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| 1 | Nicotine-induced upregulation of antioxidant protein Prx 1 in oral squamous cell carcinoma显示文摘Nicotine is a source of exogenous oxidative stress, which is associated with the pathogenesis of numerous diseases including oral squamous cell carcinoma (OSCC), whereas an antioxidant protein, peroxiredoxin 1 (Prx 1), plays an important role in the modulation of this condition. This study was to investigate the association between Prx 1 and tobacco-induced oxidative stress. The expression of Prx 1 and GST in OSCC Tca8113 cells, which were pre-treated with nicotine, was determined. In the present study, MTT assay, reactive oxygen species (ROS) assay, RT-PCR and Western blot analyses, respectively, were conducted to assess cell viability, ROS level, and expression level of Prx 1 and GST in nicotine-treated Tca8113 cells. Nuclear factor kappa B (NF- B) expression was detected by immuno-fluorescence. Our results showed the growth of Tca8113 cells was increased in a dose-dependent manner when cells were treated with nicotine at concentrations from 0.1 to 10 mol/L, but the proliferation of the cells decreased at 100 mol/L. ROS levels increased in all groups treated with nicotine at concentrations of 0.1, 1, 10, or 100 mol/L for 24h. Prx 1 and GST mRNA and protein expression were up-regulated in cells treated with nicotine for the same time at different concentrations or at the same concentration for different times (P<0.05). NF-B was translocated from cytoplasm to nucleus, the expression of NF- B was increased in nucleus. These results suggest that up-regulation of Prx1 expression appears to be associated with tobacco-induced oxidative stress, which may play an important role in the pathogenesis of OSCC. | ZHAO YanHua ZHANG Min YAN Fei CASTO Bruce C TANG XiaoFei | 2013 | Chinese Science Bulletin2013，58，16： | 7 |
| 2 | 笋芽顶端分生组织在竹秆横切面形态形成中的作用与机制研究显示文摘竹秆的横切面主要由表皮、皮层、维管组织、基本组织及髓腔(早期为髓组织)组成。这些组织均由笋芽茎顶端分生组织分化发育而来,且其在生长发育过程中促使了竹秆横切面形态(本文特指:竹秆横切面外轮廓形状、围度及壁-腔结构)的形成。然而,人们对于这一生长发育过程的生物学机制知之甚少。本研究利用解剖学、数学模拟等方法,对 20 种具不同秆径竹种的笋芽顶端分生组织进行了分析。研究结果发现,笋芽顶端分生组织指状形态的维持是竹子产生圆柱形竹秆的基础;在顶端分生组织细胞数目增加的同时,线性增加原体细胞数目应是竹子在演化过程中产生大径竹秆且保持规则壁-腔结构的方式。进一步通过研究一个竹壁增厚且壁-腔架构紊乱的稳定变异体———实肚竹( Phyllostachys nidularia f. farcta)发现,其笋芽顶端分生组织细胞数目减少且原体/原套细胞数比值降低使其产生了高比例的壁组分组织(指基本分生组织与肋状分生组织)与低比例的髓组织。上述 2 种组织的比例失衡,使得实肚竹笋芽在后续发育中壁组分组织随机挤入到了髓组织中,最终形成了实肚竹异常的竹秆横切面形态(竹壁多样化增厚且壁-腔结构紊乱)。此外,相对较小的顶端分生组织,使得实肚竹笋芽产生了较少的内源激素,如其生长素含量仅约为其原种篌竹的 67. 5%,各细胞分裂素含量也显著低于篌竹笋芽。转录组分析进一步发现,在实肚竹笋芽中与激素及代谢相关的功能基因呈显著下调表达。这些生理与分子变化最终导致了实肚竹竹秆矮化、秆径减小且生物量降低。本论文的研究结果为研究竹秆横切面发育提供了一个重要的视角,同时也为实肚竹厚壁茎秆的形成提出了一个合理的机制。 | Wang Y Sun X Ding Y Fei Z Jiao C Fan M Yao B Xin P Chu J Wei Q 王雨珺 魏强 | 2019 | 世界竹藤通讯2019，17，3： | 5 |
| 3 | Haploid Induction via In vitro Gynogenesis in Tomato(Solanum lycopersicum L.)显示文摘In order to determine the potential for haploid induction via in vitro gynogenesis in tomato, the ovules and protoplasts of embryo sacs from the hybrids Zhongza 101 and Zhongza 105 were cultured. An efficient method of ovule isolation was established in this study. Using this method, 100-150 ovules could be isolated from one ovary. Isolated ovules were cultured on three induction media to induce gynogenesis in vitro. During culture, ovules were enlarged markedly, with opaque white color. When observed microscopically, there were cell divisions and cell clumps in embryo sacs. Subsequently, the cell clumps in embryo sacs ceased growth, likely because the integument grew faster than embryo sacs did and hindered the further development of embryo sacs. Therefore, subsequent callus morphogenesis might be originated from the integument. Thousands of calli from the two tomato varieties were obtained. Five diploid plants were regenerated after 15 months of subculturing. To eliminate the hindering effect of integument on embryo sac cells, the protoplasts of embryo sacs were prepared and cultured. After 48 hours of culture, the protoplasts of embryo sacs doubled in size and gradually formed clusters of cells. These results suggested that gynogenesis might be a potential way for haploid induction in tomato. | ZHAO He WANG Xiao-xuan DU Yong-chen ZHU De-wei GUO Yan-mei GAO Jian-chang LI Fei John C Snyder | 2014 | Journal of Integrative Agriculture2014，13，10： | 3 |
