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| 1 | Hepatectomy with primary closure of common bile duct for hepatolithiasis combined with choledocholithiasis显示文摘AIM: To evaluate the feasibility of hepatectomy and primary closure of common bile duct for intrahepatic and extrahepatic calculi. METHODS: From January 2008 to May 2013, anatomic hepatectomy followed by biliary tract exploration without biliary drainage(non-drainage group) was performed in 43 patients with intrahepatic and extrahepatic calculi. After hepatectomy, flexible choledochoscopy was used to extract residual stones and observe the intrahepatic bile duct and common bile duct(CBD) for determination of biliary stricture and dilatation. Function of the sphincter of Oddi was determined by manometry of the CBD. Primary closure of the CBD without T-tube drainage or bilioenteric anastomosis was performed when there was no biliary stricture or sphincter of Oddi dysfunction. Dexamethasone and anisodamine were intravenously injected 2-3 d after surgery to prevent postoperative retrograde infection due to intraoperative bile duct irrigation, and to maintain relaxation of the sphincter of Oddi, respectively. During the same period, anatomic hepatectomy followed by biliary tract exploration with biliary drainage(drainage group) was performed in 48 patients as the control group. Postoperative complications and hospital stay were compared between the two groups.RESULTS: There was no operative mortality in either group of patients. Compared to intrahepatic and extrabiliary drainage, hepatectomy with primary closure of the CBD(non-drainage) did not increase the incidenceof complications, including residual stones, bile leakage, pancreatitis and cholangitis(P > 0.05). Postoperative hospital stay and costs were nevertheless significantly less in the non-drainage group than in the drainage group. The median postoperative hospital stay was shorter in the non-drainage group than in the drainage group(11.2 ± 2.8 d vs 15.4 ± 2.1 d, P = 0.000). The average postoperative cost of treatment was lower in the non-drainage group than in the drainage group(29325.6 ± 5668.2 yuan vs 32933.3 ± 6235.1 yuan, P = 0.005). CONCLUSION: Hepatectomy followed by choledochoendoscopic stone extraction without biliary drainage is a safe and effective treatment of hepatolithiasis combined with choledocholithiasis. | Chang-Ku Jia Jie Weng You-Ke Chen Qing-Zhuang Yang Yu Fu Qi-Fan Qin Wei-Ming Yu | 2015 | World Journal of Gastroenterology2015，21，12： | 27 |
| 2 | Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens显示文摘The study of pathophysiological mecha nisms in huma n liver disease has bee n constrained by the in ability to expa nd primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be con verted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD +-dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded sign ifica ntly duri ng in vitro passage. The expa nded cells can readily be con verted back into metabolically functional hepatocytes in vitro and upon tran splantati on in vivo. Under three-dime nsional culture conditions, differentiated cells gen erated from HepLPCs regai ned the ability to support infect io n or reactivatio n of hepatitis B virus (HBV). Our work dem on strates the utility of the con versi on betwee n hepatocyte and liver proge nitor-like cells for studying HBV biology and an tiviral therapies. These fin dings will facilitate the study of liver diseases and regenerative medicine. | Gong-Bo Fu Wei-Jian Huang Min Zeng Xu Zhou Hong-Ping Wu Chang-Cheng Liu Han Wu Jun Weng Hong-Dan Zhang Yong-Chao Cai Charles Ashton Min Ding Dan Tang Bao-Hua Zhang Yi Gao Wei-Feng Yu Bo Zhai Zhi-Ying He Hong-Yang Wang He-Xin Yan | 2019 | Cell Research2019，29，1： | 18 |
