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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Relationship between alcohol consumption and clinical manifestation of patients with fatty liver:a single-center study显示文摘BACKGROUND:Fatty liver is a common chronic liver disease worldwide.It is associated with an increasing morbidity in China in recent years.The aim of this study was to analyze the effect of drinking alcohol on the hemoglobin and biochemical values of patients with fatty liver.METHODS:We investigated the clinical and laboratory data of 669 patients with fatty liver.Of the 669 patients,166 consumed alcohol more than 60 g per week for at least 2 years,and 503 did not have a history of long-term alcohol consumption.We further analyzed the relationship between alcohol consumption and clinical characteristics of these patients.RESULTS:The values of aspartate transaminase (AST),gammaglutamyl transpeptidase (GGT),and hemoglobin in the long-term consumption group were significantly higher than those in the non long-term consumption group (P<0.05).In the patients without long-term alcohol consumption,the values of GGT and hemoglobin in patients with light alcohol consumption were significantly higher than those in non alcohol consumers (P<0.05).CONCLUSION:Alcohol consumption is associated with significantly increased values of AST,GGT,and hemoglobin in patients with fatty liver,suggesting their potential roles in hepatic steatosis. | International Health Care Center (Wang XF) and Department of Gastroenterology (Yue M),First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 5 |
| 3 | Survival factors after resection of small hepatocellular carcinoma显示文摘Early resection of hepatocellular carcinoma is a key measure to prolong the survival of patients. This study was designed to summarize our experience in surgical resection of small hepatocellular carcinoma (HCC), and to analyze the factors influencing the postoperative survival of patients. METHODS:The clinicopathologic data of 105 patients with small HCC after resection from 1986 through 2003 were analyzed ; the patients had been followed up for more than half a year (median 33 months). Nine clinicopathologic factors, preoperative α-fetoprotein (AFP) level, liver cirrhosis, Child-Pugh score, tumor size (>2cm vs.≤2 cm) and number (single vs. multiple), capsule formation, portal vein tumor thrombi (PVTT), Edmondson tumor grade and surgical method, were analyzed by the log-rank test and the Cox proportional harzards model analysis. RESULTS:The cumulative 1-,3- and 5-year survival rates after the operation were 86.5%, 70.3% and 55.2%, respectively, and the 1-, 3- and 5-year disease-free survival rates were 78%, 58. 9% and 45. 6%, respectively. One patient died from esophagogastric varices hemorrhage in 2 weeks after reoperation. Thirty-six patients had intrahepatic recurrence or metastasis postoperatively and 34 patients died. The Kaplan-Meier method and the Cox proportional harzards model analysis indicated that poor Child-Pugh score, tumor more than 2 cm in diameter, PVTT and multiple lesions (including satellitic lesions) were adverse factors affecting postoperative survival. The Cox proportional harzards model analysis indicated that tumor size, PVTT and multiple lesions were the factors influencing postoperative disease-free survival. CONCLUSIONS:Limited hepatectomy with a margin more than 1 cm is an appropriate surgical approach. Adverse preoperative Child-Pugh score and postoperative intrahepatic recurrences are the main factors leading to the death of patients with small HCC. | Departments of Surgical Oncology (Wu FS, Zhao WH, Ma ZM, Teng LS and Wang M) and General Surgery ( Liang TB and Zheng SS) , First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 5 |
| 4 | Definiing regions of the Y - chromosome responsible for male infertility and identification of a fourth AZF region (AZFd) by Y - chromosome microdeletion detection显示文摘 | Kent - First M Muallem A SchultzJ | 1999 | Mol Reprod Dev1999，53，： | 1 |
| 5 | Mortality in Patients with Hemophilia: Changes in a Dutch Population form 1986 to1992 and 1973 to 1986显示文摘 | Mattanla M First R Rosendaal M D | 1995 | Ann Intern Med1995，123，11： | 1 |
| 6 | Defining regionsof the Yechromosome responsible formale infertility and identification of a fourthAZFregion(AZFd) by Y chromo some micro deletion detection显示文摘 | Kent First M Muallem A Shuhz J | 1999 | MolReprod Dev1999，53，1： | 1 |
| 7 | Defining regions of the Y chromosome responsible for male infertility and identification of afourth AZF region (AZFd) by Y chromosome microdeletion detection 显示文摘 | Kent - First M Muallem A Shultz J | 1999 | Mol Rep rod Dev1999，53，1： | 1 |
| 8 | The Effects of fluoxetinetreatment in a chronic mild stress ratmodel on depression-re-lated behavior,brain neurotrophins and ERK expression显示文摘 | First M Gil-Ad I Taler M | 2011 | J Mol Neurosci2011，45，2： | 1 |
| 9 | The effects of reboxetine treatment on depression -like behavior, brain neurotrophins, and ERK expression in rats exposed to chronic mild stress 显示文摘 | First M Gil-Ad I Taler M | 2012 | J Mol Neurosci2012，50，1： | 1 |
| 10 | Conversion From Cyclosporine to Tacrolimus in Patients at Risk for Chronic Renal Allograft Failure: 60-Month Results of the CRAF Study显示文摘 | Fuad S. Shihab Thomas H. Waid David J. Conti Harold Yang Michael J. Holman Laura C. Mulloy Alice K. Henning John Holman M Roy First | 2008 | Transplantation2008，，9： | 1 |
| 11 | Acute interstitial nephritis显示文摘 | Jao W First M R | 1975 | Am J Med1975，59，5： | 1 |
| 12 | Defining regions of the Y-chronmsome responsible for male infertility and identification of a fourth AZF region (AZFd) by Y-chromosome microdeletion detection显示文摘 | KENT FIRST M MUALLEM A SHULTZ J | 1999 | Mol Reprod Dev1999，53，： | 1 |
| 13 | The effects of fluoxetine treatment in a chronic mild stress rat model on depression-related behavior,brain neurotrophins and ERK expression显示文摘 | FIRST M GIL AD L TALER M | 2011 | J Mol Neurosci2011，45，2： | 1 |
| 14 | The application of ultraviolet germicidal irradiation to control teansmission of airbome disease: bioterrorism countermeasure 显示文摘 | Brickner P W Vincent R L First M | 2003 | Public Health Rep2003，118，2： | 1 |
| 15 | In vitro inhibition of oocyte nuclear maturation in the bovine显示文摘 | Sirard M A First N L | 1988 | Biol-Reprod1988，39，2： | 1 |
| 16 | Bovine ocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species显示文摘 | Dominko T Massam M Haley B Beyhan Z Melili E Mckusick B First N L | | 0，，： | 1 |
| 17 | Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocytes maturation 显示文摘 | Fulka J J First N L Moor R M | 1998 | Human Reproduction1998，4，： | 1 |
| 18 | Culture of porcine embryos from the one and two-cell stage to the blastocyst stage in sheep oviducts 显示文摘 | PRATHER R S SIMS M M FIRST N L | 1991 | Thenogenology1991，35，： | 1 |
| 19 | Growth and grazing rates of bacteria groups with different apparent DNA content in the Gulf of Mexico显示文摘 | Jochem F J Lavrentyev P J First M R | 2004 | Marine Biology2004，145，6： | 1 |
| 20 | Developmental and molecular correlates of bovine preimplantation embryos显示文摘 | Sagirkaya H Misirlioglu M Kaya A First N L Parrish J J Memili E | | 0，，5： | 1 |
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