|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Correlation of N-myc downstream-regulated gene 1 overexpression with progressive growth of colorectal neoplasm显示文摘AIM:To study the function of N-myc downstream-regulated gene 1 (NDRG1) in colorectal carcinogenesis and its correlation with tumor lymph node metastasis,METHODS:NDRG1 was detected at its protein level by immunohistochemistry (IHC) and image analysis (IA), and NDRG1 mRNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 190 specimens including 38 normal colorectal mucosae, 31 colorectal adenomas, 45 non-metastatic colorectal carcinomas (CRCs), 38 metastatic primary CRC and subsequently regional lymph nodes respectively. At the same time, the correlations of NDRG1 with sex, age of patients and histological types of colorectal carcinomas were observed.RESULTS: NDRG1 proteins were gradually increased in colorectal carcinogenesis (P<0.05 or P<0.01). There was a significant difference in the expression of NDRG1 between non-metastatic and metastatic CRCs (P<0.05), and the correlation was positive (P<0.01, rs=0.329). However, there was no obvious difference in the expression of NDRG1 between the primary sites of CRCs and that in the metastatic sites of corresponding regional lymph nodes, nor was there an apparent difference in sex, age, and histological types.The expression of NDRG1 mRNA was generally in concordance with that of NDRG1 protein.CONCLUSION: NDRG1 gene may play an important role in colorectal carcinogenesis.In addition, NDRG1 may be a putative tumor metastasis promoter gene and is regarded as one of the molecular biological markers that can forecast early metastasis of CRCs. NDRG1 gene in the metastatic sites of regional lymph nodes may preserve its expression characteristics in the primary sites of CRCs to some extent.The expression of NDRG1 is not affected by sex, age and histological types. The role of NDRG1 in tumor metastatic process can be demonstrated by in vivo and in vitro. | ZhenWang FangWang wei-QiWang QianGao Wan-LiWei YunYang Guo-YingWang | 2004 | World Journal of Gastroenterology2004，10，4： | 33 |
| 2 | Transforming growth factor beta can be a parameter of aggressiveness of pT1 colorectal cancer显示文摘AIM: To evaluate the significance of transforming growth factor beta (TGF β) expression, in correlation with histopathological parameters, at the front of invasion in T1 colorectal cancer (CRC) and presence of metastases.METHODS: TGF β immunohistochemical expression was studied in 34 specimens of colorectal adenocarcinomas (pT1). A