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| 1 | Brainstem gliomas--retrospective analysis of 86 patients显示文摘 | Ueoka DI Nogueira J Campos JC Maranhao Filho P Ferman S Lima MA | 2009 | 中国神经肿瘤杂志2009，7，3： | 3 |
| 2 | Simplified three-dimensional culture system for long-term expansion of embryonic stem cells显示文摘AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells(ESCs) labeled with enhanced yellow fluorescent protein were cultured in three-dimensional(3-D) self-assembling scaffolds and compared with traditional two-dimentional(2-D) culture techniques requiring mouse embryonic fibroblast feeder layers or leukemia inhibitory factor. 3-D scaffolds encapsulating ESCs were prepared by mixing ESCs with polyethylene glycol tetra-acrylate(PEG-4-Acr) and thiolfunctionalized dextran(Dex-SH). Distribution of ESCs in 3-D was monitored by confocal microscopy. Viability and proliferation of encapsulated cells during long-term culture were determined by propidium iodide as well as direct cell counts and PrestoB lue(PB) assays. Genetic expression of pluripotency markers(Oct4, Nanog, Klf4, and Sox2) in ESCs grown under 2-D and 3-D cultureconditions was examined by quantitative real-time polymerase chain reaction. Protein expression of selected stemness markers was determined by two different methods, immunofluorescence staining(Oct4 and Nanog) and western blot analysis(Oct4, Nanog, and Klf4). Pluripotency of 3-D scaffold grown ESCs was analyzed by in vivo teratoma assay and in vitro differentiation via embryoid bodies into cells of all three germ layers. RESULTS: Self-assembling scaffolds encapsulating ESCs for 3-D culture without the loss of cell viability were prepared by mixing PEG-4-Acr and Dex-SH(1:1 v/v) to a final concentration of 5%(w/v). Scaffold integrity was dependent on the degree of thiol substitution of Dex-SH and cell concentration. Scaffolds prepared using Dex-SH with 7.5% and 33% thiol substitution and incubated in culture medium maintained their integrity for 11 and 13 d without cells and 22 ± 5 d and 37 ± 5 d with cells, respectively. ESCs formed compact colonies, which progressively increased in size over time due to cell proliferation as determined by confocal microscopy and PB staining. 3-D scaffold cultured ESCs expressed significantly higher levels(P < 0.01) of Oct4, Nanog, and Kl4, showing a 2.8, 3.0 and 1.8 fold increase, respectively, in comparison to 2-D grown cells. A similar increase in the protein expression levels of Oct4, Nanog, and Klf4 was observed in 3-D grown ESCs. However, when 3-D cultured ESCs were subsequently passaged in 2-D culture conditions, the level of these pluripotent markers was reduced to normal levels. 3-D grown ESCs produced teratomas and yielded cells of all three germ layers, expressing brachyury(mesoderm), NCAM(ectoderm), and GATA4(endoderm) markers. Furthermore, these cells differentiated into osteogenic, chondrogenic, myogenic, and neural lineages expressing Col1, Col2, Myog, and Nestin, respectively. CONCLUSION: This novel 3-D culture system demonstrated long-term maintenance of mouse ESCs without the routine passaging and manipulation necessary for traditional 2-D cell propagation. | Christina McKee Mick Perez-Cruet Ferman Chavez G Rasul Chaudhry | 2015 | World Journal of Stem Cells2015，7，7： | 2 |
| 3 | Use of intra-arterial chemotherapy for retinoblastoma:results of a survey显示文摘·AIM: To obtain baseline knowledge about the current use of intra-arterial chemotherapy(SSOAIC) in centers worldwide.·METHODS: A survey including questions about the use of SSOAIC was emailed to retinoblastoma experts.·RESULTS:Seventy-nine(response rate 69.9%) doctors from 63 centers in 35 countries responded. Thirty-one centers from 19 countries use SSOAIC. Twelve performed more than 50 procedures. Melphalan is the most commonly used drug but 15 centers use more than one drug. First line therapy for advanced unilateral disease is the most common use of SSOAIC(74.2%). Centers with larger experience(>50 applications) were less likely using melphalan alone(P =0.06) and significantly more likely using SSOAIC in more situations such as second line in preference to radiotherapy P =0.05. Nineteen(61.2%)stated that SSOAIC improved their results and 21(77.8%)reported less toxicity compared to other treatments.Three centers reported that SSOAIC did not improve their results. There were regional variations in the use of SSOAIC which is used more frequently as secondary treatment in Europe compared to the USA and Japan.Ten centers identified cost is the major limiting factor for SSOAIC.