|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of rock dusting in preventing and reducing intensity of coal mine explosions显示文摘 | Yi Luo Deming Wang Jianwei Cheng | 2017 | International Journal of Coal Science & Technology2017，4，2： | 11 |
| 2 | miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta显示文摘Background: The goat(Caprahircus) is one of the most important livestock animals. Goat milk fat is an important component in the nutritional quality of goat milk. Growing evidence points to the critical roles of microRNAs(miRNAs) in lipid metabolism.Results: Using a highly sensitive method of S-poly(T) plus for miRNAs detection, we analyze the expression patterns of 715 miRNAs in goat mammary gland tissues at different stages of lactation. We observed that miR-25 expression had an inverse relationship with milk production. Overexpression of miR-25 significantly repressed triacylglycerol synthesis and lipid droplet accumulation. To explore the regulatory mechanism of miR-25 in milk lipid metabolism,we analyzed its putative target genes with bioinformatics analysis followed by 3′-UTR assays. Peroxisome proliferative activated receptor gamma coactivator 1 beta(PGC-1 beta), a key regulator of lipogenics was identified as a direct target of miR-25 with three specific sites within its 3′-UTR. In addition, miR-25 mimics in goat mammary epithelial cells reduced the expressions of genes involved in lipid metabolism.Conclusions: Taken together, our results show miR-25 is potentially involved in lipid metabolism and we reveal the function of the miR-25/PGC-1 beta regulatory axis during lactation. | Liuan Ma Huiling Qiu Zhi Chen Li Li Yan Zeng Jun Luo Deming Gou | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 9 |
| 3 | Isolation of Lymphocytes and Their Innate Immune Characterizations from Liver,Intestine,Lung and Uterus显示文摘In steady-state conditions, the number and distribution of lymphocyte populations are under homeostatic control.New lymphocytes are continuously produced in primary and secondary lymphoid organs and then achieve immune-competence within different tissues, and they must challenge with resident cells for survival. The first step in the study of tissue lymphoid cells is their isolation in intact and viable form appropriate for establishment of in vitro culture systems. For reasons of simplicity, cell purity, cell yields and various purposes, lymphocytes obtained from different tissues in different labs were subjected to diverse protocols. To fully elucidate the nature of the local immune system as well as to adequately study the innate role of lymphocytes in liver, intestine, lung and uterus, we briefly reviewed the characterization of resident lymphocytes, and additional information on those cells from non-lymphoid tissues by using the recommended operation procedure was also presented. | Jianhong Zhang Zhongjun Dong Rongbin Zhou Deming Luo Haiming Wei Zhigang Tian | 2005 | Cellular & Molecular Immunology2005，2，4： | 8 |
| 4 | Political connections, media monitoring and long-term loans显示文摘We analyze data on Chinese non-state-listed firms and find that it is easier for firms with political connections to obtain long-term loans with extended debt maturities than it is for firms without political connections. Our investigation indicates that this phenomenon is significantly less common with increased media monitoring. Houston et al.(2011) find strong evidence that the state ownership of media is associated with higher levels of bank corruption in China, but our study shows that, to a certain extent, media monitoring can curb corruption. | Deming Yang Zhengfei Lu Danglun Luo | 2014 | China Journal of Accounting Research2014，，3： | 4 |
| 5 | Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling显示文摘MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs in serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction (PCR)-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps: (1) sample collection and preparation; (2) global miRNAs profiling using quantitative real-time PCR (qRT-PCR); (3) data normalization and analysis; and (4) selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers. | Kang Kang Xiao Peng Jun Luo Deming Gou | 2012 | Journal of Animal Science and Biotechnology2012，3，2： | 3 |
| 6 | The return and loss of litter phosphorus in different types of sand dunes in Horqin Sandy Land,northeastern China显示文摘Litter phosphorus(P) return is important to maintain the P cycle and balance in the sandy land of arid areas.In this study,we determined the loss and return of litter P in sand dune areas and elucidated their relationship.We investigated litter production and litter P amount,and simulated leaf litter moving dynamics to understand the relationships between the loss of litter P and the total litter P,and between the return of litter P and the total litter P in active(AD),semi-stabilized(SSD) and stabilized(SD) dunes in Inner Mongolia,northeastern China.The vegetation litter P was 12.6,94.5,and 201.6 mg P/m2 in AD,SSD,and SD,respectively.A significant movement and loss of leaf litter P with time occurred on the three types of sand dunes.As a result,the loss of P was 7.4,46.9,and 69.8 mg P/m2 and the return of P was 5.5,47.6,and 131.8 mg P/m2 in AD,SSD,and SD,respectively.The relationship between both loss and return of P and total litter P in AD,SSD,and SD was revealed by linear regression.The slope of the regression line indicated the rate of loss or return of litter P.From AD to SD,the loss rate showed a declining slope(0.52,0.32,and 0.17 for AD,SSD,and SD,respectively),and the return rate showed a rising slope(0.48,0.67,and 0.83 for AD,SSD,and SD,respectively).The loss of litter P should be regarded in the local management of vegetation and land in sand dune areas.Improved vegetation restoration measures are necessary to decrease litter P loss to maintain the stability of ecosystems in sand dune areas. | QuanLai ZHOU DeMing JIANG ZhiMin LIU Alamusa XueHua LI YongMing LUO HongMei WANG | 2012 | Journal of Arid Land2012，4，4： | 3 |
