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| 1 | Preventive effect of glutamine on intestinal barrier dysfunction induced by severe trauma显示文摘AIM: To investigate the mechanism underlying intestinalbarrier function damage after severe trauma and thetherapeutic effect of glutamine.METHODS: Burned patients, and animal models of severstrauma replicated by hemorrhagic shock combined withendotoxin infusion and burn injury, were included in a serialexperiment. Effects of oral glutamine on intestinal barrierfunction were observed in scalded rets. Parametersmeasured in these experiments were as follows: plasmalevels of diamine oxidase (DAO), tumor necrosis factor(TNFα), endotoxin (LPS), and lactate as well as D-lactateby biochemical methods, lactose/mannitol (L/M) ratio inurine by SP-3400, and pathological examination of intestinalmucosa under light microscopy.RESULTS: Plasma DAO activity was significantly increasedafter injury. There was a negative correlation betweenplasma DAO and intestinal mucosal DAO or pHi ( r= -0.93,plasma 0.80 ± 0.93,2.83 ± 1.71, 1.14 ± 0.64,2.36 ± 2.06 and2.49± 1.67 vs intestinal 0.52± 0.12,0.34 ± 0.03,0.45 ± 0.18,0.37± 0.26 and 0.41 ± 0.07; r = - 0.533, plasma 0.87 ± 0.75,1.89± 1.13, 1.21 ± 0.23,3.03 ± 2.61 and 4.70 ± 1.22 Vs pHi7.03± 0.05,7.05 ± 0.06,7.14 ± 0.096,7.20 ± 0.08 and 7.05 ±0.07; P < 0.01-0.05). Positive correlations were foundbetween DAO activity and plasma TNFα, LPS, lactate, L/Mand D-lactate ( r = 0.817, 0.842, 0.872, and 0.951; plasmaDAO 0.87 ± 0.75,1.89 ± 1.13, 1.21 ± 0.23,3.03 ± 2.61 and 4.70± 1.22 vs TNF 0.08 ± 0.02,0.03 ± 0.25,0.17 ± 0.09,0.34 ± 0.15and 0.33 ± 0.18; vs LPS 0.14 ± 0.03,0.16 ± 0.04,0.21 ± 0.02,0.18± 0.16 and 0.37 ± 0.10; vs lactate 9.03 ± 2.19, 18.30 ±2.56,9.81 ± 2.83,12.01 ± 6.83, 12.01 ± 6.84 and 43.61 ± 11.27;vs L/M 0.03 ± 0.01,0.41 ± 0.27,0.62 ± 0.20, 1.70 ± 0.60; r =0.774, plasma DAO 1.25 ± 0.41,2.17 ± 0.71,2.29 ± 0.87, 1.23± 0.55 and 1.11 ± 0.47 vs D-lactate 8.37 ± 2.48, 18.25 ± 6.18,13.96 ± 4.94, 8.93 ± 3.00 and 12.39 ± 4.94; all P < 0.01),repestively. Damage of intestinal mucosa was found bypathological examination. Intestinal barrier function wasimproved to a certain extent by oral glutamine in scaldedrats.CONCLUSION: Intestinal barrier function was damaged in theearly stage after trauma. Plasma DAO activity, D-lactatecontent, intestinal pHi and urine L/M may be sensitivemarkers of intestinal mechanical injury, and glutamine mayprotect against intestinal barrier dysfunction after severetrauma. | Jun-You Li Yi Lu Sen Hu Dan Sun Yong-Ming Yao Burn Institute,Chinese PLA 304 Hospital,Beijing 100037,China | 2002 | World Journal of Gastroenterology2002，8，1： | 74 |
| 2 | Effects of glycyrrhetinic acid on collagen metabolism of hepatic stellate cells at different stages of liver fibrosis in rats显示文摘INTRODUCTIONLiver fibrosis is a dynamic course leading tocirrhosis from a various chronic liver diseases. Thepathological basis of fibrosis is the disturbance ofproduction and degradation of the extracellularmatrix (ECM), which causes accumulation of ECMin the liver[1,2]. | Ji Yao Wang Qi Sheng Zhang Ji Sheng Guo Mei Yu Hu Department of Gastroenterology, Zhongshan Hospital, Medical Center, Fu Dan University Shanghai Medical University), Shanghai 200032, China | 2001 | World Journal of Gastroenterology2001，7，1： | 29 |
