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| 1 | RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis )in Chinese coastal waters of the Huanghai Sea and coastal waters of the Bohai Sea显示文摘Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each individual. The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty--nine of 106 loci detected were polymorphic, amounting to 36. 8 %. Mean genetic distance was 0. 094 1 0. 020 6; 68 markers (63. 2 % of the total) showed stable homogeneity in all of the 32 individuals. | Liu Ping Kong Jie Shi Tuo Zhuang Zhimeng Deng Jingyao (l. Huanghai Sea Fisheries Research institute , Chinese Academy of Fishery Sciences, Qingdao 266071, China) | 2000 | Acta Oceanologica Sinica2000，19，1： | 18 |
| 2 | 青鱼性腺发育的研究显示文摘湖南地区生长于池塘环境的青鱼,性成熟年龄是5—6年,雄性比雌性普遍地早熟一年。卵母细胞和滤泡细胞是同源的,都来自于卵原细胞。池养青鱼的卵母细胞只能发育到初级卵母细胞阶段(Ⅳ时相),必须通过人工催情,才能进行染色体的减数分裂,使卵母细胞由第Ⅳ时相发育到第Ⅴ时相。精细胞的发生,能够完成由精原细胞到精子的全部发育过程。青鱼在第一次性周期内,雄性精巢在第5个冬季进入第Ⅳ期,雌性卵巢在第6个冬季进入第Ⅲ期,从此以后,每年冬季,雄性精巢回复到第Ⅳ期,雌性卵巢回复到第Ⅲ期,这种性腺季节周期变化的规律,为生产上选留亲鱼提供了理论依据。青鱼雌性卵母细胞由第Ⅲ时相到第Ⅳ时相是同步性的;经人工催情产卵或自然退化后,卵巢的组织学结构又回复到第Ⅱ期,证明青鱼是一次产卵类型。已经达到性成熟年龄的雌性青鱼,卵母细胞的卵黄形成有两种不同的类型。第一种类型是泡内卵黄,第二种类型是泡外卵黄。如果饲养管理工作如投饵、水质调节不适宜,卵母细胞不能正常形成卵黄,就会出现卵子的败育现象,这是生产上一个重要问题,必须进一步研究。 | Fishery Research Group,Department of Biology,Hunan Teachers’ College | 1975 | 水生生物学集刊1975，5，4： | 13 |
| 3 | The single nucleotide polymorphisms of chicken melanocortin-4 receptor(MC4R)gene and their association analysis with carcass traits显示文摘Mutations of the melanocortin-4 receptor (MC4R) gene are associated with the appetite,obesity and growth in pig,mice and human. But little is known about the function of chicken MC4R gene. In this study,F2 chicken resource population derived from broilers crossing to Silky was screened for the polymorphisms of the MC4R gene using PCR-single strand conformation polymor-phism (PCR-SSCP) and DNA sequencing methods. Four single nucleotide polymorphisms (SNPs) sites were found. The mutation (C→T) in the 5′ regulation region of chicken MC4R gene results in one more NF-E2 and cap transcription factor binding sites in the mutation allele than in the wild allele. One missense mutation (G→A) occurs in the coding region (61nt),which changes the glycine to arginine. Moreover,in the coding region there are 2 synonymous mutations,one G→T mutation at 315nt and one C→T mutation at 336nt. Least square analysis of the SNPs and carcass traits showed that BB,DD and FF genotypes are significantly associated with body weight,carcass weight (or half carcass weight),and leg muscle weight (P<0.05 or P<0.01). But no significant association between the geno-types and abdominal fat weight is found. The results present the evidence that the chicken MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight and growth. | QIU Xuemei1,2,LI Ning1,DENG Xuemei3,ZHAO Xingbo3,MENG Qingyong1 & WANG Xiuli2 1. State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China 2. College of Life Science and Technology,Dalian Fisheries University,Dalian,116023,China 3. College of Animal Science and Technology,China Agricultural University,Beijing 100094,China | 2006 | Science China(Life Sciences)2006，49，6： | 12 |
| 4 | Environmental Implications of Excessive Selenium: A Review显示文摘Selenium is a naturally occurring trace element that is nutritionally required in smallamounts but it can become toxic at concentrations only twice those required. The narrow mar-gin between beneficial and harmful levels has important implications for human activities thatincrease the amount of selenium in the environment. Two of these activities, disposal of fbssilfuel wastes and agricultural irrigation of arid, seleniferous soils, have poisoned fish andwildlife, and threatened public health at several locations in the United States. Research stud-ies of these episodes have generated a data base that clearly illustrates the environmental hazardof excessive selenium. It is strongly bioaccumulated by aquatic