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| 1 | 超临界流体染色技术的过去、现在和将来(一)显示文摘介绍了超临界CO2 染色技术在世界范围的发展过程及其优于水染色的特点。阐述了超临界CO2 对纤维性能的影响 ,诸如润滑效果、CO2 吸附、结晶、收缩和热机械性能 ,超临界CO2 对纤维的玻璃化温度和有效温度以及对纤维损伤的影响。另一重要问题是在超临界CO2 中纤维与染料之间的平衡分布和影响染料溶解性的参数。文中给出了涤纶纤维的染色步骤、设备和最佳工艺参数 ,然而天然纤维的染色仍是一个问题。通过浸渍溶胀剂和交联剂 ,或在染色前对纤维改性、添加极性共溶剂 ,可以提高分散染料的染色性 ,所有这些方法增加了工艺步骤和设备清洗 ,若使用有机溶剂则将大大提高机器成本 ,采用活性分散染料可以避免这种情况。含乙烯砜基团的染料主要适宜于羊毛和聚酰胺纤维的超临界CO2染色 ,而对于纤维素纤维迄今尚未开发可行的解决方法。预计在不久的将来建造工业化规模的染色设备 ,努力实现超临界CO2 染色技术的经济效益。 | Elke Bach Ernst Cleve Eckhard Schollmeyer | 2003 | 印染2003，29，3： | 32 |
| 2 | 纳米技术在锂二次电池中的应用显示文摘对近几年来纳米技术在锂二次电池中的应用进行了综述。所用纳米技术包括溶胶 凝胶法、模板法、碳棒电弧法和机械研磨法等,制备的锂二次电池纳米材料包括正极、负极和其它材料。纳米正极材料包括氧化钴锂、氧化镍锂、氧化锰锂和钒的氧化物等,纳米负极材料包括碳材料、富勒烯、碳纳米管、氧化锡和金属负极材料等,其它纳米材料包括聚合物电解质和导电剂。这些纳米材料的电化学性能较一般方法制备的材料有明显的提高,但是目前在锂二次电池领域对纳米技术的理论研究还有待于深入。随着纳米技术的不断发展,微型锂二次电池的诞生为期不远。 | 吴宇平 Rahm Elke Holze Rudolf | 2002 | 电池2002，32，6： | 12 |
| 3 | Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana显示文摘联系压力的蛋白质 SAP12 属于联系压力的蛋白质(树液) 与在 Arabidopsis thaliana 的 14 个成员一起的家庭。SAP12 包含二根 AN1 锌手指并且作为经历主要氧化还原作用依赖者 conformational 变化的蛋白质在斜 2D 氧化还原作用 SDSPAGE 被识别。它的抄本强烈以类似于 SAP10 的一种时间依赖者方式在冷、咸的应力下面被导致,与在 6 以后的高水平 ? 在 24 和 48 以后的 h 和减少的层次 ? h。抄本规定在 24 和 48 点压力标记 peroxiredoxin PrxIID 类似于那些 ? h。Recombinant SAP12 蛋白质在在非减少的 SDS polyacrylamide 胶化电气泳动由改变的 electrophoretic 活动性设想了的第四级的结构显示出氧化还原作用依赖者变化。氧化 oligomer 被高 dithiothreitol 集中减少,并且另外由 E。有低 dithiothreitol (DTT ) 的 coli thioredoxin TrxA 加 NADPH 依赖的 thioredoxin reductase 的集中或 NADPH。从西方的污点, SAP12 蛋白质数量被估计在 0.5 的范围 ? ng ? g ? 1 叶蛋白质。SAP12 蛋白质在盐和冷应力下面减少了。这些数据建议一个氧化还原作用在特别地在冷、咸的应力下面的植物房间的 SAP12 的连接状态的功能。 | Elke Stroeher Xin-Jia Wang Nils Roloff Peter Klein Arne Husemann Karl-Josef Dietz | 2009 | Molecular Plant2009，2，2： | 11 |
| 4 | Effect of dietary supplementation of xylanase on apparent ileal digestibility of nutrients,viscosity of digesta,and intestinal morphology of growing pigs fed corn and soybean meal based diet显示文摘This study was to determine apparent ileal digestibility of acid detergent fiber(ADF), neutral detergent fiber(NDF), dry matter(DM), energy, organic matter(OM), crude ash, digesta viscosity, and gut morphology in nursery pigs fed diets containing xylanase(Lohmann Animal Nutrition GmbH, Cuxhaven, Germany). The diet(61% corn, 35% soybean meal, 1% poultry fat, and 3% minerals and vitamins) was mixed with 3 levels of xylanase(0,700, and 1400 LXU/kg). Thirty-six barrows(17.6 ± 3.3 kg) received one of 3 treatment diets based on a randomized complete block design with the initial body weight(BW) as a block. Pigs were individually housed and received experimental diets twice daily(0700 and 1700 h) at a fixed amount based on BW of pigs(0.09 x BW^(0.75) kg). Pigs were fed diets for 10 d, and chromium oxide(0.3%) was added to the diets from d 6 as an indigestible external marker. Pigs were euthanized at the end of d 10 for the collection of digesta and tissues. Jejunal digesta were centrifuged to measure viscosity using a viscometer(Brookfield Engineering