| 3 | Ratios of T-helper 2 Cells to T-helper 1 Cells and Cytokine Levels in Patients with Hepatitis B显示文摘 | Ming-Hui Li Dan Zhang Lu Zhang Xiao-Jing Qu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Hong-Xiao Hao Wen-Hao Hua Shu-Jing Song Gang Wan Shun-Ai Liu Yao Xie | 2017 | Chinese Medical Journal2017，，15： | 21 |
| 4 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 5 | Association of Cytokines with Alanine Aminotransferase, Hepatitis B Virus Surface Antigen and Hepatitis B Envelope Antigen Levels in Chronic Hepatitis B显示文摘 | Ming-Hui Li Yao Lu Lu Zhang Xing-Yue Wang Chong-Ping Ran Hong-Xiao Hao Dan Zhang Xiao-Jing Qu Ge Shen Shu-Ling Wu Wei-Hua Cao Tian-Lin Qi Ru-Yu Liu Lei-Ping Hu Min Chang Wen-Hao Hua Shun-Ai Liu Gang Wan Yao Xie | 2018 | Chinese Medical Journal2018，，15： | 17 |
| 6 | Plasmacytoid Dendritic Cell Function and Cytokine Network Profiles in Patients with Acute or Chronic Hepatitis B Virus Infection显示文摘 | Ming-Hui Li Lu Zhang Dan Zhang Wei-Hua Cao Tian-Lin Qi Hong-Xiao Hao Xing-Yue Wang Chong-Ping Ran Xiao-Jing Qu Shun-Ai Liu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Wen-Hao Hua Gang Wan Jun Cheng Yao Xie | 2018 | Chinese Medical Journal2018，，1： | 16 |
| 7 | Tissue-specific alterations in expression and function of P-glycoprotein in streptozotocin-induced diabetic rats显示文摘瞄准: 在导致 streptozocin 的糖尿病的老鼠的服的外皮,马头鱼尾的怪兽,肝,肠的 mucosa 和肾调查表达式的变化和 P-glycoprotein (P-GP ) 的功能。 | Lu-lu ZHANG Liang LU Shi JIN Xin-yue JING Dan YAO Nan HU Li LIU Ru DUAN Xiao-dong LIU Guang-ji WANG Lin XIE | 2011 | Acta Pharmacologica Sinica2011，32，7： | 15 |
| 8 | The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge Genome Evolution and Rubber Biosynthesis显示文摘The rubber tree,Hevea brasiliensis,produces natural rubber that serves as an essential industrial raw material.Here,we present a high-quality reference genome for a rubber tree cultivar GT1 using single-molecule real-time sequencing(SMRT)and Hi-C technologies to anchor the~1.47-Gb genome assembly into 18 pseudochromosomes.The chromosome-based genome analysis enabled us to establish a model of spurge chromosome evolution,since the common paleopolyploid event occurred before the split of Hevea and Manihot.We show recent and rapid bursts of the three Hevea-specific LTR-retrotransposon families during the last 10 million years,leading to the massive expansion by~65.88%(~970 Mbp)of the whole rubber tree genome since the divergence from Manihot.We identify large-scale expansion of genes associated with whole rubber biosynthesis processes,such as basal metabolic processes,ethylene biosynthesis,and the activation of polysaccharide and glycoprotein lectin,which are important properties for latex production.A map of genomic variation between the cultivated and wild rubber trees was obtained,which contains~15.7 million high-quality single-nucleotide polymorphisms.We identified hundreds of candidate domestication genes with drastically lowered genomic diversity in the cultivated but not wild rubber trees despite a relatively short domestication history of rubber tree,some of which are involved in rubber biosynthesis.This genome assembly represents key resources for future rubber tree research and breeding,providing novel targets for improving plant biotic and abiotic tolerance and rubber production. | Jin Liu Cong Shi Cheng-Cheng Shi Wei Li Qun-Jie Zhang Yun Zhang Kui Li Hui-Fang Lu Chao Shi Si-Tao Zhu Zai-Yun Xiao Hong Nan Yao Yue Xun-Ge Zhu Yu Wu Xiao-Ning Hong Guang-Yi Fan Yan Tong Dan Zhang Chang-Li Mao Yun-Long Liu Shi-Jie Hao Wei-Qing Liu Mei-Qi Lv Hai-Bin Zhang Yuan Liu Ge-Ran Hu-tang Jin-Peng Wang Jia-Hao Wang Ying-Huai Sun Shu-Bang Ni Wen-Bin Chen Xing-Cai Zhang Yuan-Nian Jiao Evan E.Eichler Guo-Hua Li Xin Liu Li-Zhi Gao | 2020 | Molecular Plant2020，13，2： | 15 |
