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| 1 | α-MSH inhibits TNF-α-induced maturation of human dendritic cells in vitro through the up-regulation of ANXA1显示文摘-Melanocyte-stimulating 荷尔蒙(-MSH), 一反煽动性并且 immunomodulatory neuropeptide,被显示了在自体免疫的疾病和 allograft 拒绝的试验性的治疗有效。然而,它的规章的机制仍然是不清楚的。成熟树枝状的房间(DC ) 是有免疫力的反应和发炎的枢轴的开始者。我们假设了在 DC 成熟的 -MSH 的规章的角色将在 immune-response-mediated 疾病贡献 -MSH 的效果,这当模特儿。-MSH 禁止了导致的肿瘤坏死 factor-alpha (TNF-) ,这被发现人的 peripheral-monocyte-derived DC (MoDCs ) 的成熟, phenotypically 并且机能上地。这通过 co-stimulatory 分子 CD83 的表示的下面规定发生了,建议由 -MSH 的 DC 成熟的抑制能贡献这 neuropeptide 的反煽动性的效果并且 CD86, IL-12 的生产, IL-10 分泌物的提升,和 MoDC 吞噬细胞的活动。而且,增加了 A1 (ANXA1 ) 被发现为在 TNF 上禁止效果的 -MSH 负责的 annexin 的表示 -- 导致的 MoDC 成熟,能被 MoDCs 的处理与指向 ANXA1 (ANXA1-siRNA ) 的特定的、小介入的 RNA 废除,建议那 -MSH-induced ANXA1 调停抑制。因此, -MSH 禁止 TNF -- 通过 -MSH-up-regulated ANXA1 的人的 DC 的导致的成熟,由 -MSH 建议 DC 的成熟的那抑制能调停 neuropeptide 的反煽动性的效果。而且, ANXA1 能在调停免疫者的煽动性的疾病基于 DC 的角色作为一个新治疗学的药目标被识别。 | Yan Min Deping Han Zhanjiang Fu Honghai Wang Lirong Liu Yeping Tian | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 3 |
| 2 | Genome-wide profiling of RNA editing sites in sheep显示文摘Background: The widely observed RNA-DNA differences(RDDs) have been found to be due to nucleotide alteration by RNA editing. Canonical RNA editing(i.e., A-to-I and C-to-U editing) mediated by the adenosine deaminases acting on RNA(ADAR) family and apolipoprotein B mRNA editing catalytic polypeptide-like(APOBEC)family during the transcriptional process is considered common and essential for the development of an individual.To date, an increasing number of RNA editing sites have been reported in human, rodents, and some farm animals;however, genome-wide detection of RNA editing events in sheep has not been reported. The aim of this study was to identify RNA editing events in sheep by comparing the RNA-seq and DNA-seq data from three biological replicates of the kidney and spleen tissues.Results: A total of 607 and 994 common edited sites within the three biological replicates were identified in the ovine kidney and spleen, respectively. Many of the RDDs were specific to an individual. The RNA editing-related genes identified in the present study might be evolved for specific biological functions in sheep, such as structural constituent of the cytoskeleton and microtubule-based processes. Furthermore, the edited sites found in the ovine BLCAP and NEIL1 genes are in line with those in previous reports on the porcine and human homologs, suggesting the existence of evolutionarily conserved RNA editing sites and they may play an important role in the structure and function of genes.Conclusions: Our study is the first to investigate RNA editing events in sheep. We screened out 607 and 994 RNA editing sites in three biological replicates of the ovine kidney and spleen and annotated 164 and 247 genes in the kidney and spleen, respectively. The gene function and conservation analysis of these RNA editing-related genes suggest that RNA editing is associated with important gene function in sheep. The putative functionally important RNA editing sites reported in the present study will help future studies on the relationship between these edited sites and the genetic traits in sheep. | Yuanyuan Zhang Deping Han Xianggui Dong Jiankui Wang Jianfei Chen Yanzhu Yao Hesham Y.A.Darwish Wansheng Liu Xuemei Deng | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 2 |
| 3 | Molecular cloning and characterization of the endothelin 3 gene in black bone sheep显示文摘Background: Black bone sheep was first discovered in Yunnan province of China in 1970, with unique black pigmentation on the body and internal organs. Endothelin 3(EDN3) has been known as a key gene causing hyperpigmentation in black bone chicken, the Silky fowl.Methods: In this study, EDN3 was employed as a candidate gene for regulating black color pigmentation. First,EDN3 was cloned from sheep to obtain the full-length cDNA by using the rapid amplification of cDNA ends(RACE).Genomic EDN3 was screened and a total of thirty predicted single nucleotide polymorphisms(SNPs) were genotyped for allele and genotype frequency analysis in a case-control study involving two black bone sheep populations. Genomic copy number analysis of EDN3 in sheep was conducted to measure the variation in copy number. EDN3 expression levels were observed among the groups in adult liver, lymph node, and kidney tissues, as well as embryo kidney samples. Also, among the tissues of black bone and non-black bone sheep.Results: The size of the full-length