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| 1 | Serial imaging of human embryonic stem-cell engraftment and teratoma formation in live mouse models显示文摘表示为基于 radiopharmaceutical 的成像为生物体之发光成像或 HSV1 thymidine kinase (HSV1-TK ) 编码任何一个萤火虫酶(fLuc ) 的记者 transgene 的 lentiviral 向量的二种新类型被构造监视人的胚胎的干细胞(hESC ) 在在移植以后的活老鼠的嫁接和增长。任何一个 transgene 的组成的表示没在文化改变 hESCs 的性质。我们下次在 SCID 鼠标监视了 teratomas 的形成到测试(1 ) 是否修改基因的 hESCs 维持他们的发展 pluripotency,并且(2 ) 是否支撑了记者基因表示,允许 noninvasive,在一个活鼠标模型的 hESC 衍生物的整个身体的成像。我们在接种以后从修改基因的房间以及野类型的 hESCs 2-4 月的两种类型观察了 teratoma 形成。用一个光成像系统,从 fLuc-transduced hESCs 的生物体之发光容易在在摸得出的肿瘤能被检测以前,长忍受 teratomas 的老鼠被检测。开发一个 noninvasive 成像方法对诊所更容易地可译,我们也利用了 HSV1-TK 和它的特定的底层, 1-(2 鈥 ? deoxy-2 鈥 ?fluoro- 尾 - D-arabinofuranosyl )-5-[125I]iodouracil ([125I ] FIAU ) ,记者 / 探查对。在全身的管理以后,[125I ] FIAU 是仅仅由编码 transgene 的 HSV1-TK 酶的 phosphorylated 并且在 transduced 以内保留(并且移植) 房间,由单个光子的排放允许敏感、量的成像计算了断层摄影术。象这些那样的 Noninvasive 成像方法可以使我们能在实时接受者以内重复地监视移植人的干细胞的存在和分发在上一通过记者基因的表达式长期。 | Martin G Pomper Holly Hammond Xiaobing Yu Zhaohui Ye Catherine A Foss Doris D Lin James J Fox Linzhao Cheng | 2009 | Cell Research2009，19，3： | 9 |
| 2 | 肝脏移植治疗不可切除结直肠癌肝转移的前瞻性试验性研究显示文摘背景和目的无法手术切除的结直肠癌肝转移(Colorectal Liver Metastases,CLMs)患者预后差,存活时间极少超过5年。尽管对部分肝脏原发及继发恶性肿瘤患者施行肝脏移植可获得较佳的预后,但目前仍普遍认为,结直肠癌肝转移是肝移植的绝对禁忌证。1995年之前,曾针对结直肠癌肝转移患者进行过数例肝脏移植,但预后不良(5年生存率仅为18%),自此放弃对此类患者进行肝脏移植。然而,目前总体肝移植生存率较当时提高30%左右。基于此,结直肠癌肝转移患者肝移植后的5年生存率有望达到50%。因此,本试验性研究旨在探讨CLM患者在接受肝移植后的潜在长期生存获益。方法自2006年11月到2011年3月,在此项前瞻性、试验性研究中,共对21名无法切除的结直肠癌肝转移患者进行肝移植术。患者入选标准包括一般状况良好(ECOG 1分或以下)、结直肠癌伴无法切除的单纯肝转移、原发肿瘤已行根治性切除以及移植前接受过至少6周的化疗。结果入组的患者肝脏肿瘤负荷及原发肠癌T/N分期相异;肝移植术前,76%(16/21)患者存在至少一线化疗进展。Kaplan-Meier推算患者1、3及5年总体生存率分别为95%、68%及60%。与预期相同,移植术后复发转移现象普遍,21例中19例在中位时间6(2~24)个月内发生局部复发或转移。其中,单个肺转移灶7例;其余多部位(包括肺)转移者9例,包括移植肝新发转移7例,骨转移5例,卵巢或肾上腺转移3例,腹膜转移1例,腹主动脉旁淋巴结转移2例。2例发生直肠癌局部复发。然而,绝大多数的转移癌可经手术切除或射频治疗,且经过干预后,中位随访时间27个月(8~60个月),33%(7例)患者无病生存。肝移植术前肝脏肿瘤负荷、初次手术距肝移植的时间(肠癌切除距肝移植超过2年者生存获益更显著)以及化疗后肿瘤进展均为影响预后的显著性因素。结论研究结果表明,对于此类患者人群,肝移植术后总体生存率显著高于化疗,而化疗为该患者人群现有的唯一治疗方式。此外,本研究显示的总体生存率与可切除的结直肠癌肝转移患者以及接受再次肝移植的非恶性肿瘤患者情况相当。同时,对预后因素进行手术策略筛选,应可进一步改善患者预后。(临床研究注册编号ClinicalT rials.gov:NCT01311453)。 | Hagness M Foss A Line PD 张信华 | 2013 | 消化肿瘤杂志(电子版)2013，5，4： | 8 |
| 3 | Plecanatide and dolcanatide, novel guanylate cyclase-C agonists, ameliorate gastrointestinal inflammation in experimental models of murine colitis显示文摘AIM: To evaluate the effect of orally administeredplecanatide or dolcanatide, analogs of uroguanylin, on amelioration of colitis in murine models.METHODS: The cyclic guanosine monophosphate(cG MP) stimulatory potency of plecanatide and dolcanatide was measured using a human colon carcinoma T84 cellbased assay. For animal studies all test agents were formulated in phosphate buffered saline. Sulfasalazine or 5-amino salicylic acid(5-ASA) served as positive controls. Effect of oral treatment with test agents on amelioration of acute colitis induced either by dextran sulfate sodium(DSS) in drinking water or by rectal instillation of trinitrobenzene sulfonic(TNBS) acid, was examined in BALB/c and/or BDF1 mice. Additionally, the effect of orally administered plecanatide on the spontaneous colitis in T-cell receptor alpha knockout(TCRα-/-) mice was also examined. Amelioration of colitis was assessed by monitoring severity of colitis, disease activity index and by histopathology. Frozen colon tissues were used to measure myeloperoxidase activity.RESULTS: Plecanatide and dolcanatide are structurally related analogs of uroguanylin, which is an endogenous ligand of guanylate cyclase-C(GC-C). As expected from the agonists of GC-C, both plecanatide and dolcanatide exhibited potent cG