| 9 | Genetic and Epigenetic Alterations of DLC-1,a Candidate Tumor Suppressor Gene,in Nasopharyngeal Carcinoma显示文摘DLC-1 基因,在人的染色体区域 8p22 定位了,象一个肿瘤一样表现压制或基因并且经常在多样的肿瘤被删除。8p22 的删除不是在鼻咽的癌(NPC ) 的一个不平常的事件,因此,我们由反向的抄写聚合酶链反应在 NPC 和 NPC 房间线探索了 DLC-1 基因的表示层次。结果证明 DLC-1 的 mRNA 水平低调整。为了识别 DLC-1 的机制,在 NPC 击倒规定,我们用methylation特定的 PCR 调查了 DLC-1 基因的 methylation 地位,并且发现那79%( 39 中的 31 个)非 NPC 纸巾和二 DLC-1 表示 NPC 房间线, 6-10B 和 5-8F ,是在 DLC-1 CpG 岛的 methylated 。 Microsatellite PCR 也被执行,并且杂合现象的损失在用33%的比率( 12 个增进知识的案例中的 4 个)盖住整个 DLC-1 基因的四个微卫星地点( D8S552 , D8S1754 , D8S1790 和 D8S549 )被发现,18%( 11 中的 2 个),5%( 18 中的 1 个),并且25%( 12 中的 3 个)分别地。总起来说,我们的结果建议 DLC-1 可能是一个 NPC 相关的肿瘤压制或异常倡导者 methylation 和基因删除影响的基因。 | Dan PENG Cai-Ping REN Hong-Mei YI Liang ZHOU Xu-Yu YANG Hui LI Kai-Tai YAO | 2006 | Acta Biochimica et Biophysica Sinica2006，38，5： | 14 |
| 10 | Structured illumination microscopy for super-resolution and optical sectioning显示文摘Optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact,minimally invasive observation and measurement of live specimens.However,the conventional optical microscopy only has a spatial resolution about200 nm due to the Abbe diffraction limit,and also lacks the ability of three-dimensional imaging.Super-resolution farfield optical microscopy based on special illumination schemes has been dramatically developed over the last decade.Among them,only the structured illumination microscopy(SIM)is of wide-field geometry that enables it easily compatible with the conventional optical microscope.In this article,the principle of SIM was introduced in terms of point spread function(PSF)and optical transform function(OTF)of the optical system.The SIM for super-resolution(SIM-SR)proposed by Gustafsson et al.and the SIM for optical sectioning(SIM-OS)proposed by Neil et al.are the most popular ones in the research community of microscopy.They have the same optical configuration,but with different data postprocessing algorithms.We mathematically described the basic theories for both of the SIMs,respectively,and presented some numerical simulations to show the effects of super-resolution and optical sectioning.Various approaches to generation of structured illumination patterns were reviewed.As an example,a SIM system based on DMDmodulation and LED-illumination was demonstrated.A lateral resolution of 90 nm was achieved with gold nanoparticles.The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons,and the sectioning strength of 930 nm was obtained. | Dan Dan Baoli Yao Ming Lei | 2014 | Chinese Science Bulletin2014，59，12： | 14 |