Laboratories, Stoughton, MA). Diets and freeze-dried ileal digesta were used to measure ADF, NDF, and chromium to calculate apparent ileal digestibility of ADFand NDF. Villus height and crypt depth of jejunum were measured using a microscope(Fisher Scientific, Hampton, NH). Data were analyzed using polynomial contrasts in the MIXED procedure of SAS version 9.3(SAS Inc., Cary, NC, USA). Morphological measurements and ileal ADF digestibility were not affected by increasing xylanase. However, increasing xylanase supplementation from 0 to 1400 LXU/kg enhanced ileal digestibility of NDF(P < 0.042, linear) from 27.9 to 40.3%,DM(P < 0.006, linear) from 55.4 to 64.6%, OM(P < 0.006, linear) from 59.2 to 67.7%, and energy(P < 0.003,linear) from 58.8 to 68.0%. Viscosity of jejunal digesta decreased(P < 0.023) in a non-linear manner from 2.9 to 2.5 centipoises(cP). In conclusion, the usage of xylanase in corn and soybean meal based pig diets linearly enhanced digestibility of nutrients and affected viscosity of digesta in a non-linear manner. | Adsos Adami Passos Inkyung Park Peter Ferket Elke von Heimendahl Sung Woo Kim | 2015 | Animal Nutrition2015，，1： | 10 |
| 5 | 肠的障碍: 分子的小径和修饰词显示文摘 The gastrointestinal tract is frequently challenged by pathogens/antigens contained in food and water and the intestinal epithelium must be capable of rapid regeneration in the event of tissue damage. Disruption of the intestinal barrier leads to a number of immune-mediated diseases, including inflammatory bowel disease, food allergy, and celiac disease. The intestinal mucosa is composed of different types of epithelial cells in specific barrier functions. Epithelial cells control surfaceassociated bacterial populations without disrupting the intestinal microflora that is crucial for host health. They are also capable of modulating mucosal immune system, and are thus essential in maintaining homeostasis in the gut. Thus, the regulation of intestinal epithelial homeostasis is crucial for the maintenance of the structure of the mucosa and the defensive barrier functions. Recent studies have demonstrated that multiple molecular pathways are involved in the regulation of intestinal epithelial cell polarity. These include the Wnt, Notch, Hippo, transforming growth factor-β(TGF-β)/bone morphogenetic protein(BMP) and Hedgehog pathways, most of which were identified in lower organisms where they play important roles during embryogenesis. These pathways are also used in adult organisms to regulate multiple self-renewing organs. Understanding the interactions between these molecular mechanisms and intestinal barrier function will therefore provide important insight into the pathogenesis of intestinal-based immune-mediated diseases. | Min Kyung Jeon Christina Klaus Elke Kaemmerer Nikolaus Gassler | 2013 | World Journal of Gastrointestinal Pathophysiology2013，4，4： | 10 |
