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| 1 | Transportation characteristics of δ^(13)C in the plants-soil-bedrock-cave system in Chongqing karst area显示文摘Here we use an analytical method to determine δ13C in local plants and organic matter in the soils above Furong cave,Chongqing,China.We also monitored δ13C in dissolved inorganic carbon(DIC) of drip water,δ13C of active deposits under the drip waters,and the concentration of air CO2(PCO2).Based on these,we preliminarily studied the transportation characteristics of stable carbon isotope(13C) in cave system of the subtropical karst area.The average δ13C value of 27 local plant samples,which belong to 16 families,was -32‰ and the weighted δ13C for surface dry biomass was -33‰.We found that for 54 soil samples collected from 5 soil profiles,δ13C of soil organic matters was -22‰,which could be attributed to the different transportation rates of stable carbon isotopes during the decomposition of plants and organic matters in soils.The relatively lighter 12C tended to transfer into gaseous CO2,which made the relatively heavier 13C concentrated in the soils.On the basis of monitoring of DIC-δ13C in drip waters from July 2009 to June 2010,we found that values in winter months were heavier and values in summer months were lighter in general,the reason of which was that in summer months,both the temperature and the humidity were comparatively higher,resulted in more CO2 with lighter δ13C generated from organic matters decomposition and plants respiration.The average DIC -δ13C value was -11‰,about 11‰ heavier than the δ13C of organic matters in soils,which proved that part of DIC in cave drip water was sourced from dissolution of inorganic carbonate(host rock,with heavier δ13C).As for the δ13C of active deposits at five drip water sites in Furong cave,they had almost the same variation with relatively light values.In other words,these active speleothems were deposited at equilibrium conditions for isotopic fractionation.These results suggest that the carbon isotopic information of speleothems could be used to track the evolution of local vegetation in certain situations. | Li TingYong Li HongChun Xiang XiaoJing Kuo Tz-Shing Li JunYun Zhou FuLi Chen HongLi Peng LingLi | 2012 | Science China Earth Sciences2012，55，4： | 18 |
| 2 | North American ginseng protects the heart from ischemia and reperfusion injury via upregulation of endothelial nitric oxide synthase显示文摘 | Yan Wu Xiangru Lu Fuli Xiang et o1 | 2011 | Pharma- cological research2011，64，3： | 1 |
| 3 | Development of an Event-specific Quantitative PCR for Genetically Modified Maize (Zea mays) Event NK603显示文摘[Objective] The aim of this study was to develop a quantitative PCR detection method for genetically modified maize event NK603, so as to provide basis for quantitative analysis of event NK603. [Methods] A quantitative PCR detection method for genetically modified maize event NK603 was developed using primers and Taqman probe designed according to the flanking sequence of event NK603, which was then adopted to detect the samples containing 2% NK603 standard (with uncertain quantity of 10%). [Results] The slope of standard curve ranged between -3.6 and -3.1, and the correlation coefficient was higher than 0.99. The amplification efficiency of this method reached 100.2%, fallen between 90% and 110%. The detected quantity of the experimental sample was 1.9%, closer to the true quantity (2%). [Conclusion] This quantitative PCR detection method for genetically modified maize event NK603 is very precise and can be adopted in routine testing analysis. | Jun SONG Shaorong LEI Yong LIU Quan YIN Dong WANG Bing XIANG Fuli ZHANG Wenjuan LIU Lijuan CHANG | 2012 | Agricultural Biotechnology2012，1，5： | 0 |
| 4 | Jujube witches’ broom phytoplasmas inhibit ZjBRC1-mediated abscisic acid metabolism to induce shoot proliferation显示文摘Jujube witches’broom(JWB)phytoplasmas parasitize the sieve tubes of diseased phloem and cause an excessive proliferation of axillary shoots from dormant lateral buds to favour their transmission.In previous research,two JWB effectors,SJP1 and SJP2,were identified to induce lateral bud outgrowth by disrupting ZjBRC1-mediated auxin flux.However,the pathogenesis of JWB disease remains largely unknown.Here,tissue-specific transcriptional reprogramming was examined to gain insight into the genetic mechanisms acting inside jujube lateral buds under JWB phytoplasma infection.JWB phytoplasmas modulated a series of plant signalling networks involved in lateral bud development and defence,including auxin,abscisic acid(ABA),ethylene,jasmonic acid,and salicylic acid.JWB-induced bud outgrowth was accompanied by downregulation of ABA synthesis within lateral buds.ABA application rescued the bushy appearances of transgenic Arabidopsis overexpressing SJP1 and SJP2 in Col-0 and ZjBRC1 in the brc1-2 mutant.Furthermore,the expression of ZjBRC1 and ABA-related genes ZjHB40 and ZjNCED3 was negatively correlated with lateral main bud outgrowth in decapitated healthy jujube.Molecular evidence showed that ZjBRC1 interacted with ZjBRC2 via its N-terminus to activate ZjHB40 and ZjNCED3 expression and ABA accumulation in transgenic jujube calli.In addition,ZjBRC1 widely regulated differentially expressed genes related to ABA homeostasis and ABA signalling,especially by binding to and suppressing ABA receptors.Therefore,these results suggest that JWB phytoplasmas hijack the ZjBRC1-mediated ABA pathways to stimulate lateral bud outgrowth and expansion,providing a strategy to engineer plants resistant to JWB phytoplasma disease and regulate woody plant architecture to promote crop yield and quality. | Fuli Ma Shanqi Zhang Yu Yao Mengting Chen Ning Zhang Mingsheng Deng Wei Chen Chi Ma Xinyue Zhang Chenglong Guo Xiang Huang Zhenyuan Zhang Yamei Li Tingyi Li Junyong Zhou Qibao Sun Jun Sun | 2023 | Horticulture Research2023，10，9： | 0 |
| 5 | Uncertainty in Detecting Specific Fragment of Genetically Modified Maize Event NK603 by Real-time Fluorescence Quantitative PCR显示文摘In this study,the event-specific real-time fluorescence quantitative PCR method established by Sichuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment( specific fragment) in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources,such as PCR amplification system,data analysis and micropipette. The results showed that A-type uncertainty( uA),B-type uncertainty( uB),combined standard uncertainty( uC) and expanded uncertainty( U95) were0.000 8,0.001,0.001 and0.002,respectively; the final detection result was 1. 9% ± 0. 002. Thus,the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental | Jun SONG Shaorong LEI Yong LIU Dong WANG Wenjuan LIU Quan YIN Bing XIANG Fuli ZHANG Lijuan CHANG | 2014 | Agricultural Biotechnology2014，3，4： | 0 |
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