| 11 | Eleven COVID-19 Outbreaks with Local Transmissions Caused by the Imported SARS-CoV-2 Delta VOC-China,July-August,2021显示文摘Starting from December 2019,Wuhan,China,encountered the first outbreak of coronavirus disease 2019(COVID-19)(1-2).The epidemic was successfully suppressed by strict containment so that the number of infected people was reduced to 0 on April 8,2020(3–4).After that,China experienced roughly 3 dozen outbreaks with local transmission caused by imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Lei Zhou Kai Nie Hongting Zhao Xiang Zhao Bixiong Ye Ji Wang Cao Chen Hong Wang Jiangli Di Jinsong Li Chao Li Zhixiao Chen Ruiqi Ren Peihua Niu Hui Chen Tao Chen Yali Wang Lili Li Bingxue Han Ruhan A Si Chen Dan Li Dan Li Chunxia Jin Xin Zhang Jiangmei Liu Chunyu Xu Yecheng Yao Yenan Feng Zhili Li Bike Zhang Yang Song Peter Hao Yanping Zhang Hao Li Qun Li George F.Gao Guoqing Shi Wenbo Xu | 2021 | China CDC weekly2021，3，41： | 13 |
| 12 | Differentiating between Alzheimer’s disease, amnestic mild cognitive impairment, and normal aging via diffusion kurtosis imaging显示文摘Diffusion kurtosis imaging can be used to assess pathophysiological changes in tissue structure and to diagnose central nervous system diseases. However, its sensitivity in assessing hippocampal differences between patients with Alzheimer’s disease and those with amnestic mild cognitive impairment has not been characterized. Here, we examined 20 individuals with Alzheimer’s disease (11 men and 9 women, mean 73.2 ± 4.49 years), 20 with amnestic mild cognitive impairment (10 men and 10 women, mean 71.55 ± 4.77 years), and 20 normal controls (11 men and 9 women, mean 70.45 ± 5.04 years). We conducted diffusion kurtosis imaging, using a 3.0 T magnetic resonance scanner, to compare hippocampal differences among the three groups. The results demonstrated that the right hippocampal volume and bilateral mean kurtosis were remarkably smaller in individuals with Alzheimer’s disease compared with those with amnestic mild cognitive impairment and normal controls. Further, the mean kurtosis was lower in the amnestic mild cognitive impairment group compared with the normal control group. The mean diffusion in the left hippocampus was lower in the Alzheimer’s disease group than in the amnestic mild cognitive impairment and normal control groups, while the mean diffusion in the right hippocampus was lower in the Alzheimer’s disease group than in the normal control group. Fractional anisotropy was similar among the three groups. These results verify that bilateral mean kurtosis and mean diffusion are sensitive to the diagnosis of Alzheimer’s disease and amnestic mild cognitive impairment. This study was approved by the Ethics Review Board of Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, China on May 4, 2010 (approval No. 2010(C)-6). | Guo-Ping Song Ting-Ting Yao Dan Wang Yue-Hua Li | 2019 | Neural Regeneration Research2019，14，12： | 11 |
| 13 | Herbal medicine Yin Zhi Huang induces CYP3A4-mediated sulfoxidation and CYP2C19-dependent hydroxylation of omeprazole显示文摘瞄准:探索在殷·智·胡昂(YZH ) 和 omeprazole 之间的潜在的相互作用, CYP3A4 和 CYP2C19 的底层。方法:十八个健康志愿者包括 6 CYP2C19 *1 /*1, 6 CYP2C19 *1 /*2 和 6 CYP2C19 *2 /*2 在 2 阶段被注册,使随机化,转线路临床的试用。在每个阶段,志愿者收到了安慰剂或 10 mL YZH 口头的液体,为 14 d 3 倍每日。然后,所有病人口头上地拿了 20 mg omeprazole 囊。血样品在 omeprazole 管理以后被收集直到 12 h。omeprazole 和它的代谢物的血浆集中被 HPLC 与紫外察觉确定。结果:在 YZH 的治疗的 14 d 以后,血浆 omeprazole 显著地减少了,那些显著地 omeprazole 砜和 5-hydroxyomeprazole 增加了。