| 6 | Cellular Auxin Homeostasis: Gatekeeping Is Housekeeping显示文摘植物激素植物生长素为植物开发是必要的并且将近贡献植物生命周期的每个方面。植物生长素的时间空间的分发取决于在植物生长素新陈代谢和 cell-to-cell 植物生长素运输之间的复杂相互影响。植物生长素新陈代谢和运输为植物开发两个都是关键的;然而,这些过程怎么是综合的在纸巾或机关以内保证定义细胞的植物生长素层次或甚至坡度,大部分尚待分晓。在这评论,我们在植物生长素生物学的很多样的话题提供一瞥,例如植物生长素的生合成,变化形式,氧化,和运输。这宽广,却当然表面,概述强调植物生长素新陈代谢和运输的相互的重要性。而且,它允许指出植物生长素新陈代谢和运输怎么变得综合联合调整细胞的植物生长素动态平衡。尽管这些过程到目前为止仅仅独立被学习了,我们假设 phytohormonal 串音集成并且协调植物生长素新陈代谢和运输。除发信号的全球荷尔蒙的综合力量以外,我们另外为植物生长素回答作为看门人就植物生长素运输部件而言介绍假想概念。 | Michel Ruiz Rosquete | 2012 | Molecular Plant2012，5，4： | 9 |
| 7 | Enhanced migration of tissue inhibitor of metalloproteinase overexpressing hepatoma cells is attributed to gelatinases: Relevance to intracellular signaling pathways显示文摘AIM: To study the effect of gelatinases (especially MMP-9)on migration of tissue inhibitor of metalloproteinase (TIMP-1) overexpressing hepatoma cells.METHODS: Wild type HepG2 cells, cells stably transfected with TIMP-1 and TIMP-1 antagonist (MMP-9-H401A, a catalytically inactive matrix metalloproteinase (MMP) which still binds and neutralizes TIMP-1) were incubated in Boyden chambers either with or without Galardin (a synthetic inhibitor of MMP-1, -2, -3, -8, -9) or a specific inhibitor of gelatinases.RESULTS: Compared to wild type HepG2 cells, the cells overexpressing TIMP-1 showed 115% migration (P<0.05)and the cells overexpressing MMP-9-H401A showed 62% migration (P<0.01). Galardin reduced cell migration dose dependently in all cases. The gelatinase inhibitor reduced migration in TIMP-1 overexpressing cells predominantly.Furthermore, we examined intracellular signal transduction pathways of TIMP-1-dependent HepG2 cells. TIMP-1deactivates cell signaling pathways of MMP-2 and MMP-9involving p38 mitogen-activated protein kinase. Specific blockade of the ERK pathway suppresses gelatinase expression either in the presence or absence of TIMP-1.CONCLUSION: Overexpressing functional TIMP-1-enhanced migration of HepG2-TIMP-1 cells depends on enhanced MMP-activity, especially MMP-9. | Elke Roeb Anja-Katrin Bosserhoff Sabine Hamacher Bettina Jansen Judith Dahmen Sandra Wagner Siegfried Matern | 2005 | World Journal of Gastroenterology2005，11，8： | 7 |
| 8 | Collagen/hyaluronan based hydrogels releasing sulfated hyaluronan improve dermal wound healing in diabetic mice via reducing inflammatory macrophage activity显示文摘Sustained inflammation associated with dysregulated macrophage activation prevents tissue formation and healing of chronic wounds.Control of inflammation and immune cell functions thus represents a promising approach in the development of advanced therapeutic strategies.Here we describe immunomodulatory hyaluronan/collagen(HA-AC/coll)-based hydrogels containing high-sulfated hyaluronan(sHA)as immunoregulatory component for the modulation of inflammatory macrophage activities in disturbed wound healing.Solute sHA downregulates inflammatory activities of bone marrow-derived and tissue-resident macrophages in vitro.This further affects macrophage-mediated pro-inflammatory activation of skin cells as shown in skin ex-vivo cultures.In a mouse model of acute