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| 1 | 饲料中水解鱼蛋白对中国对虾非特异免疫的影响显示文摘在中国对虾基础饲料中用水解鱼蛋白替代0%、5%、10%和15%鱼粉蛋白,制成饲料投喂中国对虾30d,实验结束取样测定对虾生长指标并进行血细胞记数,以肝胰脏中的酸性磷酸酶、碱性磷酸酶、溶菌酶、超氧化物歧化酶活性和血清中的酸性磷酸酶、碱性磷酸酶、溶菌酶、超氧化物歧化酶、过氧化物酶、一氧化氮合酶活性为非特异免疫指标,探讨水解鱼蛋白作为免疫增强剂对中国对虾非特异性免疫效应的影响。结果表明,5%水解鱼蛋白替代鱼粉蛋白组对虾肝胰脏中酸性磷酸酶活性为0.54U/gprot,显著高于对照组和其他替代组(P<0.05);血液中总的血细胞数量最多,血清的酸性磷酸酶、碱性磷酸酶和溶菌酶活性均显著高于对照组和其他替代组(P<0.05),并且特定生长率最高。说明水解鱼蛋白作为免疫增强剂对中国对虾的非特异性免疫功能具有明显的增强作用。 | 滕玉清 梁萌青 王正丽 刘宁 王新星 常青 郑珂珂 | 2011 | 渔业科学进展2011，32，5： | 6 |
| 2 | Expression profiles of penaeidin from Fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR显示文摘Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp. | DONGBo LIUFengsong XIANGJianhai LIFuhua GAOHongwei | 2005 | Acta Oceanologica Sinica2005，24，2： | 3 |
| 3 | 感染暴发性流行病病毒的中国对虾形态学研究 3.病虾鳃的显微与超微结构显示文摘在光镜和透射电镜下 ,观察经实验感染暴发性流行病病毒中国对虾的鳃组织 ,发现鳃丝扁平上皮细胞及少量的网状隔膜细胞被感染而引起血隙狭小 ,以及巨噬细胞样无颗粒细胞的趋化作用导致鳃丝扁平上皮细胞增厚而影响其代谢功能 .另外 ,还观察到巨噬细胞样无颗粒细胞有强烈的吞噬作用 .小颗粒细胞也具吞噬作用 ,在染色质凝聚结构等刺激下胞吐小颗粒 .大颗粒细胞也具有胞吐大颗粒功能 .与上述无颗粒细胞相比 ,胃结缔组织血窦内的浆细胞样无颗粒细胞其释放物与病毒结合参与体液免疫 . | 刘昌彬 张红卫 王金星 毕祖刚 吕丽华 赵兴范 张荣芝 | 2002 | 山东大学学报(理学版)2002，37，5： | 1 |
| 4 | 感染暴发性流行病病原的中国对虾形态学研究 2.病虾中肠的显微和亚微结构显示文摘在非生物环境因子对暴发性流行病病原实验感染的中国对虾发病影响的基础上 ,用光镜和电镜观察对虾中肠组织 .观察结果表明 :中肠上皮细胞水样变性 ,少量的上皮细胞被感染 ,肠腔内致病菌继发性感染 ,中肠肌细胞发生凋亡 .大颗粒细胞。 | 刘昌彬 张红卫 刘存仁 毕祖刚 王金星 | 2002 | 山东大学学报(理学版)2002，37，1： | 1 |
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