| 3 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 4 | Anatomic resection of liver segments 6-8 for hepatocellular carcinoma显示文摘AIM:To report the devised anatomic liver resection of segments 6,7 and 8 to improve the resection rate for patients with right liver tumors.METHODS:We performed anatomic liver resection of segments 6,7 and 8 to guarantee the maximum preservation of the remaining normal liver tissue.Segment 5 was determined by two steps of Glissonean pedicle occlusion.And a'┏┛'shaped broken resection line was marked upon the diaphragmatic surface of the liver.Selective right hemihepatic inflow occlusion was used to reduce blood loss during parenchymal transection between segments 6 and 5 and between segments 8 and 5.If needed,total hepatic Glissonean pedicle occlusion was used during parenchymal transection between segment 8 and the left liver.RESULTS:Compared to right hemihepatectomy,the percentage of future liver remnant volume was increased by an average of 13.9%if resection of segments 6,7 and 8 was performed.Resection of segments 6,7 and 8 was completed uneventfully.After hepatectomy,the inflow and outflow of segment 5were maintained.There was no perioperative mortality,postoperative abdominal bleeding or bile leakage in this group.Alpha-fetoprotein(AFP)returned to the normal range within 2 mo after the operation in all the patients.One patient died 383 d postoperatively due to obstructive suppurative cholangitis.One patient suffered from severe liver dysfunction shortly after surgery and had intrahepatic recurrence 4 mo postoperatively.Postoperative lung metastasis was found in one patient.No tumor recurrence was found in the other patients and the parameters including liver function and AFP level were in the normal range.CONCLUSION:Anatomic liver resection of segments6,7 and 8 can be a conventional operation to improve the overall resection rate for hepatocellular carcinoma. | Chang-Ku Jia Jie Weng You-Ke Chen Yu Fu | 2014 | World Journal of Gastroenterology2014，20，15： | 8 |
| 5 | Lung-protective Ventilation in Patients with Brain Injury: A Multicenter Cross-sectional Study and Questionnaire Survey in China显示文摘 | Xu-Ying Luo Ying-Hong Hu Xiang-Yuan Cao Yan Kang Li-Ping Liu Shou-Hong Wang Rong-Guo Yu Xiang-You Yu Xia Zhang Bao-Shan Li Zeng-Xiang Ma Yi-Bing Weng Heng Zhang De-Chang Chen Wei Chen Wen-Jin Chen Xiu-Mei Chen Bin Du Mei-Li Duan Jin Hu Yun-Feng Hoang Gui-Jun Jia Li-Hong Li Yu-Min Liang Bing-Yu Qin Xian-Dong Wang Jian Xiong Li-Mei Yan Zheng-Ping Yang Chen-Ming Dong Dong-Xin Wang Qing-Yuan Zhan Shuang-Lin FU Lin Zhao Qi-Bing Huang Ying-Guang Xie Xiao-Bo Huang Guo-Bin Zhang Wang-Bin Xu Yuan Xu YaLing Liu He-Ling Zhao Rong-Qing Sun Ming Sun Qing-Hong Cheng Xin Qu Xiao-Feng Yang Ming Xu Zhong-Hua Shi Han Chen Xuan He Yan-Lin Yang Guang-Qiang Chen Xiu-Mei Sun Jian-Xin Zhou | 2016 | Chinese Medical Journal2016，，14： | 6 |
| 6 | In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells显示文摘Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have revealed that the circadian rhythm may disrupt the growth of tumors,it is generally unclear whether the circadian rhythm controls the release of CTCs.In clinical examinations,the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time,which is being challenged by recent studies.Since it is not practical to draw blood from patients repeatedly,a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods.Fluoresce nee in vivo flow cytometry(IVFC)is a powerful optical technique that is able to detect fluoresce nt circulating cells directly in living animals in a noninvasive manner over a long period of time.In this study,we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer.We observed that CTCs exhibited stochastic bursts over cancer progression.The probability of the bursting activity was higher at early stages than at late stages.We longitudinally monitored CTCs over a 24-h period,and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night(active phase for rodents),suggesting that the release of CTCs might be regulated by the circadian rhythm. | Xi Zhu Yuanzhen Suo Yuting Fu Fuli Zhang Nan Ding Kai Pang Chengying Xie Xiaofu Weng Meilu Tian Hao He Xunbin Wei | 2021 | Light(Science & Applications)2021，10，6： | 6 |
