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| 1 | Glutamine prevents oxidative stress in a model of mesenteric ischemia and reperfusion显示文摘AIM: To evaluate preventative effects of glutamine in an animal model of gut ischemia/reperfusion(I/R).METHODS: Male Wistar rats were housed in a controlled environment and allowed access to food and water ad libitum. Twenty male Wistar rats were divided into four experimental groups:(1) control group(control)- rats underwent exploratory laparotomy;(2) control + glutamine group(control-GLU)- rats were subjected to laparotomy and treated intraperitoneally with glutamine 24 and 48 h prior to surgery;(3) I/R group- rats were subjected to occlusion of the superior mesenteric artery for 30 min followed by 15 min of reperfusion; and(4) ischemia/reperfusion + glutamine group(G + I/R)- rats were treated intraperitoneally with glutamine 24 and 48 h before I/R. Local and systemic injuries were determined by evaluating intestinal and lung segments for oxidative stress using lipid peroxidation and the activity of superoxide dismutase(SOD), interleukin-6(IL-6) and nuclear factor kappa beta(NFkB) after mesenteric I/R. RESULTS: Lipid peroxidation of the membrane was increased in the animals subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before the I/R procedure showed levels of lipid peroxidation similar to the control groups(P < 0.05). The activity of the antioxidant enzyme SOD was decreased in the gut of animals subjected to I/R when compared with the control group of animals not subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before I/R showed similar SOD activity to both control groups not subjected to I/R(P < 0.05). The mean area of NF-kB staining for each of the control groups was similar. The I/R group showed the largest area of staining for NF-kB. The G + I/R group had the second highest amount of staining, but the mean value was much lower than that of the I/R group(P < 0.05). For IL-6, control and control-GLU groups showed similar areas of staining. The I/R group contained the largest area of IL-6 staining, followed by the G + I/R animals; however, this area was significantly lower than that of the group that underwent I/R without glutamine(P < 0.05). CONCLUSION: These results demonstrate that pretreatment with glutamine prevents mucosal injury and improves gut and lung recovery after I/R injury in rats. | Gilmara Pandolfo Zabot Gustavo Franco Carvalhal Norma Possa Marroni Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann | 2014 | World Journal of Gastroenterology2014，20，32： | 7 |
| 2 | Glutamine prevents oxidative stress in a model of portal hypertension显示文摘AIM To evaluate the protective effects of glutamine in a model of portal hypertension(PH) induced by partial portal vein ligation(PPVL).METHODS Male Wistar rats were housed in a controlled environment and were allowed access to food and water ad libitum. Twenty-four male Wistar rats were divided into four experimental groups:(1) control group(SO)-rats underwent exploratory laparotomy;(2) control + glutamine group(SO + G)-rats were subjected to laparotomy and were treated intraperitoneally with glutamine;(3) portal hypertension group(PPVL)-rats were subjected to PPVL; and(4) PPVL + glutamine group(PPVL + G)-rats were treated intraperitoneally with glutamine for seven days. Local injuries were determined by evaluating intestinal segments for oxidative stress using lipid peroxidation and the activities of glutathione peroxidase(GPx), endothelial nitric oxide synthase(e NOS) and inducible nitric oxide synthase(i NOS) after PPVL.RESULTS Lipid peroxidation of the membrane was increased in the animals subjected to PH(P < 0.01). However, the group that received glutamine for seven days after the PPVL procedure showed levels of lipid peroxidation similar to those of the control groups(P > 0.05). The activity of the antioxidant enzyme GTx was decreased in the gut of animals subjected to PH compared with that in the control group of animals not subjected to PH(P < 0.01). However, the group that received glutamine for seven days after the PPVL showed similar GTx activity to both the control groups not subjected to PH(P > 0.05). At least 10 random, non-overlapping images of each histological slide