|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide concentrations on the mechanical and biological characteristics of cross-linked collagen fibres for tendon repair显示文摘Reconstituted type I collagen fibres have received considerable interest as tendon implant materials due to their chemical and structural similarity to the native tissue.Fibres produced through a semi-continuous extrusion process were cross-linked with different concentrations of the zerolength cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)in combination with N-hydroxysuccinimide(NHS).Tensile properties of the fibres were considered,along with imaging of both surface structure and fibrillar alignment.Resistance of the fibres to bacterial collagenase was investigated and fibre sections seeded with human tendon cells for biological characterization,including cell adhesion and proliferation.The work clearly demonstrated that whilst the concentration of EDC and NHS had no significant effect on the mechanics,a higher concentration was associated with higher collagenase resistance,but also provided a less attractive surface for cell adhesion and proliferation.A lower cross-linking concentration offered a more biocompatible material without reduction in mechanics and with a potentially more optimal degradability. | Zafar Ahmad Jennifer H.Shepherd David V.Shepherd Siddhartha Ghose Simon J.Kew Ruth E.Cameron Serena M.Best Roger A.Brooks John Wardale Neil Rushton | 2015 | Regenerative Biomaterials2015，2，2： | 2 |
| 2 | The central role of the P2T receptor in amplification of human platelet activation, aggregation, secretion and procoagulant activity显示文摘 | Robert F.Storey Heather M.Sanderson Ann E.White Jane A.May Kathryn E.Cameron StanHeptinstall | 2001 | British Journal of Haematology2001，，： | 1 |
| 3 | Targeted protein delivery:carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds显示文摘Tissue engineering response may be tailored via controlled,sustained release of active agents from protein-loaded degradable microparticles incorporated directly within three-dimensional(3D)ice-templated collagen scaffolds.However,the effects of covalent crosslinking during scaffold preparation on the availability and release of protein from the incorporated microparticles have not been explored.Here,we load 3D ice-templated collagen scaffolds with controlled additions of poly-(DL-lactide-co-glycolide)microparticles.We probe the effects of subsequent N-(3-dimethylaminopropyl)-N0-ethylcarbodiimide hydrochloride crosslinking on protein release,using microparticles with different internal protein distributions.Fluorescein isothiocyanate labelled bovine serum albumin is used as a model protein drug.The scaffolds display a homogeneous microparticle distribution,and a reduction in pore size and percolation diameter with increased microparticle addition,although these values did not fall below those reported as necessary for cell invasion.The protein distribution within the microparticles,near the surface or more deeply located within the microparticles,was important in determining the release profile and effect of crosslinking,as the surface was affected by the carbodiimide crosslinking reaction applied to the scaffold.Crosslinking of microparticles with a high proportion of protein at the surface caused both a reduction and delay in protein release.Protein located within the bulk of the microparticles,was protected from the crosslinking reaction and no delay in the overall release profile was seen. | Constantin Edi Tanase Omar Qutachi Lisa J.White Kevin M.Shakesheff Andrew W.McCaskie Serena M.Best Ruth E.Cameron | 2019 | Regenerative Biomaterials2019，6，5： | 1 |
| 4 | Very high resolution interpolated climate surfaces for global land areas显示文摘 | Robert J.Hijmans Susan E.Cameron Juan L.Parra Peter G.Jones AndyJarvis | 2005 | Int J Climatol2005，，15： | 1 |
| 5 | Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems显示文摘CRISPR/Cas-mediated genome editing in human pluripotent stem cells(hPSCs)offers unprecedented opportunities for developing in vitro disease modeling,drug screening and cell-based therapies.To efficiently deliver the CRISPR components,here we developed two all-in-one vectors containing Cas9/gRNA and inducible Cas13d/gRNA cassettes for robust genome editing and RNA interference respectively.These vectors utilized the PiggyBac transposon system,which allows stable expression of CRISPR components in hPSCs.The Cas9 vector PB-CRISPR exhibited high efficiency(up to 99%)of inducing gene knockout in both protein-coding genes and long non-coding RNAs.The other inducible Cas13d vector achieved extremely high efficiency in RNA knockdown(98%knockdown for CD90)with optimized gRNA designs.Taken together,our PiggyBac CRISPR vectors can serve as powerful toolkits for studying gene functions in hPSCs. | Yuqian Jiang Rachel Catherine Hoenisch Yun Chang Xiaoping Bao Craig E.Cameron Xiaojun Lance Lian | 2022 | Bioactive Materials2022，7，8： | 0 |
| 6 | Avoiding artefacts in MicroCT imaging of collagen scaffolds:Effect of phosphotungstic acid(PTA)-staining and crosslink density显示文摘X-ray micro-computed tomography(μ-CT)can be used to provide both qualitative and quantitative information on the structure of three-dimensional(3D)bioactive scaffolds.When performed in a dry state,μ-CT accurately reflects the structure of collagen-based scaffolds,but imaging in a wet state offers challenges with radiolucency.Here we have used phosphotungstic acid(PTA)as a contrast agent to visualise fully hydrated collagen scaffolds in a physiologically relevant environment.A systematic investigation was performed to understand the effects of PTA on the results of μ-CT imaging by varying sample processing variables such as crosslinking density,hydration medium and staining duration.Immersing samples in 0.3% PTA solution overnight completely stained the samples and the treatment provided a successful route forμ-CT analysis of crosslinked samples.However,significant structural artefacts were observed for samples which were either non-crosslinked or had low levels of crosslinking,which had a heterogeneous interior architecture with collapsed pores at the scaffold periphery.This work highlights the importance of optimising the choice of processing and staining conditions to ensure accurate visualisation for hydrated 3D collagen scaffolds in an aqueous medium. | Kyung-Ah Kwon Daniel V.Bax Jennifer H.Shepherd Ruth E.Cameron Serena M.Best | 2022 | Bioactive Materials2022，7，2： | 0 |
| 7 | 2000~2015年非洲疟疾防治措施对恶性疟原虫的影响显示文摘自2000年以来,在撒哈拉以南非洲地区的抗疟行动干预覆盖率达到前所未有的水平,了解其控制效果对指导下一步控制措施的制定至关重要。然而由于监测方法较为简单和缺乏可靠的监测数据,非洲不同流行模式下疟疾干预措施对疾病发病水平的影响仍然知之甚少。本研究根据疟疾现场调查的大量数据,重建从2000年至2015年的干预措施覆盖率变化趋势,并量化疟疾防控工作对其影响。研究结果显示从2000年至2015年,非洲恶性疟原虫带虫率减半,发病率下降了40%,自2000年以来干预措施已经避免了6.63(5.42—7.53可信区间)亿的临床病例。经杀虫剂处理过的蚊帐作为最广泛的干预措施,为降低疾病发病做出最大的贡献率(避免了68%的发病)。尽管干预措施的效果仍然低于预期水平,目前的疟疾干预措施已明显降低了整个非洲大陆的疟疾发病率。2015年之后的控制策略应以继续加强目前的干预措施,并保持杀虫剂有效性为基础。 | S.Bhatt E.Cameron 邓舒 刘永孝 | 2016 | 安徽预防医学杂志2016，0，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员