MP-stimulatory activity in T84 cells. Once-daily treatment by oral gavage with either of these analogs(0.05-0.5 mg/kg) ameliorated colitis in both DSS and TNBS-induced models of acute colitis, as assessed by body weight, reduction in colitis severity(P < 0.05) and disease activity index(P < 0.05). Amelioration of colitis by either of the drug candidates was comparable to that achieved by orally administered sulfasalazine or 5-ASA. Plecanatide also effectively ameliorated colitis in TCRα-/- mice, a model of spontaneous colitis. As dolcanatide exhibited higher resistance to proteolysis in simulated gastric and intestinal juices, it was selected for further studies. CONCLUSION: This is the first-ever study reporting the therapeutic utility of GC-C agonists as a new class of orally delivered and mucosally active drug candidates for the treatment of inflammatory bowel diseases. | Kunwar Shailubhai Vaseem Palejwala Krishna Priya Arjunan Sayali Saykhedkar Bradley Nefsky John A Foss Stephen Comiskey Gary S Jacob Scott E Plevy | 2015 | World Journal of Gastrointestinal Pharmacology and Therapeutics2015，6，4： | 5 |
| 4 | Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models显示文摘AIM To investigate the effects of plecanatide and dolcanatide on maintenance of paracellular permeability, integrity of tight junctions and on suppression of visceral hypersensitivity. METHODS Transport of fluorescein isothiocyanate(FITC)-dextran was measured to assess permeability across cell monolayers and rat colon tissues. Effects of plecanatide and dolcanatide on the integrity of tight junctions in Caco-2 and T84 monolayers and on the expression and localization of occludin and zonula occludens-1(ZO-1) were examined by immunofluorescence microscopy. Anti-nociceptive activity of these agonists was evaluated in trinitrobenzene sulfonic acid(TNBS)-induced inflammatory as well as in non-inflammatory partial restraint stress(PRS) rat models. Statistical significance between the treatment groups in the permeability studies were evaluated using unpaired t-tests.RESULTS Treatment of T84 and Caco-2 monolayers with lipopolysaccharide(LPS) rapidly increased permeability, which was effectively suppressed when monolayers were also treated with plecanatide or dolcanatide. Similarly, when T84 and Caco-2 monolayers were treated with LPS, cell surface localization of tight junction proteins occludin and ZO-1 was severely disrupted. When cell monolayers were treated with LPS in the presence of plecanatide or dolcanatide, occludin and ZO-1 were localized at the cell surface of adjoining cells, similar to that observed for vehicle treated cells. Treatment of cell monolayers with plecanatide or dolcanatide without LPS did not alter permeability, integrity of tight junctions and cell surface localization of either of the tight junction proteins. In rat visceral hypersensitivity models, both agonists suppressed the TNBS-induced increase in abdominal contractions in response to colorectal distension without affecting the colonic wall elasticity, and both agonists also reduced colonic hypersensitivity in the PRS model. CONCLUSION Our results suggest that activation of GC-C signaling might be involved in maintenance of barrier function, possibly through regulating normal localization of tight junction proteins. Consistent with these findings, plecanatide and dolcanatide showed potent antinociceptive activity in rat visceral hypersensitivity models. These results imply that activation of GC-C signaling may be an attractive therapeutic approach to treat functional constipation disorders and inflammatory gastrointestinal conditions. | Illona-Marie Boulete Anusha Thadi Catherine Beaufrand Viren Patwa Apoorva Joshi John A Foss E Priya Eddy Helene Eutamene Vaseem A Palejwala Vassilia Theodorou Kunwar Shailubhai | 2018 | World Journal of Gastroenterology2018，24，17： | 5 |