在从到无穷的时间 0 的血浆集中时间曲线下面的区域的比率(到到 omeprazole 砜的 omeprazole 的 5-hydroxyomprazole 和那些的 omeprazole 的 AUC (0无穷)在 CYP2C19 *1 / *1 由64.80%+/-12.51%( P=0.001 )和63.31%+/-18.45%( P=0.004 )减少了,57.98%+/-14.80%( P=0.002 )和在 CYP2C19 *1 / *2 或 *3 的54.87%+/-18.42%( P=0.003 ),和37.74%+/-16.07%( P=0.004 )和在 CYP2C19 *2 / *2 的45.16%+/-15.54%( P=0.003 )分别地。到在 CYP2C19 *1 /*1 和 CYP2C19 *1 /*2 或 *3 的 5-hydroxyomprazole 的 omeprazole 的 AUC (0 无穷) 比率的减少比在 CYP2C19 *2 /*2 (P=0.047 和 P=0.009 ) 的那些大。结论:YZH 导致催化 CYP3A4 的 sulfoxidation 和在血浆 omeprazole 集中导致减少的 omeprazole 的 CYP2C19 依赖的 hydroxylation。 | Lan FAN Guo WANG Lian-sheng WANG Yao CHEN Wei ZHANG Yuan-fei HUANG Rui-xue HUANG Dong-li HU Dan WANG Hong-hao ZHOU | 2007 | Acta Pharmacologica Sinica2007，28，10： | 10 |
| 14 | Characteristics of interior noise of a Chinese high-speed train under a variety of conditions显示文摘目的:研究高速列车在不同速度(260~385 km/h)、无砟和有砟轨道以及明线和隧道运行时的车内噪声特性,为高速列车车内减振降噪和车体低噪声设计提供科学依据。创新点:系统分析高速列车在不同运行条件下的车内噪声总值变化、空间分布、频谱特性和声源贡献,掌握车内噪声随列车运行速度的变化规律、轨道型式和隧道混响对车内噪声的影响,研究轮轨噪声、气动噪声和弓网噪声对车内噪声的作用。方法:1.根据不同的列车运行速度,分析车内噪声的变化规律;通过进一步对比头尾车运行时,不同测点位置的噪声总值、显著频率和声源贡献,研究气动作用对车内噪声的影响。2.针对不同轨道型式,分析车内噪声的差异特性;通过进一步对比不同速度下的前、后转向架上方车内噪声测点(其中一个还位于受电弓下方)的噪声总值、显著频率和声源贡献,研究有砟和无砟轨道、弓网噪声对车内噪声的影响以及速度因素的作用。3.对于隧道运行,分析不同速度下在明线和隧道运行时的车内噪声总值和显著频率,研究隧道混响对车内噪声的影响以及速度因素的作用。结论:1.TC01车无论作为头车或尾车运行,车内观光区的噪声均主要受气动作用影响。2.随着列车运行速度的提高,轨道型式的不同对车内噪声的影响有所降低。3.隧道混响对中间车的影响要高于头车。 | Jie ZHANG Xin-biao XIAO Xiao-zhen SHENG Rong FU Dan YAO Xue-song JIN | 2017 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2017，18，8： | 10 |
| 15 | Comparative analysis of the complete chloroplast genome among Prunus mume,P.armeniaca,and P.salicina显示文摘Prunus mume Sieb.et Zucc.,P.armeniaca L.,and P.salicina L.are economically important fruit trees in temperate regions.These species are taxonomically perplexing because of shared interspecific morphological traits and variation,which are mainly attributed to hybridization.The chloroplast is cytoplasmically inherited and often used for evolutionary studies.We sequenced the complete chloroplast genomes of P.mume,P.armeniaca,and P.salicina using Illumina sequencing followed by de novo assembly.The three chloroplast genomes exhibit a typical quadripartite structure with conserved genome arrangement,structure,and moderate divergence.The lengths of the genomes are 157,815,157,797,and 157,916 bp,respectively.The length of the large single-copy region(LSC)region is 86,113,86,283,and 86,122 bp,and the length of the SSC region is 18,916,18,734,and 19,028 bp;the IR region is 26,393,26,390,and 26,383 bp,respectively.Each of the three chloroplast genomes encodes 133 genes,including 94 protein-coding,31 tRNA,and eight rRNA genes.Differential gene analysis for the three species revealed that trnY-ATA is a unique gene in P.armeniaca;in contrast,the gene trnI-TAT is only present in P.mume and P.salicina,though the position of the gene in these chloroplast genomes differs.Further comparative analysis of the complete chloroplast genome sequences revealed that the ORF genes and the sequences of linked regions rps16 and atpA,atpH and atpI,trnc-GCA and psbD,ycf3 and atpB,and rpL32 and ndhD are significantly different and may be used as molecular markers in taxonomic studies.Phylogenetic evolution analysis of the three species suggests that P.mume has a closer genetic relationship to P.armeniaca than to P.salicina. | Song Xue Ting Shi Wenjie Luo Xiaopeng Ni Shahid Iqbal Zhaojun Ni Xiao Huang Dan Yao Zhijun Shen Zhihong Gao | 2019 | Horticulture Research2019，6，1： | 10 |
