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| 1 | Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness显示文摘Tonic signaling of chimeric antigen receptor(CAR),i.e.,the spontaneous CAR activation in the absence of tumor antigen stimulation,is considered to be a pivotal event controlling CAR-T efficacy.However,the molecular mechanism underlying the spontaneous CAR signals remains elusive.Here,we unveil that positively charged patches(PCPs)on the surface of the CAR antigenbinding domain mediate CAR clustering and result in CAR tonic signaling.For CARs with high tonic signaling(e.g.,GD2.CAR and CSPG4.CAR),reducing PCPs on CARs or boosting ionic strength in the culture medium during ex vivo CAR-T cell expansion minimizes spontaneous CAR activation and alleviates CAR-T cell exhaustion.In contrast,introducing PCPs into the CAR with weak tonic signaling,such as CD19.CAR,results in improved in vivo persistence and superior antitumor function.These results demonstrate that CAR tonic signaling is induced and maintained by PCP-mediated CAR clustering.Notably,the mutations we generated to alter the PCPs maintain the antigen-binding affinity and specificity of the CAR.Therefore,our findings suggest that the rational tuning of PCPs to optimize tonic signaling and in vivo fitness of CAR-T cells is a promising design strategy for the nextgeneration CAR. | Jian Chen Shizhen Qiu Wentao Li Kun Wang Yu Zhang Han Yang Baichuan Liu Guangfei Li Li Li Min Chen Junjie Lan Jiahua Niu Peijie He Lei Cheng Gaofeng Fan Xin Liu Xianmin Song Chenqi Xu Haitao Wu Haopeng Wang | 2023 | Cell Research2023，33，5： | 2 |
| 2 | Blind zone-suppressed hybrid beam steering for solid-state Lidar显示文摘We demonstrate a blind zone-suppressed and flash-emitting solid-state Lidar based on lens-assisted beam-steering technology.As a proof-of-concept demonstration,with the design of a subwavelength-gap 1D long-emitter array and multiwavelength flash beam emitting,the device was measured to have 5%blind zone suppression,0.06°/point-deflection step,and 4.2μs scanning speed.In time-of-flight ranging experiments,Lidar systems have a field of view of 11.3°×8.1°(normal device)or 0.9°×8.1°(blind-zone suppressed device),far-field number of resolved points of 192,and a detection distance of 10 m.This work demonstrates the possibility that a new integrated beam-steering technology can be implemented in a Lidar without sacrificing other performance. | CHAO LI XIANYI CAO KAN WU GAOFENG QIU MINGLU CAI GUANGJIN ZHANG XINWAN LI JIANPING CHEN | 2021 | Photonics Research2021，9，9： | 1 |
| 3 | Comparative analysis of the morphology,karyotypes and biochemical composition of muscle in Siniperca chuatsi,Siniperca scherzeri and the F1 hybrid(S.chuatsi♀×S.scherzeri♂)显示文摘Combining morphological comparison with karyotyping can make a more accurate and comprehensive study of mandarin fish(Siniperca spp.)and hybrid progenies.In recent years,the domestication of hybrid mandarin fish has become a hot spot in China,and it is essential to evaluate the nutritional value of the fish after food conversion.In this study,the F1 hybrid(S.chuatsi♀×S.scherzeri♂)were fed with artificial feed for five months.Afterwards,we studied the morphology,karyotypes,and biochemical composition of muscle in the F1 hybrid and its parents.The morphological results showed that total length and body height of the F1 hybrid appear to derive more from S.chuatsi than S.scherzeri.The DNA ploidy and karyotype results indicated that the F1 hybrid have a diploid chromosome number(2n=48)and the same karyotypes(2n=4sm+14th+30t,NF=52)with its parents,suggesting that the chromosome of the mandarin fish has convergence in the process of evolution.The results of biochemical composition of muscle showed that there were no significant difference in other nutritional indicators(P>0.05),except the Gly(glycine)content of the F1 hybrid was significantly higher than that of S.chuatsi(P<0.05)under the experimental feeding regime,suggesting that the nutritional value of the F1 hybrid with artificial feeding culture is also higher.The study is expected to provide a reference for further research on the domestication,classification,chromosomes,and nutritional requirements of mandarin fish. | Wenzhi Guan Gaofeng Qiu Feng Liu | 2022 | Aquaculture and Fisheries2022，7，4： | 1 |
| 4 | Cathepsin C transcripts are dif-ferentially expressed in the final stages of oocyte maturation in ku-ruma prawn Marsupenaeus japonicus 显示文摘 | Qiu Gaofeng Yamano K Unuma T | 2005 | Comp Biochem PhysiolB:Biochem Mol Biol2005，140，2： | 1 |
| 5 | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii显示文摘The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii. | Xue Liu Lingxia Zhou Biyun Luo Hongli Qian Baoqing Ye Keyi Ma Gaofeng Qiu | 2023 | Aquaculture and Fisheries2023，8，5： | 0 |
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