three-step avidin-biotinylated immuno-peroxidase (ABCu-NCL) staining technique was performed on 4-μm paraffin-embedded tissue sections with a monoclonal antibody to TGF β (Novocastra, NCL-TGFB, clone TGFB 17,dilution 1:40).RESULTS: Seventeen (50%) out of 34 lesions were positive for TGF β expression. The TGF β-positive rate in patients with vascular invasion was significantly higher than in those without vascular invasion (11/14 cases, P<0.01,P = 0.005). The TGF β-positive rate was observed in 91.7% of patients with presence of tumor budding at the front of invasion (11/12 cases, P<0.01, P = 0.0003). A statistically significant correlation was found between the presence of lymph node metastases and positive expression of TGF β (14/16 cases, P<0.01, P = 0.0001). We also observed that the TGF β-positive rates in groups with distant and non-distant metastases were 92.8% and 20% respectively,and a significant correlation between TGF β expression and distant metastasis was shown (P<0.01, P = 0.00003).CONCLUSION: The evaluation of TGF β expression of protein in association with histological parameters can be used as a parameter of the aggressiveness of pT1 CRC. | Katarzyna Guzinska-Ustymowicz Andrzej Kemona | 2005 | World Journal of Gastroenterology2005，11，8： | 25 |
| 3 | Comparative evaluation of immune response after laparoscopical and open total mesorectal excisions with anal sphincter preservation in patients with rectal cancer显示文摘AIM: The study of immune response of open versus laparoscopical total mesorectal excision with anal sphincter preservation in patients with rectal cancer has not been reported yet. The dissected retroperitoneal area that contacts directly with carbon dioxide is extensive in laparoscopic total mesorectal excision with anal sphincter preservation surgery. Tt is important to clarify whether the immune response of laparoscopic total mesorectal excision with anal sphincter preservation (LTME with ASP) in patients with rectal cancer is suppressed more severely than that of open surgery (OTME with ASP). This study was designed to compare the immune functions after laparoscopic and open total mesorectal excision with anal sphincter preservation for rectal cancer.METHODS: This study involved 45 patients undergoing laparoscopic (n=20) and open (n=25) total mesorectal excisions with anal sphincter preservation for rectal cancer.Serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor α (TNFα) were assayed preoperatively and on days 1 and 5 postoperatively. CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ natural killer cell (NK)count and immunoglobulin (IgG/IgM/IgA) were assayed preoperatively and on day 5 postoperatively. The numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells were counted using flow cytometry. An enzyme-linked immunosorbent assay (ELISA) was used for IL-2, TL-6 and TNFα determination. And IgG, IgM, and IgA were assayed using immunonephelometry.RESULTS: The demographic data of the two groups had no difference. The preoperative levels of CD3+ and CD56+ T lymphocyte count, CD3- and CD56+ NK count, serum IgG,IgM, IgA, IL-2, IL-6 and TNFα also had no significant difference in the two groups (P>0.05). The CD3+ and CD56+ T lymphocyte counts had no obvious changes after surgery in laparoscopic (d=-0.79±3.83 %) and open (d=0.42±2.09 %)groups. The CD3- and CD56+ NK counts were decreased postoperatively in both laparoscopic (d=-7.23±11.33 %) and open (d=-9.21±13.93 %) groups. The differences of the determined values of serum IgG, IgM and IgA on the fifth day after operation subtracted those before operation were -2.56±2.14 g/L, -252.35±392.94 mg/L, -506.15±912.24 mg/L in laparoscopic group, and -1.81±2.10 g/L, -282.72±356.75mg/L, -252.20±396.28 mg/L in open group, respectively. The levels of IL-2 were decreased after operation in both groups.However, the levels of IL-6 were decreased after laparoscopic surgery (d1=-23.14±263.97 ng/L and d5=-40.08±272.03 ng/L),and increased after open surgery (d1=27.38±129.14 ng/L and d5=21.67±234.31 ng/L). The TNFα levels were not elevated after surgery in both groups. There were no significant differences in the numbers of CD3+ and CD56+ T lymphocytes and CD3- and CD56+ NK cells, the levels of IgG, IgM, IgA,IL-2, IL-6 and TNFα between the two groups (P>0.05).CONCLUSION: There are no differences in immune responses between the patients having laparoscopic total mesorectal excision with anal sphincter preservation and those undergone open surgery for rectal cancer. | Jian-KunHu Zong-GuangZhou Zhi-XinChen Lan-LanWang Yong-YangYu JinLiu BoZhang LiLi YeShu Jia-PingChen | 2003 | World Journal of Gastroenterology2003，9，12： | 23 |
| 4 | Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells显示文摘AIM: To investigate the association between endogenous gene expression and growth regulation including proliferation and apoptosis induced by transforming growth factor-β1(TGF-β1) in human gastric cancer (GC) cells.METHODS: Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the main components of the TGF-β1/Smads signal pathway in human poorly differentiated GC cell line