| 16 | Improved normalization method for ductile fracture toughness determination based on dimensionless load separation principle显示文摘This study successfully deals with the inhomogeneous dimension problem of load separation assumption, which is the theoretical basis of the normalization method. According to the dimensionless load separation principle, the normalization method has been improved by introducing a forcible blunting correction. With the improved normalization method, the J-resistance curves of five different metallic materials of CT and SEB specimens are estimated. The forcible blunting correction of initial crack size plays an important role in the J-resistance curve estimation, which is closely related to the strain hardening level of material. The higher level of strain hardening leads to a greater difference in JQ determined by different slopes of the blunting line.If the blunting line coefficient recommended by ASTM E1820-11 is used in the improved normalization method, it will lead to greater fracture resistance than that processed by the blunting line coefficient recommended by ISO 12135-2002. Therefore, the influence of the blunting line on the determination of JQ must be taken into full account in the fracture toughness assessment of metallic materials. | Chen Bao Lixun Cai Kaikai Shi Chen Dan Yao Yao | 2015 | Acta Mechanica Solida Sinica2015，28，2： | 10 |
| 17 | Investigation into the Formation, Structure, and Evolution of an EF4 Tornado in East China Using a High-Resolution Numerical Simulation显示文摘Devastating tornadoes in China have received growing attention in recent years, but little is known about their formation, structure, and evolution on the tornadic scale. Most of these tornadoes develop within the East Asian monsoon regime, in an environment quite different from tornadoes in the U.S. In this study, we used an idealized, highresolution(25-m grid spacing) numerical simulation to investigate the deadly EF4(Enhanced Fujita scale category 4)tornado that occurred on 23 June 2016 and claimed 99 lives in Yancheng, Jiangsu Province. A tornadic supercell developed in the simulation that had striking similarities to radar observations. The violent tornado in Funing County was reproduced, exceeding EF4(74 ms–1), consistent with the on-site damage survey. It was accompanied by a funnel cloud that extended to the surface, and exhibited a double-helix vorticity structure. The signal of tornado genesis was found first at the cloud base in the pressure perturbation field, and then developed both upward and downward in terms of maximum vertical velocity overlapping with the intense vertical vorticity centers. The tornado's demise was found to accompany strong downdrafts overlapping with the intense vorticity centers. One of the interesting findings of this work is that a violent surface vortex was able to be generated and maintained, even though the simulation employed a free-slip lower boundary condition. The success of this simulation, despite using an idealized numerical approach, provides a means to investigate more historical tornadoes in China. | Dan YAO Haile XUE Jinfang YIN Jisong SUN Xudong LIANG Jianping GUO | 2018 | Journal of Meteorological Research2018，32，2： | 10 |
