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| 1 | 【特刊综述】利用雄激素受体敲除(ARKO)小鼠模型认识人类雄激素缺乏显示文摘雄激素的作用机理很复杂。最近,使用转基因修饰小鼠模型进行的雄激素受体研究使我们对雄激素作用机理有了更进一步的理解。Cre-loxP系统能够完成组织和/或细胞特异性的敲除。通过使用Cre—loxP系统已经获得了大量整体性或组织特异性雄激素受体(ARK0)敲除模型。这些ARKO模型的多个雄激素作用位点,包括心血管系统、免疫和造血系统、骨骼、肌肉、脂肪组织、前列腺和脑,均已被检测。本篇综述着重阐述了通过针对这些雄激素作用位点的ARKO小鼠模型而取得的人类雄激素缺乏的研究成果,并且指出充分理解Cre-loxPdx鼠模型的优缺点才能正确认识其表型。 | Kesha Rana Rachel A Davey Jeffrey D Zajac | 2014 | Asian Journal of Andrology2014，16，2： | 13 |
| 2 | PNPLA3、TM6SF2和HSD17B13变异体对普通人群肝硬化和肝癌患病风险的综合影响显示文摘【据Hepatology 2020年3月报道】PNPLA3、TM6SF2和HSD17B13变异体对普通人群肝硬化和肝癌患病风险的综合影响(作者Gellert-Kristensen H等)作者假设脂肪肝的遗传风险评分(GRS)可影响肝硬化和肝细胞癌(HCC)的患病风险。来自丹麦的Gellert-Kristensen等将3种遗传变异[含patatin样磷脂酶结构域蛋白3(PNPLA3)p.I148M;跨膜6,超家族成员2(TM6SF2)p.E167K;以及羟基类固醇17-β脱氢酶13(HSD17B13)rs72613567]组成危险因素评分(等位基因为0到6个). | 朱倩 牛俊奇 GELLERT-KRISTENSEN H RICHARDSON TG DAVEY SMITH G | 2020 | 临床肝胆病杂志2020，36，10： | 6 |
| 3 | 电击法诱导的欧白英原生质体转化和转化植株再生显示文摘本研究使用电击法成功地将带有标记基因NPTⅡ的质粒pCaMVNEO转入欧白英的原生质体,并获得了再生转化植株。通过用pDW2质粒进行的CAT基因短暂表达研究,确定了欧白英原生质体转化的电击条件为:电容30nF、电场强度1500V/cm、时间衰变常数59.4微秒;质粒DNA浓度为20μg/2×10~6原生质体。在以上条件下,欧白英原生质体的相对转化率为12.4%,绝对转化率为2.4×10^(-4)。在大多数抗性愈伤组织和从抗性愈伤组织再生的植株中检测到新霉素磷酸转移酶活性。分子杂交结果也证明了在转化植株中存在NPTⅡ基因序列,而未转化的对照植株则没有。 | 王国英 贺普超 Davey M.R. | 1994 | 生物工程学报1994，10，1： | 6 |
| 4 | 药物与喹诺酮类的相互作用显示文摘喹诺酮与其它药物的相互作用一般分药动学和药效学两种类型,现分述如下: 药动学的相互作用胃肠道吸收口服500mg环丙沙星(ciprofloxacin,)时同服含铝或镁的抗酸剂,血浓度可降低1/10到1/6,含钙的抗酸剂不影响环丙沙星的吸收。环丙沙星的吸收还可因促进胃肠道排空的药物甲氧氯普胺(胃复安)而加快,相反, | Davey PG 朱悦心 | 1989 | 国外医学(药学分册)1989，16，3： | 5 |
| 5 | 红菜薹原生质体培养植株再生显示文摘一、目的与材料方法 红菜薹属白菜类(B.campestris),其原生质体培养成功可供外源基因的直接转化和细胞质杂种合成雄性不育系之用。白菜类是十字花科中最难由原生质体再生出植株的种类之一(Hachey等,1991)。目前,大白菜的再生成株已有报道(李世君等,1992;Glimelius,1984),但再生频率低(3.3%,1%)。本试验试图建立起红菜薹原生质体有效的再生程序。 1.无菌苗的获得 红菜薹品种为十月红一号,种子在0.1%SDS的0.1%HgCl_2溶液中浸5分钟后,用15%的“Domestos”灭菌15分钟,再用无菌水洗5次,播种在MS基本培养基上发芽,25℃,光照每天16小时,光强1800lx。 | 叶志彪 李汉霞 Davey M.R. Power J.B. Cocking E.C. | 1993 | 园艺学报1993，20，4： | 5 |
| 6 | Management and outcome of recurrent gallstone ileus:A systematic review显示文摘AIM: To help the surgeon in decision making whentreating a patient with recurrent gallstone ileus(RGSI). METHODS: A systematic review related to RGSI was performed using the databases CINAHL, EMBASE, MEDLINE via Pub Med from May 1912 to April 2015. All languages were included and the grey literature was also searched. The abstracts were explored for relevance to the topic and full texts obtained as appropriate. A manual search was carried out by scrutinising the reference lists of all the full text articles and further articles were identified and obtained. Total of 903 articles were identified, 656 were excluded after abstract review, 247 full text articles were reviewed and 91 articles selected for final analysis. There were 113 cases of RGSI. RESULTS: There were 113 cases of RGSI reported in 91 articles. The majority of the recurrences, 62.6%, occurred within 6 wk of the index event. The male to female ratio was 1:7. The mean age was 69.6 years(SD 11.2) with a range of 38-95 years. The small bowel was the commonest site of impaction(92.2%). Treatment data was available for 104 patients. The two main operations performed were:(1) Enterolithotomy without repair of biliary fistula in 70.1% of all patients with a procedural mortality rate of 16.4%(12/73) and(2) a single stage surgery approach involving enterolithotomy with cholecystectomy and repair of the biliary enteric fistula in 16.3% with a procedural mortality of 11.7%(2/17). A subset analysis over last 25 years showed mortality from eneterolithotomy was 4.8% while single stage mortality was 22.2%. Enterolithotomy alone was the commonest operation performed for RGSI with four patients(5.4%) having a further recurrence of gallstone ileus. CONCLUSION: Enterolithotomy alone or followed by a delayed two-stage treatment approach is the preferred choice offering low mortality and reduced risk of recurrence. | Shovkat Ahmad Mir Zeiad Hussain Christine Ann Davey Glenn Vincent Miller Srinivas Chintapatla | 2015 | World Journal of Gastrointestinal Surgery2015，7，8： | 3 |