with 200 × magnification(44 pixel = 1 μm) were captured. The sum means of all áreas, of each group were calculated. The mean areas of e NOS staining for both of the control groups were similar. The PPVL group showed the largest area of staining for e NOS. The PPVL + G group had the second highest amount of staining, but the mean value was much lower than that of the PPVL group(P < 0.01). For i NOS, the control(SO) and control + G(SO + G) groups showed similar areas of staining. The PPVL group contained the largest area of i NOS staining, followed by the PPVL + G group; however, this area was significantly smaller than that of the group that underwent PH without glutamine(P < 0.01).CONCLUSION Treatment with glutamine prevents gut mucosal injury after PH in rats. | Gilmara Pandolfo Zabot Gustavo Franco Carvalhal Norma Possa Marroni Francielli Licks Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann | 2017 | World Journal of Gastroenterology2017，23，25： | 3 |
| 3 | Global Pollution Monitoring of PCBs and Organochlorine Pesticides Using Skipjack Tuna as a Bioindicator显示文摘 | D. Ueno S. Takahashi H. Tanaka A. N. Subramanian G. Fillmann H. Nakata P. K. S. Lam J. Zheng M. Muchtar M. Prudente K. H. Chung S. Tanabe | 2003 | Archives of Environmental Contamination and Toxicology2003，，3： | 2 |
| 4 | U- rinary PAH metabolites as biomarkers of exposure in a- quatic environments 显示文摘 | FILLMANN G WATSON G M HOWSAM M | 2004 | Environmental Science & Tech- nology2004，38，： | 1 |
| 5 | Petroleum and PAH contamination of the Black Sea 显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollution Bulletin2002，44，： | 1 |
| 6 | The use of steroid makers to assess sewage contamination of the Black Sea显示文摘 | READ MANJ W FILLMANN G TOLOSA I | 2005 | Marine Pollution Bulletin2005，50，： | 1 |
| 7 | Petroleum and PAH contamination of the Black Sea显示文摘 | Readman J W Fillmann G Tolosa I | | 0，，01： | 1 |
| 8 | Petroleum and PAH contamination of the Black Sea显示文摘 | J.W Readman G Fillmann I Tolosa J Bartocci J.-P Villeneuve C Catinni L.D Mee | 2002 | Marine Pollution Bulletin2002，，1： | 1 |
| 9 | Persistent organochlorine residues in sediments from the Black sea 显示文摘 | Readman J W Tolosa I | 2002 | Mar Pollut Bull2002，44，： | 1 |
| 10 | Petroleum and PAH contamination of the Black Sea显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollution Bulletin2002，44，1： | 1 |
| 11 | Petroleum and PAH contamination of the Black Sea 显示文摘 | Readman J W Fillmann G Talosa I | 2002 | Marine Pollution Bulletin2002，44，1： | 1 |
| 12 | Diabetes Mellitus and Anal Sphincter Pressures: An Experimental Model in Rats显示文摘 | Henrique Sarubbi Fillmann M.D. Suzana Llessuy Ph.D. Cláudio A. Marroni M.D. Ph.D. Lúcio S. Fillmann M.D. Ph.D. Norma Possa Marroni Ph.D | 2007 | Diseases of the Colon & Rectum2007，，4： | 1 |
| 13 | Petroleum and PAH contamination of the Black Sea 显示文摘 | READMAN J W FILLMANN G TOLOSA I | 2002 | Marine Pollution Bulletin2002，44，1： | 1 |
| 14 | Distribution and transportability of hexabromocyclododecane (HBCD) in the Asia-Pacific region using skipjack tuna as a bioindicator 显示文摘 | Ueno D Alaee M Marvin C Muir DCG Macinnis G Reiner E Crozier P Furdui VI Subramanian A Fillmann G | 2006 | Environ Pollut2006，144，1： | 1 |
| 15 | Effect of Boswellia serrata on Antioxidant Status in an Experimental Model of Colitis Rats Induced by Acetic Acid显示文摘 | Renata Hartmann Maria Morgan Martins Juliana Tieppo Henrique Fillmann Norma Marroni | 2012 | Digestive Diseases and Sciences2012，，8： | 1 |
| 16 | Petroleum and PAH contamination of the Black Sea显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollution Billetin2002，44，： | 1 |
| 17 | Persistent organochlorine residues in sediments from the Black Sea 显示文摘 | Fillmann G Readman J W Tolosa I | 2002 | Mar Pollut Bull2002，44，： | 1 |
| 18 | Petroleum and PAH contamination of the Black Sea显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollution Bulletin2002，44，1： | 1 |
| 19 | Petroleum and PAH contamination of the Black Sea显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollut Bull2002，44，1： | 1 |
| 20 | Petroleum and PAH contamination of the Black Sea 显示文摘 | Readman J W Fillmann G Tolosa I | 2002 | Marine Pollution Bulletin2002，44，1： | 1 |
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