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| 1 | Novel pleiotropic loci controlling panicle architecture across environments in japonica rice (Oryza sativa L.)显示文摘To identify quantitative trait loci (QTLs) controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat (SSR) markers and 254 recombinant inbred lines (RILs) derived from a cross between cultivars Xiushui 79 and C Bao. Seven panicle traits were investigated under three environments. Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments. Percentage of phenotypic variation explained by single locus varied from 2% to 35%. Based on the mixed linear model, a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping, explaining 1.2%-35% of phenotypic variation. Among the 9 QTLs with more than 10% of explained phenotypic variation, two QTLs were for the number of primary branches per panicle (NPB), two for panicle length (PL), two for spikelet density (SD), one for the number of secondary branches per panicle (NSB), one for secondary branch distribution density (SBD), and one for the number of spikelets per panicle (NS), respectively. qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci, showing effects on PL and SD simultaneously. qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD, respec- tively. qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD, respectively. The C Bao alleles at the both QTLs showed positive effects on PL, and the Xiushui 79 alleles at the both QTLs showed positive effects on SD. Genetic variation of panicle traits are mainly attributed to additive effects. QTL × environment interactions were not significant for additive QTLs and additive × additive QTL pairs. | Yuan Guo Delin Hong | 2010 | Journal of Genetics and Genomics2010，37，8： | 13 |
| 2 | Solution structure of the RNA recognition domain of METTL3-METTL14 N^6-methyladenosine methyltransferase显示文摘N^6-methyladenosine(m6A),a ubiquitous RNA modification,is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between the methyltransferase domains(MTDs)of METTL3 and METTL14 has been previously determined.However,the MTDs alone possess no enzymatic activity.Here we present the solution structure for the zinc finger domain(ZFD)of METTL3,the inclusion of which fulfills the methyltransferase activity of METTL3-METTL14.We show that the ZFD specifically binds to an RNA containing 5'-GGACU-3'consensus sequence,but does not to one without.The ZFD thus serves as the target recognition domain,a structural feature previously shown for DNA methyltransferases,and cooperates with the MTDs of METTL3-METTL14 for catalysis.However,the interaction between the ZFD and the specific RNA is extremely weak,with the binding affinity at several hundred micromolar under physiological conditions.The ZFD contains two CCCH-type zinc fingers connected by an anti-parallel P-sheet.Mutational analysis and NMR titrations have mapped the functional interface to a contiguous surface.As a division of labor,the RNA-binding interface comprises basic residues from zinc finger 1 and hydrophobic residues fromβ-sheet and zinc finger 2.Further we show that the linker between the ZFD and MTD of METTL3 is flexible but partially folded,which may permit the cooperation between the two domains during catalysis.Together,the structural characterization of METTL3 ZFD paves the way to elucidate the atomic details of the entire process of RNA m6A modification. | Jinbo Huang Xu Dong Zhou Gong Ling-Yun Qin Shuai Yang Yue-Ling Zhu Xiang Wang Delin Zhang Tingting Zou Ping Yin Chun Tang | 2019 | Protein & Cell2019，10，4： | 11 |
| 3 | Multiple Time Scale Analysis of River Runoff Using Wavelet Transform for Dagujia River Basin, Yantai, China显示文摘Based on monthly river runoff and meteorological data, a method of Morlet wavelet transform was used to analyze the multiple time scale characteristics of river runoff in the Dagujia River Basin, Yantai City, Shandong Prov- ince. The results showed that the total annual river runoff in the Dagujia River Basin decreased significantly from 1966 to 2004, and the rate of decrease was 48×106m3/10yr, which was higher than the mean value of most rivers in China. Multiple time scale characteristics existed, which accounted for different aspects of the changes in annual river runoff, and the major periods of the runoff time series were identified as about 28 years, 14 years and 4 years with decreasing levels of fluctuation. The river runoff evolution process was controlled by changes in precipitation to a certain extent, but it was also greatly influenced by human activities. Also, for different time periods and scales, the impacts of cli- mate changes and human activities on annual river runoff evolution occurred at the same time. Changes in the annual river runoff were mainly associated with climate change before the 1980s and with human activities after 1981. | LIU Delin LILT Xianzhao LI Bicheng ZHAO Shiwei LI Xiguo | 2009 | Chinese Geographical Science2009，19，2： | 10 |
