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| 1 | Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays. | Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson | 2015 | World Journal of Gastroenterology2015，21，11： | 2 |
| 2 | SREBP transcription factors:master regulators of lipid homeostasis显示文摘 | HEGARTY B BOSSARD P | 2004 | Biochimie2004，86，11： | 1 |
| 3 | Controlled and renewable release of phosphorus in soils from mixtures of phosphate rock and ammonium-exchanged clinoptilolite显示文摘 | Lai T M Eberl D D | 1986 | Zeolites1986，6，： | 1 |
| 4 | SREBP transcription factors:master regulators of lipid homeostasis显示文摘 | Eberle D Hegarty B Bossard P | | 0，，： | 1 |
| 5 | Increased in- tegration of transplanted CD73-positive photoreceptor precur- sors into adult mouse retina 显示文摘 | Eberle D Schubert S Postel K Corbeil D Ader M | 2011 | Invest Ophthalmol Vis Sci2011，52，9： | 1 |
| 6 | SREBP transcription factors : mas- ter regulators of lipid homeostasis 显示文摘 | Eberle D Hegarty B Bossard P | 2004 | Bioehimie2004，86，11： | 1 |
| 7 | The nature of porosity in or- ganic-rich mudstones of the Upper Jurassic Kimmeridge Clay Formation, North Sea, offshore United Kingdom显示文摘 | Fishman N S Hackley P C Lowers H A Hill R J Egenhoff S O Eberl D D Blum A E | 2012 | Int J Coal Geol2012，7，12： | 1 |
| 8 | Critique of contingent valuation and travel cost methods for valuing natural resources and ecosystems显示文摘 | Eberle W D Hayden F G | 1991 | Journal of Economic Issues1991，，3： | 1 |
| 9 | Memory failure has different mechanisms in subcortical stroke and Alzheimer' s disease 显示文摘 | Reed BR Eberling JL Mungas D | 2000 | Ann Neurol2000，48，3： | 1 |
| 10 | Effects of white mat- ter lesions and lacunes on cortical function 显示文摘 | Reed BR Eberling JL Mungas D | 2004 | Arch Neurol2004，61，10： | 1 |
| 11 | Controlledand renewable release of phosphorus in soils from mixtures of phosphate rock and ammonium-exchanged clinoptiolite显示文摘 | Eberl D D | 1986 | Zealots1986，，6： | 1 |
| 12 | Effects of white matter lesions and lacunes on cortical function 显示文摘 | Reed BR Eberling JL Mungas D | 2004 | 61(10) : 1545-15502004，61，10： | 1 |
| 13 | Effects of white matter lesions and lacunes on cortical function显示文摘 | Reed BR Eberling JL Mungas D | 2004 | Arch Neurol2004，61，10： | 1 |
| 14 | XRD measurement of mean crystallite thickness of illite and illite/smectite: reapparaisal of the kubler index and the scherrer equation 显示文摘 | Drits V Srodon J Eberl D D | 1997 | Clays Clay Mineral1997，45，3： | 1 |
| 15 | Effects of white matter lesions and lacunes on cortical function显示文摘 | Reed BR Eberling JL Mungas D | 2004 | Arch Neurol2004，61，10： | 1 |
| 16 | SREBP transcription factors: master regulators of lipid homeo-stasis显示文摘 | Eberle D Hegarty B Bossard P Ferre P Foufelle F | 2004 | Biochimie2004，86，11： | 1 |
| 17 | Tag polymorphisms at the A20 ( TNFAIP3 ) locus are associated with lower gene expression and increased risk of coronary artery disease in type 2 diabetes 显示文摘 | Boonyasrisawat W Eberle D Bacci S | 2007 | Diabetes2007，56，2： | 1 |
| 18 | SREBP transcription factors: master regulators of lipid homeostasis 显示文摘 | Eberle D Hegarty B Bossard P Ferre P Foufelle F | 2004 | Biochimie2004，86，11： | 1 |
| 19 | Placental pathology in discordant twins 显示文摘 | EBERLE A M LEVESQUE D VINTZILEUS A M | 1993 | Am J Obstet Gynecol1993，169，： | 1 |
| 20 | Hydrothermal synthesis of ammonium illite显示文摘 | SUCHA V ELSASS F EBERL D D | 1998 | American Mineralogist1998，83，12： | 1 |
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