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| 1 | Effects of undigested protein-rich ingredients on polarised small intestinal organoid monolayers显示文摘Here, we describe the use of monolayers of intestinal epithelial cells derived from intestinal organoids and transcriptomics to investigate the direct effects of dietary protein sources on epithelial function. Mechanically dissociated 3 D organoids of mouse duodenum were used to generate a polarized epithelium containing all cell types found in the tissue of origin. The organoid-derived cell monolayers were exposed to 4%(w/v) of ‘undigested(non-hydrolysed)-soluble' fraction of protein sources used as feed ingredients [soybean meal(SBM) and casein], or alternative protein sources(spray dried plasma protein, and yellow meal worm), or controls for 6 h prior to RNA isolation and transcriptomics. All protein sources altered expression of unique biological processes in the epithelial cells. Exposure of intestinal organoids to SBM downregulated expression of retinol and retinoid metabolic processes as well as cholesterol and lipid biosynthetic pathways, consistent with the reported hypotriglyceridaemic effect of soy protein in vivo. These findings support the use of intestinal organoids as models to evaluate complex interactions between dietary ingredients and the intestinal epithelium and highlights some unique host effects of alternative protein sources in animal feed and potentially human food. | Soumya K.Kar Bart van der Hee Linda M.P.Loonen Nico Taverne Johanna J.Taverne-Thiele Dirkjan Schokker Mari A.Smits Alfons J.M.Jansman Jerry M.Wells | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 3 |
| 2 | 'Experimental Study of the Impact of an Ink-Jet Printed Droplet on a Solid Substrate'显示文摘 | Van Dam Dirkjan B Le Clerc Chritophe | 2004 | Physics of Fluids2004，16，9： | 1 |
| 3 | Cyclosporin A reduces CD4 + CD25 + regulatory T-cell numbers in patients with atopic dermatitis显示文摘 | DirkJan Hijnen Inge Haeck Annemieke A. van Kraats Evert Nijhuis Marjolein S. de Bruin-Weller Carla A.F.M. Bruijnzeel-Koomen Edward F. Knol | 2009 | The Journal of Allergy and Clinical Immunology2009，，： | 1 |
| 4 | The predictive value of anti - cyclic eitrulli- nated peptide antibodies in early arthritis显示文摘 | Jansen Louise M A van Sehaardertburg Dirkjan van der Horst - Bruinsma Irene | 2003 | Journal of Rheumatol- ogy2003，30，8： | 1 |
| 5 | Relationship among shoulder proprioeeption, kinematics, and pain after stroke显示文摘 | MARTIJN H NIESSEN DIRKJAN H | 2009 | Arch Phys Med Rehabil2009，90，： | 1 |
| 6 | The prognstic value of anti-cyclic citrullinated peptide(CCP)antibodies in early polyarthritis显示文摘 | Annemarie LMA Jansen Dirkjan van Schaardenburg Irene E van derHor-st-Bruinsma | 2001 | Arthritis Rheum2001，44，9： | 1 |
| 7 | Dietary supplementation of zinc oxide modulates intestinal functionality during the post-weaning period in clinically healthy piglets显示文摘Background To improve our understanding of host and intestinal microbiome interaction,this research investigated the effects of a high-level zinc oxide in the diet as model intervention on the intestinal microbiome and small intestinal functionality in clinically healthy post-weaning piglets.In study 1,piglets received either a high concentration of zinc(Zn)as zinc oxide(Zn O,Zn,2,690 mg/kg)or a low Zn concentration(100 mg/kg)in the diet during the post weaning period(d 14–23).The effects on the piglet's small intestinal microbiome and functionality of intestinal tissue were investigated.In study 2,the impact of timing of the dietary zinc intervention was investigated,i.e.,between d 0–14 and/or d 14–23 post weaning,and the consecutive effects on the piglet's intestinal functionality,here referring to microbiota composition and diversity and gene expression profiles.Results Differences in the small intestinal functionality were observed during the post weaning period between piglets receiving a diet with a low or high concentration Zn O content.A shift in the microbiota composition in the small intestine was observed that could be characterized as a non-pathological change,where mainly the commensals inter-changed.In the immediate post weaning period,i.e.,d 0–14,the highest number of differentially expressed genes(DEGs)in intestinal tissue were observed between animals receiving a diet with a low or high concentration Zn O content,i.e.,23 DEGs in jejunal tissue and 11 DEGs in ileal tissue.These genes are involved in biological processes related to immunity and inflammatory responses.For example,genes CD59 and REG3G were downregulated in the animals receiving a diet with a high concentration Zn O content compared to low Zn O content in both jejunum and ileum tissue.In the second study,a similar result was obtained regarding the expression of genes in intestinal tissue related to immune pathways when comparing piglets receiving a diet with a high concentration Zn O content compared to low Zn O content.Conclusions Supplementing a diet with a pharmaceutical level of Zn as Zn O for clinically healthy post weaning piglets influences various aspects intestinal functionality,in particular in the first two weeks post-weaning.The model intervention increased both the alpha diversity of the intestinal microbiome and the expression of a limited number of genes linked to the local immune system in intestinal tissue.The effects do not seem related to a direct antimicrobial effect of Zn O. | Dirkjan Schokker Soumya K.Kar Els Willems Alex Bossers Ruud A.Dekker Alfons J.M.Jansman | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
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