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| 1 | Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression显示文摘Recently,increasing evidence has suggested the association between gut dysbiosis and Alzheimer's disease(AD)progression,yet the role of gut microbiota in AD pathogenesis remains obscure.Herein,we provide a potential mechanistic link between gut microbiota dysbiosis and neuroinflammation in AD progression.Using AD mouse models,we discovered that,during AD progression,the alteration of gut microbiota composition leads to the peripheral accumulation of phenylalanine and isoleucine,which stimulates the differentiation and proliferation of pro-inflammatory T helper 1(Thl)cells.The brain-infiltrated peripheral Th1 immune cells are associated with the Ml microglia aaivation,contributing to AD-associated neuroinflammation.Importantly,the elevation of phenylalanine and isoleucine concentrations and the increase of Th1 cell frequency in the blood were also observed in two small independent cohorts of patients with mild cognitive impairment(MCI)due to AD.Furthermore,GV-971,a sodium oligomannate that has demonstrated solid and consistent cognition improvement in a phase 3 clinical trial in China,suppresses gut dysbiosis and the associated phenylalanine/isoleucine accumulation,harnesses neuroinflammation and reverses the cognition impairment.Together,our findings highlight the role of gut dysbiosis-promoted neuroinflammation in AD progression and suggest a novel strategy for AD therapy by remodelling the gut microbiota. | Xinyi Wang Guangqiang Sun Teng Feng Jing Zhang Xun Huang Tao Wang Zuoquan Xie Xingkun Chu Jun Yang Huan Wang Shuaishuai Chang Yanxue Gong Lingfei Ruan Guanqun Zhang Siyuan Yan Wen Lian Chen Du Dabing Yang Qingli Zhang Feifei Lin Jia Liu Haiyan Zhang Changrong Ge Shifu Xiao Jian Ding Meiyu Geng | 2019 | Cell Research2019，29，10： | 192 |
| 2 | Plant abiotic stress response and nutrient use efficiency显示文摘Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth,productivity and quality.Plants have evolved mechanisms to perceive these environmental challenges,transmit the stress signals within cells as well as between cells and tissues,and make appropriate adjustments in their growth and development in order to survive and reproduce.In recent years,significant progress has been made on many fronts of the stress signaling research,particularly in understanding the downstream signaling events that culminate at the activation of stress-and nutrient limitation-responsive genes,cellular ion homeostasis,and growth adjustment.However,the revelation of the early events of stress signaling,particularly the identification of primary stress sensors,still lags behind.In this review,we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies. | Zhizhong Gong Liming Xiong Huazhong Shi Shuhua Yang Luis R.Herrera-Estrella Guohua Xu Dai-Yin Chao Jingrui Li Peng-Yun Wang Feng Qin Jigang Li Yanglin Ding Yiting Shi Yu Wang Yongqing Yang Yan Guo Jian-Kang Zhu | 2020 | Science China(Life Sciences)2020，63，5： | 94 |
| 3 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 4 | Early neurosurgical intervention of spinal cord contusion: an analysis of 30 cases显示文摘背景有针的绳索殴打的脊柱受伤的发生在发达国家高并且现在在中国正在成长。而且,针的绳索损害主要在有长寿期待的年轻人发生。很多病人因此是为他们的生活的其余部分的轮椅界限。因此,针的绳索损害引起了世界范围的大担心。尽管有大努力,从针的绳索损害的恢复仍然保持不能令人满意。基于针的绳索殴打的病理,早 neurosurgical 干预的一个想法在这研究被提出了。有“完全”的针的绳索损害的 30 个病人全部或象美国脊柱受伤协会(亚洲) A 分类的方法 A 被学习。受伤椎骨(e) ,脊椎的列的内部固定,和为硬膜上的解压缩的双边的 laminectomy 的矫形治疗被 neurosurgical 管理直接跟随,包括恢复脑髓的液体流动和针的绳索的清创术的蛛网状的粘附的分离有伴随物 intramedullary 解压缩的坏死的织物。康复在操作以后开始了 17 天。最后的结果在 3 月康复以后被评估。皮尔森 chi 平方分析被用于统计分析。所有病人恢复了的结果走的某能力。最少的恢复病人能在稳定忍受膝关节的重量与 wheeled 重量支持和帮助走(12 个盒子, 40%) 。13 个病人(43%) 能与一双丁字拐走,一根棍子或没有任何支持。在损害以后预定操作是重要的。一扇最佳的操作时间窗户在损害以后在 4-14 天被识别。康复跟随的针的绳索殴打的结论早 neurosurgical 干预能显著地改进病人的运动。它是为针的绳索殴打的一条治疗学的途径的一个新想法并且被证明了很成功。 | ZHU Hui FENG Ya-ping Young Wise YOU Si-wei SHEN Xue-feng LIU Yan-sheng JU Gong | 2008 | Chinese Medical Journal2008，，24： | 30 |