skin inflammation,intradermal injection of sHA downregulates the inflammatory processes in the skin.This is associated with the promotion of an anti-inflammatory gene signature in skin macrophages indicating a shift of their activation profile.For in vivo translation,we designed HA-AC/coll hydrogels allowing delivery of sHA into wounds over a period of at least one week.Their immunoregulatory capacity was analyzed in a translational experimental approach in skin wounds of diabetic db/db mice,an established model for disturbed wound healing.The sHA-releasing hydrogels improved defective tissue repair with reduced inflammation,augmented pro-regenerative macrophage activation,increased vascularization,and accelerated new tissue formation and wound closure. | Sophia Hauck Paula Zager Norbert Halfter Elke Wandel Marta Torregrossa Ainur Kakpenova Sandra Rother Michelle Ordieres Susann Räthel Albrecht Berg Stephanie Möller Matthias Schnabelrauch Jan C.Simon Vera Hintze Sandra Franz | 2021 | Bioactive Materials2021，6，12： | 7 |
| 9 | Multiple Redox and Non-Redox Interactions Define 2-Cys Peroxiredoxin as a Regulatory Hub in the Chloroplast显示文摘在植物,高度丰富的 2 半胱氨酸 peroxiredoxin (2-CysPrx ) 与叶绿体被联系并且在保护光合作用包含了。这个工作在叶绿体探讨 2-CysPrx 的多重相互作用,它取决于它的氧化还原作用状态。抄本合作规定分析显示出一个强壮的连接到 peptidyl-prolyl-cis/trans isomerase Cyclophilin 20-3 (Cyp20-3 ) 和光合的仪器的另外的部件。在荧光回声精力转移的原物和 quantification 的合作表示(烦恼) 在 vivo 的效率与 Cyp20-3 以及 NADPH 依赖的 thioredoxin reductase C (NTRC ) 证实了 2-CysPrx 的蛋白质相互作用,当 thioredoxin x (Trxx ) 没形成能启用的建筑群时,烦恼。同样,变化在在 vitro 并且在 vivo 荧光灯地标记的 2-CysPrx 烦恼 2-CysPrx 的证明氧化还原作用依赖者动力学。Cyp20-3 到的增加一在里面有 2-CysPrx 的 vitro peroxidase 试金没穿上重要效果过氧化物减小。面对 NTRC,另外, Cyp20-3 的增加进一步没提高过氧化物减小。另外, 2-CysPrx 作为女伴工作了并且在热处理期间禁止了柠檬酸盐 synthase 的聚集。这项活动被 Cyp20-3 部分禁止。作为 decameric 2-CysPrx 的一个新相互作用搭挡, photosystem II 能在宪法以后在叶绿体分别以后并且在下拉试金被识别。在摘要,数据作为 thiol peroxidase 在它的角色以外与多样的函数在叶绿体作为氧化还原作用传感器,女伴,和管理者显示植物 2-CysPrx 的一个动态函数。 | Meenakumari Muthuramalingam Thorsten Seidel Miriam Laxa Susana M. Nunes de Miranda Florian Gartner Elke Stroher Andrea Kandlbinder Karl-Josef Dietz | 2009 | Molecular Plant2009，2，6： | 7 |
| 10 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 11 | Toll-Like Receptor Expression in the Peripheral Nerve显示文摘Toll样受体(TLRs)是由进化上相对保守的蛋白家族构成的一组识别受体,是固有免疫系统的第一道防线。受到微生物配体刺激后,TLRs通过激活各种信号通路引发机体一系列防御反应。TLRs还能被内源性危险信号激活,因此推断神经退行性变也可能通过TLRs激活免疫反应。近来研究发现中枢神经系统表达各种类型的TLRs,但周围神经系统中TLRs的表达形式尚无定论。本研究结果显示,施万细胞上表达大量TLRs,主要为TLR3和TLR4。感觉神经元和运动神经元上几乎没有TLRs表达。对NF-κB信号通路的检测显示,施万细胞上所有的TLRs都是有功能的,可被细菌脂蛋白(TLR1/TLR2配体)激活,产生强烈反应。静息状态下,坐骨神经上TLRs的表达形式与施万细胞相似,主要为TLR3、TLR4和TLR7,可能在免疫监督中扮演重要角色。通过显微外科方式对坐骨神经造成急性神经退行性变后,诱导TLR1大量表达,其它TLRs的表达水平无变化。综上所述,本研究结果显示,施万细胞可能在周围神经中扮演类似中枢神经系统小胶质细胞的角色。急性神经退行性变诱导TLR表达变化,说明在分布于周围神经的TLRs在静息状态和激活状态下具有不同功能。 | SOFIE GOETHALS ELKE YDENS VINCENT TIMMERMAN SOPHIE JANSSENS | 2010 | 神经损伤与功能重建2010，5，6： | 6 |