| 18 | Effect of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7显示文摘Objective:To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.Methods:Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132 respectively or together.The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry,and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR.Results:Compared with the control group,the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly,and the apoptosis ratio increased distinctly(P<0.05).Besides,the affect of the co-transfection group was better than that of the single transfection group.The protein levels of GPC3(Glypican-3) and YAP(Yes-associated protein),the target genes transfected only by miR-520c-3p and miR-132,respectively,reduced obviously(P<0.05),which was similar with the co-infected cells,but cells transfected by miR-132 only showed a decrease of YAP.Conclusions:The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP,enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically. | Chang-Jiang Lei Chun Yao De-Ke Li Zhi-Xiong Long Yuan Li Dan Tao Yan-Ping Liou Jiang-Zhou Zhang Ning Liu | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，9： | 9 |
| 19 | Housing characteristics and indoor environment in relation to children's asthma, allergic diseases and pneumonia in Urumqi, China显示文摘In order to evaluate the prevalence of childhood asthma,allergic diseases and pneumonia in Urumqi City,China,as well as its associations with housing and home characteristics,a cross-sectional study was performed in 4618 children(81.7%response rate,average age 4.7±0.9 year,boys accounting for 53.7%).Questions on children’s asthma and allergic diseases were from the International Study on Asthma and Allergies in Childhood(ISAAC)and were integrated with questions on the home environment from the Dampness in Buildings and Health(DBH)study,slightly modified to account for Chinese building characteristics and life habits.The prevalences of physician diagnosed asthma,allergic rhinitis(AR)and pneumonia were 3.6%,8.7%and 40.9%,respectively.One fourth of children reported wheezing and more than 40%AR symptoms in the last 12 months.Controlling for confounding factors,positive associations were found for home mold/dampness and wheezing(adjusted odds ratio,aOR 1.33,95%CI 1.07 1.66),AR symptoms(1.34,1.09 1.64)last 12 months and physician diagnosed pneumonia(1.33,1.09 1.62).Floor material by wood,PVC or carpeting;and walls by wallpaper,painting or wood material,were positively associated with AR symptoms.Home environmental tobacco smoke(ETS)was positively associated with wheezing(1.23,1.04 1.46)and pneumonia(1.25,1.07 1.45).In conclusion,there was a relatively high prevalence of asthmatic and AR symptoms and diagnosed pneumonia in preschool children in Urumqi.Home signs of mold growth or dampness,windowpane condensation,as well as ETS and interior surface materials emitting chemicals were risk factors for allergic symptoms and pneumonia. | WANG TingTing ZHAO ZhuoHui YAO Hua WANG ShuLan NORBACK Dan CHEN Jie MA JinFeng JI XiaoLi WANG Li SUNDELL Jan | 2013 | Chinese Science Bulletin2013，58，34： | 9 |
| 20 | Location Determinants of China's Outward Foreign Direct Investment显示文摘 | Shujie Yao Fan Zhang Pan Wang Dan Luo | 2017 | China & World Economy2017，25，6： | 8 |
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