| 5 | Ginsenoside Rgl protects against ischemic/reperfusioninduced neuronal injury through miR-144/Nrf2/ARE pathway显示文摘Ginsenoside Rg1 (Rg1),a saponin extracted from Panax ginseng,has been well documented to be effective against ischemic/ reperfusion (I/R)neuronal injury.However,the underlying mechanisms remain obscure.In the present study,we investigated the roles of Nrf2 and miR-144 in the protective effects of Rgl against I/R-induced neuronal injury.In OGD/R-treated PC12 cells,Rgl (0.01-1 μmol/L)dose-dependently attenuated the cell injury accompanied by prolonging nuclear accumulation of Nrf2,enhancing the transcriptional activity of Nrf2,as well as promoting the expression of ARE-target genes.The activation of the Nrf2/ARE pathway by Rgl was independent of disassociation with Keapl,but resulted from post-translational regulations.Knockdown of Nrf2 abolished all the protective changes of Rgl in OG-D/R-treated PC12 cells.Furthermore,Rgl treatment significantly decreased the expression of miR-144,which downregulated Nrf2 production by targeting its 3'-untranlated region after OGD/R.Knockdown of Nrf2 had no effect on the expression of miR-144,suggesting that miR-144 was an upstream regulator of Nrf2.We revealed that there was a direct binding between Nrf2 and miR-144 in PC12 cells.Application of anti-miR-144 occluded the activation of the Nrf2/ ARE pathway by Rgl in OGD/R-treated PC12 cells.In tMCAO rats,administration of Rgl (20 mg/kg).significantly alleviated ischemic injury,and activated Nff2/ARE pathway.The protective effects of Rgl were abolished by injecting of AAV-HIF-miR-144-shRNA into the predicted ischemic penumbra.In conclusion,our results demonstrate that Rgl alleviates oxidative,stress after I/R through inhibiting miR-144 activity and subsequently promoting the Nrf2/ARE pathway at the post-translational-level. | Shi-feng Chu Zhao Zhang Xin Zhou Wen-bin He Chen Chen Piao Luo Dan-dan Liu Qi-di Ai Hai-fan Gong Zhen-zhen Wang Hong-shuo Sun Zhong-ping Feng Nai-hong Chen | 2019 | Acta Pharmacologica Sinica2019，40，1： | 30 |
| 6 | Electrophysiological effects of Chinese medicine Shen song Yang xin (SSYX) on Chinese miniature swine heart and isolated guinea pig ventricular myocytes显示文摘背景沈歌杨 xin (SSYX ) 是有增加心率(HR ) 的效果的中国药的混合物。这研究试图在心和细胞的层次评估它的 electrophysiological 性质。中国缩小的猪随机被分到二个组的方法,与 SSYX 或安慰剂管理了 4 个星期(n=8 每组) 。心脏的 electrophysiological 学习(EPS ) 在药管理前后被执行。畿尼猪室的 myocytes 酶地被孤立,整个房间电压夹钳技术被用来在心脏的行动潜力(AP ) 上评估 SSYX 的效果。结果 SSYX 处理加速了 HR 从(141. | FENG Li GONG Jing JIN Zhen-yi LI Ning SUN Li-ping WU Yi-ling PU Jie-lin | 2009 | Chinese Medical Journal2009，，13： | 28 |
| 7 | Transforming growth factor-β1 in invasion and metastasis in colorectal cancer显示文摘AIM:To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and metastasis.METHODS:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent assay.TGFβ1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ hybridization.RESULTS:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<0.05.Elevated levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage Cpatients.In the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic lesion.Serum TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal cancer.It increased the invasive and metastatic potential of tumor by altering a tumor microenvironment.TGFβ1 may be used as a possible biomarke. | Bin Xiong Hong-Yin Yuan Ming-Bo Hu Feng Zhang Zheng-Zhuan Wei Ling-Ling Gong Guo-Liang Yang Department of Oncology,Affiliated Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 25 |
