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| 1 | Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development显示文摘Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its root.This study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast cancer.Methods: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo assay.Results: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated animals.Conclusion: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy. | Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia Adolfo Erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo J.S.Pereira André Hinsberger Ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto | 2019 | Journal of Integrative Medicine2019，17，2： | 2 |
| 2 | Determination of chloride by multisyringe flow injection analysis and sequential injection analysis with potentiometric detection 显示文摘 | Andrade - Eiroa A Erustes JA Forteza R | 2002 | Analytica Chimica Acta2002，467，1： | 1 |
| 3 | Determination of chloride by multisyringe flow injection analysis and sequential injection analysis with potentiometric detection显示文摘 | Andrade-Eiroa A Erustes J A Forteza R | 2002 | Anal Chim Acta2002，467，12： | 1 |
| 4 | Plasmafitrinogen2anindependentcardiovascularriskfactor显示文摘 | ErustE | 1990 | J lntern Med1990，227，6： | 1 |
| 5 | Mineral parageneses in Franciscan metamorphic rocks,Panoche Pass,California显示文摘 | Erust W G | 1965 | Geological Society of America Bulletin1965，76，： | 1 |
| 6 | Plasma fibrinogen –an independent cardiovascular risk factor显示文摘 | Erust E | 1990 | Inter Med1990，277，： | 1 |
| 7 | A Potential Alternative for Precious Metal Recovery from E-waste: Iodine Leaching显示文摘 | Merve Sahin Ata Akcil Ceren Erust Shinar Altynbek Chandra Sekhar Gahan Aysenur Tuncuk | 2015 | Separation Science and Technology2015，，16： | 1 |
| 8 | Fibrincugess as a cardiovascular risk factor:A metaanalyais and review of the literature显示文摘 | Erust E Resch K L | 1993 | Am Intern Med1993，118，8： | 1 |
| 9 | Alasma fibfinogen an independent cardiovascular risk factor显示文摘 | Erust E | 1990 | Jintemmed1990，227，6： | 1 |
| 10 | Fast sequent injection determination of benzo pyrene using variable angle fluorescence with on - line solid - phase extraction 显示文摘 | Erustes JA An&Me - Erioa A cladrca A | 2001 | Analyst2001，126，4： | 1 |
| 11 | Fast sequential injection determination of benzo pyrene using variable angle fluo- rescence with on - line solid - phase extraction显示文摘 | Erustes JA Andrade - Erioa A Cladrca A | 2001 | Analyst2001，126，4： | 1 |
| 12 | Efficient tumor regression by a single and low dose treatment with a novel and enhanced formula- tion of thermosensitive liposomal doxorubicin显示文摘 | Tagami T Erusting M J Li SD | 2011 | J Control Re- lease2011，152，2： | 1 |
| 13 | Optimizing the energy efficiency of conventional multicylinder dryers in the paper industry 显示文摘 | Laurijssen Jobien De Gram Frans J Worrell Erust | 2010 | Energyl2010，35，9： | 1 |
| 14 | Preconditioning with Tanscranial Direct Current Stimulation Sensitizes the Motor Cortex to Rapid-Rate Transeranial Magnetic Stimulation and Controls the Direction of After-Effects显示文摘 | Lang N Siebner HR Erust D | 2004 | Biol Psychiatry2004，56，： | 1 |
| 15 | Fibrincugess as a cardiovascular risk factor:A metaanalysis and review of the literature显示文摘 | ERUST E RESCH KL | 1993 | Ami intern med1993，118，： | 1 |
| 16 | Changes in blood volume during dialysis are dependent upon the rate and amount of uptrafiltrite显示文摘 | Mann H Erust E Gladziwa U | 1989 | Trans Am Soc Artif Organs1989，35，： | 1 |
| 17 | Fibrincugess as a cardiovascular risk factor: A meta-analysis and review of the literature显示文摘 | Erust E Resch KL | 1993 | Am Intern Med1993，118，8： | 1 |
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