| 7 | Advances in graphene reinforced metal matrix nanocomposites:Mechanisms,processing,modelling,properties and applications显示文摘Graphene has been extensively explored to enhance functional and mechanical properties of metalmatrix nanocomposites for wide-range applications due to their superior mechanical,electrical and thermal properties.This article discusses recent advances of key mechanisms,synthesis,manufacture,modelling and applications of graphene metal matrix nanocomposites.The main strengthening mechanisms include load transfer,Orowan cycle,thermal mismatch,and refinement strengthening.Synthesis technologies are discussed including some conventional methods(such as liquid metallurgy,powdermetallurgy,thermal spraying and deposition technology)and some advanced processing methods(such as molecular-level mixing and friction stir processing).Analytical modelling(including phenomenological models,semi-empirical models,homogenization models,and self-consistent model)and numerical simulations(including finite elements method,finite difference method,and boundary element method)have been discussed for understanding the interface bonding and performance characteristics between graphene and different metal matrices(Al,Cu,Mg,Ni).Key challenges in applying graphene as a reinforcing component for the metal matrix composites and the potential solutions as well as prospectives of future development and opportunities are highlighted. | Wenge Chen Tao Yang Longlong Dong Ahmed Elmasry Jiulong Song Nan Deng Ahmed Elmarakbi Terence Liu Hai Bao Lv Yong Qing Fu | 2020 | Nanotechnology and Precision Engineering2020，3，4： | 4 |
| 8 | Short-term link quality prediction using nonparametric time series analysis显示文摘Wireless link quality prediction(LQP) is the foundation for proactive operations and is therefore a key technique in alleviating network performance degradation. However, accurate LQP is difficult because of the dynamic nature of wireless environments. A recent study found that fluctuations in intermediate quality links often show dynamics on a sub-second granularity, making the task even more challenging. In order to leverage the intermediate links, as well as fine-tune upper-layer protocols, we propose to use nonparametric modeling in nonlinear time series analysis that predicts short-term link quality online. Unlike existing studies,we do not define any new experimental or hypothetical models, or train models using a set of training data.Functional-coefficient autoregression is employed to predict the link dynamics at high time resolutions. We apply our approach and a local linear regression-based LQP(a typical parametric modeling approach) to both NS-2 simulation and empirical packet traces. The results indicate that the proposed method has much higher prediction accuracy and convergence speed than the local linear regression-based LQP under dynamic network conditions. | WENG LiNa ZHANG Ping FENG ZhiYong CHENG HongWei LIAN Hao FU Bin | 2015 | Science China(Information Sciences)2015，58，8： | 4 |
| 9 | Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity显示文摘The developing trends of livestock production are efficiency,safety and sustainability,which face two major challenges:low availability of phytate phosphorus and abuse of antibiotics.As a solution phytases and antimicrobial peptides are applied as feed additives.However,phytases and antimicrobial peptides are susceptible to proteases,costly by fermentation and potential toxic to production hosts.We transformed an optimized phytase-lactoferricin fusion gene PhyLf driven by an endosperm-specific promoter Gt13aP and Bar(bialaphos resistance) gene as a selection maker into rice.The Bar and PhyLf genes were integrated into the rice genome,stably inherited and expressed.Their phosphinothricin acetyl transferase(PAT)protein content oftransgenic plants with glufosinate resistance varied between 50.45-93.39 ugg^(-1).Fusion protein expressed especially in the seeds oftransgenic rice had a summit phytase activity at 32.30Ug^(-1) which increased by 61.71-fold compared to the control/check group(CK) and 7.54-fold compared to un-optimized transgenic plant.The highest inorganic phosphorus(Pi) content of the transgenic seeds reached 13.15 mg g^(-1) increased by 12.77-fold compared to that of CK.Preliminary antibacterial experiments showed that the enterokinase hydrolysate product of fusion protein could inhibit the growth of Escherichia coli DH5a.These results indicated that the protein PhyLf has the potential to increase availability of feed phytate phosphorus,improve consumer's immunity and reduce the use of antibiotics. | WANG Zuo-ping DENG Li-hua WENG Lü-shui DENG Xiang-yang FU Xi-qin XIN Ye-yun XIAO Guo-ying | 2017 | Journal of Integrative Agriculture2017，16，4： | 4 |