| 12 | Transcriptome Analysis of High-Temperature Stress in Developing Barley Caryopses: Early Stress Responses and Effects on Storage Compound Biosynthesis显示文摘高温度的应力象任何不能生活的压力一样,损害植物的生理学和发展,包括种子设定并且成熟的阶段。我们使用了调查开发大麦(Hordeum vulgare ) 的反应的 GeneChip microarray 播种的 Affymetrix 22K Barley1,称为的 caryopses 在 0.5 以后, 3,和 6 ? 热压力暴露的 h;958 导致了, 1122 镇压了空间的展出的基因和提供详细卓见进 caryopse 的早热的时间的表示模式强调回答。与存储混合物生合成和细胞生长有关的基因的下面规定为 caryopsi 的发展的一个快速的缺陷提供证据。糖和氨基酸的增加的层次为兼容溶质的生产和为存储混合物生合成的底层的导致反馈的累积是指示的。当热的主要地点强调回答,元数据分析识别了胚胎和内乳,显示颖果的中央发展函数上的短期的热应力的强壮的影响。有在 Arabidopsis 射击的热压力回答和在大麦 caryopses 的干旱压力回答的比较识别了保存并且大概热特定、颖果特定的压力应答的基因。总结,我们的数据提供一个重要基础让基因功能的进一步的调查以便为谷物庄稼作为一个模型在大麦帮助改进的热忍耐和产量的减少的损失。 | Elke Mangelsen Joachim Kilian Klaus Harter Christer Jansson Dierk Wanke Eva Sundberg | 2011 | Molecular Plant2011，4，1： | 6 |
| 13 | 性别和年龄对中国人冠状动脉旁路移植术后住院病死率的影响显示文摘目的确定中国人冠状动脉旁路移植术后住院病死率是否存在性别差异以及该差异是否与年龄相关.方法回顾性分析1997年1月1日至2001年12月31日阜外心血管病医院外科全部接受冠状动脉旁路移植术的患者共2682例(男性2316例,女性366例).按照性别和年龄分组调查它们之间的围手术期并发症及手术后住院病死率的差异.结果女性术前合并主要疾病的比例比男性高,术后并发症多,但她们术前的左心室射血分数较男性好,且冠状动脉病变数量少.然而,女性患者的术后住院病死率是男性的3倍(女性3.01%,男性1.12%,P=0.001),病死率的性别差异在低龄组更有统计学意义(女性2.6%,男性0.5%,P=0.001,ORs:4.844,95%可信区间:1.549~15.142),在老年患者中则无统计学意义(女性3.7%,男性2.4%,P=0.383).结论中国女性冠状动脉旁路移植术的住院病死率高于男性,特别是低龄女性.低龄组女性是住院病死率的独立危险因素.死亡率的性别差异随年龄增加而减少.需要进一步的研究来阐明病死率的性别差异在低龄组更显著的原因. | 宫路佳 Elke Lehmkuhl 胡盛寿 Friederike Kendel Stein Kandle Yong Du 章晏 杜茗 吴清玉 许建屏 孙立忠 朱晓东 Vera Regitz-Zagrosek | 2006 | 中华心血管病杂志2006，34，5： | 5 |
| 14 | P-glycoprotein multidrug transporter in inflammatory bowel diseases: More questions than answers显示文摘The gastrointestinal barrier is constantly exposed to numerous environmental substrates that are foreign and potentially harmful. These xenobiotics can cause shifts in the intestinal microbiota composition, affect mucosal immune responses, disturb tissue integrity and impair regeneration. The multidrug transporter ABCB1/MDR1 p-glycoprotein (p-gp) plays a key role at the front line of host defence by efficiently protecting the gastrointestinal barrier from xenobiotic accumulation. This Editorial discusses how altered expression and function of ABCB1/MDR1 p-gp may contribute to the development and persistence of chronic intestinal inflammation in inflammatory bowel diseases (IBD). Recent evidence implies multiple interactions between intestinal microbiota, innate immunity and xenobiotic metabolism via p-gp. While decreased efflux activity may promote disease susceptibility and drug toxicity, increased efflux activity may confer resistance to therapeutic drugs in IBD. Mice deficient in MDR1 A develop spontaneously chronic colitis, providing a highly valuable murine IBD model for the study of intestinal epithelial barrier function, immunoregulation, infectious co-triggers and novel therapeutic approaches. Possible associations of human ABCB1 gene polymorphisms with IBD susceptibility have been evaluated, but results are inconsistent. Future studies must focus on further elucidation of the pathophysiological relevance and immunological functions of p-gp and how its ambiguous effects could be therapeutically targeted in IBD. | Elke Cario | 2017 | World Journal of Gastroenterology2017，23，9： | 5 |