| 4 | A fundamental theorem for the aggregation problem of Input-output analysis显示文摘 | | 1955 | Econometrica1955，24，： | 1 |
| 5 | MR Imaging of non-alcoholic Wernicke encephalopathy:a follow-up study显示文摘 | 2hong C lin L Fei G | 2005 | AJNR Am J Neuroradiol2005，26，9： | 1 |
| 6 | High-Pressure Iron-Sulfur Compound, Fe3S2, and Melting Relations in the Fe-FeS System 显示文摘 | FEI Y BERTKA C M FINGER L W | 1997 | Science1997，275，3： | 1 |
| 7 | Effects of moistructure of glycerol plasticized soy protein显示文摘 | Pu C Li N Z Fei F C | 2005 | Macromol Biosci2005，5，： | 1 |
| 8 | Large-aperture YCOB Crystal Growth for Frequency Conversion in the High Average Power Laser System显示文摘 | Yifing Fei Chaia B H T Ebbers C A | 2006 | Journal of Crystal Growth2006，290，： | 1 |
| 9 | Fetal growth indicators and perfluorinated chemicals: a study in the Danish National Birth Cohort 显示文摘 | Fei C McLaughlin J K Tarone R E | 2008 | American journal of epidemiology2008，168，1： | 1 |
| 10 | Structure,function and immunolocalization of a proton coupled amino acid trans porter (hPAT1) in the human intestinal cell line Caco-2显示文摘 | Chen Z Fei Y J Anderson C M H | 2003 | The Journal of Physiology2003，546，2： | 1 |
| 11 | Statistical debugging: A hypothe- sis testing-based approach 显示文摘 | LIU C FEI L YAN X | 2006 | IEEE Transactions on Software Engineering2006，32，10： | 1 |
| 12 | Effects of soybean oligosac-charides on antioxidant enzyme activities and insulin resis-tance in pregnant women with gestational diabetes melli-tus 显示文摘 | Fei BB Ling L Hua C | 2014 | Food Chem2014，158，5： | 1 |
| 13 | Chemotherapy with paclitaxel,ifosfamide,and cisplatin for the treatment of squamous cell cervical cancer:the experience of Monza显示文摘 | G Zanetta F Fei C Mangioni | 2000 | Semin Oncol2000，27，1： | 1 |
| 14 | Beneficial properties for insulin absorption using superporous hydrogel containing interpenetrating polymer network as oral delivery vehicles 显示文摘 | Yin L C Ding J Y Fei L K | 2008 | International Journal of Pharmaceutics2008，350，12： | 1 |
| 15 | Relationships betweenknowledge inertia, organizational learning and organi- zation innovation 显示文摘 | Liao S Fei W C Liu C T | 2008 | Technovation2008，28，4： | 1 |
| 16 | Effects of ultrasonic on prepa- ration of Alumina powder by wet chemical method 显示文摘 | Chert C Wang A Fei L | 2011 | Advanced Science Letters2011，4，3： | 1 |
| 17 | Isolation of a strain of Acidithiobacillus caldus and its role in bioleaching of chalcopyrite显示文摘 | ZHOU Q G BO F BO Z H XI L JIAN G FEI L F HUA C X | 2007 | World Journal of Microbiology and Biotechnology2007，23，9： | 1 |
| 18 | Diagnosis of periprosthetic infection:novel developments显示文摘 | Chen A Fei J Deirmegian C | 2014 | J Knee Surg2014，27，4： | 1 |
| 19 | MCP-1 induces inflammatoryactivation of human tubular epithelial cells:involvement of the transcription factors,nuclear factor-κB and activating protein-1显示文摘 | VIEDT C DECHEND R FEI J | 2002 | Am Soc Nephrol2002，13，6： | 1 |
| 20 | Apoptosis occurs in implantation site of the rhesus monkey during early stage of pregnaney显示文摘 | Fei G Peng W Xin-Lei C | 2001 | Contraception2001，64，3： | 1 |
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