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		题名	作者	年代	出处	被引量
	1	Evidence from ^(18)O feeding studies for hydroxyl group donor in the reaction catalyzed by cytidylate hydroxymethylase MilA显示文摘5-Hydroxymethylcytosine (5hmC) was present in T-even phage and mammalian DNA. 5hmC in phage is formed by hydroxymethylation of the cytosine base in deoxycytidylate (dCMP) by deoxycytidylate hydroxymethylase (CH), which uses the solvent water as the hydroxyl group donor. By contrast, 5hmC is formed in mammal zygotes by the oxidation of 5-methylcytosine (5mC). 5hmC was also present in a nucleoside antibiotic mildiomycin and its formation is governed by a cytidylate hydroxymethylase MilA. However, the catalytic mechanism remains unknown. In the present study, we purified His-tagged MilA and fed its in vitro reaction with H218O. The LC-MS analysis of the product revealed that 18O was incorporated into the hydroxymethylated CMP (HmCMP), and the secondary MS result of 18O-labeled HmCMP indicated that 18O was incorporated into the cytosine of HmCMP. The results demonstrate that MilA uses solvent water as the hydroxyl group donor like CH. Moreover, Thr102 of MilA was predicted as potential critical amino acid anchoring one molecule of water for hydroxylation. Finally, organizational context comparison in microbial genomes reveals that six homologous ORFs originally annotated as putative thymidylate synthase (TS) are more likely to be CMP hydroxymethylase.	CHEN Cheng GAO TuLing ZHAO Gong DENG ZiXin HU ShenCai XU H Howard HE XinYi	2013	Chinese Science Bulletin2013，58，8：	1

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