| 10 | Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer’s disease mouse model显示文摘Photobiomodulation,by utilizing low-power light in the visible and near-infrared spectra to trigger biological responses in cells and tissues,has been considered as a possible therapeutic strategy for Alzheimer’s disease(AD),while its specific mechanisms have remained elusive.Here,we demonstrate that cognitive and memory impairment in an AD mouse model can be ameliorated by 1070-nm light via reducing cerebralβ-amyloid(Aβ)burden,the hallmark of AD.The glial cells,including microglia and astrocytes,play important roles in Aβclearance.Our results show that 1070-nm light pulsed at 10 Hz triggers microglia rather than astrocyte responses in AD mice.The 1070-nm lightinduced microglia responses with alteration in morphology and increased colocalization with Aβare sufficient to reduce Aβload in AD mice.Moreover,1070-nm light pulsed at 10 Hz can reduce perivascular microglia and promote angiogenesis to further enhance Aβclearance.Our study confirms the important roles of microglia and cerebral vessels in the use of 1070-nm light for the treatment of AD mice and provides a framework for developing a novel therapeutic approach for AD. | Lechan Tao Qi Liu Fuli Zhang Yuting Fu Xi Zhu Xiaofu Weng Hongbin Han Yong Huang Yuanzhen Suo Liang Chen Xiaoling Gao Xunbin Wei | 2021 | Light(Science & Applications)2021，10，10： | 4 |
| 11 | Classification of brain arteriovenous malformations located in motor-related areas based on location and anterior choroidal artery feeding显示文摘Objective Surgical management of arteriovenous malformations(AVMs)involving motor cortex or fibre tracts(M-AVMs)is challenging.This study aimed to construct a classification system based on nidus locations and anterior choroidal artery(AChA)feeding to pre-surgically evaluate motor-related and seizure-related outcomes in patients undergoing resection of M-AVMs.Methods and materials A total of 125 patients who underwent microsurgical resection of M-AVMs were retrospectively reviewed.Four subtypes were identified based on nidus location:(I)nidus involving the premotor area and/or supplementary motor areas;(II)nidus involving the precentral gyrus;(III)nidus involving the corticospinal tract(CST)and superior to the posterior limb of the internal capsule;(IV)nidus involving the CST at or inferior to the level of posterior limb of the internal capsule.In addition,we divided type IV into type IVa and type IVb according to the AChA feeding.Surgical-related motor deficit(MD)evaluations were performed 1 week(short-term)and 6 months(long-term)after surgery.Results The type I patients exhibited the highest incidence(62.0%)of pre-surgical epilepsy among the four subtypes.Multivariate analysis showed that motor-related area subtypes(p=0.004)and diffuse nidus(p=0.014)were significantly associated with long-term MDs.Long-term MDs were significantly less frequent in type I than in the other types.Type IV patients acquired the highest proportion(four patients,25.0%)of long-term poor outcomes(mRS>2).Type IVb patients showed a significantly higher incidence of post-surgical MDs than type IVa patients(p=0.041).The MDs of type III or IV patients required more recovery time.Of the 62 patients who had pre-surgical seizures,90.3%(56/62)controlled their seizures well and reached Engel class I after surgery.Conclusions Combining the consideration of location and AChA feeding,the classification for M-AVMs is a useful approach for predicting post-surgical motor function and decision-making. | Yuming Jiao Hao Li Weilun Fu Jiancong Weng Ran Huo Yinyan Wang Shuo Wang Tao Jiang Yong Cao Ji Zong Zhao | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 12 | Extension Protocol for the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC)Study:2021 update显示文摘Background Malnutrition is common in patients with cancer,and this adversely affects the survival and quality of life of patients.Chinese Society for Nutritional Oncology issued a