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| 1 | Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics显示文摘几个抄写因素的宫外的表示能恢复胚胎的房间命运到有教养的体的房间并且产生导致的 pluripotent 干细胞(iPSCs ) ,揭示到 pluripotency 的一条以前未知的小径。然而,这种技术被低效率,慢动力学和多因素的要求当前限制。这里,我们证明 reprogramming 能被优化文化条件改进并且戏剧性地加速了。首先,我们开发了优化定义媒介, iCD1,它允许 Oct4/Sox2/Klf4 (OSK ) 完成的调停的 reprogramming 超离频效率(~10% 在白天 8 点) 。我们也发现这个优化条件使 Sox2 和 Klf4 变为非必需,尽管这二个因素的消除导致更低的效率和更慢的动力学。我们的研究定义一条弄短的线路,两个在预定和因素要求,向 pluripotency。这个新范例不仅提供一个基本原理进一步改进 iPSC 产生而且由定义因素简化 reprogramming 的概念的理解。 | Jiekai Chen Jing Liu You Chen Jiaqi Yang Jing Chen He Liu Xiangjie Zhao Kunlun Mo Hong Song Lin Guo Shilong Chu Deping Wang Ke Ding Duanqing Pei | 2011 | Cell Research2011，21，6： | 9 |
| 2 | Osteogenic and anti-tumor Cu and Mn-doped borosilicate nanoparticles for syncretic bone repair and chemodynamic therapy in bone tumor treatment显示文摘Critical bone defects caused by extensive excision of malignant bone tumor and the probability of tumor recurrence due to residual tumor cells make malignant bone tumor treatment a major clinical challenge.The present therapeutic strategy concentrates on implanting bone substitutes for defect filling but suffers from failures in both enhancing bone regeneration and inhibiting the growth of tumor cells.Herein,Cu and Mn-doped borosilicate nanoparticles(BSNs)were developed for syncretic bone repairing and anti-tumor treatment,which can enhance bone regeneration through the osteogenic effects of Cu^(2+) and Mn^(3+) ions and meanwhile induce tumor cells apoptosis through the hydroxyl radicals produced by the Fenton-like reactions of Cu^(2+) and Mn^(3+) ions.In vitro study showed that both osteogenic differentiation of BMSCs and angiogenesis of endothelial cells were promoted by BSNs,and consistently the critical bone defects of rats were efficiently repaired by BSNs through in vivo evaluation.Meanwhile,BSNs could generate hydroxyl radicals through Fenton-like reactions in the simulated tumor microenvironment,promote the generation of intracellular reactive oxygen species,and eventually induce tumor cell apoptosis.Besides,subcutaneous tumors of mice were effectively inhibited by BSNs without causing toxic side effects to normal tissues and organs.Altogether,Cu and Mn-doped BSNs developed in this work performed dual functions of enhancing osteogenesis and angiogenesis for bone regeneration,and inhibiting tumor growth for chemodynamic therapy,thus holding a great potential for syncretic bone repairing and anti-tumor therapy. | Libin Pang Renliang Zhao Jing Chen Jingxin Ding Xiaochen Chen Wenwen Chai Xu Cui Xiaolin Li Deping Wang Haobo Pan | 2022 | Bioactive Materials2022，7，6： | 1 |
| 3 | An efficient copper-catalyzed amination of aryl halides by aqueous am- monia显示文摘 | WANG Deping CAI Qian DING Ke | 2009 | AdvSynthCatal2009，351，1112： | 1 |
| 4 | The Microphysical Characteristics of Wintertime Cold Clouds in North China显示文摘The microphysical characteristics of wintertime cold clouds in North China were investigated from 22 aircraft observation flights from 2014 to 2017,2020,and 2021.The clouds were generated by mesoscale weather systems with little orographic component.Over the mixed-phase temperature range(–40℃to 0℃),the average fraction of liquid,mixedphase,and ice cloud was 4.9%,23.3%,and 71.8%,respectively,and the probability distribution of ice mass fraction was a half-U-shape,suggesting that ice cloud was the primary cloud type.The wintertime mixed-phase clouds in North China were characterized by large cloud droplet number concentration,small liquid water content(LWC),and small effective diameter of cloud droplets.The main reason for larger cloud droplet number concentration and smaller effective diameter of cloud droplets was the heavy pollution in winter in North China,while for smaller LWC was the lower temperature during flights and the difference in air mass type.With the temperature increasing,cloud droplet number concentration,LWC,and the size of ice particles increased,but ice number concentration and effective diameter of cloud droplets decreased,similar to other mid-latitude regions,indicating the similarity in the temperature dependence of cloud properties of mixed-phase clouds.The variation of the cloud properties and ice habit at different temperatures indicated the operation of the aggregation and riming processes,which were commonly present in the wintertime mixed-phase clouds.This study fills a gap in the aircraft observation of wintertime cold clouds in North China. | Xuexu WU Minghuai WANG Delong ZHAO Daniel ROSENFELD Yannian ZHU Yuanmou DU Wei ZHOU Ping TIAN Jiujiang SHENG Fei WANG Deping DING | 2022 | Advances in Atmospheric Sciences2022，39，12： | 1 |
| 5 | Characteristics and mechanism of smart fluid for sweep-controlling during CO_(2) flooding显示文摘A smart response fluid was designed and developed to overcome the challenges of gas channeling during CO_(2)flooding in low-permeability,tight oil reservoirs.The fluid is based on Gemini surfactant with self-assembly capabilities,and the tertiary amine group serves as the response component.The responsive characteristics and corresponding mechanism of the smart fluid during the interaction with CO_(2)/oil were studied,followed by the shear characteristics of the thickened aggregates obtained by the smart fluid responding to CO_(2).The temperature and salt resistance of the smart fluid and the aggregates were evaluated,and their feasibility and effectiveness in sweep-controlling during the CO_(2)flooding were confirmed.This research reveals:(1)Thickened aggregates could be assembled since the smart fluid interacted with CO_(2).When the mass fraction of the smart fluid ranged from 0.05%to 2.50%,the thickening ratio changed from 9 to 246,with viscosity reaching 13 to 3100 mPas.As a result,the sweep efficiency in low-permeability core models could be increased in our experiments.(2)When the smart fluid(0.5%to 1.0%)was exposed to simulated oil,the oil/fluid interfacial tension decreased to the level of 1×10^(-2)mN/m.Furthermore,the vesicle-like micelles in the smart fluid completely transformed into spherical micelles when the fluid was exposed to simulated oil with the saturation greater than 10%.As a result,the smart fluid could maintain low oil/fluid interfacial tension,and would not be thickened after oil exposure.(3)When the smart fluid interacted with CO_(2),the aggregates showed self-healing properties in terms of shear-thinning,static-thickening,and structural integrity after several shear-static cycles.Therefore,this fluid is safe to be placed in deep reservoirs.(4)The long-term temperature and salt resistance of the smart fluid and thickened aggregates have been confirmed. | XIONG Chunming WEI Falin YANG Haiyang ZHANG Song DING Bin LEI Zhengdong ZHANG Deping ZHOU Qiang | 2023 | Petroleum Exploration and Development2023，50，3： | 0 |
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