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| 1 | Combination of epidural electrical stimulation with ex vivo triple gene therapy for spinal cord injury:a proof of principle study显示文摘Despite emerging contemporary biotechnological methods such as gene-and stem cell-based therapy,there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury.Our previous studies have demonstrated that transplantation of genetically engineered human umbilical cord blood mononuclear cells producing three recombinant therapeutic molecules,including vascular endothelial growth factor(VEGF),glial cell-line derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)can improve morpho-functional recovery of injured spinal cord in rats and mini-pigs.To investigate the efficacy of human umbilical cord blood mononuclear cells-mediated triple-gene therapy combined with epidural electrical stimulation in the treatment of spinal cord injury,in this study,rats with moderate spinal cord contusion injury were intrathecally infused with human umbilical cord blood mononuclear cells expressing recombinant genes VEGF165,GDNF,NCAM1 at 4 hours after spinal cord injury.Three days after injury,epidural stimulations were given simultaneously above the lesion site at C5(to stimulate the cervical network related to forelimb functions)and below the lesion site at L2(to activate the central pattern generators)every other day for 4 weeks.Rats subjected to the combined treatment showed a limited functional improvement of the knee joint,high preservation of muscle fiber area in tibialis anterior muscle and increased H/M ratio in gastrocnemius muscle 30 days after spinal cord injury.However,beneficial cellular outcomes such as reduced apoptosis and increased sparing of the gray and white matters,and enhanced expression of heat shock and synaptic proteins were found in rats with spinal cord injury subjected to the combined epidural electrical stimulation with gene therapy.This study presents the first proof of principle study of combination of the multisite epidural electrical stimulation with ex vivo triple gene therapy(VEGF,GDNF and NCAM)for treatment of spinal cord injury in rat models.The animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.2.20.02.18)on February 20,2018. | Filip Olegovich Fadeev Farid Vagizovich Bashirov Vahe Arshaluysovich Markosyan Andrey Alexandrovich Izmailov Tatyana Vyacheslavovna Povysheva Mikhail Evgenyevich Sokolov Maxim Sergeevich Kuznetsov Anton Alexandrovich Eremeev Ilnur Ildusovich Salafutdinov Albert Anatolyevich Rizvanov Hyun Joon Lee Rustem Robertovich Islamov | 2021 | Neural Regeneration Research2021，16，3： | 4 |
| 2 | Opportunities for ionic liquids in recovery of biofuels 显示文摘 | Fadeev A G Meagher M M | 2001 | Chem Comm2001，，29： | 1 |
| 3 | Ultrahydrophobic and ultralyophobic surfaces:some comments and examples显示文摘 | Chen W Fadeev A Y Hsieh M C | 1999 | Langmuir1999，15，10： | 1 |
| 4 | Ultrahydrophobic and ultralyophobic surfaces:some comments and examples显示文摘 | CHEN W FADEEV A Y HSIEH M C | 1999 | Langmuir1999，15，10： | 1 |
| 5 | Mitochondrial DNA data imply a stepping-stone colonizaiton of Beringia by arctic warbler Phylloscopus borealis显示文摘 | Reeves AB Drovetski SV Fadeev V | | 0，，： | 1 |
| 6 | Self-assembly is not the only reac-tion possible between alkyltrichlorosilanes and surfaces:monomolecu-lar and oligomerie eovalently attached layers of diehloro-and trichlo-roalkylsilanes on silicon显示文摘 | FADEEV A Y MCCARTHY T J | 2000 | Langmuir2000，16，18： | 1 |
| 7 | Ultra-hydrophobic and ultra-hydrophobic surfaces: some comment s and examples显示文摘 | Chen W Fadeev AY Hsieh MC | 1999 | Langmuir1999，15，10： | 1 |
| 8 | Uhrahydrophobie and ultralyophobic surfaces: some comments and examples显示文摘 | CHEN W FADEEV A Y HSIEH M C | 1999 | Langmuir1999，15，10： | 1 |
| 9 | Biochemistry (Moscow) Supplement Series A显示文摘 | FADEEV A S YAMPOLSKAYA G P LEVACHEV S M | 2008 | Membrane and Cell Biology2008，，2： | 1 |
| 10 | Perlpartum anesthetic management of patients with Takayasu' s arteritis : ease series and review 显示文摘 | Ioseovieh A Gislason R Fadeev A | 2008 | Int J Obstet Anesth2008，17，4： | 1 |
| 11 | Properties of Spreaded Collagen I Monolayers on the Surface of Aqueous tert-Butanol and n-Hexanol So- lutions 显示文摘 | FADEEV A S YAMPOLSKAYA G P RUDOY V M | 2003 | Colloid J2003，65，： | 1 |
| 12 | Feynman diagrams for the Yang-Mills field显示文摘 | FADEEV L D POPOV V N | 1967 | Phys Lett1967，25，1： | 1 |
| 13 | Use of Ionic Liquids for w -Conjugated Polymer Electrochemical Devices显示文摘 | Lu W Fadeev A G Qi B | 2002 | Science2002，297，： | 1 |
| 14 | Opportunities for ionic liquids inrecovery of biofuels显示文摘 | FADEEV A G MEAGHER M M | 2001 | Chem Commun2001，3，： | 1 |
| 15 | Covalently Attached Monolayers of Oligo(dimethylsiloxane)s on Silica: A Siloxane Chemistry Approach for Surface Modification显示文摘 | Alexander Y Fadeev Yuri V Kazakevich | 2002 | Langmuir 20022002，，： | 1 |
| 16 | U ltrahydrophobic and uhralyophobic surfaces: some comments and examples 显示文摘 | Chen W Fadeev A H sieh M | 1999 | Langmuir1999，15，10： | 1 |
| 17 | Self-assembly is not the only reaction possible between alkyltrichlorosilanes and surfaces: monomolecular and oligomeric covalently attached layers of dichloro-and trichloroalkylsilanes on silicon 显示文摘 | Fadeev Alexander Y Mccarthy Thomas J | 2000 | Langmuir2000，16，18： | 1 |
| 18 | Chem Commun显示文摘 | Fadeev A G Meagpher M M | 2001 | 295-2962001，，： | 1 |
| 19 | Valence band of liquid water Raman scattering: some peculiarities and applications in the diagnostics of water media显示文摘 | Dolenko T.A Churina I.V Fadeev V.V | | 0，，8： | 1 |
| 20 | Uhrahydrophobic and uhralyophobic surfaces: some comments and examples 显示文摘 | CHEN W FADEEV A Y HSIEH M C | 1999 | Langmuir1999，15，： | 1 |
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