multi-center,large-scale,long-term follow-up prospective study,the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC study)since 2013.This is an extension to the previous 2013-2020 study protocol.This study still sought to:①address the prognostic impact of nutritional factors and quality of life on cancer patient survival;②describe the overall and cancer-specific incidence and/or distribution of malnutrition and different measurements of patient quality of life.Methods and study design This is an observational,multi-centered,hospital-based prospective cohort study.Data collection will be performed at baseline(within 48 hours after patient admission),during the hospital stay and 30 days after hospital admission.Follow-up will be conducted for 1-20 years after enrollment.The primary outcome will be the all-cause mortality/overall survival,and secondary outcomes will be the length of hospital stay and costs of hospitalization.Study factors will include demographic characteristics,tumor characteristics,information about chronic diseases,hematological measurements(e.g.,red blood cell count,total lymphocyte counts,hemoglobin,albumin,prealbumin,creatinine,C-reactive protein,IL-6),anthropometric measurements(e.g.,height,weight,arm circumference,arm muscle circumference,triceps skinfold thickness,and waist circumference),body composition parameters,PG-SGA scores,quality of life(as indicated by the QLQ-C30 questionnaire),muscle mass(as indicated by the calf circumference),muscle strength(as indicated by the handgrip strength),muscle function(as indicated by the six-meter walking speed test)and physical status assessments(as indicated by the Karnofsky Performance Status scores).This clinical study protocol was approved by local Ethics Committees of all the participating hospitals.Written informed consent is required for each subject included.Discussion This multi-center,large-scale,long-term follow up prospective study will help improve the diagnosis of malnutrition in cancer patients and identify the risk factors associated with adverse clinical outcomes.The anticipated results of this study will highlight the need for a truly scientific appraisal of nutrition therapy in Chinese oncology populations,and finally help treat the potentially reversible elements of malnutrition in cancer patients to improve their clinical outcomes in the future. | Hong Xia Xu Chun Hua Song Liang Yu Yin Chang Wang Zhen Ming Fu Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Zhi Kang Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Xiang Hua Wu Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Ya Yin Yu Quan Jun Lyu Hong Qiu Ming Liu Wei Li Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2022 | Journal of Nutritional Oncology2022，7，2： | 3 |
| 13 | Reply to Comment on “In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells”显示文摘Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry in a multiple myeloma mouse model in their previous work2 might be different from our results.Niedre et al.claimed that CTC detection statistics deviated from Poisson but did not found circadian variations in CTC numbers in a multiple myeloma mouse model.They also cite another literature by Juratli.et al.3,in which the authors reported that CTC numbers did not always correlate with tumor size during cancer progression.However,by establishing an orthotopic mouse model of prostate cancer and utilizing the technology in vivo flow cytometry(IVFC),we found CTCs exhibited bursting activity and daily oscillation in an orthotopic model of prostate cancer. | Xi Zhu Yuanzhen Suo Yuting Fu Fuli Zhang Nan Ding Kai Pang Chengying Xie Xiaofu Weng Meilu Tian Hao He Xunbin Wei | 2021 | Light(Science & Applications)2021，10，10： | 2 |