| 8 | Pilose antler aqueous extract promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells by stimulating the BMP-2/Smad1, 5/Runx2 signaling pathway显示文摘Peptides from Pilose antler aqueous extract(PAAE) have been shown to stimulate the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs). However, the underlying molecular mechanisms are not well understood. Here, PAAE was isolated and purified to explore the molecular mechanisms underlying PAAE’s effects on BMSCs as well as its osteoprotective effects in ovariectomized rats. Our results showed that PAAE promoted proliferation and differentiation of BMSCs to become osteoblasts by enhancing ALP activity and increasing extracellular matrix mineralization. The trabecular microarchitecture of ovariectomized rats was also found to be protected by PAAE. Quantitative reverse transcription-polymerase chain reaction(Quantitative RT-PCR) results suggest that PAAE also increased the expression of osteogenic markers including, alkaline phosphatase(ALP), runt-related transcription factor 2(Runx2), osteocalcin(OCN), bone morphogenetic protein-2(BMP-2), and collagen I(COL-I). Immunoblotting results indicated that PAAE upregulated the levels of BMP-2 and Runx2 and was associated with Smad1/5 phosphorylation. PAAE A at the concentration of 200μg·mL^-1 showed the strongest effect on proliferation and osteogenic differentiation of BMSCs after 48 h. Using matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS), we identified the molecular weight of PAAE A and found that it is less than 3000 Da and showed several significant peaks. In conclusion, PAAE activates the BMP-2/Smad1, 5/Runx2 pathway to induce osteoblastic differentiation and mineralization in BMSCs and can inhibit OVX-induced bone loss. These mechanisms are likely responsible for its therapeutic effect on postmenopausal osteoporosis. | REN Cong GONG Wei LI Feng XIE Ming | 2019 | Chinese Journal of Natural Medicines2019，17，10： | 25 |
| 9 | Expert consensus on the use of human serum albumin in critically ill patients显示文摘Introduction Human serum albumin(HSA)is a non-glycosylated,negatively charged,single-chain polypeptide composed of 585 amino acid residues with a relative molecular mass of 66.438 kD.It is synthesized by the liver at a rate of approximately 200 mg·kg^(-1)·day^(-1),with a half-life of 21 days,and subjected to catabolism in the muscles,liver,and kidneys at a rate of 4%per day.[1]Albumin,accounting for 60%of the total plasma protein,has various physiological functions,[2]such as maintaining 70%to 80%of effective plasma colloid osmotic pressure,coordinating vascular endothelial integrity,anti-oxidant and anti-inflammatory activities,maintaining the acidbase balance,and participating in the transport,distribution,and metabolism of a variety of endogenous and exogenous substances. | Yue-Tian Yu Jiao Liu Bo Hu Rui-Lan Wang Xiang-Hong Yang Xiu-Ling Shang Gang Wang Chang-Song Wang Bai-Ling Li Ye Gong Sheng Zhang Xin Li Lu Wang Min Shao Mei Meng Feng Zhu You Shang Qiang-Hong Xu Zhi-Xiong Wu De-Chang Chen | 2021 | Chinese Medical Journal2021，，14： | 22 |
