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您的检索式：作者名="FAN Meining"
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| 1 | 抑制Pin1通过NF-κB途经抑制大肠癌HCT116细胞的端粒酶(英文)显示文摘Objective: The aim of our study was to investigate the effect of Pin1 on the telomerase activity in human colorectal carcinoma HCT116 cells. Methods: Firstly, we transfected plasmid pGenesil-1-Pin1 (p-shRNA) using liposome (Lipofectamine 2000) into colorectal cancer HCT-116 cells to down-regulate the expression of Pin1. To detect the apoptotic rate of HCT116 cells was by cytometry (FCM). The expression of Pin1 and hTERT at RNA levels in human colorectal cancer HCT116 cells were determined by RT-PCR. To evaluate the activity of telomerase was by TRAP-silver staining. The subcellular localization and accumulative level of p-NF-κB/p65 protein at the nuclear was detected by Immunofluorescence and Western blotting. The DNA-binding activity of NF-κB/p65 was detected by electrophoretic mobility shift assay (EMSA). Results: Using liposome into colorectal cancer HCT-116 cells, and down-regulate the expression of Pin1 (0.392 ± 0.072-fold; P = 0.001), and the apoptotic rate was increased (11.40% ± 1.54%; P < 0.05). Compared with transfected p-CON cell group, in transfected p-shRNA cell group, the transcription of hTERT was lower (0.171 ± 0.060-fold; P = 0.001) by quantitative real-time RT-PCR, and the results of TRAP-silver staining analysis suggested that the telomerase activity was significantly declined (0.384 ± 0.015-fold; P < 0.05). Furthermore, it was demonstrated by Immunofluorescence that p-NF-κB/p65 had a nuclear localization, and the level of p-NF-κB/p65 protein at the nuclear was reduced with silencing the expression of Pin1 by Western blotting. Using EMSA, it was suggested that NF-κB/p65 was able to bind to hTERT promoter, and the direct interaction was declined with silencing the expression of Pin1. Conclusion: Taken together, silencing Pin1 may suppress activity of telomerase and the expression of hTERT by inhibiting NF-κB/p65 activity and reducing the combination of NF-κB/p65 and hTERT gene promoter. | Jianwen Sun Lijun Fan Meining Li Yuehong Zhang Niuliang Cheng | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，4： | 0 |
| 2 | Calculation of seismic transmission speed of HTI medium显示文摘During the seismic wave propagation process,as for the anisotropic lower medium,the speed is a function of the propagating direction.This article focuses on solving the problem how to get the transmittance angle and speed,knowing the upper seismic wave propagation velocity and the angle of incidence conditions.The main theories used Snell law,Christoffel equation and speed characteristics.Taking the HTI medium as an example,the authors give the detailed solving process and draw the space velocity characteristic curve. | WANG Hongwei FAN Meining | 2013 | Global Geology2013，16，4： | 0 |
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