosphinoyl) imines 显示文摘 | P G Andersson D Guijarro D Tanner | 1997 | J Org Chem1997，62，： | 1 |
| 12 | Enantioselective addition of dialkylzinc reagents to N-( diphenylphosphinoyl) imines promoted by 2-azanorbornylmethanols 显示文摘 | D Guijarro P Pinho P G Andersson | 1998 | J Org Chem1998，63，： | 1 |
| 13 | Comparative analysis and mutation effects offpp2-fppl tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum显示文摘 | Pérez-Pascual D Gómez E (A)lvarez B Méndez J Reimundo P Navais R Duchaud E Guijarro J | | 0，，： | 1 |
| 14 | 显示文摘 | Guijarro D Yus M | 1999 | Tetrahedron1999，55，11： | 1 |
| 15 | Simultaneous detection ofAeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR显示文摘 | Cerro A D Marquez 1 Guijarro J A | 2002 | Appled and Environmental Microbiology2002，68，10： | 1 |
| 16 | Preparation and use of aziridino alcohols as promoters for the enantioselective addition of dialkylzinc reagents to N-(diphenylphosphinoyl) imines显示文摘 | Anderson P G Guijarro D Tanner D | 1997 | J Org Chem1997，62，21： | 1 |
| 17 | Trends in the management and outcomes of acute pulmonary embolism:analysis from the RIETE registry显示文摘 | Jiménez D De Miguel-Diez J Guijarro R | 2016 | J Am Coll Cardiol2016，67，2： | 1 |
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