BGC-823. Localization of Smad proteins was also determined using immunofluorescence.Then, the BGC-823 cells were cultured in the presence or absence of TGF-β1 (10 ng/mL) for 24 and 48 h, and the effects of TGF-β1 on proliferation and apoptosis were measured by cell growth curve and flow cytometry (FCM)analysis. The ultrastructural features of BGC-823 cells with or without TGF-β1 treatment were observed under transmission electron microscope. The apoptotic cells were visualized by means of the terminal deoxynucleotidyl transferase (TdT)-mediated dTUPin situ nick end-labeling (TUNEL) method. Meanwhile, the expression levels of endogenous p15, p21 and Smad7 mRNA and the corresponding proteins in the cells were detected at 1, 2and 3 h after culture in the presence or absence of TGF-β1(10 ng/mL) by semi-quantitative RT-PCR and Western blot,respectively.RESULTS: The TGF-β1/Smad signaling was found to be intact and functional in BGC-823 cells. The growth curve revealed the most evident inhibition of cell proliferation by TGF-β1 at 48 h, and FCM assay showed G1 arrest accompanied with apoptosis induced by TGF-β1. The typical morphological changes of apoptosis were observed in cells exposed to TGF-β1. The apoptosis index (AI) in TGF-β1-treated cells was significantly higher than that in the untreated controls (10.7±1.3% vs 0.32±0.06%, P<0.01).The levels of p15, p21 and Smad7mRNA and corresponding proteins in cells were significantly up-regulated at 1 h, but gradually returned to basal levels at 3 h following TGF-β1(10 ng/mL) treatment.CONCLUSION: TGF-β1 affects both proliferation and apoptosis of GC cells through the regulation of p15 and p21,and induces transient expression of Smad 7 as a negative feedback modulation of TGF-β1 signal. Our results suggest a novel functional role of p21 as an accelerant of TGF-β1-mediated apoptosis in GC cells. | XueLi Yun-YanZhang Qiwang Song-BinFu | 2005 | World Journal of Gastroenterology2005，11，1： | 12 |
| 5 | In situ detection of TGF betas, TGF beta receptor II mRNA and telomerase activity in rat cholangiocarcinogenesis显示文摘AIM: Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFβs),TGFβ type Ⅱ receptor (TβRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis.METHODS: Rat liver cholangiocarcinogenesis was induced by 3'-methyl 4-dimethylazobenzene (3'Me-DAB). In situhybridization was used to examine the TGFβs) and TGFβ type Ⅱ receptor (TβRⅡ) mRNA, in situ TRAP was used to check the telomerase activity in the tissue samples.RESULTS: There was no TGFβs, TβRⅡ mRNA expression or telomerase