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| 1 | Differential expression of genes during aflatoxin B_1-induced hepatocarcinogenesis in tree shrews显示文摘AIM:Through exploring the regulation of gene expression during hepatocarcinogenesis induced by aflatoxin B1 (AFB1),to find out the responsible genes for hepatocellular carcinoma (HCC) and to further understand the underlying molecular mechanism.METHODS:Tree shrews ( Tupaia belangeri chinensis)were treated with or without AFB1 for about 90 weeks. Liver biopsies were performed regularly during the animal experiment. Eight shares of total RNA were respectively isolated from 2 HCC tissues, 2 HCC-surrounding noncancerous liver tissues, 2 biopsied tissues at the early stage(30^th week) of the experiment from the same animals as above, 1 mixed sample of three liver tissues biopsied at the beginning (0^th week) of the experiment, and another i mixed sample of two liver tissues from the untreated control animals biopsied at the 90^th week of the experiment. The samples were then tested with the method of Atlas^TM cDNA microarray assay. The levels of gene expression in these tissues taken at different time points during hepatocarcinogenesis were compared.RESULTS:The profiles of differently expressed genes were quite different in different ways of comparison.At the same period of hepatocarcinogenesis, the genes in the same function group usually had the same tendency for up-or down-regulation. Among the checked 588 genes that were known to be related to human cancer, 89 genes (15.1%) were recognized as “important genes” because they showed frequent changes in different ways of comparison. The differentially expressed genes during hepatocarcinogenesis could be classified into four categories: genes up-regulated in HCC tissue, genes with similar expressing levels in both HCC and HCC-surrounding liver tissues which were higher than that in the tissues prior to the development of HCC,genes down-regulated in HCC tissue, and genes up-regulated prior to the development of HCC but down-regulated after the development of HCC.CONCLUSION: A considerable number of genes could change their expressing levels both in HCC and in HCC-surrounding non-cancerous liver tissues. A few modular genes were up-regulated only in HCC but not in surrounding liver tissues, while some apoptosis-related genes were down-regulated in HCC and up-regulated in surrounding liver tissues. To compare gene-expressing levels among the liver tissues taken at different time points during hepatocarcinogenesis may be helpful to locate the responsible gene (s) and understand the mechanism for AFB1 induced liver cancer. | YuanLi Da-FangWan Jian-JiaSu Jicao ChaoOu Xiao-KunQiu Ke-ChenBan ChunYang Liu-LiangQin DanLuo Hui-FenYue Li-ShengZhang Jian-RenGu | 2004 | World Journal of Gastroenterology2004，10，4： | 11 |
| 2 | Clinical Evaluation of Color Doppler Ultrasound in Selecting the Optimal Treatment Modality for Infantile Hemangioma显示文摘客观我们调查了 1064 nm Nd 的功效和安全:钇铝柘榴石激光,强烈的搏动的光( IPL ),和在 hemangioma 的治疗的 lauromacrogol 注射,作为 hemangiomas 诊断了以便在选最佳的治疗 modality.Methods 为临床的婴儿的病人评估颜色 Doppler 超声指导的价值随机被划分成组 A ,谁在治疗,和组 B 前有颜色 Doppler 超声考试没有超声评估,有治疗。在组 A 的病人被 sonography 根据损害的深度分配进亚群:为有损害深度的那些的组 A-1 < 1.2 公里,并且诊脉轻治疗;为有损害深度 1.2mm 的那些的组 A-2 并且 < 3 公里,并且诊脉 1064 nm Nd : 钇铝柘榴石激光治疗;为有损害深度 3mm 的那些的组 A-3 并且 < 5 公里,并且被与长脉搏相结合的 IPL 对待 1064 nm Nd : 钇铝柘榴石激光治疗;为有损害深度的那些的组 A-4 5 公里,并且拿了 lauromacrogol 注射治疗。在组 B 的病人诊脉 1064 nm Nd : 没有外科手术前的超声评估的钇铝柘榴石激光治疗。功效和在这些组之间的治疗的不利反应完全被评估并且比较 statistically.Results 有 128 皮肤损害的 113 个病人在这研究被注册, 85 在组 A (吝啬的年龄 6.8 吗?? | MinLi JuanLiu ValeskaMaya DanLuo Bingrong Zhou | 2017 | Chinese Medical Sciences Journal2017，32，2： | 6 |
| 3 | Enhancement of transfection by physial concentration of DNA at the cell surface 显示文摘 | Danluo Mark S | 2000 | Nat Biot2000，13，： | 1 |
| 4 | Rapid magnetic solid-phase extraction based on magnetite/silica/poly ( methacrylic acid - co - ethylene glycol dimethacrylate ) composite microspheres for the determination of sulfonamide in milk samples 显示文摘 | Qiang Gao DanLuo Jun Ding | 2010 | Journal of Chromatography A2010，1217，35： | 1 |
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