|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Assessment of ecosystem services value in response to prevailing and future land use/cover changes in Lahore,Pakistan显示文摘Land use/cover changes(LUCCs)significantly affect ecosystem services(ESs)and their corresponding monetary value.ESs can be evaluated to analyze the ecological and environmental changes caused by LUCCs.This research aims to estimate variations in the ecosystem services value(ESV)due to LUCCs in Lahore of Pakistan,and to offer information and recommendations to policy-makers concerned with the economic improvement of metropolis areas.We first investigated the historical LUCCs from 1990 to 2019,and then simulated the future land use/cover in 2030 and 2050 based on the CA-Markov model under three scenarios,including business-as-usual(BAU),rapid economic growth(REG),and coordinated environmental sustainability(CES).Subsequently,we evaluated the ESV from 1990 to 2050 and evaluated the historical and potential future ESV changes induced by LUCCs during 1990–2019 and 2019–2050,respectively.The results showed that,land use/cover exhibited an increase in built-up land and decreases in vegetation,water body,and unused land both in the past and future.The net ESV decreased from 58.26 million USD in 1990 to 50.31 million USD in 2019.In 2050,the decrease in ESV is most rapid under the REG scenario(decrease ESV of 7.13 million USD and decrease percentage of 14.18%),followed by the BAU(decrease ESV of 5.61 million USD and decrease percentage of 11.15%)and CES(decrease ESV of 5.18 million USD and decrease percentage of 10.30%)scenarios.For each ecosystem service type,the ESV of waste treatment decreased by 18.37%from 1990 to 2019,followed by soil formation and conservation(17.52%),biodiversity and maintenance(13.23%),climate regulation(11.80%),food production(11.69%),hydrological regulation(11.11%),and aesthetic value(3.07%).These outcomes indicate that continuous land use/cover planning should focus on regenerating aquatic areas and vegetation restoration. | Mobeen Akhtar Yuanyuan Zhao Guanglei Gao Qudsia Gulzar Azfar Hussain Abdus Samie | 2020 | Regional Sustainability2020，1，1： | 2 |
| 3 | Spatiotemporal patterns of the forage-livestock balance in the Xilin Gol steppe,China:implications for sustainably utilizing grassland-ecosystem services显示文摘Understanding the spatiotemporal patterns of the forage-livestock balance is imperative for regionally arranging animal husbandry production while ensuring sustainable grassland-ecosystem service use.The Xilin Gol steppe is an important native grassland resource in Inner Mongolia Autonomous Region,China.This study aimed to elucidate the dynamics of the forage-livestock balance in the Xilin Gol steppe during the period 2000–2015.We evaluated the forage production and corresponding livestock carrying capacity(LCC)in the growing seasons of 2000–2015 using remote sensing data and field surveys.The spatiotemporal patterns of the forage-livestock balance were then assessed at regional,city(including city,county and banner),and village scales using statistical and household survey data.The results showed that both forage production and LCC decreased in the Xilin Gol steppe from east to west.During the period 2000–2015,the regional average forage production and corresponding LCC fluctuated without following a distinct trend,but were consistent with the variations in precipitation.The forage-livestock balance varied with time,space,and scale.At the regional scale,steppes were overgrazed in the early 2000s,but a forage-livestock balance or even grazing potential was achieved in other years.At the city scale,approximately half of the region exhibited a'forage-livestock balance'since 2000.However,about half of the region still experienced overgrazing,which mainly located in the southwest sandy zones.Such changes may have been affected by the variations in grassland quality,forage production,compensation payment,and so on.We suggest a location-specific management scheme for grazing constraints,ecological compensation payment,and industry development to aid in harmonizing animal husbandry and environmental restoration,while promoting sustainable development goals by 2030. | QU Yingbo ZHAO Yuanyuan DING Guodong CHI Wenfeng GAO Guanglei | 2021 | Journal of Arid Land2021，13，2： | 2 |
| 4 | Efficient silencing of the multicopy DUX4 gene by ABE-mediated start codon mutation in human embryos显示文摘The Dux gene plays an important role in mouse embryo development(De Iaco et al.,2017;Hendrickson et al.,2017;Whiddon et al.,2017).It participates in the maternal-to-zygotic transition(MTZ),which mainly includes maternal transcript degradation and zygotic genome activation(ZGA)and is the key process for embryonic development.Approximately 11-100 copies of the DUX4 gene form a tandem cluster in the human genome(Snider et al.,2009,2010). | Yubing Liu Xinmei Lu Min Ye Ling Wang Rongxin Tang Zhiyong Yang Bongkoch Turathum Chenchen Liu Yujing Xue Menghua Wu Yannan Yang Ermeng Gao Di Zhang Feng Yang Keh-Kooi Kee Xingxu Huang Guanglei Li Ri-Cheng Chian | 2022 | Journal of Genetics and Genomics2022，49，10： | 1 |
