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| 1 | Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma显示文摘Objective:Myeloma bone disease(MBD)is the most common complication of multiple myeloma(MM).Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone disease.However,the mechanism of C3 a/C4 a in osteoclasts MM patients remains unclear.Methods:The formation and function of osteoclasts were analyzed after adding C3 a/C4 a in vitro.RNA-seq analysis was used to screen the potential pathways affecting osteoclasts,and the results were verified by Western blot,q RT-PCR,and pathway inhibitors.Results:The osteoclast area per view induced by 1μg/m L(mean±SD:50.828±12.984%)and 10μg/m L(53.663±12.685%)of C3 a was significantly increased compared to the control group(0μg/m L)(34.635±8.916%)(P<0.001 and P<0.001,respectively).The relative m RNA expressions of genes,OSCAR/TRAP/RANKL/cathepsin K,induced by 1μg/m L(median:5.041,3.726,1.638,and 4.752,respectively)and 10μg/m L(median:5.140,3.702,2.250,and 5.172,respectively)of C3 a was significantly increased compared to the control group(median:3.137,2.004,0.573,and 2.257,respectively)(1μg/m L P=0.001,P=0.003,P<0.001,and P=0.008,respectively;10μg/m L:P<0.001,P=0.019,P<0.001,and P=0.002,respectively).The absorption areas of the osteoclast resorption pits per view induced by 1μg/m L(mean±SD:51.464±11.983%)and 10μg/m L(50.219±12.067%)of C3 a was also significantly increased(33.845±8.331%)(P<0.001 and P<0.001,respectively)compared to the control.There was no difference between the C4 a and control groups.RNA-seq analysis showed that C3 a promoted the proliferation of osteoclasts using the phosphoinositide 3-kinase(PI3 K)signaling pathway.The relative expressions of PIK3 CA/phosphoinositide dependent kinase-1(PDK1)/serum and glucocorticoid inducible protein kinases(SGK3)genes and PI3 K/PDK1/p-SGK3 protein in the C3 a group were significantly higher than in the control group.The activation role of C3 a in osteoclasts of MM patients was reduced by the SGK inhibitor(EMD638683).Conclusions:C3 a activated osteoclasts by regulating the PI3 K/PDK1/SGK3 pathways in MM patients,which was reduced using a SGK inhibitor.Overall,our results identified potential therapeutic targets and strategies for MBD patients。 | Fengjuan Jiang Hui Liu Fengping Peng Zhaoyun Liu Kai Ding Jia Song Lijuan Li Jin Chen Qing Shao Siyang Yan Kim De Veirman Karin Vanderkerken Rong Fu | 2021 | Cancer Biology & Medicine2021，18，3： | 3 |
| 2 | Electrodeposition Process for the Fabrication of Copper Dendrites Film with Stable Superhydrophobicity显示文摘Copper dendrites on the copper surface were successfully prepared by electrodeposition in acidic copper sulfate aqueous solution containing ethanol.The XRD(X-ray difraction)patterns indicate that the Cu dendrites possess fcc(face-centered cubic)crystal structure.The contact angle of the as-prepared Cu dendrites surface without palmitic acid modification is almost 0 and the surface is completely wetted by water.After modified with palmitic acid,the Cu dendrite surface shows superhydrophobicity with a contact angle of 160.The polarization curves reveal that the superhydrophobic Cu dendrites surface exhibits a distinct passivation phenomenon,which could provide enhanced corrosion resistance for the substrate in the aqueous solution.The weight loss measurements show that the corrosion values of superhydrophobic surface is much lower than that of the bare copper,further meaning that the as-prepared surface has the anticorrosion performance. | Huan SHEN Fengping WANG Yanwei DING Jun YANG | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，6： | 2 |
| 3 | Concentration of Alkal ine Pectic Lyase with Ultrafiltration Process显示文摘 | Ding Fengping Hidetaka Noritomi Kunio Nagahama | 2001 | Membrane Science and Technology2001，，6： | 1 |
| 4 | Pd electrocatalyst supported on carbonized TiO 2 nanotube for ethanol oxidation显示文摘 | Fengping Hu Fanwu Ding Shuqin Song Pei Kang Shen | 2006 | Journal of Power Sources2006，，1： | 1 |
| 5 | Refractory humic-like dissolved organic matter fuels microbial communities in deep energy-limiting marine sediments显示文摘Humic-like dissolved organic matter(DOM),usually regarded as refractory,is a major component of DOM in marine sediment pore waters.However,its bio-reactivity remains poorly explored in natural environments,which makes its roles in supporting subsurface microbial communities and regulating long-term carbon cycling elusive.Here,the bio-reactivity of humiclike DOM was evaluated by modeled reaction rates together with its interactions with microbial communities in five sediment cores collected from the eutrophic Pearl River Estuary to the oligotrophic deep-sea basin in the northern South China Sea.We revealed contrasting relationships between humic-like DOM and microbes in the coastal and deep-sea sediments.In eutrophic coastal sediments,specific microbial groups enriched in the deep layers co-varied with humic-like DOM,while most microbial groups were significantly correlated with protein-like DOM,microbial transformation of which likely resulted in the production of humic-like DOM.On the contrary,in energy-limiting deep-sea sediments,over 70%of the microbial groups were found closely correlated with humic-like DOM,a net consumption of which was demonstrated in deep layers.The consumption of humic-like DOM in deep-sea sediments reduced its total production flux in the uppermost~5-meter layer to about one-tenth of that in coastal sediments,which could consequently decrease the refractory DOM flux to the overlying seawater and influence long-term oceanic carbon cycling. | Yunru CHEN Weikang SUI Jing WANG Ding HE Liang DONG Joanna JWANIEK Fengping WANG | 2023 | Science China Earth Sciences2023，66，8： | 1 |
| 6 | Concentration of alkal ine pectic lyase with ultrafilitration process显示文摘 | Ding Fengping Hidetaka Noritomi Kunio Nagahama | 2001 | Membrane Science and Technology2001，，6： | 1 |
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