cDNA was 1,578 bp, which included 426 bp of 5′-untranslated region(5′-UTR),an open reading frame(ORF) of 639 bp encoding a protein of 212 amino acids, and a 3′-UTR of 513 bp. Genotype and allele frequencies of all the discovered SNPs were found insignificantly different in black bone and non-black bone sheep(P > 0.05). Genomic copy number analysis of EDN3 in sheep revealed no significant difference between the two sheep groups. No significant variations were found in the adult liver and kidney embryo samples. However,the expression in lymph node and kidney tissue was significantly higher in black bone sheep than that in non-black bone sheep(P < 0.05). Significant variations in the EDN3 expression levels were observed among the tissues of nonblack bone sheep.Conclusions: The findings of the present study indicate that unlike in Silky chickens, EDN3 is not responsible for hyperpigmentation but may play a key functional role in immune and excretory systems of black bone sheep. | Hesham Y.A.Darwish Yuanyuan Zhang Kai Cui Zu Yang Deping Han Xianggui Dong Huaming Mao Weidong Deng Xuemei Deng | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 2 |
| 4 | Serum decoy receptor 3, a potential new biomarker for sepsis显示文摘 | Yan-Qiang Hou Ping Xu Mei Zhang Deping Han Liang Peng Dong-Yu Liang Shanmin Yang Zhenhuan Zhang Jinsheng Hong Xiao-Li Lou Lurong Zhang Sunghee Kim | 2012 | 2012 (7-8)2012，，7： | 1 |
| 5 | A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl显示文摘Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes.Unlike the melanocytes that are confined to the integument in other vertebrates,melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration.In this study,we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl.Differential expression of 2517 microarray probes(P<0.01,Fold Change>2)was observed in Silky Fowl compared to White Leghorn.After filtration by cluster analysis,functional annotation and pathway analysis,eight differentially expressed genes were identified to be closely related to the development of melanocytes.Moreover,differences in expression of immune genes were also detected between Silky Fowl and White Leghorn.The differentially expressed genes associated with melanocyte development were verified by q-PCR,and results were highly consistent with the microarray data.The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl,although there were no big differences in melanoblast development between these two breeds.The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl. | Yulin LI Deping HAN Junying LI Dawn KOLTES Xuemei DENG | 2014 | Frontiers of Agricultural Science and Engineering2014，1，4： | 0 |
| 6 | Lactobacillus casei protects intestinal mucosa from damage in chicks caused by Salmonella pullorum via regulating immunity and the Wnt signaling pathway显示文摘This study is to explore the mechanisms underlying the protective effects of Lactobacillus casei(L.casei)on the intestinal mucosal barrier of chicks infected with Salmonella pullorum(S.pullorum)through histological,immunological,and molecular biology methods.The results showed that L.casei significantly reduced the diarrhea rate,increased the daily weight gain,and maintained normal levels of IgA,IgM,and IgG in the serum of chicks infected with S.pullorum.Furthermore,we found that L.casei markedly improved the immunity of gut mucosa by regulating cytokine and chemokine receptor balance,elevating the number of intraepithelial lymphocytes. | Deng Ziteng Han Deping Wang Yuying Wang Qiuzhen Ma Yunfei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 7 | Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization显示文摘Terminal deoxynucleotidyl transferase(Td T) has been characterized as template-independent polymerase using single-stranded DNA(ss DNA) as primers to generate random oligonucleotides. However, the extension performance of Td T to single-stranded RNA(ss RNA) is vague. By systematically comparing and contrasting the performance of Td T-catalyzed ss DNA and ss RNA extension, it is indicated that the catalytic efficiency of ss RNA as primers was about 3 times lower than ss DNA as primers. Collectively, it is believed that understanding the catalytic performance of Td T will help to design the strategy to synthesize chimeric DNA on 3-OH of ss RNA, which becomes invaluable. | Houyu Han Jingyu Cui Dianming Zhou Deping Hua Weipan Peng Mengyao Lin Yating Zhang Fangya Li Xiaoqun Gong Jianyu Zhang | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
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