| 10 | Histological Subtypes of Lung Cancer in Chinese Males from 2000 to 2012显示文摘Objective To characterize the histological and epidemiological features of male lung cancer patients in China. Methods The demographic and histological information about male lung cancer patients identified from 2000-01-01 to 2012-12-31,was collected from the Cancer Hospital of the Chinese Academy of Medical Sciences. Relative frequencies(RF) were estimated for major histological subtypes and compared according to the years of diagnosis and birth. Results The RF of adenocarcinoma(ADC) increased from 21.96% to 43.36% and the RF of squamous cell carcinoma(SCC) decreased from 39.11% to 32.23% from 2000 to 2012 in the 15 427 male lung cancer patients included in this study(Z=17.909,P<0.0001; Z=-6.117,P<0.0001). The RF of ADC increased from 28.72% in 2000-2004,36.88% in 2005-2008 to 48.61% in 2009-2012 in patients born after 1960. The age-adjusted RF of ADC in 2007-2012 increased consistently in all the investigated areas. Conclusion The increased RF of ADC in male lung cancer patients highlights the need for further investigation of the etiologic factors of these tumors. Smoke-free policies rather than modifying tobacco products should be enforced. | ZOU Xiao Nong LIN Dong Mei WAN Xia CHAO Ann FENG Qin Fu DAI Zhen YANG Gong Huan LV Ning | 2014 | Biomedical and Environmental Sciences2014，27，1： | 19 |
| 11 | Modeling rock specimens through 3D printing: Tentative experiments and prospects显示文摘Current developments in 3D printing (3DP) technology provide the opportunity to produce rock-like specimens and geotechnical models through additive manufacturing, that is, from a file viewed with a computer to a real object. This study investigated the serviceability of3 DP products as substitutes for rock specimens and rock-type materials in experimental analysis of deformation and failure in the laboratory. These experiments were performed on two types of materials as follows:(1) compressive experiments on printed sand-powder specimens in different shapes and structures, including intact cylinders, cylinders with small holes, and cuboids with pre-existing cracks, and(2) compressive and shearing experiments on printed polylactic acid cylinders and molded shearing blocks. These tentative tests for 3DP technology have exposed its advantages in producing complicated specimens with special external forms and internal structures, the mechanical similarity of its product to rock-type material in terms of deformation and failure, and its precision in mapping shapes from the original body to the trial sample(such as a natural rock joint). These experiments and analyses also successfully demonstrate the potential and prospects of 3DP technology to assist in the deformation and failure analysis of rock-type materials, as well as in the simulation of similar material modeling experiments. | Quan Jiang Xiating Feng Lvbo Song Yahua Gong Hong Zheng Jie Cui | 2016 | Acta Mechanica Sinica2016，32，1： | 19 |
| 12 | Characterization and normalization factors of abiotic resource depletion for life cycle impact assessment in China显示文摘The availability of resources for economic activities differs between regions, and the importance of the resources is consequently observed to be different within regions compared to a global scale. With the current situation in Chinese mining industry and its statistic characteristics, the characterization procedures of abiotic resource in life cycle impact assessment (LCIA) have demonstrated certain limita-tions in the Chinese materials industry. The aim of this paper is to propose new characterization and normalization factors for abiotic resource depletion categories such as metals and non-renewable en- ergy resources in a Chinese context. The actual production of abiotic resources calculated by a modi- fied model is compared to the reserve base in line with the new national standard to determine char- acterization factors in equivalence units, with antimony as the reference mineral. The normalization factors are based on the total base reserves of the most important minerals in China. A case study on primary magnesium production using the Pidgeon process is used to compare LCIA results for abiotic resource categories that are between current LCIA factors and the new Chinese factors. These factors not only reflect the importance of abiotic resource with respect to region-specific resource depletion, but also can compare with the global factors. | GAO Feng NIE ZuoRen WANG ZhiHong GONG XianZheng ZUO TieYong | 2009 | Science China(Technological Sciences)2009，52，1： | 17 |