| 5 | Optimising islet engraftment is critical for successful clinical islet transplantation显示文摘 | O Korsgren T Lundgren M Felldin A Foss B Isaksson J Permert N H Persson E Rafael M Rydén K Salmela A Tibell G Tufveson B Nilsson | 2008 | Diabetologia2008，，2： | 2 |
| 6 | Growth and oxygen consumption in normal and 02 supersaturated water, and interactive effects of 02 saturation and ammonia on growth in spotted wolfish (Anarhichas minor Olafsen) 显示文摘 | FOSS A VOLLEN T QIESTAD V | 2003 | Aquaculture2003，224，14： | 1 |
| 7 | Compensa- tory growth in Atlantic halibut: effect of starvation and subsequent feeding on growth, maturation, feed utilization and flesh quality显示文摘 | Foss A Imsland A K Vikingstad E | 2009 | Aquaculture2009，290，34： | 1 |
| 8 | Modeling and control of a SOFC-GT based autonomous power system 显示文摘 | KANDEPU RAMBABU IMSLAND LARS FOSS BJARNE A | 2006 | Energy2006，32，4： | 1 |
| 9 | Growth and oxygen consumption in normal and O2 supersaturated water, and interactive effects of Oz saturation and ammonia on growth in spotted wolfish (Anarhichas minor Olafsen) 显示文摘 | Foss A Vollen T Oiestad V | 2003 | Aquaculture2003，224，14： | 1 |
| 10 | Dynamic line rating in the operating environment显示文摘 | Foss S D Maraio R A | | 0，，： | 1 |
| 11 | Tem- plate-synthesized nanoscopic gold particles: optical spectra and the effects of particle size and shape显示文摘 | Foss Jr C A Hornyak G L Stockert J A | 1994 | J Phys Chem1994，98，： | 1 |
| 12 | Autonomic neuropathy in nondiabetic offspring of type 2 diabetic subjects is associated with urinary albumin excretion rate and 24-h ambulatory blood pressure:The Frederica Study显示文摘 | Foss GH Vestbo E Froland A | 2001 | Diabetes2001，50，3： | 1 |
| 13 | On a Voronoi aggregative process related to a bivariate Poisson process 显示文摘 | Foss S G Zuyev S A | 1996 | Advances in Applied Probability1996，28，4： | 1 |
| 14 | Constrained quadratic stabilization of discrete time uncertain nonlinear multi-model systems using piece- wise affine state feedback 显示文摘 | SLUPPHAUG O FOSS B A | 1999 | International Journal of Control1999，72，： | 1 |
| 15 | Compensatory growth and fillet crude composition in juvenile Atlantic halibut : Effects of short term starvation periods and subsequent feeding显示文摘 | HEIDE A FOSS A STEFANSSON S O | 2006 | Aquaculture2006，261，1： | 1 |
| 16 | Eval- uation and development of a novel binocular treatment (I-BiT) system using video clips and interactive games to improve vision in children with amblyopia: study protocol for a randomised controlled trial显示文摘 | Foss A J Gregson R M MacKeith D | 2013 | Trials2013，14，1： | 1 |
| 17 | Interactive effects of oxygen saturation and ammonia on growth and blood physiology in juvenile turbot显示文摘 | FOSS A IMSLAND A K ROTH B | 2007 | Aquaculture2007，271，1234： | 1 |
| 18 | Ellipsometric study of the influence of chemical etching on thin porous sili- con structures显示文摘 | Selj J ThqSgersen A Foss S E | 2011 | Thin Solid Films2011，519，9： | 1 |
| 19 | Occurrence and distri- bution of extractable glycerol dialkyl glycerol tet- raethers in podzols 显示文摘 | Huguet A Fosse C Metzger P | 2010 | Org Geochem2010，41，3： | 1 |
| 20 | The interaction of temperature and salinity on growth and food conversion in juvenile turbot (Scophthalmus maximus)显示文摘 | IMSLAND A K FOSS A GUNNARSSON S | 2001 | Aquaculture2001，198，3： | 1 |
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