|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Exploring the biological mechanism of qi deficiency syndrome with chronic obstructive pulmonary disease(COPD)based on integrated pharmacology显示文摘Objective:To explore the potential biological mechanism of chronic obstructive pulmonary disease(COPD)qi deficiency syndrome,we used the integrated pharmacology network computing platform and carried out experimental verification.Methods:Using an integrated pharmacology strategy to analyze the potential biological targets of COPD qi deficiency syndrome.Based on the established qi deficiency syndrome rat model of COPD,the biological targets of lung and skeletal muscle were detected by electron microscopy,adenosine triphosphate(ATP)content assays,and western blotting.Results:According to the integrated pharmacological results,it was found that the locations of cell components related to COPD qi deficiency syndrome were mainly mitochondria.Electron microscopy results using lung tissue showed that mitochondria in the lipopolysaccharide(LPS group)and pulmonary instillation of LPS combined with cigarette smoke(LPStCS group)were swollen,deformed,and fragmented,with disappearing or broken crista.Results also showed that the total content of ATP in the lung and skeletal muscle of both groups was significantly lower than that in the control group at the 12th week(P<.05).At the 12th week,the expression of dynamin-related protein 1(DRP1)and mitofusin 1(MFN1)protein was significantly difference than that of the control group(P<.05).At the 10th and 14th weeks,changes in fission and fusion proteins in mitochondria of the lung and skeletal muscle were further detected.There was also a significant difference in the expression between the two groups compared to that in the control group at the 10th week and 14th week(P<.05).Conclusion:These findings suggest that the changes in mitochondrial morphology and ATP content and the unbalanced expression of DRP1 and MFN1 might be the key mechanisms underlying qi deficiency syndrome in rats with COPD. | Dayu Lin Shuai Li Chunying Hou Xue Xu Shuzhen Guo Qingqing Wang | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，1： | 2 |
| 2 | 新型可遗传编码的荧光探针实现在果蝇、斑马鱼和小鼠中快速特异地示踪多巴胺显示文摘文章简介多巴胺是神经系统中一种重要的单胺类神经递质,它介导了一系列重要的生理、病理过程。如何在自由活动的动物脑中特异、精准地示踪特定时间、区域的多巴胺释放是长期以来困扰神经科学家的技术难题。 | 孙芳妙 曾健智 井淼 Jingheng Zhou 冯杰思 Scott F.Owen 骆奕辰 Funing Li 王欢 Takashi Yamaguchi 雍自昊 Yijing Gao Wanling Peng Lizhao Wang Siyu Zhang Jiulin Du Dayu Lin Min Xu Anatol C.Kreitzer Guohong Cui 李毓龙 | 2019 | 科学新闻2019，0，2： | 1 |
| 3 | Microstructure development, mechanical properties and formability of Mg–Zn–Y–Zr magnesium alloy显示文摘 | Qiang Chen Jun Lin Dayu Shu Chuankai Hu Zude Zhao Feng Kang Shuhai Huang Baoguo Yuan | 2012 | Materials Science & Engineering A2012，，： | 1 |
| 4 | Comparisons of microstructure, thixoformability and mechanical properties of high performance wrought magnesium alloys reheated from the as-cast and extruded states显示文摘 | Qiang Chen Baoguo Yuan Jun Lin Xiangsheng Xia Zude Zhao Dayu Shu | 2014 | Journal of Alloys and Compounds2014，，： | 1 |
| 5 | 可遗传编码的荧光探针用于在体快速特异地检测去甲肾上腺素显示文摘文章简介在人的神经系统中有数十亿个神经元,这些细胞主要通过神经递质的释放和感知完成相互之间的通讯。去甲肾上腺素(Norepinephrine,NE)是一种单胺类的神经递质,它作用于多种肾上腺素受体。在中枢神经系统和交感神经系统中都参与诸多必要的生理过程,如感觉信号的调节、注意力的调控、清醒与睡眠、学习与记忆等。 | 冯杰思 Changmei Zhang Julieta E.Lischinsky Miao Jing Jingheng Zhou Huan Wang Yajun Zhang Ao Dong Zhaofa Wu Hao Wu Weiyu Chen Peng Zhang Jing Zou S.Andrew Hires J.Julius Zhu Guohong Cui Dayu Lin Jiulin Du 李毓龙 | 2020 | 科学新闻2020，，2： | 0 |
| 6 | 6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13einduced BEAS-2B cell model显示文摘Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra chinensis(Turcz.)Baill.(Wu Wei Zi),respectivelydon an interleukin(IL)-13einduced BEAS-2B cell model in vitro.Methods:The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1%fetal bovine serum(FBS)in vitro.Mitoquinone mesylate(Mito-Q)treatment was used as a positive control group,and different concentrations of 6-gingerol,asarinin,and deoxyschizandrin were used to treat the models.The level of reactive oxygen species(ROS)production was detected by flow cytometry.The expression levels of LC3B,Beclin1,adenosine 50-monophosphate(AMP)eactivated protein kinase(AMPK),phosphory-lated-AMPeactivated protein kinase(P-AMPK),dynamin-related protein 1(DRP1),and mitochondrial fusion protein 2(MFN2)were detected by Western blot.Mitochondrial membrane potential(MMP)assay kit with JC-1 was utilized to detect the level of MMP.Results:The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1%FBS showed an increased ROS level and a decreased MMP.6-Gingerol,asarinin,and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model.Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B,while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model.Conclusion:6-Gingerol,asarinin,and deoxyschizandrin can reduce ROS generation and increase MMP,but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein.The three components have both synergistic and complementary effects in classic medicine compatibility.This study may provide an innovative strategy to reduce the lung inflammation related to IL-13. | Shuai Li Shuzhen Guo Dayu Lin Jiang Yu Jiayang Tang Xiaoxi lv Yuping Zhang Qingqing Wang | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员