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| 1 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 2 | Simulation research on the influence of stress relaxation on plastic IC delamination during scrapped PCB disassembly 显示文摘 | LONG Danfeng XIANG Dong DONG Yehong | 2011 | International Journal of Environmental Technology and Management2011，14，56： | 1 |
| 3 | Identification of two potential glycogen synthase kinase 3β inhibitors for the treatment of osteosarcoma显示文摘Osteosarcoma 是在与高死亡率世界范围的青少年之中的最普通的主要恶意的骨头肿瘤。肝糖 synthase kinase (GSK3 ) 3 是 serine/threonine kinase 并且在 osteosarcoma 被看作一个验证目标治疗。因此, GSK3 禁止者的学习是在 anti-osteosarcoma 药开发的最流行的领域之一。这里,生物信息学的工具被用来从锌药数据库屏蔽 GSK3 的新奇有效禁止者。分子的停靠、分子的动态模拟, MM/GBSA,和精力分解分析被执行识别禁止者。最后, ZINC08383479 和 ZINC08441251 作为潜在的 GSK3 禁止者被选择。这二个禁止者被 GSK3 kinase 抑制试金在 vitro 评估。房间增长的抑制在 vitro 在 osteosarcoma 房间线 U2OS 和 MG63 被测试。结果显示出那 ZINC08383479, ZINC08441251 对 GSK3 举办了高抑制活动。我们发现 CHIR99021 (一个已知的 GSK3 禁止者) , ZINC08383479,和 ZINC08441251 在 U2OS 房间和 MG63 房间举办了重要抑制活动。这些调查结果可以为 osteosarcoma 为更多的有势力 GSK3 禁止者和治疗学的目标的设计提供新想法。 | Kaimin Lu Xin Wang Yuyu Chen Danfeng Liang Hao Luo Li Long Zongyue Hu Jinku Bao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，5： | 0 |
| 4 | Improved suspension quality and liquid level stability in stirred tanks with Rotor-Stator agitator based on CFD simulation显示文摘The solid-liquid mixing is an important operation unit in the preparation of composites by stirring casting.High quality composite materials need good homogeneity and stable liquid level.In this work,the performances of the Rotor-Stator agitator for solid suspension in stirred tank were investigated through CFD modeling,including the homogeneity,power consumption and liquid level stability.The Eulerian-Eulerian(E-E)multiphase model and the RNG k-εturbulence model were adopted for modeling the multiphase flow and the turbulence effects,respectively.The effect of various important parameters such as stirring speed,particle size,solid loading and the use of baffles were examined.Adding a stator structure with array holes outside the rotor can optimize the flow pattern,thereby improving the mixing uniformity and liquid level stability.The homogeneity and liquid level stability attained by the Rotor-Stator agitator were better than those for the A200(an axial-flow agitator)and the Rushton(a radial-flow agitator). | Cuicui Yin Jiazhen He Yongnan Xiong Zhibo Zhang Xing Luo Yao Shu Danfeng Long Kaihong Zheng | 2023 | Particuology2023，，11： | 0 |
| 5 | ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development显示文摘Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation. | Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei Tingwei Yan Yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu Yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-NanYang Xiangyuan Wan | 2023 | Molecular Plant2023，16，8： | 0 |
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