|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 未处理或上游法处理后精子的DNA碎片能否预测使用赠卵ICSI后的妊娠?显示文摘本研究比较了未处理的精子和上游法处理后精子中的DNA碎片值(SDF)预测使用赠卵ICSI后妊娠的可能性。总共81例不孕女性接受通过夫精和赠卵ICSI后的胚胎移植。这种方式排除了目前公认的影响精子DNA修复的女方因素。在ICSI受精时,同时使用DNA碎片率检测试剂盒(Halosperm)进行盲法检测未处理的和上游法处理后精液中的精子DNA碎片。经上游法处理后的精液样本中SDF值呈显著下降。有趣的是,未处理的或上游法处理后精液样本中的SDF值均能预测妊娠。通过ROC曲线和其衍生的约登指数计算得出未处理前和上游法处理后精子sDF的临界值分别是24.8%和17.5%。未处理精子SDF预测妊娠的敏感性和特异性分别为75%和69%,而上游法处理后的精子SDF预测妊娠的敏感性和特异性分别是78%和73%。虽然SDF值增高与生育呈负相关,但我们发现在精子筛选降低sDF值后再与赠卵进行ICSI并不能保证获得妊娠。这表明目前的技术手段对于一些DNA损伤仍无法探及,从而相对影响辅助生殖成功的机率。因此,我们认为对目前的“冰山模式”作一些修饰后可以用来解释SDF在生育中的作用。 | Jaime Gosálvez Pedro Caballero Carmen López-Fernández Leonor Ortega José Andrés Guijarro José Luís FernAndez Stephen D Johnston Rocio Nufiez-Calonge | 2013 | Asian Journal of Andrology2013，15，6： | 3 |
| 2 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 3 | Real-time image processing for crop/weed discrimination in maize fields显示文摘 | Xavier P. Burgos-Artizzu Angela Ribeiro Maria Guijarro Gonzalo Pajares | 2010 | Computers and Electronics in Agriculture2010，，2： | 2 |
| 4 | Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence显示文摘 | Juan C. Acosta Ana O’Loghlen Ana Banito Maria V. Guijarro Arnaud Augert Selina Raguz Marzia Fumagalli Marco Da Costa Celia Brown Nikolay Popov Yoshihiro Takatsu Jonathan Melamed Fabrizio d’Adda di Fagagna David Bernard Eva Hernando Jesús Gil | 2008 | Cell2008，，6： | 2 |
| 5 | Transcription factor-kappaB(NF-kappaB) and renal disease显示文摘 | Guijarro C Egido J | | 0，，： | 2 |
| 6 | N-acetyl-L-cysteine combined with mesalamine in the treatment of ulcerative colitis: Randomized,placebo-controlled pilot study显示文摘AIM: To evaluate the effectiveness and safety of oral N-acetyl-L-cysteine (NAC) co-administration with mesalamine in ulcerative colitis (UC) patients. METHODS: Thirty seven patients with mild to moderate UC were randomized to receive a four-wk course of oral mesalamine (2.4 g/d) plus N-acetyl-L-cysteine (0.8 g/d) (group A) or mesalamine plus placebo (group B). Patients were monitored using the Modified Truelove-Witts Severity Index (MTWSI). The primary endpoint was clinical remission (MTWSI ≤ 2) at 4 wk. Secondary endpoints were clinical response (defined as a reduction from baseline in the MTWSI of ≥ 2 points) and drug safety. The serum TNF-α, interleukin-6, interleukin-8 and MCP-1 were evaluated at baseline and at 4 wk of treatment. RESULTS: Analysis per-protocol criteria showed clinical remission rates of 63% and 50% after 4 wk treatment with mesalamine plus N-acetyl-L-cysteine (group A) and mesalamine plus placebo (group B) respectively (OR = 1.71; 95% CI: 0.46 to 6.36; P = 0.19; NNT = 7.7). Analysis of variance (ANOVA) of data indicated a significant reduction of MTWSI in group A (P = 0.046) with respect to basal condition without significant changes in the group B (P = 0.735) during treatment. Clinical responses were 66% (group A) vs 44% (group B) after 4 wk of treatment (OR = 2.5; 95% CI: 0.64 to 9.65; P = 0.11; NNT = 4.5). Clinical improvement in group A correlated with a decrease of IL-8 and MCP-1. Rates of adverse events did not differ significantly between both groups. CONCLUSION: In group A (oral NAC combined with mesalamine) contrarily to group B (mesalamine alone), the clinical improvement correlates with a decrease of chemokines such as MCP-1 and IL-8. NAC addition not produced any side effects. | Luis G Guijarro Jose Mate Javier P Gisbert Jose Luis Perez-Calle Ignacio Marín-Jimenez Encarna Arriaza Tomás Olleros Mario Delgado Maria S Castillejo David Prieto-Merino Venancio Gonzalez Lara Amado Salvador Pea | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 7 | Central role of the transcription factor nuclear factorkappa B in mesangial cell production of chemokines显示文摘 | Guijarro C | 1997 | Contrib Nephrol1997，120，6： | 1 |
| 8 | Transcription factor-kB (NF-kB) and renal disease显示文摘 | Guijarro C Egido J | | 0，，02： | 1 |
| 9 | Prevalence of carbohydrate metabolism disturbances in a population of children and adolescents with severe obesity显示文摘 | GUIJARRO DE ARMAS MG MEGIAS S | | 0，，10： | 1 |
| 10 | Simultaneous detection of Aeromonas salmonicida , Flavobacterium psychrophilum , and Yersinia ruckeri , three major fish pathogens, by multiplex PCR显示文摘 | del Cerro A Marquez I Guijarro J A | 2002 | Appl Environ Microbiol2002，68，10： | 1 |
| 11 | Develop- ment and field test of an on-line computerized instrumenta- tion system for air velocity, temperature and differential pressure measurements in poultry houses 显示文摘 | Blanes Vidal V Guijarro E Nadimi A G | 2010 | Spanish Journal of Agricultural Research2010，8，3： | 1 |
| 12 | The cen tral role of nuclear factor-kappa B in mesangial cell activation显示文摘 | Massy ZA Guijarro C Donnell MP | 1999 | Kidney Int Suppl1999，71，： | 1 |
| 13 | Chronic Renal Allograft Rejection:Immunologic and Nonimmunologic Risk Factors显示文摘 | Massy Z A Guijarro C Wiederkehr M R | 1996 | Kidney Int1996，49，： | 1 |
| 14 | Amyloid fibril formation by an SH3 domain显示文摘 | Guijarro J I Sunde M Jones J A | 1998 | Proe Natl Aead Sei U S A1998，95，： | 1 |
| 15 | Central 1 role of nuclear factor kappa B NF-κB in mesangial cell production of chemokines 显示文摘 | Guijarro C Kim Y Kasiske BL | 1997 | Contribul Nephrol1997，120，： | 1 |
| 16 | Identification of specific in vivo-induced (ivi) genes in Yersinia ruckeri and analysis of ruckerbactin, a catecholate siderophore iron acquisition system 显示文摘 | FERNANDEZ L MARQUZ I GUIJARRO J A | 2004 | Appl Environ Microbiol2004，70，9： | 1 |
| 17 | High-- sensitivity C-- reactive protein: potential adjunct for global risk assessment in the primary prevention of cardiovascular disease显示文摘 | Guijarro C | 2001 | Circulation2001，104，22： | 1 |
| 18 | Selectivity of diamond- and squaremesh codends in the deepwater crustacean trawl fishery off the Balearic Islands (western Mediterranean) 显示文摘 | Guijarro B Massuti E | 2006 | ICES J Mar Sci2006，63，1： | 1 |
| 19 | Central Role of the Transcription Factor Nuclear Factor Kappa-B in Mesangial Cell Production of Chemokines显示文摘 | Guijarro C | 1997 | Contrib Nephrol1997，120，： | 1 |
| 20 | Interoperability frameworks and enter- prise architectures in e-governm entinitiatives in europe and the united states 显示文摘 | Luis Guijarro | 2007 | Government Information Quarterly2007，24，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员