| 4 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 5 | O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N” rule显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2),the causative agent of coronavirus disease 2019(COVID-19),emerged in late 2019 and has since caused a pandemic.Although there have been extensive studies worldwide,our understanding of this newly emerged pathogen is far from sufficient.The pathogenesis of the SARS-CoV-2 infection is not fully understood,although a“two-stage”hypothesis was proposed in our previous study. | Wenmin Tian Delin Li Nan Zhang Guijie Bai Kai Yuan Haixia Xiao Feng Gao Yang Chen Catherine C.L.Wong George Fu Gao | 2021 | Cell Research2021，31，10： | 9 |
| 6 | Target-enclosing affine formation control of two-layer networked spacecraft with collision avoidance显示文摘This paper addresses a target-enclosing problem for multiple spacecraft systems by proposing a two-layer affine formation control strategy. Compared with the existing methods,the adopted two-layer network structure in this paper is generally directed, which is suitable for practical space missions. Firstly, distributed finite-time sliding-mode estimators and formation controllers in both layers are designed separately to improve the flexibility of the formation control system. By introducing the properties of affine transformation into formation control protocol design,the controllers can be used to track different time-varying target formation patterns. Besides, multilayer time-varying encirclements can be achieved with particular shapes to surround the moving target. In the sequel, by integrating adaptive neural networks and specialized artificial potential functions into backstepping controllers, the problems of uncertain Euler-Lagrange models, collision avoidance as well as formation reconfiguration are solved simultaneously. The stability of the proposed controllers is verified by the Lyapunov direct method. Finally, two simulation examples of triangle formation and more complex hexagon formation are presented to illustrate the feasibility of the theoretical results. | Yang XU Delin LUO Dongyu LI Yancheng YOU Haibin DUAN | 2019 | Chinese Journal of Aeronautics2019，32，12： | 9 |
| 7 | Genetic Mapping of Laminaria japonica and L. Iongissima Using Amplified Fragment Length Polymorphism Markers in a "Two-Way Pseudo- Testcross" Strategy显示文摘 | Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan | 2007 | Journal of Integrative Plant Biology2007，49，3： | 8 |
| 8 | Enhanced Vitamin C Production Mediated by an ABA-Induced PTP-like Nucleotidase Improves Plant Drought Tolerance in Arabidopsis and Maize显示文摘Abscisic acid(ABA)is a key phytohormone that mediates environmental stress responses.Vitamin C,or L-ascorbic acid(AsA),is the most abundant antioxidant protecting against stress damage in plants.How the ABA and AsA signaling pathways interact in stress responses remains elusive.In this study,we characterized the role of a previously unidentified gene,PTPN(PTP-like Nucleotidase)in plant drought tolerance.In Arabidopsis,(AtPTPN was expressed in multiple tissues and upregulated by ABA and drought treatments.Loss-of-function mutants oiAtPTPN were hyposensitive to ABA but hypersensitive to drought stresses,whereas plants with enhanced expression AtPTPN showed opposite phenotypes to.Overexpression of maize PTPN(ZmPTPN)promoted,while knockdown oiZmPTPN inhibited plant drought tolerance,indicating conserved and positive roles of PTPN in plant drought tolerance.We found that both AtPTPN and ZmPTPN release Pi by hydrolyzing GDP/GMP/dGMP/IMP/dIMP,and that AtPTPN positively regulated AsA production via endogenous Pi content control.Consistently,overexpression of VTC2,the rate-limiting synthetic enzyme in AsA biosynthesis,promoted AsA production and plant drought tolerance,and these effects were largely dependent on AtPTPN activity.Furthermore,we demonstrated that the heat shock transcription factor HSFA6a directly binds the AtPTPN promoter and activates AtPTPN expression.Genetic analyses showed that AtPTPN is required for HSFA6a to regulate ABA and drought responses.Taken together,our data indicate that PTPN-mediated crosstalk between the ABA signaling and AsA biosynthesis pathways positively controls plant drought tolerance. | Hui Zhang Yanli Xiang Neng He Xiangguo Liu Hongbo Liu Liping Fang Fei Zhang Xiaopeng Sun Delin Zhang Xingwang Li William Terzaghi Jianbing Yan Mingqiu Dai | 2020 | Molecular Plant2020，13，5： | 7 |