| 7 | 雄激素受体基因CAG多态性与前列腺癌的关系显示文摘目的 :进一步探讨雄激素受体 (AR)基因 CAG多态性与前列腺癌的关系。方法 :采用分子生物学方法对 35 8例前列腺癌患者 AR基因 CAG进行测试 ,分析 CAG长度与临床各种指标的关系。结果 :经统计学分析 ,显示 AR基因 CAG与年龄之间呈高相关性 (P =0 .0 0 7,γ=0 .114) ,但与 PSA、肿瘤分级及分期无显著相关性。结论 :AR基因 CAG的长度与患者年龄成正比 ,即前列腺癌患者年龄越轻 ,其 CAG长度越短。 | 陈越 Beilin J Zajac J D Favaloro J N Davey R Byron K Harewood H 唐涌志 孟荟 | 2001 | 临床泌尿外科杂志2001，16，2： | 3 |
| 8 | Hemodynamic monitoring in heart failure and pulmonary hypertension: From analog tracings to the digital age显示文摘Hemodynamic monitoring has long formed the cornerstone of heart failure(HF) and pulmonary hypertension diagnosis and management. We review the long history of invasive hemodynamic monitors initially using pulmonary artery(PA) pressure catheters in the hospital setting, to evaluating the utility of a number of implantable devices that can allow for ambulatory determination of intracardiac pressures. Although the use of indwelling PA catheters has fallen out of favor in a number of settings, implantable devices have afforded clinicians an opportunity for objective determination of a patient's volume status and pulmonary pressures. Some devices, such as the CardioM EMS and thoracic impedance monitors present as part of implantable cardiac defibrillators, are supported by a body of evidence which show the potential to reduce HF related morbidity and have received regulatory approval, whereas other devices have failed to show benefit and, in some cases, harm. Clearly these devices can convey a considerable amount of information and clinicians should start to familiarize themselves with their use and expect further development and refinement in the future. | Ryan Davey Amresh Raina | 2016 | World Journal of Transplantation2016，6，3： | 3 |
| 9 | Involvement of 90-kuD ribosomal S6 kinase in collagen type Ⅰ expression in rat hepatic fibrosis显示文摘AIM: To investigate the relationship between 90-kuD ribosomal S6 kinase (p90RSK) and collagen type □expression during the development of hepatic fibrosis in vivo and in vitro.METHODS: Rat hepatic fibrosis was induced by intraperitoneal injection of dimethylnitrosamine. The protein expression and cell location of p90RSK and their relationship with collagen type □were determined by co-immunofluoresence and confocal microscopy. Subsequently, RNAi strategy was employed to silence p90RSK mRNA expression in HSC-T6, an activated hepatic stellate cell (HSC) line. The expression of collagen type □in HSC-T6 cells was assessed by Western blotting and real-time polymerase chain reaction. Furthermore, HSCs were transfected with expression vectors or RNAi constructs of p90RSK to increase or decrease the p90RSK expression, then collagen type □promoter activity in the transfected HSCs was examined by reporter assay. Lastly HSC-T6 cells transfected with p90RSK siRNA was treated with or without platelet-derived growth factor (PDGF)-BB at a final concentration of 20 μg/L and the cell growth was determined by MTS conversion.RESULTS: In fibrotic liver tissues, p90RSK was overexpressed in activated HSCs and had a significant positive correlation with collagen type □levels.In HSC-T6 cells transfected with RNAi targeted to p90RSK, the expression of collagen type □was down-regulated (61.8% in mRNA, P < 0.01, 89.1% in protein, P < 0.01). However, collagen type □promoter activity was not increased with over-expression of p90RSK and not decreased with low expression either, compared with controls in the same cell line (P = 0.076). Furthermore, p90RSK siRNA exerted the inhibition of HSC proliferation, and also abolished the effect of PDGF on the HSC proliferation.CONCLUSION: p90RSK is over-expressed in activated HSCs and involved in regulating the abnormal expression of collagen type □through initiating the proliferation of HSCs. | Miao-Fang Yang Jun Xie Xiao-Yi Gu Xiao-Hua Zhang Andrew K Davey Shuang-Jie Zhang Ji-Ping Wang Ren-Min Zhu | 2009 | World Journal of Gastroenterology2009，15，17： | 3 |