· CONCLUSION: SSOAIC is used in an increasing number of centers worldwide with regional variations.Centers with more experience in SSOAIC use it in more situations including other drugs than melphalan. The majority of the centers using this technique reportedimproved results and few complications. | Nathalia Grigorovski Evandro Lucena Clarissa Mattosinho Andreu Parareda Sima Ferman Jaume Catalá Guillermo Chantada | 2014 | International Journal of Ophthalmology(English edition)2014，7，4： | 2 |
| 4 | Two-stage hierarchical video summary extraction to match low-level user browsing preferences 显示文摘 | Ferman A M Tekalp A M | 2003 | IEEE Trans on Multimedia2003，5，2： | 1 |
| 5 | Inclusion of RBD improves the diagnostic classiifcation of dementia with Lewy bodies显示文摘 | Ferman TJ Boeve BF Smith GE | | 0，，09： | 1 |
| 6 | Association of REM sleep behavior disorder and neurodegenerative disease may reflect an underlying synucleinopathy 显示文摘 | Boeve BF Silber MH Ferman TJ | 2001 | Mov Disord2001，16，4： | 1 |
| 7 | impaired speech perception in noise in patients with a normal audiogram显示文摘 | Ferman L Verschuure J Van Zanten B | 1993 | Audiology1993，32，： | 1 |
| 8 | Constitu- tive expression of alpha interferon by skin dendritic ceils confers resistance to infection by foot-and-mouth disease virus 显示文摘 | Bautista EM Ferman GS Gregg D | 2005 | J Virol2005，，79： | 1 |
| 9 | Interferon-a production by swine dendritic cells is inhibited during acute infection with foot-and-mouth disease virus 显示文摘 | Nfon CK Ferman GS Toka FN | 2008 | Viral Immunol2008，，21： | 1 |
| 10 | Dementia with Lewy bodies may present as dementia and REM sleep behavior disorder without parkinsonism or hallucinations显示文摘 | Ferman TJ Boeve BF Smith GE | 2002 | J Int Neuropsychol Soc2002，8，： | 1 |
| 11 | REM sleep behavior disorder and degenerative dementia: an association likely reflecting Lewy body disease显示文摘 | Boeve BF Silber MH Ferman TJ | 1998 | Neurology1998，51，： | 1 |
| 12 | Two-stage Hierarchical Video Summary Extraction to Match Low-level User Browsing Preferences显示文摘 | Ferman M Tekalp A M | 2003 | IEEE Transactions on Multimedia2003，5，2： | 1 |
| 13 | Mild cognitive impairment associated with limbic and neocortical Lewy body disease : a clini- copathological study显示文摘 | Molano J Boeve B Ferman T | 2010 | Brain2010，133，2： | 1 |
| 14 | MRS in mild cognitive impairment: early differentiation of dementia with lewy bodies and Alzheimer's disease显示文摘 | Zhang B Ferman TJ Boeve BF | 2015 | J Neuroimaging2015，25，2： | 1 |
| 15 | Mild cognitive impair- ment associated with limbic and neocortical Lewy body dis- ease: a clinicopathological study显示文摘 | Molano J Boeve B Ferman T | 2010 | Brain2010，133，2： | 1 |
| 16 | Transcatheter uterine artery embolization to treat large uterine fibroids显示文摘 | Bradley EA Reidy JF Ferman RG | 1998 | Br J Obstet Gynaecol1998，105，2： | 1 |
| 17 | Efficient giltering and clustering methods for temporal video segmentation and visual summarization 显示文摘 | FERMAN A M TEKALP A M | 1998 | Journal of Visual Communica- tion and Image Representation1998，9，4： | 1 |
| 18 | Transcatheter uterine artery embolization to treat large uterine fibroi显示文摘 | Bradley EA Reidy JF Ferman RG | 1998 | Br J Obstet Gynaecol1998，105，2： | 1 |
| 19 | Interferon-alpha production by swine dendritic cells is inhibited during acute infection with foot-and-mouth disease virus显示文摘 | Nfon C K Ferman G S Toka F N | | 0，，01： | 1 |
| 20 | Nonamnestic mild cognitive impairment progresses to dementia with Lewy bodies 显示文摘 | FERMAN TJ SMITH GE KANTARCI K | 2013 | Neurology2013，81，23： | 1 |
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