| 5 | A polymer electrolyte with a thermally induced interfacial ion-blocking function enables safety-enhanced lithium metal batteries显示文摘Lithium metal batteries(LMBs)have recently been revitalized as one of the most promising electrochemical energy storage systems,owing to the ultrahigh specific capacity(3860 mAh g^(-1))and ultralow potential(-3.04 V vs.standard hydrogen electrode)of lithium metal anodes.However,safety hazards originating from lithium dendrite growth and pulverization during cycling and thermal stimulation present significant challenges to the practical application of LMBs.To address this issue,we have developed an in situ polymer electrolyte with thermally induced interfacial ion-blocking ability.We demonstrate that the repolymerization and deposition of residual vinylene carbonate in the as-prepared electrolyte under thermal abuse predominantly results in thermally induced ion blocking at the solid electrolyte interface,thus achieving superior LMB safety.The developed polymer electrolyte also yields superior cyclability in LMBs.This design philosophy provides a good paradigm for improving the safety of LMBs. | Huanrui Zhang Lang Huang Hantao Xu Xiaohu Zhang Zhou Chen Chenhui Gao Chenglong Lu Zhi Liu Meifang Jiang Guanglei Cui | 2022 | eScience2022，2，2： | 1 |
| 6 | Rational manipulation of composition and construction toward Zn/Co bimetal hybrids for electromagnetic wave absorption显示文摘Rational manipulation of multi-metal hybrids with controllable compositions and constructions is evolv-ing as an efficacious strategy for tunable electromagnetic(EM)characteristics and EM wave(EMW)ab-sorption.Herein,a novel bi-orientation etching approach is proposed to regulate the chemical composi-tions and hybridization construction of Zn/Co hybrids,thence meeting the synergistic interaction among diverse compositions for ultra-high EMW absorption.Attributing to the stepwise heterometallic modi-fication by Zn in Co-ZIFs(Zn@Co-ZIF)and Co in Zn-ZIFs(Co@Zn-ZIF),corresponding pyrolysis products with variable Zn/Co atomic concentration and hybridization states(Zn@Co-C,Co@Zn-C)can be obtained.The Co species was confirmed to have a positive impact on both the dielectric and magnetic response,while an appropriate amount of Zn hybridization in Co substance further enhanced the synergistic ef-fect between multiple loss mechanisms,promoting the minimum reflection loss and effective absorption bandwidth of Zn@Co-C reached-34.99 dB and 5.52 GHz(2.00 mm),respectively.Finally,this work re-veals the composition and construction dependency of bimetal hybrids on EMW absorption,which can be a strong reference for developing EMAs with diverse hybridization structures. | Xiuyun Ren Yihe Song Zhenguo Gao Yalan Wu Zirui Jia Guanglei Wu | 2023 | Journal of Materials Science & Technology2023，，3： | 1 |
| 7 | Characterization of phosphate monoester resistant starch 显示文摘 | Guanglei Li JieZeng Haiyan Gao | 2011 | International Journal of Food Properties2011，14，： | 1 |
| 8 | Individual tree simulation and stand visualization of Pinus tabulaeformis plantation显示文摘 | Gao Guanglei Ding Guodong Zang Yintong | 2011 | International Journal of Emerging Sciences2011，1，4： | 1 |
| 9 | Fractal scaling of particle size distribution and relationships with topsoil properties affected by biological soil crusts显示文摘 | Gao Guanglei Ding Guodong Wu Bin | 2014 | PLoS ONE2014，9，88： | 1 |
| 10 | Size-and leaf age-dependent effects on the photosynthetic and physiological responses of Artemisia ordosica to drought stress显示文摘Drought is one of the most significant natural disasters in the arid and semi-arid areas of China.Populations or plant organs often differ in their responses to drought and other adversities at different growth stages.At present,little is known about the size-and leaf age-dependent differences in the mechanisms of shrub-related drought resistance in the deserts of China.Here,we evaluated the photosynthetic and physiological responses of Artemisia ordosica Krasch.to drought stress using a field experiment in Mu Us Sandy Land,Ningxia Hui Autonomous Region,China in 2018.Rainfall was manipulated by installing outdoor shelters,with four rainfall treatments applied to 12 plots(5 m×5 m).There were four rainfall levels,including a control and rainfall reductions of 30%,50%and 70%,each with three replications.Taking individual crown size as the dividing basis,we measured the responses of A.ordosica photosynthetic and physiological responses to drought at different growth stages,i.e.,large-sized(>0.5 m^(2))and small-sized(≤0.5 m^(2))plants.The leaves of A.ordosica were divided into old leaves and young leaves for separate measurement.Results showed that:(1)under drought stress,the transfer efficiency of light energy captured by antenna pigments to the photosystem II(PSII)reaction center decreased,and the heat dissipation capacity increased simultaneously.To resist the photosynthetic system damage caused by drought,A.ordosica enhanced its free radical scavenging capacity by activating its antioxidant enzyme system;and(2)growth stage and leaf age had effects on the reaction of the photosynthetic system to drought.Small A.ordosica plants could not withstand severe drought stress(70%rainfall reduction),whereas large A.ordosica individuals could absorb deep soil water to ensure their survival in severe drought stressed condition.Under 30%and 50%rainfall reduction conditions,young leaves had a greater ability to resist drought than old leaves,whereas the latter were more resistant to severe drought stress.The response of A.ordosica photosynthetic system reflected the trade-off at different growth stages and leaf ages of photosynthetic production under different degrees of drought.This study provides a more comprehensive and systematic perspective for understanding the drought resistance mechanisms of desert plants. | WANG Chunyuan YU Minghan DING Guodong GAO Guanglei ZHANG Linlin HE Yingying LIU Wei | 2021 | Journal of Arid Land2021，13，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员