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| 1 | Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)显示文摘Passion fruit(Passiflora edulis Sims)is an economically valuable fruit that is cultivated in tropical and subtropical regions of the world.Here,we report an~1341.7Mb chromosome-scale genome assembly of passion fruit,with 98.91%(~1327.18Mb)of the assembly assigned to nine pseudochromosomes.The genome includes 23,171 protein-coding genes,and most of the assembled sequences are repetitive sequences,with long-terminal repeats(LTRs)being the most abundant.Phylogenetic analysis revealed that passion fruit diverged after Brassicaceae and before Euphorbiaceae.Ks analysis showed that two whole-genome duplication events occurred in passion fruit at 65 MYA and 12 MYA,which may have contributed to its large genome size.An integrated analysis of genomic,transcriptomic,and metabolomic data showed that‘alpha-linolenic acid metabolism’,‘metabolic pathways’,and‘secondary metabolic pathways’were the main pathways involved in the synthesis of important volatile organic compounds(VOCs)in passion fruit,and this analysis identified some candidate genes,including GDP-fucose Transporter 1-like,Tetratricopeptide repeat protein 33,protein NETWORKED 4B isoform X1,and Golgin Subfamily A member 6-like protein 22.In addition,we identified 13 important gene families in fatty acid pathways and eight important gene families in terpene pathways.Gene family analysis showed that the ACX,ADH,ALDH,and HPL gene families,especially ACX13/14/15/20,ADH13/26/33,ALDH1/4/21,and HPL4/6,were the key genes for ester synthesis,while the TPS gene family,especially PeTPS2/3/4/24,was the key gene family for terpene synthesis.This work provides insights into genome evolution and flavor trait biology and offers valuable resources for the improved cultivation of passion fruit. | Zhiqiang Xia Dongmei Huang Shengkui Zhang Wenquan Wang Funing Ma Bin Wu Yi Xu Bingqiang Xu Di Chen Meiling Zou Huanyu Xu Xincheng Zhou Rulin Zhan Shun Song | 2021 | Horticulture Research2021，8，1： | 15 |
| 2 | Efficient extraction and stripping of Nd(Ⅲ),Eu(Ⅲ) and Er(Ⅲ) by membrane dispersion micro-extractors显示文摘The extraction of low concentration rare earth elements at high phase ratio was investigated. The traditional extraction set-up, such as mixer-settler, have drawbacks of easy emulsification, difficult separation and low efficiency if operated at the above condition. Membrane dispersion micro-extractor,owing to its well-dispersed, high surface-to-volume ratio and fast mass transfer rate, was employed in our work. Nd(Ⅲ),Eu(Ⅲ),Er(Ⅲ) were chosen to represent light, medium,heavy rare earth elements(REEs). The extraction process of REEs with 2-ethylhexyl phosphoric acid-2-ethylhexyl ester(P507) was investigated by membrane dispersion micro-extractors. Firstly, the extraction equilibrium of these three elements was explored in the stirred conical flasks, and it is indicated that the extraction efficiencies can be 0.95, 0.97 and 0.98, respectively within 40 min at phase ratio of 100:1. Then the effects of operational conditions such as the residence time, organic and aqueous flow rates on extraction efficiency were also explored in micro-extractors. The results indicate that the efficiency decreases and then increases if increasing aqueous phase flow rate, residence time and droplets' diameter are the key factors of this process. Increasing the phase ratio reduces the extraction efficiency significantly. When the REEs solution has an initial pH of 4.00, the flow rates of continuous and dispersed phase are 40 and 1.6 mL/min,respectively, and 90 mg/L Nd(Ⅲ), Eu(Ⅲ) and Er(Ⅲ) is extracted by 1 mol/L P507 at the out-let length of8 m. The extraction efficiencies are 0.978,0.983 and 0.991, respectively. Finally the stripping process was also studied with the micro-extractor. The stripping efficiencies of Nd(Ⅲ), Eu(Ⅲ) and Er(Ⅲ) can reach0.99, 0.96 and 0.91, respectively when the out-let length is 8 m and the concentration of hydrochloric acid is 1 mol/L. The developed approach offers a novel and simple strategy on the fast extraction and enrichment of low concentration rare earth elements from waste water. | Zhuo Chen Jianhong Xu Funing Sang Yundong Wang | 2018 | Journal of Rare Earths2018，36，8： | 2 |
| 3 | Involvement of the PI3K/Akt signal pathway in the hypoglycemic effects of tea polysaccharides on diabetic mice显示文摘 | Shuqin Li Haixia Chen Jia Wang Xiuming Wang Bo Hu Funing Lv | 2015 | International Journal of Biological Macromolecule2015，，： | 1 |
| 4 | Mitoehondri- al-and Fas-L-mediated pathways involved in quinestrol in- duced spermatogenic apoptosis in adult rat testes显示文摘 | LI Jian CHEN Funing CHEN Yaoxing | 2014 | Toxi- eology mechanisms and methods2014，24，9： | 1 |
| 5 | Efficient nanozyme engineering for antibacterial therapy显示文摘Antimicrobial resistance(AMR)poses a huge threat to human health.It is urgent to explore efficient ways to suppress the spread of AMR.Antibacterial nanozymes have become one of the powerful weapons to combat AMR due to their enzyme-like catalytic activity with a broad-spectrum antibacterial performance.However,the inherent low catalytic activity of nanozymes limits their expansion into antibacterial applications.In this regard,a variety of advanced chemical design strategies have been developed to improve the antimicrobial activity of nanozymes.In this review,we have summarized the recent progress of advanced strategies to engineer efficient nanozymes for fighting against AMR,which can be mainly classified as catalytic activity improvement,external stimuli,bacterial affinity enhancement,and multifunctional platform construction according to the basic principles of engineering efficient nanocatalysts and the mechanism of nanozyme catalysis.Moreover,the deep insights into the effects of these enhancing strategies on the nanozyme structures and properties are highlighted.Finally,current challenges and future perspectives of antibacterial nanozymes are discussed for their future clinical potential. | Yonghai Feng Funing Chen Jessica M Rosenholm Lei Liu Hongbo Zhang | 2022 | Materials Futures2022，1，2： | 0 |
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