| 15 | Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity显示文摘AIM:To investigate the role of acyl-CoA synthetase 5(ACSL5)activity in Wnt signaling in intestinal surface epithelia.METHODS:Several cell lines were used to investigate the ACSL5-dependent expression and synthesis of Wnt2B,a mitochondrially expressed protein of the Wnt signaling family.Wnt activity was functionally assessed with a luciferase reporter assay.ACSL5-related biochemical Wnt2B modifications were investigatedwith a modified acyl-exchange assay.The findings from the cell culture models were verified using an Apcmin/+mouse model as well as normal and neoplastic diseased human intestinal tissues.RESULTS:In the presence of ACSL5,Wnt2B was unable to translocate into the nucleus and was enriched in mitochondria,which was paralleled by a significant decrease in Wnt activity.ACSL5-dependent S-palmitoylation of Wnt2B was identified as a molecular reason for mitochondrial Wnt2B accumulation.In cell culture systems,a strong relation of ACSL5 expression,Wnt2B palmitoylation,and degree of malignancy were found.Using normal mucosa,the association of ACSL5 and Wnt2B was seen,but in intestinal neoplasias the mechanism was only rudimentarily observed.CONCLUSION:ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity.The molecular pathway is probably relevant for intestinal homeostasis,overwhelmed by other pathways in carcinogenesis. | Christina Klaus Ursula Schneider Christian Hedberg Anke K Schütz Jürgen Bernhagen Herbert Waldmann Nikolaus Gassler Elke Kaemmerer | 2014 | World Journal of Gastroenterology2014，20，40： | 4 |
| 16 | 固相微萃取/气相色谱-质谱联用技术在有机磷酸三酯阻燃剂土壤吸附分析中的应用显示文摘以3种常用且极性相差较大的有机磷酸三酯阻燃剂(OPFRs)作为研究对象,采用固相微萃取/气相色谱-质谱(SPME/GC-MS)联用技术建立了一种适用于研究OPFRs土壤吸附的分析方法:优化固相微萃取的萃取条件——取样体积为7mL,萃取时间为30min,解吸温度和时间分别为270℃和5min;分析测定过程中导致空白值升高的干扰因素;针对不同类型的土壤分别绘制标准曲线。利用已建立的SPME/GC-MS法对3种OPFRs在不同类型土壤中的吸附程度进行了初步评估,结果表明:3种OPFRs在试验土壤中的吸附率为27%~69%,吸附程度随着OPFRs极性的减弱以及土壤有机质含量的增加而增加。该方法具有操作简单,分析速度快,准确度、灵敏度和精密度均能满足定量测试要求,空白干扰小以及易于普及等特点。 | 郑春莉 冯珊珊 杜苗苗 齐月 Elke FRIES | 2015 | 西安交通大学学报2015，49，11： | 4 |
| 17 | A broker-based semantic agent for discovering Semantic Web services through process similarity matching and equivalence considering quality of service显示文摘The lack of semantic descriptions for 'web service properties' makes it difficult to find suitable web services.Current solutions are mostly based on broker/mediator agent systems.However,these techniques are syntactical,rather than semantics oriented.This article presents a semantic matching approach for discovering Semantic Web services through a broker-based semantic agent(BSA).The BSA includes knowledge-bases and several processing steps.The BSA's knowledge-bases are concept,task,and process ontologies built to describe both functional and non-functional parameters of services.The BSA executes semantic-based matching algorithms to discover similar services through the semantic matching step,process equivalence task,and matching of