organisms and even slight in-creases in waterborne concentrations can quickly result in toxic effects such as deformed em-bryos and reproductive failure in wildlife. The selenium data base has been very beneficial indeveloping hazard assessment procedures and establishing environmentally sound water qualitycriteria. The two faces of selenium, required nutrient and Potent toxin, make it a particularlyimportant trace element in the health of both animals and man. Because of this paradox, envi-ronmental selenium in relation to agriculture, fisheries, and wildlife wiIl continue to raise im-POrtant land and water management issues for decades to come. If these issues are dealt withusing prudence and the available environmental selenium data base, adverse irnpacts to naturalresources andspublic health can be | A. DENNIS LEMLY(United States Forest Service, Southern Research Station, Coldwater Fisheries Research Unit, Department of Fisheries and Wildlife Sciences, Virginia Tech University, Blacksburg, VA 24061-0321, USA) | 1997 | Biomedical and Environmental Sciences1997，10，4： | 10 |
| 5 | Establishment of trophic continuum in the food web of the Yellow Sea and East China Sea ecosystem: Insight from carbon and nitrogen stable isotopes显示文摘Stable carbon and nitrogen isotope ratios (δ13C and δ15N) are used to study the trophic structure of food web in the Yellow Sea and East China Sea ecosystem. The trophic con-tinuum of pelagic food web from phytoplankton to top preyer was elementarily established, and a trophic structure diagram in the Yellow Sea and East China Sea was outlined in combination with carbon isotopic data of benthic organisms, which is basically consistent with and makes some improvements on the simplified Yellow Sea food web and the trophic structure diagram drawn based on the biomass of main resource population during 1985―1986. This result indicates that the stable isotope method is a potential useful means for further studying the complete marine food web trophic continuum from viruses to top predators and food web stability. | CAI Deling1, LI Hongyan2, TANG Qisheng3 & SUN Yao3 1. Key Laboratory of Marine Sedimentology & Environment Geology, SOA, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2. Ocean University of China, Qingdao 266003, China 3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | 2005 | Science China(Life Sciences)2005，48，6： | 8 |
| 6 | Identification of Porphyra lines using computerized DNA fingerprinting显示文摘RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P. katadai var. Hemiphylla and P. digospermatangia). Eight stable and repeatable RAPD bands amplified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprinting. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting. It can be used in practical Porphyra line identification. | WangBin, Jia Jianhang, Shi Jinfeng, Chen Yihua, JinDemin, Xu Pu, Mei Junxue, Weng Manli 1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 (E-mail: mlweng@genetics. ac. cn), China 2. Jiangsu Marine Fishery Institute, Nan | 2001 | Acta Oceanologica Sinica2001，20，3： | 7 |
| 7 | Analysis of tandem repeats in the genome of Chinese shrimp Fenneropenaeus chinensis显示文摘Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of Chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2159 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 20.6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2% of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42%. In decreasing order, the 20 most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (274), AAT (92), A (56), AAG (28), ATC (27), ATAG (27), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them. | KONG Jie1 & GAO Huan2,3 1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Chinese Science Bulletin2005，50，14： | 7 |