| 14 | Application of imputation methods to genomic selection in Chinese Holstein cattle显示文摘Missing genotypes are a common feature of high density SNP datasets obtained using SNP chip technology and this is likely to decrease the accuracy of genomic selection. This problem can be circumvented by imputing the missing genotypes with estimated genotypes. When implementing imputation, the criteria used for SNP data quality control and whether to perform imputation before or after data quality control need to consider. In this paper, we compared six strategies of imputation and quality control using different imputation methods, different quality control criteria and by changing the order of imputation and quality control, against a real dataset of milk production traits in Chinese Holstein cattle. The results demonstrated that, no matter what imputation method and quality control criteria were used, strategies with imputation before quality control performed better than strategies with imputation after quality control in terms of accuracy of genomic selection. The different imputation methods and quality control criteria did not significantly influence the accuracy of genomic selection. We concluded that performing imputation before quality control could increase the accuracy of genomic selection, especially when the rate of missing genotypes is high and the reference population is small. | Ziqing Weng Zhe Zhang Xiangdong Ding Weixuan Fu Peipei Ma Chonglong Wang Qin Zhang | 2012 | Journal of Animal Science and Biotechnology2012，3，1： | 2 |
| 15 | Proteomic profiling of early degenerative retina of RCS rats显示文摘AIM: To identify the underlying cellular and molecular changes in retinitis pigmentosa(RP).METHODS: Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole-orbitrap MS/MS, to identify the significant differentially expressed proteins(DEPs). Bioinformatics analyses, including Gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway annotation and upstream regulatory analysis, were then performed on these retina proteins. Finally, a Western blotting experiment was carried out to verify the difference in the abundance of transcript factor E2F1.RESULTS: In this study, we identified a total of 2375 protein groups from the retinal protein samples of RCS rats and non-dystrophic congenic controls. Four hundred thirty-four significantly DEPs were selected by Student's t-test. Based on the results of the bioinformatics analysis, we identified mitochondrial dysfunction and transcription factor E2F1 as the key initiation factors in early retinal degenerative process.CONCLUSION: We showed that the mitochondrial dysfunction and the transcription factor E2F1 substantially contribute to the disease etiology of RP. The results provide a new potential therapeutic approach for this retinal degenerative disease. | Zhi-Hong Zhu Yan Fu Chuan-Huang Weng Cong-Jian Zhao Zheng-Qin Yin | 2017 | International Journal of Ophthalmology(English edition)2017，10，6： | 2 |
| 16 | Flotation performance of a novel Gemini collector for kaolinite at low temperature显示文摘How to sustainably produce bauxite by effective reverse froth flotation of kaolinite at low temperature is an urgent problem to be solved in the field of mineral processing.In this work,a novel amino-based Gemini surfactant butadiyl-1,4-bis(dimethyl dodecylammonium bromide)(BBDB) was prepared and first utilized as a novel collector for kaolinite flotation.Its flotation performance for kaolinite was compared with that of the common monomolecular surfactant 1-dodecylamine(DDA) by micro-flotation tests.The tests results indicated that 95% kaolinite recovery was obtained using 2.0×10^(-4) mol/L BBDB at 25℃ which was half of the dosage when DDA obtained the maximum kaolinite recovery of81%.At extremely low temperature(0℃),3.0×10^(-4) mol/L BBDB could still collect 91% kaolinite,while DDA showed a frustrating ability.The contact angle tests indicated that BBDB could still significantly improve the hydrophobicity of the kaolinite surface(contact angle 71.7°) than DDA(contact angle only25.8°) at 0℃.The Krafft point comparison tests indicated that BBDB had a much lower Krafft point(below0℃) than DDA.Fourier transform infrared spectroscopy(FTIR)-spectrum analysis and zeta potential measurements showed that BBDB was physically adsorbed on the surface of kaolinite through electrostatic interaction. | Shiyong Zhang Zhiqiang Huang Hongling Wang Rukuan Liu Chen Cheng Shuyi Shuai Yajing Hu Zhiqun Guo Xinyang Yu Guichun He Weng Fu | 2021 | International Journal of Mining Science and Technology2021，31，6： | 2 |