| 9 | Umbilical cord-derived mesenchymal stem cells regulate thymic epithelial cell development and function in Foxn 1^-/- mice显示文摘 | Guangyang Liu Lihua Wang Tianxiang Pang Delin Zhu Yi Xu Hanyu Wang Xiuli Cong Yongjun Liu | 2014 | Cellular & Molecular Immunology2014，11，3： | 6 |
| 10 | Effects of raw material extrusion and steam conditioning on feed pellet quality and nutrient digestibility of growing meat rabbits显示文摘This study was conducted to investigate the effects of raw material extrusion and steam conditioning on feed pellet quality and nutrient digestibility of growing meat rabbits, in order to determine appropriate rabbit feed processing methods and processing parameters. In Exp. 1, an orthogonal design was adopted.Barrel temperature, material moisture content and feed rate were selected as test factors, and acid detergent fiber(ADF) content was selected as an evaluation index to research the optimum extrusion parameters. In Exp. 2. a two-factor design was adopted. Four kinds of rabbit feeds were processed and raw material extrusion adopted optimum extrusion parameters of Exp. 1. A total of 40 healthy and 42-day-old rabbits with similar weight were used in a randomized design, which consisted of 4 groups and 10 replicates in each group(1 rabbits in each replicate). The adaptation period lasted for 7 d, and the digestion trial lasted for 4 d. The results showed as follows: 1) ADF was significantly affected by barrel temperature(P < 0.05); the optimum extrusion parameters were barrel temperature 125℃, moisture content 16% and feed rate 9 Hz. 2) Raw material extrusion and steam conditioning both significantly decreased powder percentage, pulverization ratio and protein solubility(P < 0.05), significantly improved hardness and starch gelatinization degree of rabbit feed(P < 0.05). They both had significant interaction effects on the processing quality of rabbit feed(P < 0.05). 3) Extrusion significantly improved the apparent digestibility of dry matter and total energy(P < 0.05). Extrusion and steam conditioning both significantly improved the apparent digestibility of crude fiber(CF), ADF and NDF(P < 0.05), but they had no interaction effects on the apparent digestibility of rabbit feed. Thus, using extrusion and steam conditioning technology at the same time in the weaning rabbits feed processing can improve the pellet quality and nutrient apparent digestibility of rabbit feed. | Kuoyao Liao Jingyi Cai Zhujun Shi Gang Tian Dong Yan Delin Chen | 2017 | Animal Nutrition2017，，2： | 6 |
| 11 | Affine formation maneuver tracking control of multiple second-order agents with time-varying delays显示文摘This paper considers an affine maneuver tracking control problem for leader-follower type second-order multi-agent systems in the presence of time-varying delays, where the interaction topology is directed. Using the property of the affine transformation,this paper gives the sufficient and necessary conditions of achieving the affine localizability and extends it to the second-order condition. Under the(n + 1)-reachable condition of the given n-dimensional nominal formation with n + 1 leaders, a formation of agents can be reshaped in arbitrary dimension by only controlling these leaders. When the neighboring positions and velocities are available, a formation maneuver tracking control protocol with time-varying delays is constructed with the form of linear systems, where the tracking errors of the followers can be specified. Based on Lyapunov-Krasovskii stability theory, sufficient conditions to realize affine maneuvers are proposed and proved, and the unknown control gain matrix can be solved only by four linear matrix inequalities independent of the number of agents. Finally, corresponding simulations are carried out to verify the theoretical results, which show that these followers can track the time-varying references accurately and continuously. | XU Yang LI DongYu LUO DeLin YOU YanCheng | 2019 | Science China(Technological Sciences)2019，62，4： | 5 |