| 8 | The acoustic survey of anchovy in the Yellow Sea in February 1999, with emphasis on the estimation of the size structure of the anchovy population显示文摘Estimation of the size structure of a fish population is often one of the primary objectives of an acoustic/trawl survey.The methods of estimation may be different with different sampling strategies.This paper describes the main results as well as the methods used during the acoustic survey of anchovy in the Yellow Sea in February 1999,with emphasis on the estimation of the number at length and biomass at length of the anchovy population surveyed under adoptive sampling strategy.The estimation is done for each transect.Within each transect,the biological sample data is weighted by fish quantity derived from the acoustic density ( s A) of the echo signs that the biological sample represents.This method makes full use of the acoustic data available in a rigorous manner.It is essentially to estimate the size structure of each group of fish aggregation thought to having similar size composition.It is believed that this method may lead to a more accurate estimate of the size structure of a fish population surveyed under adoptive sampling strategy. | Zhao Xianyong Yellow Sea Fisheries Research Institute,106 Nanjing Road,Qingdao, China 266071 | 2001 | 海洋水产研究2001，22，4： | 7 |
| 9 | Preliminary analysis of distribution and variation of perennialmonthly mean water masses in the Bohai Sea,the Huanghai Sea and the East China Sea显示文摘On the basis of perennial monthly mean temperature and salinity data, the classification of monthly water masses at the surface and the bottom in the Bohai Sea, the Huanghai Sea and the East China Sea, has been made by using the method of fuzzy cluster from the modified characteristic of water masses in the shallow water area. In this paper, the basic features, growth and decline patterns of water masses in relation to fishing grounds in the whole shelves of the Bohai Sea, the Huanghai Sea and the East China Sea are discussed with emphasis. | Liu Shuxun, Shen Xinqiang, Wang Youqin and Han Shixin 1. East China Sea Fisheries Research Institute, No. 300, Jungong Road, Shanghai 200090, China | 1992 | Acta Oceanologica Sinica1992，11，4： | 6 |
| 10 | Kuroshio warm filament and the source of the warm water of the Tsushima Current显示文摘Characteristics and evolution of the Kuroshio frontal eddies and warm filaments are analyzed according to two series of satellite images (March 5 to 7, 1986 and April 14 to 16, 1988). The results show that the frontal eddies in the East China Sea are generated at the shelf break and move along the continental slope at a speed of 15 cm/s with the Kuroshio. The frontal eddies occur about every 10 d and evolve to be warm filaments a few hundred km in length and 30-40 km in width in the area west of the Yaku-shima. Meanwhile, the existence of the warm filament was also found in the area by analysing the hydrographic data in the area west of Kyushu during May 24-June 5, 1988.The Kuroshio warm filaments move westward opposite to the Kuroshio and then turn northward at the shelf break and become the main source of the warm water of the Tsushima Warm Current. A simple dynamic explanation for the process is presented in this paper. | Guo Binghuo, Xiu Shumeng, Haruo Ishii and Yasuaki Nakamura First Institute of Oceanography, State Oceanic Administration, Oingdao, 266003, ChinaHydrographic Department, Maritime Safety Agency, Tokyo, JapanSeikai Fisheries Research Institute, Fisheries Agency Nagasaki, Japan | 1991 | Acta Oceanologica Sinica1991，10，3： | 6 |
| 11 | 广西鲮鱼的生物学及其养殖显示文摘本文对鲮鱼的食性、年龄、生长和繁殖进行了研究,并对渔业生产提出了建议。鲮鱼以浮游植物为主要食料。在水温14.6—29.4℃时食欲旺盛。当水中溶氧量0.24—0.65毫克/升时,绝大部分个体停止摄食,而溶氧量在0.99毫克/升以上时,摄食强度很大。见到的最大个体:雌鱼体长595毫米,体重4,100克,10龄;雄鱼体长580毫米,体重3,850克,9龄。鲮鱼能在大王滩水库中自然繁殖。鲮鱼人工繁殖季节以5月初至6月中旬较适宜;催产时水温以22—29℃为好,注射催产剂的效应时间一般为4.5—6小时。见到的性成熟最小个体:郁江鲮鱼雌体长273毫米,体重525克,雄体长275毫米,体重415克;池养鲮鱼雌体长247毫米,体重366克,雄体长235毫米,体重291克。根据群众经验,一般池塘中鲮鱼每亩放养1,000尾,鲮与鲢的放养比例以3∶1较好,以鲮鱼为主体的池塘则每亩放养1,500—2,000尾较好。 | The Fisheries Research Institute of the Kwangsi Chuang Autonomous Region | 1975 | 水生生物学集刊1975，5，4： | 6 |