| 17 | Experimental quantum secure network with digital signatures and encryption显示文摘Cryptography promises four information security objectives,namely,confidentiality,integrity,authenticity and non-repudiation,to support trillions of transactions annually in the digital economy.Efficient digital signatures,ensuring integrity,authenticity and non-repudiation of data with information-theoretical security are highly urgent and intractable open problems in cryptography.Here,we propose a high-efficiency quantum digital signature(QDS)protocol using asymmetric quantum keys acquired via secret sharing,one-time universal_(2)hashing and a one-time pad.We just need to use a 384-bit key to sign documents of lengths up to 2~(64)with a security bound of 10^(-19).If a one-megabit document is signed,the signature efficiency is improved by more than 10~8 times compared with previous QDS protocols.Furthermore,we build the first all-in-one quantum secure network integrating information-theoretically secure communication,digital signatures,secret sharing and conference key agreement and experimentally demonstrate this signature efficiency advantage.Our work completes the cryptography toolbox of the four information security objectives. | Hua-Lei Yin Yao Fu Chen-Long Li Chen-Xun Weng Bing-Hong Li Jie Gu Yu-Shuo Lu Shan Huang Zeng-Bing Chen | 2023 | National Science Review2023，10，4： | 2 |
| 18 | Effect of Boron on Delayed Fracture Resistance of Medium-Carbon High Strength Spring Steel显示文摘The delayed fracture behavior of medium-carbon high strength spring steel containing different amounts of boron (0.000 5%, 0.001 6%) was studied using sustained load delayed fracture test. The results show that delayed fracture resistance of boron containing steels is higher than that of conventional steel 60Si2MnA at the same strength level and it increases with the increase of boron content from 0.000 5% to 0.001 6%. The delayed fracture mode is mainly intergranular in the boron containing steels tempered at 350 ℃, which indicates that the addition of boron does not change the fracture character. However, the increase of boron content enlarges the size of the crack initiation area. Further study of phase analysis indicates that most boron is in solid solution, and only a very small quantity of boron is in the M3(C, B) phase. | NIE Yi-hong HUI Wei-jun FU Wan-tang WENG Yu-qing | 2007 | Journal of Iron and Steel Research(International)2007，14，6： | 2 |
| 19 | Temperature-and thickness-dependence of robust out-of-plane ferroelectricity in CVD grown ultrathin van der Waals a-In2Se3 layers显示文摘Two-dimensional(2D)ferroelectric materials with unique structure and extraordinary optoelectrical properties have attracted intensive research in the field of nanoelectronic and optoelectronic devices,such as optical sensors,transistors,photovoltaics and non-volatile memory devices.However,the transition temperature of the reported ferroelectrics in 2D limit is generally low or slightly above room temperature,hampering their applications in high-temperature electronic devices.Here,we report the robust high-temperature ferroelectricity in 2D a-In2Se3,grown by chemical vapor depostion(CVD),exhibiting an out-of-plane spontaneous polarization reaching above 200℃.The polarization switching and ferroelectric domains are observed in In2Se;nanoflakes in a wide temperature range.The coercive field of the CVD grown ferroelectric layers ilustrates a room-temperature thickness dependency and increases drastically when the film thickness decreases;whereas there is no large variance in the coercive field at dfterent termperature from the samples with identical thickness.The results show the stable ferroelectricit of In2Se3 nanoflakes maintained at high temperature and open up the opportunities of 2D materials for novel applications in high-temperature nanoelectronic devices. | Weng Fu Io Shuoguo Yuan Sin Yi Pang Lok Wing Wong Jiong Zhao Jianhua Hao | 2020 | Nano Research2020，13，7： | 2 |
| 20 | Solvent extraction of zinc from ammoniacal/ammonium chloride solutions by a sterically hindered β-diketone and its mixture with tri-n-octylphosphine oxide显示文摘 | FU Weng CHEN Qi-yuan WU Qian | 2010 | Hydrometallurgy2010，100，34： | 1 |
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