| 12 | Genetics of psoriasis:a basis for precision medicine显示文摘Psoriasis is an inflammatory skin disease with a background of polygenic inheritance.Both environmental and genetic factors are involved in the etiology of the disease.In the last two decades,numerous studies have been conducted through linkage analysis,genome-wide association study(GWAS),and direct sequencing to explore the role of genetic variation in disease pathogenesis and progression.To date,>80 psoriasis susceptibility genes have been identified,including HLA-Cw6,IL12B,IL23R,and LCE3B/3C.Some genetic markers have been applied in disease prediction,clinical diagnosis,treatment,and new drug development,which could further explain the pathogenesis of psoriasis and promote the development of precision medicine.This review summarizes related research on genetic variation in psoriasis and explores implications of the findings in clinical application and the promotion of a personalized medicine project. | Delin Ran Minglong Cai Xuejun Zhang | 2019 | Precision Clinical Medicine2019，2，2： | 4 |
| 13 | Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing显示文摘Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase(ALS)gene to confer herbicide tolerance in rice.The four different missense mutations in the P171 codon,P171S,P171A,P171Yand P171F,exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors.For example,P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides.Interestingly,a novel triple mutant(P171F/G628E/G629S)had the highest tolerance to all five tested herbicides.Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron.Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties. | Rui Zhang Sha Chen Xiangbing Meng Zhuangzhuang Chai Delin Wang Yuge Yuan Kunling Chen Linjian Jiang Jiayang Li Caixia Gao | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 14 | Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var.Hangzhouwanensis显示文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity. | Yan Li Lingxin Kong Jufang Shen Qing Wang Qian Liu Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 4 |
| 15 | Coaxial-printed small-diameter polyelectrolyte-based tubes with an electrostatic self-assembly of heparin and YIGSR peptide for antithrombogenicity and endothelialization显示文摘Low patency ratio of small-diameter vascular grafts remains a major challenge due to the occurrence of thrombosis formation and intimal hyperplasia after transplantation.Although developing the functional coating with release of bioactive molecules on the surface of small-diameter vascular grafts are reported as an effective strategy to improve their patency ratios,it is still difficult for current functional coatings cooperating with spatiotemporal control of bioactive molecules release to mimic the sequential requirements for antithrombogenicity and endothelialization.Herein,on basis of 3D-printed polyelectrolyte-based vascular grafts,a biologically inspired release system with sequential release in spatiotemporal coordination of dual molecules through an electrostatic self-assembly was first described.A series of tubes with tunable diameters were initially fabricated by a coaxial extrusion printing method with customized nozzles,in which a polyelectrolyte ink containing of ε-polylysine and sodium alginate was used.Further,dual bioactive molecules,heparin with negative charges and Tyr-Ile-Gly-Ser-Arg(YIGSR)peptide with positive charges were layer-by-layer assembled onto the surface of these 3D-printed tubes.Due to the electrostatic interaction,the sequential release of heparin and YIGSR was demonstrated and could construct a dynamic microenvironment that was thus conducive to the antithrombogenicity and endothelialization.This study opens a new avenue to fabricate a small-diameter vascular graft with a biologically inspired release system based on electrostatic interaction,revealing a huge potential for development of small-diameter artificial vascular grafts with good patency. | Zhiwen Zeng Chengshen Hu Qingfei Liang Lan Tang Delin Cheng Changshun Ruan | 2021 | Bioactive Materials2021，6，6： | 3 |