| 12 | Application of RAPD technology for identification in three different stocks of Penaeus chinensis显示文摘The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coastal waters of the Korea Peninsula, H was from the Huanghai Sea—Bohai Sea stock and S was from the health cultured stock in Jimo, Shandong Province, China. Three genetic markers, F-02 540, OPN-13 800 and OPN-20 2200, which could differentiate the three different stocks, have been reported. The basic data for marker assisted selection (MAS) on genetic breeding of Penaeus chinensis are provided. | Liu Zhenhui1, Kong Jie 2, Liu Ping2, Deng Jingyao2 1. College of Marine Biology Science, Ocean University of China, Qingdao 266003, China 2. Huanghai Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China | 2003 | Acta Oceanologica Sinica2003，22，4： | 5 |
| 13 | Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean显示文摘A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 ℃ and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 ℃ and 7.5 ℃ respectively. At 0℃, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 ℃ for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu 2+ and Zn 2+. | Lin Xuezheng1,Yang Xiuxia2,Bian Ji1,Huang Xiaohang1 1. First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China 2. Fishery College, Ocean University of China,Qingdao 266003,China | 2003 | Acta Oceanologica Sinica2003，22，4： | 5 |
| 14 | Study on purification and ultrastructure of a baculovirus in Penaeus chinensis显示文摘 | Shi Tuo, Kong Jie, Bao Zhenmin, Liu Ping and Wang Chongming (Huanghai Sea Fisheries Research Institute, Qingdao 266071, China)Liang Xingming, (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China) | 1998 | Acta Oceanologica Sinica1998，17，4： | 4 |
| 15 | Primary Screening of 10-Hydroxy-2-Decenoic Acid Productive Strains显示文摘In this paper, eleven strains, which were screened strictly from raw royal jelly, soil andhoneycomb etc. by meansof dilution plate and spread plate methods, were cultured at 28℃ for 60 h with shaking. To determine whether they could yield10-Hydroxy-2-decenoic acid during fermentation, gas chromatography and gas chromatography-mass spectrometry methodswere used. The results showed that the strains BH002 and BH004, were both identified as Crytococcaceae, where BH002was primarily classified into Candida for possessing the abilities. The 10-HDA productivity of Candida BH002 and that ofBH004 were 0.327% and 0.2648% respectively. | Bailin Li Yongxin Kang Lingjing Wei Lizhen HuangFaculty of Food Science & Technology Shanghai Fisheries University Shanghai 200090 | 2002 | 食品科学2002，23，8： | 4 |
| 16 | 龙窝湖斜颌鲴生态调查报告显示文摘斜颌鲴是一种重要的野生经济鱼类,分布于我国黑龙江、长江、珠江流域(伍献文等。《中国鲤科鱼类志》上卷)。 | Fisheries Administrative Board of Lake Lungwohu,Fanchang County,Anhwei Province Fisheries Administrative Station of Wuhu District,Anhwei Province Section of Introduction and Acclimation,the 2nd Laberatory,Institute of Hydrobiology,Hupei Province | 1977 | 水生生物学集刊1977，6，2： | 4 |
| 17 | Hepatic Histopathological Characteristics and Antioxidant Response of Phytoplanktivorous Silver Carp Intraperitoneally Injected with Extracted Microcystins显示文摘Objective To investigate the hispathological characteristics and antioxidant responses in liver of silver carp after intraperitoneal administration of microcystins (MCs) for further understanding hepatic intoxication and antioxidation mechanism in fish. Methods Phytoplanktivorous silver carp was injected intraperitoneally (i.p.) with extracted hepatotoxic microcystins (mainly MC-RR and -LR) at a dose of 1000 μg MC-LReq./kg body weight, and liver histopathological changes and antioxidant responses were studied at 1, 3, 12, 24, and 48 h, respectively, after injection. Results The damage to liver structure and the activities of hepatic antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxide (GPX) were increased in a time-dependent manner. Conclusion In terms of clinical and histological signs of intoxication and LD50 (i.p.) dose of MC-LR, silver carp appears rather resistant to MCs exposure than other fishes. Also, the significantly increased SOD activity in the liver of silver carp suggests a higher degree of response to MCs exposure than CAT and GPX. | LI LI AND PING XIE Fisheries College of Huazhong Agricultural University, Wuhan 430070, Hubei, China Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China | 2009 | Biomedical and Environmental Sciences2009，22，4： | 4 |
