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| 1 | Dev Cell:科学家阐明人类胚胎原生殖细胞发育的分子机制显示文摘近日,刊登于国际杂志Developmental Cell上的一项研究报道中,来自巴布拉汉研究所(Babraham Insdtute)的研究人员通过研究调查了原生殖细胞发育的早期阶段,同时他们在实验室开发了一种产生类似细胞的策略,这种酷似人类原始生殖细胞的产生对于未来生殖领域的研究,以及分析人类潜在的继代基因调控的遗传机制非常重要。 | Ferdinand von Meyenn Rebecca V. Berrens Simon Andrews Fátima Santos Amanda J. Collier Felix Krueger Rodrigo Osorno Wendy Dean Peter J. Rugg-Gunn Wolf Reik | 2016 | 现代生物医学进展2016，16，35： | 2 |
| 2 | Microcalorimetric study of a proposed test reaction—the imidazole catalysed hydrolysis of triacetin显示文摘 | Andrew K. Hills Anthony E. Beezer Joseph A. Connor John C. Mitchell Gert Wolf Felix Baitalow | 2002 | Thermochimica Acta2002，，2： | 1 |
| 3 | The Scalasca performance toolset architecture显示文摘 | Markus Geimer Felix Wolf Brian J N | 2010 | Concurrency and Computation:Practice and Experience2010，22，6： | 1 |
| 4 | Structral paramters of collagen nerve grafts influence peripheral nerve regeneration显示文摘 | Felix Stang Hisham Fanss Gerald Wolf | 2005 | Biomaterials2005，26，16： | 1 |
| 5 | Bio-compatibility of type Ⅰ/Ⅲ collagen matrix for peripheral nerve reconstruction显示文摘 | Felix Stang Gerald Wolf | 2003 | Biomaterials2003，24，： | 1 |
| 6 | Survey of conventional and spiral CT doses显示文摘 | Wolf M Nunnemann A Schroder RJ Felix R | 2001 | Radiology2001，218，： | 1 |
| 7 | The Scalasca performance toolset architecture 显示文摘 | Markus Geimer Felix Wolf Brian J N | 2010 | Concurrency and Computation: Practice and Experience2010，22，6： | 1 |
| 8 | Conventional and rapid MR imaging of the liver with Gd- DTPA 显示文摘 | Hamm B Wolf KJ Felix R | 1987 | Radiology1987，164，2： | 1 |
| 9 | Structural parameters of collagen nerve grafts influence peripheral nerve regeneration显示文摘 | Hisham Fansa Gerald Wolf | 2005 | Biomaterials2005，26，16： | 1 |
| 10 | finDr:A web server for in silico D-peptide ligand identification显示文摘In the rapidly expanding field of peptide therapeutics,the short in vivo half-life of peptides represents a considerable limitation for drug action.D-peptides,consisting entirely of the dextrorotatory enantiomers of naturally occurring levorotatory amino acids(AAs),do not suffer from these shortcomings as they are intrinsically resistant to proteolytic degradation,resulting in a favourable pharmacokinetic profile.To experimentally identify D-peptide binders to interesting therapeutic targets,so-called mirror-image phage display is typically performed,whereby the target is synthesized in D-form and L-peptide binders are screened as in conventional phage display.This technique is extremely powerful,but it requires the synthesis of the target in D-form,which is challenging for large proteins.Here we present finDr,a novel web server for the computational identification and optimization of D-peptide ligands to any protein structure(http://gffzz4d57a956f0c348acskvxpoqpbf0pq6wbk.ffgz.tsg.suse.edu.cn/).finDr performs molecular docking to virtually screen a library of helical 12-mer peptides extracted from the RCSB Protein Data Bank(PDB)for their ability to bind to the target.In a separate,heuristic approach to search the chemical space of 12-mer peptides,finDr executes a customizable evolutionary algorithm(EA)for the de novo identification or optimization of D-peptide ligands.As a proof of principle,we demonstrate the validity of our approach to predict optimal binders to the pharmacologically relevant target phenol soluble modulin alpha 3(PSMα3),a toxin of methicillin-resistant Staphylococcus aureus(MRSA).We validate the predictions using in vitro binding assays,supporting the success of this approach.Compared to conventional methods,finDr provides a low cost and easy-to-use alternative for the identification of D-peptide ligands against protein targets of choice without size limitation.We believe finDr will facilitate D-peptide discovery with implications in biotechnology and biomedicine. | Helena Engel Felix Guischard Fabian Krause Janina Nandy Paulina Kaas Nico Hofflin Maja Kohn Normann Kilb Karsten Voigt Steffen Wolf Tahira Aslan Fabian Baezner Salom´e Hahne Carolin Ruckes Joshua Weygant Alisa Zinina Emir Bora Akmeriç Enoch B.Antwi Dennis Dombrovskij Philipp Franke Klara L.Lesch Niklas Vesper Daniel Weis Nicole Gensch Barbara Di Ventura Mehmet Ali Oztürk | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 0 |
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