quality of service parameters.Relevant services are ranked by client preferences utilizing the semantic descriptions of available services.Other matchmaker studies are reviewed and compared with the BSA.Performance of the BSA algorithm is compared with SAM using published data and an experimental setup.The results indicate that our approach is better and more effective in some respects. | ELK Duygu EL Atilla | 2013 | Science China(Information Sciences)2013，56，1： | 4 |
| 18 | Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C显示文摘AIM:To investigate whether human acyl-CoA synthetase 5(ACSL5) is sensitive to the ACSL inhibitor triacsin C.METHODS:The ACSL isoforms ACSL1 and ACSL5 from rat as well as human ACSL5 were cloned and recombinantly expressed as 6xHis-tagged enzymes.Ni 2+-affinity purified recombinant enzymes were assayed at pH 7.5 or pH 9.5 in the presence or absence of triacsin C.In addition,ACSL5 transfected CaCo2 cells and intestinal human mucosa were monitored.ACSL5 expression in cellular systems was verified using Western blot and immunofluorescence.The ACSL assay mix included TrisHCl(pH 7.4),ATP,CoA,EDTA,DTT,MgCl 2,[9,103 H] palmitic acid,and triton X-100.The 200 μL reaction was initiated with the addition of solubilized,purified recombinant proteins or cellular lysates.Reactions were terminated after 10,30 or 60 min of incubation with Doles medium.RESULTS:Expression of soluble recombinant ACSL proteins was found after incubation with isopropyl betaD-1-thiogalactopyranoside and after ultracentrifugation these were further purified to near homogeneity with Ni 2+-affinity chromatography.Triacsin C selectively and strongly inhibited recombinant human ACSL5 protein at pH 7.5 and pH 9.5,as well as recombinant rat ACSL1(sensitive control),but not recombinant rat ACSL5(insensitive control).The IC50 for human ACSL5 was about 10 μmol/L.The inhibitory triacsin C effect was similar for different incubation times(10,30 and 60 min) and was not modified by the N-or C-terminal location of the 6xHis-tag.In order to evaluate ACSL5 sensitivity to triacsin C in a cellular environment,stable human ACSL5 CaCo2 transfectants and mechanically dissected normal human intestinal mucosa with high physiological expression of ACSL5 were analyzed.In both models,ACSL5 peak activity was found at pH 7.5 and pH 9.5,corresponding to the properties of recombinant human ACSL5 protein.In the presence of triacsin C(25 μmol/L),total ACSL activity was dramatically diminished in human ACSL5 transfectants as well as in ACSL5-rich human intestinal mucosa.CONCLUSION:The data strongly indicate that human ACSL5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms. | Elke Kaemmerer Anne Peuscher Andrea Reinartz Christian Liedtke Ralf Weiskirchen Jürgen Kopitz Nikolaus Gassler | 2011 | World Journal of Gastroenterology2011，17，44： | 3 |