| 16 | New advances in multiple autonomous aerial robots formation control technology显示文摘Autonomous aerial robotics has become a hot direction ofresearch inside the community of robotics and control. Theprimary problem addressed by formation control is to steermultiple aerial robots to form desired geometric patterns and,at the same time, realize desired collective swarming behaviorsin a decentralized or distributed manner. In contrast toground vehicles, aerial robots have the ability to work inthree-dimensional (3D) airspace. Equipped with electric orhydraulic motors, the vertical take-off and landing (VTOL)capability is a typical performance of aerial robots. Formationcontrol technology for such aerial robots is incessantlyspringing up to satisfy the requirements of highly intelligentautonomous systems, which affects both military and civilareas, including missile defense, battlefield surveillance,satellite network construction, fire suppression, power gridinspection, commercial show, etc. [1–5]. Such a problem ofmultiple aerial robots formation control is exceptionallychallenging to analyze if practical constraints such as complexdynamics, motion constraints, and imperfect measurementsare incorporated. | XU Yang LUO DeLin YOU YanCheng DUAN HaiBin | 2019 | Science China(Technological Sciences)2019，62，10： | 3 |
| 17 | Hierarchical protection control system of smart substations显示文摘This paper analyzes the problems of the relay protections and secondary circuits of traditional substations,and introduces the hierarchical protection and control system of smart substations based on the technologies like IEC 61850 Standard,electronic instrument transformer,process level network,and wide area optical fiber communication network,etc.The hierarchical protection and control system includes three levels:local area protection,substation area protection and control,and wide area protection and control.These three levels are combined to solve the problems of the relay protections in traditional substations,thus improving protection performance and control capability of the system. | Yuping ZHENG Delin WANG Zexin ZHOU Tuanjie CAO | 2014 | Journal of Modern Power Systems and Clean Energy2014，2，3： | 3 |
| 18 | Fate of tetracycline, sulfonamide and fluoroquinolone resistance genes and the changes in bacterial diversity during composting of swine manure显示文摘 | Ammaiyappan Selvam Delin Xu Zhenyong Zhao Jonathan W.C. Wong | 2012 | Bioresource Technology2012，，： | 3 |
| 19 | Affine transformation based formation maneuvering for discrete-time directed networked systems显示文摘For the tracking control problems of affine formation maneuvers with discrete leader-following multi-agent systems, various distributed control schemes based on the directed graphs are designed in this paper. Consider the single and double-integrator models, different types of formation maneuvers can be achieved subject to the states of leaders with the novel characteristic of affine localizability. Meanwhile, the properties of affine transformation and signed Laplacian are introduced, which simplify the structures of controllers with distinct constraints to a great extent. Moreover, the convergence analysis of proposed protocols is proven. Static, constant and time-varying target formations can be tracked successfully. In the end, corresponding numerical simulations are carried out to certify the validity of theoretical results. | XU Yang LUO DeLin YOU YanCheng DUAN HaiBin | 2020 | Science China(Technological Sciences)2020，63，1： | 3 |
| 20 | Distributed planar formation maneuvering of leader-follower networked systems via a barycentric coordinate-based approach显示文摘This paper presents a distributed planar leader-follower formation maneuver control strategy for multi-agent systems with different agent dynamic models.This method is based on the barycentric coordinate-based(BCB)control,which can be performed in the local coordinate frame of each agent with required local measurements.By exploring the properties of BCB Laplacians,a time-varying target formation can be BCB localizable by a sufficient number of leaders uniquely,and this formation is converted from a given nominal formation with geometrical similarity transformation.The proposed control laws can continuously maneuver collective single-and double-integrator agents to achieve a translation,scale,rotation,or even their compositions in various directions.For the formation shape control problem of multi-car systems with/without saturation constraints,the obtained control performance can preserve good robustness.Global stability is also proven by mathematical derivations and verified by numerical simulations. | XU Yang LUO DeLin DUAN HaiBin | 2021 | Science China(Technological Sciences)2021，64，8： | 2 |
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