3篇
您的检索式：作者名="Fubing Shen"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Anti-tumor activity and immunological modification of ribosome-inactivating protein (RIP) from Momordica charantia by covalent attachment of polyethylene glycol显示文摘使核糖体失去活性的蛋白质(裂开) 是酶的一个家庭那 depurinate rRNA 并且禁止蛋白质生合成。这里,我们报导纯化,导致 apoptosis 活动,和聚乙烯乙二醇(木钉) 修正从痛苦的甜瓜种子裂开。蛋白质有 N-Asp-Val-Ser-Phe-Arg 的一个同质的 N 终端序列。而且, RIP 显示了强壮的导致 apoptosis 活动和压制的癌症细胞生长。这可能被归因于 caspases-3 的激活。使它可得到为在 vivo 申请, immunogenicity 裂开被化学修正与 20 kDa (mPEG ) 减少 2-Lys-NHS。PEGylated 和 non-PEGylated 的抑制活动对癌症房间裂开比对正常 PEGylated 的房间,和 antigenicity 裂开的强壮得多显著地被减少。我们的结果建议 PEGylated 裂开可能潜在地作为反癌症被开发药。 | Mengen Li Yiwen Chen Zhongyu Liu Fubing Shen Xiaoxiao Bian Yanfa Meng | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 11 |
| 2 | Acetylcholinesterase liquid crystal biosensor for identification of AChE inhibitors by a reactivator显示文摘An improved acetylcholinesterase liquid crystal(LC) biosensor has been developed for the identification of organophosphates(OPs) by using a reactivator. When the acetylcholinesterases(AChEs) inhibited by different kinds of OPs are reactived by a reactivator, the catalytic activity of AChEs can be recovered with different activation efficiency because of the different phosphorylation structures formed in the inhibited AChEs. Accordingly, the reactived AChEs can catalyze the hydrolysis of acetylthiocholine to generate thiocholine product in different degrees, which will result in different catalytic growth of AuNPs and further form distinct orientational response of LCs. Based on such a reactivation mechanism, the AChE LC biosensor with a simple, rapid and visual procedure achieves an obvious identification of three OPs pesticides, methamidophos, trichlorfon and paraoxon, by using a pralidoxime reactivator. | DING HuaZhi LIAO ShuZhen XIAO FuBing SHEN GuoLi YU RuQin WU ZhaoYang | 2014 | Science China Chemistry2014，57，11： | 1 |
| 3 | Touchable cell biophysics property recognition platforms enable multifunctional blood smart health care显示文摘As a crucial biophysical property,red blood cell(RBC)deformability is pathologically altered in numerous disease states,and biochemical and structural changes occur over time in stored samples of otherwise normal RBCs.However,there is still a gap in applying it further to point-of-care blood devices due to the large external equipment(high-resolution microscope and microfluidic pump),associated operational difficulties,and professional analysis.Herein,we revolutionarily propose a smart optofluidic system to provide a differential diagnosis for blood testing via precise cell biophysics property recognition both mechanically and morphologically.Deformation of the RBC population is caused by pressing the hydrogel via an integrated mechanical transfer device.The biophysical properties of the cell population are obtained by the designed smartphone algorithm.Artificial intelligence-based modeling of cell biophysics properties related to blood diseases and quality was developed for online testing.We currently achieve 100%diagnostic accuracy for five typical clinical blood diseases(90 megaloblastic anemia,78 myelofibrosis,84 iron deficiency anemia,48 thrombotic thrombocytopenic purpura,and 48 thalassemias)via real-world prospective implementation;furthermore,personalized blood quality(for transfusion in cardiac surgery)monitoring is achieved with an accuracy of 96.9%.This work suggests a potential basis for next-generation blood smart health care devices. | Longfei Chen Yantong Liu Hongshan Xu Linlu Ma Yifan Wang Le Yu Fang Wang Jiaomeng Zhu Xuejia Hu Kezhen Yi Yi Yang Hui Shen Fuling Zhou Xiaoqi Gao Yanxiang Cheng Long Bai Yongwei Duan Fubing Wang Yimin Zhu | 2021 | Microsystems & Nanoengineering2021，7，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员