| 13 | Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells显示文摘迟了发作的性腺机能减退(LOH ) 是仔细与在年老的男性,而是 the 的第二等的雄激素缺乏有关;机制遗体不清楚。在这研究,我们发现在年老的老鼠 Leydig 房间的那减少的睾丸激素生产与减少的 autophagic 活动被联系。主要老鼠 Leydig 房间和 TM3 老鼠 Leydig 房间线被用来在 Leydig 房间睾丸激素生产上学习 autophagic 缺乏的效果。在从小、年老的老鼠的 Leydig 房间,有 wortmannin 的处理,一个 autophagy 禁止者,禁止的 luteinising 荷尔蒙(LH ) 刺激了 steroidogenic 尖锐规章(星) 蛋白质表示和减少的睾丸激素生产。相反,有 rapamycin, autophagy 使活跃之物,在从的 Leydig 房间的提高的刺激 LH 的 steroidogenesis,然而并非幼仔的处理,老鼠。细胞内部的反应的氧种(ROS ) 层次在与 wortmannin 对待,但是仅仅在与 rapamycin 对待的年老的 Leydig 细胞减少了的年轻、年老的 Leydig 细胞被增加。而且, ROS 的增加的水平,由 H 2 O 2, 导致了刺激 LH 的 steroidogenic 抑制。最后, Beclin 1 击倒在 TM3 老鼠 Leydig 房间的减少的刺激 LH 的星表示和睾丸激素生产,它与增加的细胞内部的 ROS 被联系铺平。这些结果建议那 autophagic 缺乏与在年老的老鼠 Leydig 房间的 steroidogenic 衰落有关,它可能被细胞内部的 ROS 层次影响。 | Wei-Ren Li Liang Chen Zhi-Jie Chang Hua Xin Tao Liu Yan-Quan Zhang Guang-Yong Li Feng Zhou Yan-Qing Gong Zhe-Zhu Gao Zhong-Cheng Xin | 2011 | Asian Journal of Andrology2011，13，6： | 17 |
| 14 | Natural populations of lactic acid bacteria in douchi from Yunnan Province,China显示文摘This research was aimed at isolating and identifying the predominant lactic acid bacteria(LAB) in the traditional Chinese salt-fermented soybean food,douchi,from Yunnan,China.The predominant LAB present were isolated and identified by conventional culture-dependent methods combined with molecular biological methods.Two hundred and sixty isolates were obtained from thirty kinds of traditional fermented douchi from six cities and counties in Yunnan,and those strains were divided into twelve groups by their morphological and biochemical characteristics.Based on 16S ribosomal DNA(rDNA) sequencing and analysis,56 representative strains were identified as belonging to 6 genera and 14 species:Lactobacillus(4 spp.),Weissella(3 spp.),Pediococcus(2 spp.),Staphylococcus(2 spp.),Enterococcus(1 sp.),and Bacillus(2 spp.).The results show that douchi contains a large natural population of LAB of diverse composition from which some strains could be selected as starters for functional fermented foods.This is the first study on the original douchi from Yunnan,and the results suggest that it may be a useful source for the isolation of LAB.This study has also laid a foundation for further research on developing functional douchi products. | Chen-jian LIU Fu-ming GONG Xiao-ran LI Hai-yan LI Zhong-hua ZHANG Yue FENG Hiroko NAGANO | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，4： | 16 |
| 15 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 16 | Mutational Analysis of Region-cytotoxicity Relationship in Human Transmembrane Tumor Necrosis Factor-alpha显示文摘Objective:To determine the region of human transmembrane tumor necrosis factor-alpha (TM-TNFa), essential for cytotoxic activity a-gainst human breast cancer cell line MCF-7. Methods:Single amino-acid-substituted TM-TNFα mutant proteins (muteins) were produced by in vitro transcription linked translation techniques. The cDNA of TM-TNFα was site-directed mutagenized by recombinant PCR. Results:13 single amino-acid substituted TM-TNFα muteins were generated and assayed for cytotoxic activity. The cytotoxic activities of TM-TNFα muteins, eg, TM-TNFα-71/Lys, -28/Phe and 117/Leu were significantly decreased (P<0.01) compared to that of parent