activity in the control rat cholangiocytes. The expression of TGFβ1, TβRⅡ was increased in regenerative,hyperplastic, dysplastic cholangiocytes and cholangiocarcinoma (CC) cells. The expression of TGFβ2 mRNA was observed in only a part of hyperplastic, dysplastic cholangiocytes. TGFβ3expression was very weak, only in hyperplastic lesion. There was positive telomerase activity in the regenerative,hyperplastic, dysplastic cholangiocytes, and CC cells. Stroma fibroblasts of these lesions also showed positive TGFβs, TβRⅡ mRNA expression and telomerase activity.CONCLUSION: There were TGFβs, TβRⅡ expression and telomerase activity in hyperplastic, dysplastic cholangiocytes,cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis. Their expression or activity is important in cholangiocarcinogenesis andstroma formation. | Jian-PingLu Jian-QunMao Ming-ShengLi Shi-LunLu Xi-QiHu Shi-NengZhu ShintaroNomura | 2003 | World Journal of Gastroenterology2003，9，3： | 10 |
| 6 | 大蒜素抑制体外人肝癌细胞的侵袭能力显示文摘目的:肝癌的侵袭与转移是导致其不良预后的重要因素,目前临床缺乏针对肝癌侵袭与转移的有效治疗措施. 本研究通过人肝癌细胞培养,研究大蒜素对体外人肝癌细胞侵袭能力的影响,并在基因水平上探讨其机制. 方法:将人肝BEL-7402细胞传代,取进入指数生长期的细胞随机分为阿霉素组Human(5 mg/L)、大蒜素低、中、高剂量组(25、50、100 mg/L),另设空白对照. 处理后每30 min观察一次细胞转移相关超微结构,8 h 后用流式细胞术检测肿瘤侵袭转移抑制基因nm23-H1 和P2ras表达水平.数据用SAS 8.2软件进行X2检验. 结果:与阿霉素组相比,大蒜素处理后超微结构除凋亡表现外,大多数贴壁细胞胞质回缩,细胞之间的连接减少,空隙加大,细胞之间界限变清晰,表面的丝状微绒毛也明显减少.流式细胞检测显示,nm23-H1表达的荧光强度空白对照组为19.19,5 mg/L.阿霉素组为119.76,25 mg/L大蒜素组为84.28,50mg/L大蒜素组为92.64,100 mg/L大蒜素组为138.08,nm23-H1 表达与大蒜素呈明显剂量效应关系,大蒜素高剂量组比阿霉素组显著增强(138.08 vs 119.76,P<0.05).而P21ras 表达的荧光强度在空白对照组、阿霉素组和低、中、高剂量组分别为2.65%、3.56%、1.55%、3.22%、3.44%,都表现为阴性低表达,各组间没有明显差别. 结论:大蒜素可以抑制体外肝癌细胞的侵袭能力,其机制可能与大蒜素特异性影响BEL-7402细胞袁面的微绒毛和上调肿瘤侵袭与转移抑制基因nm23-H1的表达有关. | 钟宁 马亚兵 高海青 张志勉 程梅 由倍安 伊永亮 刘新春 | 2005 | 世界华人消化杂志2005，13，6： | 8 |
| 7 | 蟾毒灵抑制人结肠癌HCT116细胞侵袭机制探讨显示文摘目的肿瘤侵袭转移的发生是影响结肠癌治疗的重要原因,结肠癌细胞黏附能力的下降以及迁移能力的增强是促进结肠癌细胞侵袭转移的重要原因。有研究发现,蟾毒灵具有抑制结肠癌细胞侵袭能力的作用,但其作用机制尚不明确。本研究探讨蟾毒灵对人结肠癌HCT116细胞侵袭的影响,并探索其可能的作用机制。方法用蟾毒灵溶液干预结肠癌HCT116细胞,采用细胞划痕实验与细胞黏附实验检测蟾毒灵溶液对结肠癌HCT116细胞侵袭能力的影响;ELISA法检测蟾毒灵溶液干预结肠癌HCT116细胞培养基中细胞外泌TGFβ1蛋白的含量;蛋白质印迹法检测蟾毒灵干预后结肠癌HCT116细胞中P-smad3、smad4和钙黏链蛋白(E-cadherin)表达的变化;免疫荧光实验检测蟾毒灵干预后各组HCT116细胞中P-smad3与smad4蛋白表达的变化。结果细胞划痕实验结果显示,低、中、高剂量蟾毒灵均可降低HCT116细胞迁移能力;细胞黏附实验显示,对照组、低剂量组、中剂量组和高剂量组黏附细胞平均值分别为833.33±7.80、877.00±17.75、1 304.00±15.82和1 406.00±20.53,相对黏附率分别为100%、105.24%、156.48%和168.73%,差异有统计学意义,χ2=297.597,P<0.001。ELISA检测结果显示,对照组、低、中和高剂量细胞培养液中转化生长因子β(transforming growth factor-β,TGFβ1)蛋白含量分别为(1 198.78±38.96)、(1 106.58±35.76)、(1 040.80±47.47)和(987.74±37.52)pg/mL,F=16.357,P=0.005。