| 19 | 复合技术制备锂二次电池电极材料显示文摘复合技术是进一步提高材料的物理化学性能和/或降低成本的有效方法之一,早就应用于锂二次电池中。综述了最近几年来复合技术在制备锂二次电池电极材料方面的进展。这些电极材料包括负极材料如碳基负极、锡基氧化物负极和新型的合金负极、以及无机和有机正极材料。复合的方法包括包覆、混合、沉积等。通过复合,提高了天然石墨的循环性能,降低了无定形碳在第1次循环的不可逆容量并改进了循环性能,改善了合金负极材料的循环寿命,明显提高了无机正极材料的高温性能及循环性能,并使有机正极材料的循环性能达到可实用化的水平。随着复合技术的不断发展,一些新的电极材料将不断诞生,其它类型锂二次电池的商品化将为期不远。 | 吴宇平 Rahm Elke Holze Rudolf | 2003 | 电池2003，33，1： | 3 |
| 20 | Near-infrared fluorescence guided esophageal reconstructive surgery: A systematic review显示文摘BACKGROUND After an esophagectomy, the stomach is most commonly used to restore continuity of the upper gastrointestinal tract. These esophago-gastric anastomoses are prone to serious complications such as leakage associated with high morbidity and mortality. Graft perfusion is considered to be an important predictor for anastomotic integrity. Based on the current literature we believe Indocyanine green fluorescence angiography(ICGA) is an easy assessment tool for gastric tube(GT) perfusion, and it might predict anastomotic leakage(AL).AIM To evaluate feasibility and effectiveness of ICGA in GT perfusion assessment and as a predictor of AL.METHODS This study was designed according to the PRISMA guidelines and registered in the PROSPERO database. PubMed and EMBASE were independently searched by 2 reviewers for studies presenting data on intraoperative ICGA GT perfusion assessment during esophago-gastric reconstruction after esophagectomy.Relevant outcomes such as feasibility, complications, intraoperative surgical changes based on ICGA findings, quantification attempts, anatomical data and the impact of ICGA on postoperative anastomotic complications, were collected by 2 independent researchers. The quality of the included articles was assessed based on the Methodological Index for Non-Randomized Studies. The 19 included studies presented data on 1192 esophagectomy patients, in 758 patients ICGA was used perioperative to guide esophageal reconstruction.RESULTS The 19 included studies for qualitative analyses all described ICGA as a safe and easy method to evaluate gastric graft perfusion. AL occurred in 13.8% of the entire cohort, 10% in the ICG guided group and 20.6% in the control group(P <0.001). When poorly perfused cases are excluded from the analyses, the difference in AL was even larger(AL well-perfused group 6.3% vs control group 20.5%, P <0.001). The AL rate in the group with an altered surgical plan based on the ICG image was 6.5%, similar to the well perfused group(6.3%) and significantly less than the poorly perfused group(47.8%)(P < 0.001), suggesting that the technique is able to identify and alter a potential bad outcome.CONCLUSION ICGA is a safe, feasible and promising method for perfusion assessment. The lower AL rate in the well perfused group suggest that a good fluorescent signal predicts a good outcome. | Elke Van Daele Yves Van Nieuwenhove Wim Ceelen Christian Vanhove Bart P Braeckman Anne Hoorens Jurgen Van Limmen Oswald Varin Dirk Van de Putte Wouter Willaert Piet Pattyn | 2019 | World Journal of Gastrointestinal Oncology2019，11，3： | 3 |
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