TM-TNFα, 143/Tyr decreased 4-folds, and-17/Thr,-39/Ser,ll9/His,35/Gly,95/Cys and 147/Phe decreased 1.5-2.5-folds, respectively. However, the cytotoxic activities of TM-TNFα-8/Arg, 31/Gly and 87/Phe showed no significant change. Conclusion:These results indicate that the regions associated with cytotoxic-activity of TM-TNFα are different with that of secretory TNF-lpha (S-TNFα). The inner cell region and transmembrane region of TM-TNFα are related to the cytotoxic activity of TM-TNFα. | ZHENG Fang, GONG Feili, LI Zhuoya, JIANG Xiaodan, XIONG Ping, FENG Wei, XU Yong, Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2002 | The Chinese-German Journal of Clinical Oncology2002，1，1： | 14 |
| 17 | Impact of a nationwide training program for neonatal resuscitation in China显示文摘 | XU Tao WANG Hui-shan YE Hong-mao YU Ren-jie HUANG Xing-hua WANG Dan-hua Wang Li-xin FENG Qi GONG Li-min MA Yi William Keenan Susan Niermeyer | 2012 | Chinese Medical Journal2012，，8： | 13 |
| 18 | B to O erythrocyte conversion by the recombinant α-galactosidase显示文摘背景人的组 O 红血房间在象武装冲突和自然灾祸那样的专业化输送区域有大利益。组 B 抗原不同从仅仅由一个终端的增加的组 O 抗原的在结构上连接了半乳糖残余。在我们试图把终端半乳糖从组 B 红血房间移开得到的这研究组织 O 红血 cell.Methods 牛乳糖 cDNA 被 RT-PCR 从在 China.The 的海南岛上种的 Catimor 咖啡豆子克隆为牛乳糖 cDNA 表示的向量被构造并且由 electroporation 转了进 Pichia pastoris 房间。转基因的房间被发酵克隆, recombinant 牛乳糖被离子交换层析净化。在学习 -galactosidase, 的生物化学的人物以后,我们在从组 B 变换人的红血球组织 recombinant 牛乳糖的纯净是的 O.Results 使用了它比 96% 高,它被认为对血的使用合适变换。酶地变换的人的组 O 红血房间(ECHORBC ) 展出了膜正直,新陈代谢的正直,正常房间变丑和形态学。在 ECHORBC 和任何组人的血之间没有凝结。ECHORBC 将在 monoammoniumphosphate 营养素答案在 4 点为 21 天的一个句号使结构和函数正常。有恒河猴和长臂猿的实验证明酶地变换的红血球的那输送是 safe.Conclusion ECHORBC 能被牛乳糖容易从组 B 红血房间获得消化。这研究建议 ECHORBC 能安全地并且高效地被输到病人。 | ZHANG Yang-pei GONG Feng BAO Guo-qiang GAO Hong-wei JI Shou-ping TAN Ying-xia LI Su-bo LI Li-li WANG Ying-li XU Hua XU Li-juan TIAN Shu-guang ZHANG Zhi-xin LU Qiu-shuang QIU Yan BAI Jian-shi CHEN Ji-ting | 2007 | Chinese Medical Journal2007，，13： | 13 |
| 19 | A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation显示文摘Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor. Methods Three cDNA fragments with the same 11 bp 5’ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5’ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5’ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide (RBP)] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment. pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide. Results Three cDNA fragments with the same 11 bp 5’ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5’ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual GC-resistin significantly inhibited the adipogenic differentiation. Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte. | LIU Feng GUO Xi-rong GONG Hai-xia NI Yu-hui FEI Li PAN Xiao-qin GUO Mei CHEN Rong-hua | 2006 | Chinese Medical Journal2006，，6： | 12 |
| 20 | Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice显示文摘杂种优势比也是从二父母表演的后代改善了的生物现象和优异性能生来的父母线。混合米饭是在利用杂种优势的庄稼的最成功的 apotheoses 之一。由基因表示(半自动地面防空系统)的连续分析 F1 超级混血儿的米饭 Liangyou-2186 和它的父母介绍的 Transcriptional 表明 1183 差别表示了基因( DG ),在哪个之中 DG 被发现显著地在象光合作用和碳固定那样的小径充实,并且大多数涉及碳固定小径的关键基因在 F1 混合米饭展出了起来调整的表示。而且,相应的酶的增加的分解代谢的活动和光合的效率也被检测,它联合了显示那碳固定可能在 F1 混血儿,和力量被提高在超级混血儿的米饭与收益活力和杂种优势被联系。由有收益相关的量的特点 loci (QTL ) 的相关 DG,在微分基因表示和 phenotypic 变化之间的一种潜在的关系也被发现。另外,当以前在 Arabidopsis 报导了,包含生理节奏节奏、轻的发信号的小径的一个规章的网络也被发现,它建议如此的一个网络可能也与在混合米饭的杂种优势是相关的。总的来说,现在的学习为理解在米饭位于杂种优势下面的分子的机制提供另一个看法。 | Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen Yu-Guo Xiao Lili Wang Yue-Jun Chen Bin Wu Bin Chen Yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk Thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu | 2010 | Molecular Plant2010，3，6： | 12 |
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