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1Transgenic expression of TaTLP1, a thaumatin-like protein gene, reduces susceptibility to common root rot and leaf rust in wheat显示文摘Thaumatin-like protein (TLP) plays an important role in combating plant pathogen infection.Common root rot caused by Bipolaris sorokiniana and leaf rust caused by Puccinia triticina (Pt) are major fungal diseases in wheat.The disease responses of TaTLP1-overexpressing transgenic lines (TaTLP1-OE) were evaluated after inoculation with each pathogen.The TaTLP1-OE lines had no apparent differences in tiller number and 1000-kernel weight from the wild type Jinan Wheat No.1 (JW1),whereas resistance to leaf rust and common root rot was improved,resulting from activated peroxidase and b-1,3-glucanase after B.sorokiniana infection,and reactive oxygen species-related genes were upregulated in Ta TLP1-OE lines after Pt infection.These results indicated that stable expression of TaTLP1 increased resistance against both diseases.Zhongchi Cui Fang Liang Jiarui Zhang Fei Wang Daqun Liu Haiyan Wang 2021The Crop Journal2021,9,5:3
2Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671显示文摘Huixin Zhou Xianchun Xia Zhonghu He Xing Li Cuifen Wang Zaifeng Li Daqun Liu 2013Theoretical and Applied Genetics2013,,8:2
3High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis显示文摘Dear Editor,Wheat is one of the most important staple food crops.Leaf rust caused by Puccinia triticina Eriks.(Pt)is a destructive foliar disease that threatens world wheat production.Complementary wheat genes Necrosis 1(Ne1)and Necrosis 2(Ne2)on chromosome arms 5BL and 2BS,respectively,cause hybrid necrosis(Caldwell and Compton,1943;Chu et al.,2006).Ne2 shows extremely tight genetic linkage with leaf rust resistance gene Lr13(Zhang et al.,2016).We previously identified a temperature-sensitive leaf rust resistance gene LrZH22 in Chinese wheat cultivar Zhoumai 22(ZM,Figure 1A)that was effective against most Chinese Pt pathotypes(Wang et al.,2016)and shared an overlapping genetic interval with Lr13.In a separate study,a recessive early leaf senescence 1(els1)gene(Figure 1B)shared an overlapping interval with LrZH22/Lr13/Ne2(Chu et al.,2006;Wang et al.,2016;Zhang et al.,2016;Li et al.,2018).However,the function and relationship of LrZH22/Lr13/Ne2/els1 remained unknown for decades.Here,we report the map-based cloning of LrZH22 and els1 and characterize their relationship with Lr13 and Ne2 in conjunction with a companion paper(Hewitt et al.t 2021)studying the same gene.Xiaocui Yan Miaomiao Li Peipei Zhang Guihong Yin Huaizhi Zhang Takele Weldu Gebrewahid Jianping Zhang Lingli Dong Daqun Liu Zhiyong Liu Zaifeng Li 2021Molecular Plant2021,14,7:2
4Effects of electric-acoustic and acoustic-electric conversions of transducers on acoustic logging signal显示文摘Based on an acoustic logging transmission network and the engineering pattern of a sliding wave in acoustic logging, analysis and calculations have been performed in a study of the effects of the electric-acoustic and acoustic-electric conversions of the transducers on the acoustic logging signal. The results show that acoustic-electric conversion through the transducer can cause not only a serious disturbance in the signal amplitude, but also an apparent transmission delay. For engineering applications, the amplitude variation and transmission delay must be accounted for in a practical analysis of the acoustic logging signal in rocks. The results also show that with enhanced understanding and proper justification, the error caused by the acoustic-electric conversion can be significantly reduced in evaluation of the cement bond quality of a cased well, and the accuracy of rock porosity calculated using the measured acoustic velocity can be increased.FA Lin XIE WenYan TIAN Yong ZHAO MeiShan MA Li DONG DaQun 2012Chinese Science Bulletin2012,57,11:2