| 18 | Chemical induction of settlement and metamorphosis in two Babylonia (Gastropoda) larvae显示文摘By exposure to 12 x 10 3 mol/dm3 K+ for 24 h, more than 90% of planktonic larvae of Babylonia formosae and B. areolata were effectively induced to metamorphose completely. 15 x 10 - 3 mol/dm3 K+ exerted toxic effect on larval B. areolata over a 24-h period, but produced better results of induction by 12 h exposure. Metamorphosed juveniles induced by increased by K’ could move and feed normally. Percent metamorphosis increased with average shell height for K + -induced larvae. L-DOPA, epinephrine and norepinephrine ranging from 5. 5 x 10 -- 6 to 5. 5 x 10 - 5 mol/dm3 had no inductive activity to metamorphosis in larval B. areolata, whereas the rate of metamorphosis induced by dopamine at a concentration of 10--4 mol/dm3 was approximately half less than that by 12 x 10 3 mol/dm3 K+. TEA (a K+ -channel blocker) did not inhibit metamorphosis in the two Babylonia larvae by induction of K+. Based on the results of K+ -induced experiments, it is preliminarily suggested that shell height of larvae achieving metamorphic competence was in the range of 860. 2 - 1 009. 8μm for B. formosae, and 849. 6- 956. 0 um for B. areolata. In addition, planktonic larvae of the two Babylonia had delayed metamorphosis. | Ke Caihuan Li Shaojing Li Fuxue Zheng Zhon Liu Weibin (1. Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China 2. Fujian Fisheries Research Institute, Xiamen 361012, China) | 2000 | Acta Oceanologica Sinica2000，19，1： | 4 |
| 19 | Fish species diversity in the Huanghe Estuary显示文摘This paper lists 50 fish specs in the Huanghe Estuary, which belong to 9 orders, 22 families and 37 genera. The paper describes the seasonal distributions of these fish species and the succession of the important fish species during the past 50 a. The authors discuss the main factors that have resulted in the changes in fish species diversity and the fishery resources. These include the overexploitation of the fishery and the sharp reduction in the fresh water discharges from the Huanghe River. | Chen Dagang, Jiao Yan, Liu Qun, Zeng Xiaoqi, Ren Yiping (Department of Fisheries,Ocean University of Qingdao, Qingdao 266003, China.) | 2000 | Acta Oceanologica Sinica2000，19，4： | 4 |
| 20 | Karyotypes of three species of the marine Veneroida molluscs显示文摘The karyotypes of three species of marine Veneroida molluscs, Solen grandis Dunker, Saxidomus purpuratus Sowerby and Mactra chinensis Philippi were studied by using the adult gill tissues. The chromosomes were prepared through injecting phytohemagglutinin (PHA) and colchicine, hypotonic treatment, chopping air-dry, and squashing technology. The results show that the diploid chromosome numbers of the three Veneroida species are the same as 2n=38. The karyotype of Solen grandis is 26m+6sm+2st+4t, NF=70, the karyotype of Saxidomus purpuratus is 32m+2sm+4st/t, NF=72, and the karyotype of Mactra chinensis is 20m+16sm+2st/t, NF=74. Satellite and sex chromosome were not found among the chromosomes of three species. | Sun Zhenxing1, Shao Yanqun1, Guo Shengchao1, Qin Yan1, Yang Aiguo2 1. Department of Biological Science and Biotechnology, Yantai Normal University, Yantai 264025, China. 2. Yellow Sea Fisheries Research Institute, Qingdao 266071, China. | 2003 | Acta Oceanologica Sinica2003，22，4： | 3 |
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