| 7 | A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification显示文摘Background:Porcine reproductive and respiratory syndrome virus(PRRSV),and particularly its highly pathogenic genotype(HP-PRRSV),have caused massive economic losses to the global swine industry.Results:To rapidly identify HP-PRRSV,we developed a direct real-time reverse transcription polymerase chain reaction method(dRT-PCR) that could detect the virus from serum specimen without the need of RNA purification.Our dRT-PCR assay can be completed in 1.5 h from when a sample is received to obtaining a result.Additionally,the sensitivity of dRT-PCR matched that of conventional reverse transcription PCR(cRT-PCR) that used purified RNA.The lowest detection limit of HP-PRRSV was 6.3 TCID_(50) using dRT-PCR.We applied dRT-PCR assay to 144 field samples and the results showed strong consistency with those obtained by cRT-PCR.Moreover,the dRT-PCR method was able to tolerate 5-20%(v/v) serum.Conclusions:Our dRT-PCR assay allows for easier,faster,more cost-effective and higher throughput detection of HP-PRRSV compared with cRT-PCR methods.To the best of our knowledge,this is the first report to describe a real-time RT-PCR assay capable of detecting PRRSV in crude serum samples without the requirement for purifying RNA.We believe our approach has a great potential for application to other RNA viruses. | Kang Kang Keli Yang Jiasheng Zhong Yongxiang Tian Limin Zhang Jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou | 2015 | Journal of Animal Science and Biotechnology2015，6，1： | 2 |
| 8 | An improved Tet-on system in micro RNA overexpression and CRISPR/Cas9-mediated gene editing显示文摘Background: Tetracycline(Tet)-regulated expression system has become a widely applied tool to control gene activity. This study aimed to improve the Tet-on system with superior regulatory characteristics.Results: By comprehensively comparing factors of transactivators, Tet-responsive elements(TREs), orientations of induced expression cassette, and promoters controlling the transactivator, we developed an optimal Tet-on system with enhanced inducible efficiency and lower leakiness. With the system, we successfully performed effective inducible and reversible expression of micro RNA, and presented a more precise and easily reproducible fine-tuning for confirming the target of a mi RNA. Finally, the system was applied in CRISPR/Cas9-mediated knockout of nuclear factor of activated T cells-5(NFAT5), a protective transcription factor in cellular osmoregulation.Conclusions: This study established an improved Tet-on system for powerful and stringent gene regulation in functional genetic studies. | Kang Kang Lian Huang Qing Li Xiaoyun Liao Quanjin Dang Yi Yang Jun Luo Yan Zeng Li Li Deming Gou | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 2 |
| 9 | Structure, Pattern and Mechanisms of Formation of Seed Banks in Sand Dune Systems in Northeastern Inner Mongolia, China显示文摘 | Yan Qiaoling Liu Zhimin Zhu Jiaojun Luo Yongming Wang Hongmei Jiang Deming | 2005 | Plant and Soil (-)2005，，1： | 2 |
| 10 | Border tax adjustments in the climate policy context:CO2 versus broad-based GHG emission targeting显示文摘 | Madanmohan Ghosh Deming Luo Muhammad Shahid Siddiqui Yunfa Zhu | | 0，，： | 1 |
| 11 | Evaluation ofmonolaurin from camphor tree seeds for controlling foodspoilage fungi 显示文摘 | Chunyan Luo Zeling Zeng Deming Gong | 2014 | Food Control2014，46，： | 1 |
| 12 | Preparation, struc- ture and properties of nitrile-butadiene rubber-organoelay nano-composites by reactive mixing intercalation method 显示文摘 | Liu Lin Jia Deming Luo Yufang | 2006 | Ap- plied Polymer Science2006，100，3： | 1 |
| 13 | The use of zinc dimethacrylate functionalized graphene as a reinforcement in rubber composites显示文摘 | Yong Lin Yizhong Chen Ying Zhang Deming Jia Yuangfang Luo Lan Liu | 2015 | Polym. Adv. Technol2015，，5： | 1 |
| 14 | Microclimate Effect of Artificial Caragana microphylla Communities in Horqin Sandy Land显示文摘Caragana microphylla is one of the key species for vegetation restoration in Horqin Sandy Land. Adopting field investigation and outdoor experiments,the microclimate effect of artificial C. microphylla communities with different restoration years were studied by observing wind velocity,air temperature,relative humidity and soil temperature. The results show that:( 1) Caragana microphylla community has an obvious wind-breaking effect near ground surface. Compared with shifting dunes,the wind velocity in the 6-year-old and 11-year-old C. microphylla shrubs at the height of 30 cm separately decreases by 71. 9% and 76. 0%.( 2) Mean daily temperature in the 6-year-old and 11-yearold C. microphylla communities is 3. 7 ℃ and 4. 9 ℃ lower than in shifting dunes,respectively.( 3) The relative humidity of air in the Caragana microphylla shrubs is higher than in shifting dunes.( 4) Soil temperature in C. microphylla plantation is lower than in shifting dunes. These results are significant in further exploring material and energy exchange near surface layer of artificial vegetation in the extremely arid condition. | Shanfeng HE Lanlan QIU Deming JIANG Yongming LUO | 2014 | Asian Agricultural Research2014，6，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员