| 10 | Establishment of Withania somnifera Hairy Root Cultures for the Production of Withanolide A显示文摘Withania sominifera (Indian ginseng) was transformed by Agrobacterium rhizogenes. Explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with A. rhizogenes strain R1601. Hairy (transformed) roots were induced from cotyledons and leaf explants. The transgenic status of hairy roots was confirmed by polymerase chain reaction using nptII and rolB specific primers and, subsequently, by Southern analysis for the presence of nptII and rolB genes in the genomes of transformed roots. Four clones of hairy roots were established; these differed in their morphology. The doubling time of faster growing cultures was 8-14 d with a fivefold increase in biomass after 28 d compared with cultured, non-transformed seedling roots. MS-based liquid medium was superior for the growth of transformed roots compared with other culture media evaluated (SH, LS and N6), with MS-based medium supplemented with 40 g/L sucrose being optimal for biomass production. Cultured hairy roots synthesized withanolide A, a steroidal lactone of medicinal and therapeutic value. The concentration of withanolide A in transformed roots (157.4 μg/g dry weight) was 2.7-fold more than in non-transformed cultured roots (57.9 μg/g dry weight). | Hosakatte N. Murthy Camelia Dijkstra Paul Anthony Daniel A. White Mike R. Davey J. Brian Power Eun J. Hahn Kee Y. Paek | 2008 | Journal of Integrative Plant Biology2008，50，8： | 3 |
| 11 | Organic mulching promotes soil organic carbon accumulation to deep soil layer in an urban plantation forest显示文摘Background:Soil organic carbon(SOC)is important for soil quality and fertility in forest ecosystems.Labile SOC fractions are sensitive to environmental changes,which reflect the impact of short-term internal and external management measures on the soil carbon pool.Organic mulching(OM)alters the soil environment and promotes plant growth.However,little is known about the responses of SOC fractions in rhizosphere or bulk soil to OM in urban forests and its correlation with carbon composition in plants.Methods:A one-year field experiment with four treatments(OM at 0,5,10,and 20 cm thicknesses)was conducted in a 15-year-old Ligustrum lucidum plantation.Changes in the SOC fractions in the rhizosphere and bulk soil;the carbon content in the plant fine roots,leaves,and organic mulch;and several soil physicochemical properties were measured.The relationships between SOC fractions and the measured variables were analysed.Results:The OM treatments had no significant effect on the SOC fractions,except for the dissolved organic carbon(DOC).OM promoted the movement of SOC to deeper soil because of the increased carbon content in fine roots of subsoil.There were significant correlations between DOC and microbial biomass carbon and SOC and easily oxidised organic carbon.The OM had a greater effect on organic carbon fractions in the bulk soil than in the rhizosphere.The thinnest(5 cm)mulching layers showed the most rapid carbon decomposition over time.The time after OM had the greatest effect on the SOC fractions,followed by soil layer.Conclusions:The frequent addition of small amounts of organic mulch increased SOC accumulation in the present study.OM is a potential management model to enhance soil organic matter storage for maintaining urban forest productivity. | Xiaodan Sun Gang Wang Qingxu Ma Jiahui Liao Dong Wang Qingwei Guan Davey L.Jones | 2021 | Forest Ecosystems2021，8，1： | 2 |