蛋白质印迹法检测结果显示,与对照组相比,中、高剂量蟾毒灵处理后HCT116细胞中P-smad3表达呈现明显下降;而smad4蛋白表达明显上调,组间差异有统计学意义,F=56.993,P<0.001;低剂量蟾毒灵也可上调smad4蛋白的表达,t=5.342,P=0.006。各用药组HCT116细胞中E-cadherin蛋白的表达水平也随着蟾毒灵剂量的增加而增加,分别为对照组的2.514±0.385(t=4.833,P=0.008)、3.524±0.397(t=9.200,P=0.001)和3.937±0.318(t=10.675,P<0.001)倍;免疫荧光法检测发现,蟾毒灵处理后结肠癌HCT116细胞中P-smad3与smad4蛋白的表达呈现出与蛋白质印迹法检测相似的趋势。结论蟾毒灵可通过抑制TGFβ1/smad信号通路的信号传导抑制结肠癌HCT116细胞的侵袭。 | 王师英 林江 邢立凯 陈腾 | 2016 | 中华肿瘤防治杂志2016，23，8： | 5 |
| 8 | 胃癌细胞nm23H1基因表达与体内外侵袭力的关系显示文摘目的:探讨nm23H1基因与胃癌细胞体内外侵袭力的关系.方法:采用Boyden小室法测定四种胃癌细胞系MKN1、MKN45、GT3TKB、BGC823的体外侵袭力,Northern印记杂交,RT-PCR及免疫组化技术检测胃癌组织、四种胃癌细胞系的nm23H1mRNA及蛋白表达水平.结果:胃癌细胞体外侵袭力高低的顺序依次为MKN45最高(33.1±5.2,与其他三种细胞系相比P<0.01);BGC823(15.8±2.7),MKN1(14.1±4.5)次之(二者无显著差异,P>0.05),GT3TKB最低(6.3±2.5,与其他三种细胞系相比P<0.01).nm23H1基因表达与胃癌细胞体内侵袭能力呈反比关系;nm23H1基因表达与MKN45,BGC823,GT3TKB细胞体外侵袭力也呈反比关系,但与MKN1细胞体外侵袭力无明显关系.结论:nm23H1表达在评价胃癌细胞体内外侵袭力方面具有重要价值. | 孙秀菊 孙开来 付浩 王舒宝 陈峻青 | 2003 | 世界华人消化杂志2003，11，1： | 5 |
| 9 | 人源性大肠癌抗原基因的SEREX筛选显示文摘目的:寻找人源性大肠癌相关抗原基因.方法:构建3个以入Tripl Ex2噬菌体作载体的大肠癌抗原cDNA表达文库,用自体或异体大肠癌患者血清应用SEREX方法进行免疫筛选.用平板法扩增阳性克隆噬菌体,提取纯化DNA,用SfiI酶切和PCR鉴定插入片段的大小.结果:构建成3个大肠癌抗原cDNA噬菌体表达文库,滴度分别为2.39×106nfu/L,2.07×106nfu/L和1.86×106nfu/L,插入片段长度为0.5-4kb,平均分别为1.4kb,1.6kb和1.3kb.筛选发现4个阳性克隆,插入cDNA片段大小分别为2.4 kb,1.8 kb,2.3 kb和2.2 kb.结论:用SEREX方法筛选大肠癌抗原cDNA表达文库,可获得有价值的大肠癌的重组抗原基因克隆,将有利于大肠癌的早期诊断和重组疫苗的研究. | 刘宇虎 张振书 钟东 武金宝 但汉雷 赖卓胜 王亚东 张亚历 肖冰 | 2003 | 世界华人消化杂志2003，11，9： | 4 |
| 10 | Inhibitory effects of N-(4-hydrophenyl) retinamide on liver cancer and malignant melanoma cells显示文摘AIM: To investigate the effect of N-(4-hydrophenyl) retinamide (4-HPR), the derivative of retinoic acid, on inhibition of migration, invasion, cell growth, and induction of apoptosis in hepatocellular carcinoma cells (HCCs) and malignant melanoma cells.METHODS: 4-HPR was chemically synthesized. Cellular migration and invasion were assayed by Borden chamber experiment. Cell growth was assayed by MTT chromometry.Apoptosis effect was measured using Hoechst 32258 staining and flow cytometry. Gene transfection was performed with lipofectamine.RESULTS: We observed that the migration of HCC and melanoma cells was significantly suppressed by 4-HPR and the migration cells were reduced to 58±5.03 (control 201±27.2, P<0.05, n = 4) in SMMC 7721-k3 HCC, and to 254±25.04 (control 302±30.1, P<0.05, n = 4) in melanoma cells after 6-h incubation with 4-HPR. The invasion through reconstituted basement membrane was also significantly reduced by 4-HPR treatment to 11.2±3.3 in SMMC 7721-k3 HCC (control 27±13.1), and to 24.3±3.2 in melanoma cells (control 67.5±10.1, P<0.05, n = 3). Cell growth, especially in melanoma