5QTL mapping of adult plant resistance to stripe rust and leaf rust in a Fuyu 3/Zhengzhou 5389 wheat population显示文摘Stripe or yellow rust(YR)and leaf rust(LR)cause large losses in wheat production worldwide.Resistant cultivars curtail the levels of losses.The present study aimed to identify quantitative trait loci(QTL)for YR and LR resistance in 147 F2:6 recombinant inbred lines(RIL)derived from the cross Fuyu 3/Zhengzhou 5389.The RIL population and parents were genotyped with the Wheat55 K single nucleotide polymorphism(SNP)array and simple sequence repeat(SSR)markers.All materials were also phenotyped for YR severity at Mianyang in Sichuan province and Baoding in Hebei province in the 2015/2016,2016/2017,and 2017/2018 cropping seasons,and LR severity at Zhoukou in Henan province and at Baoding in 2017/2018.Eleven QTL for YR resistance and five for LR resistance were detected using inclusive composite interval mapping(Ici Mapping).Four of these QTL on chromosomes 1 BL,2 BS,3 AL,and 5 AL conferred resistance to both YR and LR.The QTL on 1 BL was Lr46/Yr29,and that on 7 BL might be Lr68.The QTL on chromosome 2 BS was detected at a similar position to previously detected loci.QYr.hebau-3 AL/QLr.hebau-3 AL,QYr.hebau-5 AL/QLr.hebau-5 AL,QYr.hebau-7 DL,QYr.hebau-4 BS,QYr.hebau-6 DL,and QYr.hebau-2 AS are likely to be new.An SSR marker for QYr.hebau-7 DL was developed and validated in a diverse wheat panel from China,suggesting effectiveness in different genetic backgrounds.These QTL with closely linked SNP and SSR markers could be useful for marker-assisted selection in wheat breeding programs targeting durable resistance to both diseases.Takele Weldu Gebrewahid Peipei Zhang Yue Zhou Xiaocui Yan Xianchun Xia Zhonghu He Daqun Liu Zaifeng Li 2020The Crop Journal2020,8,4:2
6Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900显示文摘Lifang Xing Cuifen Wang Xianchun Xia Zhonghu He Wanquan Chen Taiguo Liu Zaifeng Li Daqun Liu 2014Molecular Breeding2014,,4:1
7An AFLP marker linked to the leaf rust resistanc gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL显示文摘Leaf rust(LR), caused by Puccinia triticina, is one of the most widespread diseases of common wheat(Triticum aestivum L.) worldwide. The LR resistance gene Lr Bi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with genetic distances of 2.8 c M and 2.9 c M, respectively. In the present study, a total of 304 AFLP primer pairs were used to screen Bimai 16 and Thatcher and resistant and susceptible DNA bulks. The polymorphic AFLP marker P-ATT/M-CGC173 bp was used to genotype F2 and F3populations to identify markers more closely linked to Lr Bi16. Marker P-ATT/M-CGC173 bp was tightly linked to Lr Bi16 with a genetic distance of0.5 c M. As Lr Bi16 was mapped near the Lr14 a locus, 809 F2 plants from the Bimai 16/RL6013(Lr14a) cross were inoculated with the Pt pathotype FHNQ to test the allelism of Lr14 a and Lr Bi16. All of the F2 plants were resistant to FHNQ(IT between; and 2), suggesting that Lr14 a and Lr Bi16 are allelic.Peipei Zhang Huixin Zhou Caixia Lan Zaifeng Li Daqun Liu 2015The Crop Journal2015,3,2:1
8Phylogenetic diversi- ty of culturable bacteria from alpine permafrost in the Tianshan Mountains, northwestern China显示文摘Bai Yu Yang Daqun Wang Jianhui 2006Research in Microbiology2006,157,8:1
9Two-level DEA approaches in research evaluation显示文摘Meng Wei Zhang Daqun Qi Li 2008Omega2008,36,6:1
10Puccinia triticina effector protein Pt_21 interacts with wheat thaumatin-like protein TaTLP1 to inhibit its antifungal activity and suppress wheat apoplast immunity显示文摘Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effector-mediated wheat susceptibility remains largely unexplored. In this study, an effector Pt_21 was identified to interact with the apoplast-localized wheat thaumatin-like protein TaTLP1 using a yeast two-hybrid assay and the Pt_21-TaTLP1 interaction was characterized. The interaction between Pt_21 and TaTLP1 was validated by in vivo co-immunoprecipitation assay. A TaTLP1 variant,TaTLP1C71A, that was identified by the site-directed mutagenesis failed to interact with Pt_21. Pt_21was able to suppress Bax-mediated cell death in leaves of Nicotiana benthamiana and inhibit TaTLP1-mediated antifungal activity. Furthermore, infiltration of recombinant protein Pt_21 into leaves of transgenic wheat line overexpressing TaTLP1 enhanced the disease development of leaf rust compared to that in wild-type leaves. These findings demonstrate that Pt_21 suppresses host defense response by directly targeting wheat TaTLP1 and inhibiting its antifungal activity, which broadens our understanding of the molecular mechanisms underlying Pt effector-mediated susceptibility in wheat.Fei Wang Songsong Shen Zhongchi Cui Shitao Yuan Ping Qu Hui Jia Linshuo Meng Xiaoyu Hao Daqun Liu Lisong Ma Haiyan Wang 2023The Crop Journal2023,11,5:0
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