| 12 | A compreison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats显示文摘 | Winfield M Davey M R Karp A | 1993 | Heredity1993，70，： | 2 |
| 13 | A critical review of ferritin as a drug nanocarrier:Structure,properties,comparative advantages and challenges显示文摘Ferritin stores and releases iron ions in mammals.It is globally important as a drug nanocarrier.This is because of its unique hollow-spherical structure,desirable stability and biological properties.Novel drug-loading approaches plus various functionalization approaches have been developed to improve ferritin in response to differing demands in disease treatments.Here,we critically review ferritin drug delivery and evaluate its diverse drug-loading and functionalization approaches,we:(1)Introduce basic structural and property information related to ferritin as a drug nanocarrier;(2)Contrast in detail the different means to load drugs and the selection of drug loading means;(3)Discuss multiple ferritin functionalization approaches,together with related advantages and potential risks;and,(4)Compare ferritin with alternative,commonly-used drug nanocarriers.We conclude that despite that no drugs based on ferritin are commercially available,the market potential for it is significant,and evaluate future research directions.Findings from this work will be of immediate benefit and interest to a wide range of researchers and manufacturers for drug delivery using ferritin. | Shuang Yin Kenneth Davey Sheng Dai Yongdong Liu Jingxiu Bi | 2022 | Particuology2022，20，5： | 2 |
| 14 | 中低收入国家的慢性病:背景、决定因素与卫生政策显示文摘近年来,中低收入国家慢性病不断上升的趋势及其产生的影响受到广泛关注,但有关这种上升趋势的解释和说明大多基于高收入国家的慢性病发展史,但其所经历的发展历程与中低收入国家并不完全相同。本文对这些差异进行评价,以更好地理解中低收入国家慢性病的流行趋势,并对慢性病决定因素的相关理论加以讨论,为慢性病的防控提供循证支持。此外,本文将慢性病防控政策看作是降低人口患病风险和个体易感性的重要干预手段,并讨论了中低收入国家需要开展的相关政策研究。在此基础上进一步强调了加强初级卫生保健改革的必要性,并将其作为降低慢性病流行趋势的一项重要政策。 | Miranda J J Kinra S Casas J P Davey Smith G Ebrahim S | 2013 | 中国卫生政策研究2013，6，10： | 2 |
| 15 | Prognostic value of Her-2/neu and clinicopathologic factors for evaluating progression and disease-specific death in Chinese men with prostate cancer显示文摘背景 Her-2/neu 基因 overexpression 在几恶意被发现了,并且与差的预后被联系;当它在前列腺癌症( PCa )的 tumorigenesis 和前进的角色仍然是 controversial.This 时,学习试图为疾病前进和PCa特定的 death.Methods Her-2/neu 蛋白质表示与 PCa 在 对待antiandrogen 的中国人评估 Her-2/neu 蛋白质表示和 clinicopathologic 因素的预示的价值在收集的标本用 immunohistochemistry ( IHC )被决定从 124 前列腺活体检视和从七前列腺 canc 的前列腺( TURP ) | ZHANG Yi-fen GUAN Yang-bo YANG Bin WU Hong-yan DAI Yu-tian ZHANG Shuang-jie WANG Ji-ping Shailendra Anoopkumar-Dukie Andrew K Davey SUN Ze-yu | 2011 | Chinese Medical Journal2011，，24： | 2 |
| 16 | The relationship between steroidogenic factor I and DAX-I expression and in vitro gonadotropin secretion in human pituitaryadenomas 显示文摘 | Aylwin S J Welch J P Davey C L | 2001 | J Clin Endocrinol Metab2001，86，6： | 1 |
| 17 | Soft-tissue balancing of the hip: the role of femoral offset restoration显示文摘 | Charles MN Bourne RB Davey JR | 2005 | Instr Course Lect2005，54，： | 1 |
| 18 | Cloning, sequencing and expression analysis of the mouse NOD2/CARD15 gene 显示文摘 | Iwanaga Y Davey MP Martin TM | 2003 | Inflamm Res2003，52，： | 1 |
| 19 | Sequential antimicrobial therapy-the role of quinolones显示文摘 | Tillotson G Davey P | 1997 | J Antimicrob Chemother1997，39，： | 1 |
| 20 | Drug resistance does not correlate with resistance to Fas-mediated apoptosis 显示文摘 | Cullen KV Davey RA Davey MW | 2001 | Leuk Res2001，25，1： | 1 |
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