cells, was also significantly inhibited.Furthermore, 3 μmol/L of 4-HPR induced apoptosis in B16 melanoma cells (37.11±0.94%) more significantly than all-trans retinoic acid (P<0.05), but it failed to induce apoptosis in SMMC 7721-k3 HCC. The mechanism for 4-HPR-induced apoptosis was not clear, but we observed that 4-HPR could regulate p27kip1, and overexpression of cerebroside sulfotransferase (CST) diminished the apoptosis induced by 4-HPR in melanoma cells.CONCLUSION: 4-HPR is a potent inhibitor of HCC migration and inducer of melanoma cell apoptosis. CST and p27kip1 expression might be associated with 4-HPR-induced apoptosis. | Xing-Zhong Wu Li Zhang Bi-Zhi Shi Ping Hu | 2005 | World Journal of Gastroenterology2005，11，37： | 4 |
| 11 | 转化生长因子-β1在结直肠癌中的表达及与预后的关系显示文摘目的探讨转化生长因子(TGF)-β1在结直肠癌中的表达水平及与预后的关系。方法应用免疫组化技术检测52例结直肠癌病例术后标本巾TGF-β1表达水平与血管内皮生长凶子(VEGF)及临床病理特征间的相关性,Log-rank时序检验TGF61蛋白表达与生存时间的关系。结果TGF-β1表达为阴性和阳性者分别有11例和41例,而VEGF表达为阴性和阳性者分别有14例和38例,两者间有显著相关性(X^2=0.633,P〈0.01),TGF-β1表达与Dukes分期(X^2=19.866,P〈0.01)、淋巴结转移情况(X^2=13.152,P〈0.01)也显著相关。本组病例总的3年生存率为49.1%,而TGF61表达为阴性和阳性者的3年生存率分别为69.2%和20.5%(X^2=11.64,P〈0.01)。结论TGF-β1表达水平是影响结直肠癌患者预后的重要因素,临床上作为判断患者预后指标值得进一步研究。 | 范秋虹 冯一中 徐永兴 | 2006 | 中华消化杂志2006，26，8： | 3 |
| 12 | 可溶性转化生长因子βⅡ型受体对大肠癌生物学行为的影响显示文摘目的探讨可溶性转化生长因子βⅡ型受体(sTGF-βRⅡ)对大肠癌 LoVo 细胞生物学行为的影响。方法采用 DNA 重组技术构建 sTGF-βRⅡ的表达载体 pcDNA3.1(+)/sTGF-βRⅡ,转染人大肠癌 LoVo 细胞,以逆转录-聚合酶链反应(RT-PCR)、Western blot 和免疫荧光技术分别检测目的基因及其蛋白表达。噻唑蓝(MTT)比色法和平板法集落形成实验检测 sTGF-βRⅡ对LoVo 细胞增殖的影响,Annexin V-FITC 和 PI 双染流式细胞术检测 sTGF-βRⅡ对 LoVo 细胞凋亡的影响,Matrigel^(TM)侵袭试剂盒检测 sTGF-βRⅡ对 LoVo 细胞侵袭能力的影响。结果 sTGF-βRⅡ在LoVo 细胞中获得表达。sTGF-βRⅡ可抑制 TGF-β1作用下的 LoVo 细胞增殖、凋亡和体外侵袭能力。结论 sTGF-βRⅡ可拮抗 TGF-β1的信号传导,对大肠癌 LoVo 细胞的侵袭能力具有一定的抑制作用,在大肠癌的治疗中可能有一定的作用。 | 周瑞 熊斌 王新波 | 2007 | 中华实验外科杂志2007，24，12： | 3 |
| 13 | 转化生长因子β1及丁酸钠对大肠癌细胞增殖的影响显示文摘目的:研究转化生长因子β1(TGF-β1)及丁酸钠对大肠癌HCT-116细胞体外增殖的影响,并探讨其可能的机制。方法:采用MTT比色法观察两药对大肠癌细胞增殖的抑制,ELISA检测其对尿激酶系统的影响,流式细胞仪(FCM)分析其对细胞周期的影响。结果:MTT显示TGF-β1与丁酸钠均能抑制HCT-116细胞的增殖,呈浓度和时间依赖性(P<0.05);两药联用可使其作用不同程度地增强。FCM显示两药均能使G1期细胞比例升高,与对照组比较差异均有显著性(P<0.05);两药联用可使其作用不同程度的增强。TGF-β1能够上调UPA活性和PAI-1水平(P<0.05),丁酸钠则抑制UPA活性(P<0.05),对PAI-1水平无明显影响。丁酸钠可部分抑制TGF-β1引起的UPA活性的增加(P<0.05),而对PAI-1无明显影响。结论:TGF-β1与丁酸钠均可以抑制大肠癌HCT-116细胞的增殖及阻止细胞周期的进展,两者联用具有协同作用;同时TGF-β1与丁酸钠可通过调节尿激酶系统影响大肠癌细胞的增殖与浸润。 | 王朝晖 张倩洁 曹先乾 | 2012 | 实用医学杂志2012，28，18： | 3 |
| 14 | 胃癌病人血清与癌组织nm23H1蛋白表达及临床病理意义显示文摘目的研究胃癌病人血清、癌组织中nm23H1蛋白的表达及与临床病理特征的关系。方法采用ELISA法及免疫组织化学S-P法,检测69例胃癌病人血清、癌组织中nm23H1蛋白的表达情况,并分析其与临床病理特征的关系。结果胃癌病人血清、癌组织中nm23H1蛋白的表达与正常对照组比较差异有显著性(χ2=9.69,t=6.96,P<0.05)。胃癌病人血清、癌组织中nm23H1蛋白的表达与淋巴结转移及肿瘤临床分期有关(χ2=12.30、5.30,t=14.67、6.42,P<0.05)。结论胃癌病人血清及癌组织中nm23H1蛋白的表达下调,nm23H1蛋白可以作为评估胃癌病人预后的指标。 | 宋培铎 洪光晨 | 2014 | 齐鲁医学杂志2014，29，1： | 3 |
