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| 1 | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases显示文摘Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water.In the gut flora,they activate azo pro-drugs,which are used for treatment of inflammatory bowel disease,releasing the active component 5-aminosalycilic acid.The bacterium P.aeruginosa has three azoreductase genes,paAzoR1,paAzoR2 and paAzoR3,which as recombinant enzymes have been shown to have different substrate specificities.The mechanism of azoreduction relies upon tautomerisation of the substrate to the hydrazone form.We report here the characterization of the P.aeruginosa azoreductase enzymes,including determining their thermostability,cofactor preference and kinetic constants against a range of their favoured substrates.The expression levels of these enzymes during growth of P.aeruginosa are altered by the presence of azo substrates.It is shown that enzymes that were originally described as azoreductases,are likely to act as NADH quinone oxidoreductases.The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution. | Ali Ryan Chan-Ju Wang Nicola Laurieri Isaac Westwood Edith Sim | 2010 | Protein & Cell2010，1，8： | 4 |
| 2 | Identification of arylamine N-acetyltransferase inhibitors as an approach towards novel anti-tuberculars显示文摘New anti-tubercular drugs and drug targets are urgently needed to reduce the time for treatment and also to identify agents that will be effective against Mycobacterium tuberculosis persisting intracellularly.Mycobacteria have a unique cell wall.Deletion of the gene for arylamine N-acetyltransferase(NAT)decreases mycobacterial cell wall lipids,particularly the distinctive mycolates,and also increases antibiotic susceptibility and killing within macrophage of Mycobacterium bovis BCG.The nat gene and its associated gene cluster are almost identical in sequence in M.bovis BCG and M.tuberculosis.The gene cluster is essential for intracellular survival of mycobacteria.We have therefore used pure NAT protein for high-throughput screening to identify several classes of small molecules that inhibit NAT activity.Here,we characterize one class of such molecules—triazoles—in relation to its effects on the target enzyme and on both M.bovis BCG and M.tuberculosis.The most potent triazole mimics the effects of deletion of the nat gene on growth,lipid disruption and intracellular survival.We also present the structure-activity relationship between NAT inhibition and effects on mycobacterial growth,and use ligand-protein analysis to give further insight into the structure-activity relationships.We conclude that screening a chemical library with NAT protein yields compounds that have high potential as anti-tubercular agents and that the inhibitors will allow further exploration of the biochemical pathway in which NAT is involved. | Isaac M.Westwood Sanjib Bhakta Angela J.Russell Elizabeth Fullam Matthew C.Anderton Akane Kawamura Andrew W.Mulvaney Richard J.Vickers Veemal Bhowruth Gurdyal S.Besra Ajit Lalvani Stephen G.Davies Edith Sim | 2010 | Protein & Cell2010，1，1： | 3 |
| 3 | Complement activation and protein adsorption by carbon nanotubes显示文摘 | Carolina Salvador-Morales Emmanuel Flahaut Edith Sim Jeremy Sloan Malcolm L. H. Green Robert B. Sim | 2005 | Molecular Immunology2005，，3： | 1 |
| 4 | Probing the architecture of the Mycobacterium marinum arylamine N-acetyltransferase active site显示文摘Treatment of latent tuberculosis infection remains an important goal of global TB eradication.To this end,targets that are essential for intracellular survival of Mycobacterium tuberculosis are particularly attractive.Arylamine N-acetyltransferase(NAT)represents such a target as it is,along with the enzymes encoded by the associated gene cluster,essential for mycobacterial survival inside macrophages and involved in cholesterol degradation.Cholesterol is likely to be the fuel for M.tuberculosis inside macrophages.Deleting the nat gene and inhibiting the NAT enzyme prevents survival of the microorganism in macrophages and induces cell wall alterations,rendering the mycobacterium sensitive to antibiotics to which it is normally resistant.To date,NAT from M.marinum(MMNAT)is considered the best available model for NAT from M.tuberculosis(TBNAT).The enzyme catalyses the acetylation and propionylation of arylamines and hydrazines.Hydralazine is a good acetyl and propionyl acceptor for both MMNAT and TBNAT.The MMNAT structure has been solved to 2.1Åresolution following crystallisation in the presence of hydralazine and is compared to available NAT structures.From the mode of ligand binding,features of the binding pocket can be identified,which point to a novel mechanism for the acetylation reaction that results in a 3-methyltriazolo[3,4-a]phthalazine ring compound as product. | Areej M.Abuhammad Edward D.Lowe Elizabeth Fullam Martin Noble Elspeth F.Garman Edith Sim | 2010 | Protein & Cell2010，1，4： | 1 |
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