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| 1 | 氨氮胁迫对奥尼罗非鱼非特异性免疫的影响显示文摘将奥尼罗非鱼(Oreochromis niloticus×O.areus)幼鱼暴露于不同质量浓度(0、2.5 mg·L-1、5 mg·L-1、10 mg·L-1和20 mg·L-1)的氨氮溶液中,于第0、第12、第24、第48和第72小时测定其非特异性免疫相关指标。结果表明,试验幼鱼经氨氮胁迫后各试验组血清溶菌酶活力随着胁迫时间的延长下降显著(P<0.05)。氨氮胁迫明显影响鱼肝脏总抗氧化能力(T-AOC)及相关抗氧化酶活力。与对照组相比,随胁迫时间增加各试验组肝脏TAOC及总超氧化物歧化酶(T-SOD)活力均先下降后上升(P<0.05)。过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活力变化还与胁迫溶液浓度相关。低浓度组(2.5 mg·L-1和5 mg·L-1),CAT活力在胁迫24 h后显著下降(P<0.05);高浓度组(10 mg·L-1和20 mg·L-1),CAT活力却出现先升高后降低的变化。除最高浓度组,各试验组GSH-Px活力表现为诱导效应(P<0.05)。试验条件下氨氮胁迫对罗非鱼非特异性免疫产生明显影响,且随浓度增加及时间延长影响程度加大。 | 韩春艳 郑清梅 陈桂丹 刘丽霞 | 2014 | 南方水产科学2014，10，3： | 35 |
| 2 | Isolation and Characterization of an Algicidal Bacterium Indigenous to Lake Taihu with a Red Pigment able to Lyse Microcystis aeruginosa显示文摘Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FT-IR) spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin (C 20 H 25 N 3 O), which showed strong lytic activity with algal strains M. aeruginosa TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration (IC 50 ) of prodigiosin with the algal strains was 4.8 (±0.4)×10 -2 μg/mL, 8.9 (±1.1)×10-2 μg/mL, and 1.7 (±0.1)×10 -1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment had strong Microcystis-lysing activity probably related to damage of cell membranes. The bacterium LTH-2 and its red pigment are potentially useful for regulating blooms of harmful M. aeruginosa. | YANG Fei WEI Hai Yan LI Xiao Qin LI Yun Hui LI Xiao Bo YIN Li Hong PU Yue Pu | 2013 | Biomedical and Environmental Sciences2013，26，2： | 19 |
| 3 | 氨氮胁迫对刀鲚稚、幼鱼的急性毒性及抗氧化酶的影响显示文摘从抗氧化酶活性的变化揭示氨氮胁迫对刀鲚稚幼鱼毒性效应的机制,为其健康养殖提供科学依据。分别以刀鲚稚鱼(体长2.33 cm±0.25 cm)和幼鱼(体长5.12 cm±0.45 cm)为试验对象,进行96 h氨氮急性毒性试验,得出各自的96 h半致死浓度96hLC50以及安全浓度。然后,分别用刀鲚稚幼鱼40%96 h LC50进行24h的氨氮胁迫试验并取样,然后,将胁迫组剩余的刀鲚放入曝气自来水中进行24 h的恢复试验,胁迫及恢复试验均取全鱼测定其总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)非特异性免疫酶的活性。结果显示:在胁迫24 h后稚幼鱼T-AOC活力显著下降(P<0.05),GSH-Px活力上升但不显著(P>0.05),T-SOD、CAT活力均显著上升(P<0.05);恢复24 h后各种酶的活性都在向正常水平恢复,但是最终都没有恢复到对照组水平。研究获得了氨氮对刀鲚稚幼鱼的急性毒性结果和在氨氮胁迫下抗氧化酶活性的变化规律,氨氮胁迫引起了氧化应激,恢复后抗氧化能力增强,缓解了氧化应激,但并未恢复到对照组水平。 | 杨西伟 徐钢春 高俊 徐跑 | 2021 | 海洋湖沼通报2021，，4： | 7 |
| 4 | Effect of Ammonia-Nitrogen Stress on Non-specific Immunity of Hybrid Tilapia(Oreochromis niloticus× Oreochromis areus)显示文摘The juveniles of hybrid tilapia( Oreochromis niloticus × Oreochromis areus) were exposed to ammonia-nitrogen( N)( 0,2. 5,5,10 and 20 mg / L) for0,12,24,48 and 72 h to evaluate the effect of ammonia-N stress on their non-specific immunity. Results show that the activity of serum lysozyme decreased significantly with extension of stress time( P < 0. 05). The total antioxidant capacity( T-AOC) and activity of antioxidase in liver were significantly affected. The activity of T-AOC and total superoxide dismutase( T-SOD) of fish exposed to ammonia-N were initially decreasing then increasing( P < 0. 05). Activities of catalase( CAT) and glutathione peroxidase( GSH-Px) were correlated with concentrations of ammonia-N. Fish exposed to lower concentrations( 2. 5 mg/L or 5 mg/L)showed decreased CAT activity within 24 h( P < 0. 05),while those exposed to higher concentrations( 10 mg / L or 20 mg / L) initially showed increased then decreased activity of CAT. Except for the highest concentration groups,fish exposed to ammonia-N showed inductive activity of GSH-Px( P < 0. 05). Under the experimental conditions,non-specific immunity of tilapia was affected by ammonia-N stress,and the impact was increased with increased concentration and extension of time. | Han Chunyan Zheng Qingmei Chen Guidan Liu Lixia | 2015 | Animal Husbandry and Feed Science2015，7，4： | 0 |
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