| 15 | 拉威尔钢琴作品的表演艺术显示文摘本文将从拉威尔的教育和生活的经历等多个对他创作产生重要影响的时代背景入手,对拉威尔各个时期作品的创作特点及风格进行分析,在作品独特的和声、对位、结构、节奏、作者标注等细节中,着重研究如何正确的读谱即读出乐谱背后的真正含义,总结表现音乐所需要的技巧,如:触键方法、踏板用法、节奏和速度的处理,具体的归纳演奏拉威尔作品的要点和难点。在拉威尔一贯坚持的'创新精神'和'音乐本色'的理念基础之上,挖掘出钢琴表演艺术中更大的可能性,阐述有别于古典时期、浪漫主义时期及现代派作品的拉威尔钢琴作品的演奏特点。 | 崔岚 | 2014 | 乐府新声(沈阳音乐学院学报)2014，32，1： | 2 |
| 16 | 结直肠癌肿瘤标记物研究现状显示文摘 | 杨华 肖刚 | 2007 | 结直肠肛门外科2007，13，4： | 2 |
| 17 | 转化生长因子β1和转化生长因子βⅡ受体蛋白在口腔鳞状细胞癌中的表达显示文摘目的 :研究口腔鳞状细胞癌组织中TGFβ1和TGFβRⅡ的表达及其临床意义。 方法 :用链霉素亲生物素 过氧化酶免疫组织化学 (SP)法检测 4 0例口腔鳞状细胞癌组织中TGFβ1和TGFβRⅡ的表达 ,分析TGFβ1和TGFβRⅡ的表达与临床病理参数的关系。结果 :TGFβ1表达阳性率为 5 5 % (2 2 / 4 0 ) ,TGFβRⅡ表达阳性率为 6 0 % (2 4 / 4 0 )。TGFβ1和TGFβRⅡ的表达与口腔鳞状细胞癌患者的年龄、性别及组织分化程度无明显关系 (P >0 .0 5 ) ,与临床分期和淋巴结转移有关。Ⅲ +Ⅳ期与Ⅰ +Ⅱ期比较 ,原发灶中TGFβ1表达率有升高趋势、TGFβRⅡ表达率有降低趋势(P <0 .0 5 )。有淋巴结转移者与无淋巴结转移者比较 ,原发灶中TGFβ1表达率有升高趋势、TGFβRⅡ表达率有降低趋势 (P <0 .0 5 )。结论 :TGFβ1和TGFβRⅡ的表达与口腔鳞状细胞癌的增殖、浸润和转移有关 ,检测口腔鳞状细胞癌中TGFβ1和TGFβRⅡ的表达有助于临床诊断和治疗。 | 孙桃姣 朱友家 陈建刚 龚玲玲 | 2004 | 武汉大学学报(医学版)2004，25，5： | 2 |
| 18 | TGF-β_1和CEA在大肠癌组织中的表达及其意义显示文摘目的 检测癌胚抗原 (CEA)和转化生长因子 β1(TGF β1)在大肠癌组织中的表达 ,结合临床病理资料探讨其相关意义。方法 应用免疫组织化学方法检测大肠癌、大肠腺瘤、癌近旁粘膜及正常粘膜中CEA、TGF - β1表达情况。结果 CEA、TGF β1在大肠癌中的阳性表达率分别为 74 19%和 6 9 35 %,明显高于两者在大肠腺瘤、正常粘膜中的表达 (P <0 0 5 ,P <0 0 1) ,同时癌近旁粘膜中两者的表达高于正常粘膜 (P <0 0 5 ) ;CEA和TGF β1在大肠癌各组间表达无统计学差异 ;DukesA +B期中两者的表达低于DukesC +D期 (P <0 0 5 ) ;两者在大肠癌中的表达有相关性 (P <0 0 1)。结论 大肠癌能同时分泌CEA、TGF β1,两者的过度表达与大肠癌发生、发展、Dukes分期、癌组织浸润、转移范围有关 ;CEA、TGF - β1在大肠癌各组间表达无明显差异 ;少部分大肠癌近旁粘膜已偏离正常细胞生长状态 ,具有肿瘤细胞生长代谢的某些特征。 | 马颖 李冠群 | 2003 | 辽宁医学杂志2003，17，3： | 2 |
| 19 | 转化生长因子β_1在食管鳞癌组织中的表达及临床意义显示文摘目的通过检测食管鳞癌组织标本及癌旁正常组织标本中转化生长因子β1(TGF-β1)的水平,探讨TGF-β1与食管鳞癌发生发展之间的关系。方法收集2009年9月~2011年10月沈阳医学院奉天医院胸外科39例食管鳞癌组织标本及癌旁正常组织标本,用逆转录聚合酶链反应的方法对标本进行检测,比较食管鳞癌组织及癌旁正常组织TGF-β1信使RNA(TGF-β1mRNA)的表达水平。结果食管鳞癌组织中TGF-β1mRNA水平(0.76±0.09)显著高于癌旁正常组织(0.23±0.05);而随着病理分期Ⅰ~Ⅲ期TGF-β1mRNA水平逐渐增高(Ⅰ期:0.52±0.06;Ⅱ期:0.73±0.03;Ⅲ期:0.92±0.05)。有淋巴结转移组的TGF-β1mRNA水平(0.91±0.06)显著高于无淋巴结转移组(0.55±0.08)。随着肿瘤细胞局部浸润深度增加,TGF-β1mRNA水平逐渐增高(T1:0.53±0.03;T2:0.69±0.05;T3:0.93±0.03)。结论 TGF-β1水平的增高与食管鳞癌的发生发展关系密切。 | 王泽学 周志明 梁磊 | 2013 | 中国医药导报2013，10，17： | 1 |
| 20 | 转化生长因子βⅡ型受体在胃癌组织中表达的研究显示文摘目的:研究转化生长因子β(TGFβ)Ⅱ型受体在胃癌组织中的表达及其意义. 方法:应用免疫组织化学方法,对20例正常胃粘组织及74 例胃癌组织(高中分化腺癌32例,低分化腺癌23例,黏液腺癌14例,印戒细胞癌5例)TGFβⅡ型受体的表达情况进行了研究. 结果:在正常胃黏膜组织,TGFβⅡ型受体主要表达于腺体的体底部;在胃高中分化腺癌组织中,TGFβⅡ型受体表达减少(P<0.05,vs 正常胃黏膜);在低分化腺癌及黏液印戒细胞癌中,其表达明显减少(P均<0.01,vs 正常胃黏膜). 结论:TGFβⅡ型受体表达的减少可能在胃癌的发生发展中起了一定的作用. | 张朋彬 李宜辉 徐采朴 | 2004 | 世界华人消化杂志2004，12，7： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员